Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5849

Experiment: Nitrate reduction, replicate A

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction, replicate A
6,663,756	-		Pf6N2E2_5847	0.56	-1.2
6,663,756	-		Pf6N2E2_5847	0.56	+0.6
6,663,921	-		Pf6N2E2_5847	0.75	-0.1
6,663,939	+		Pf6N2E2_5847	0.77	-1.0
6,663,939	+		Pf6N2E2_5847	0.77	-0.3
6,663,940	-		Pf6N2E2_5847	0.77	-1.2
6,664,064	-				-0.7
6,664,113	+				+0.1
6,664,114	-				-0.5
6,664,114	-				-0.6
6,664,134	-				-1.0
6,664,147	+				-0.5
6,664,147	+				-0.3
6,664,147	+				-1.3
6,664,234	+				+1.6
6,664,235	-				+0.8
6,664,240	+				+0.9
6,664,241	-				-0.4
6,664,241	-				+0.1
6,664,269	+				+1.8
6,664,270	-				+0.1
6,664,277	+				+0.8
6,664,280	+				+0.4
6,664,280	+				+2.8
6,664,280	+				+2.0
6,664,280	+				+2.3
6,664,280	+				+1.4
6,664,280	+				+3.2
6,664,281	-				+1.4
6,664,281	-				-1.1
6,664,281	-				-0.8
6,664,281	-				-0.6
6,664,283	+				+2.8
6,664,283	+				+2.9
6,664,283	+				+1.9
6,664,283	+				+2.1
6,664,283	+				+2.3
6,664,284	-				+1.4
6,664,284	-				-3.4
6,664,284	-				-0.4
6,664,315	+				+3.3
6,664,315	+				+2.4
6,664,315	+				+2.7
6,664,315	+				+3.5
6,664,316	-				-1.6
6,664,316	-				-2.9
6,664,320	+				+3.1
6,664,335	+				+4.2
6,664,335	+				+4.5
6,664,335	+				+3.2
6,664,354	+				+0.2
6,664,377	+				-1.1
6,664,377	+				+0.1
6,664,377	+				-2.1
6,664,377	+				-0.6
6,664,470	+		Pf6N2E2_5848	0.35	-2.2
6,664,470	+		Pf6N2E2_5848	0.35	-0.6
6,664,497	+		Pf6N2E2_5848	0.43	-3.4
6,664,553	+		Pf6N2E2_5848	0.61	-0.1
6,664,554	-		Pf6N2E2_5848	0.62	-1.4
6,664,605	+		Pf6N2E2_5848	0.78	-0.7
6,664,610	-		Pf6N2E2_5848	0.79	-1.5
6,664,692	+				-1.5
6,664,692	+				-1.3
6,664,815	+				+0.1
6,664,815	+				-1.5
6,664,815	+				+0.0
6,664,815	+				-0.9
6,664,815	+				+1.4
6,664,815	+				-1.4
6,664,816	-				-2.0
6,664,817	+				-0.6
6,664,817	+				-1.2
6,664,817	+				-2.0
6,664,818	-				-0.9
6,664,818	-				-1.0
6,664,818	-				-1.1
6,664,818	-				-0.8
6,664,818	-				-1.8
6,664,818	-				-0.7
6,664,818	-				-2.5
6,664,818	-				-0.3
6,664,818	-				-0.6
6,664,826	+				+0.1
6,664,862	+		Pf6N2E2_5849	0.12	-0.4
6,664,863	-		Pf6N2E2_5849	0.12	-0.2
6,664,863	-		Pf6N2E2_5849	0.12	+1.4
6,664,865	+		Pf6N2E2_5849	0.12	-2.6
6,664,865	+		Pf6N2E2_5849	0.12	-0.3
6,664,865	+		Pf6N2E2_5849	0.12	-1.7
6,664,866	-		Pf6N2E2_5849	0.12	+2.4
6,664,866	-		Pf6N2E2_5849	0.12	-1.6
6,664,922	+		Pf6N2E2_5849	0.15	-1.5
6,664,922	+		Pf6N2E2_5849	0.15	-1.8
6,664,923	-		Pf6N2E2_5849	0.15	-0.8
6,664,923	-		Pf6N2E2_5849	0.15	-2.1
6,664,923	-		Pf6N2E2_5849	0.15	-3.6
6,664,923	-		Pf6N2E2_5849	0.15	-1.5
6,664,991	+		Pf6N2E2_5849	0.19	-1.1
6,664,991	+		Pf6N2E2_5849	0.19	-3.0
6,664,992	-		Pf6N2E2_5849	0.19	-1.3
6,664,992	-		Pf6N2E2_5849	0.19	-0.9
6,665,053	+		Pf6N2E2_5849	0.23	-2.7
6,665,054	-		Pf6N2E2_5849	0.23	+1.4
6,665,063	-		Pf6N2E2_5849	0.24	-1.5
6,665,066	+		Pf6N2E2_5849	0.24	-0.9
6,665,066	+		Pf6N2E2_5849	0.24	+0.1
6,665,066	+		Pf6N2E2_5849	0.24	-1.9
6,665,066	+		Pf6N2E2_5849	0.24	-0.2
6,665,066	+		Pf6N2E2_5849	0.24	+2.4
6,665,066	+		Pf6N2E2_5849	0.24	-2.3
6,665,067	-		Pf6N2E2_5849	0.24	-0.2
6,665,067	-		Pf6N2E2_5849	0.24	-0.9
6,665,067	-		Pf6N2E2_5849	0.24	-0.2
6,665,067	-		Pf6N2E2_5849	0.24	-1.3
6,665,067	-		Pf6N2E2_5849	0.24	-1.9
6,665,156	+		Pf6N2E2_5849	0.29	+0.1
6,665,156	+		Pf6N2E2_5849	0.29	-0.8
6,665,157	-		Pf6N2E2_5849	0.29	-2.1
6,665,157	-		Pf6N2E2_5849	0.29	-1.3
6,665,157	-		Pf6N2E2_5849	0.29	+1.4
6,665,157	-		Pf6N2E2_5849	0.29	-1.8
6,665,216	+		Pf6N2E2_5849	0.33	-0.5
6,665,216	+		Pf6N2E2_5849	0.33	+0.7
6,665,258	+		Pf6N2E2_5849	0.35	-0.6
6,665,258	+		Pf6N2E2_5849	0.35	-0.9
6,665,258	+		Pf6N2E2_5849	0.35	-1.5
6,665,258	+		Pf6N2E2_5849	0.35	-1.2
6,665,258	+		Pf6N2E2_5849	0.35	-0.9
6,665,258	+		Pf6N2E2_5849	0.35	-2.2
6,665,258	+		Pf6N2E2_5849	0.35	-3.0
6,665,258	+		Pf6N2E2_5849	0.35	-0.5
6,665,258	+		Pf6N2E2_5849	0.35	-2.0
6,665,259	-		Pf6N2E2_5849	0.36	+1.4
6,665,259	-		Pf6N2E2_5849	0.36	-1.9
6,665,259	-		Pf6N2E2_5849	0.36	-0.0
6,665,259	-		Pf6N2E2_5849	0.36	-0.8
6,665,259	-		Pf6N2E2_5849	0.36	-1.1
6,665,259	-		Pf6N2E2_5849	0.36	-0.8
6,665,259	-		Pf6N2E2_5849	0.36	-1.5
6,665,413	+		Pf6N2E2_5849	0.45	-1.4
6,665,573	+		Pf6N2E2_5849	0.54	-1.0
6,665,573	+		Pf6N2E2_5849	0.54	-0.9
6,665,594	+		Pf6N2E2_5849	0.56	-1.3
6,665,594	+		Pf6N2E2_5849	0.56	-1.7
6,665,594	+		Pf6N2E2_5849	0.56	-0.6
6,665,595	-		Pf6N2E2_5849	0.56	-0.4
6,665,600	+		Pf6N2E2_5849	0.56	-1.8
6,665,600	+		Pf6N2E2_5849	0.56	-2.2
6,665,601	-		Pf6N2E2_5849	0.56	-2.9
6,665,837	+		Pf6N2E2_5849	0.70	-1.0
6,665,838	-		Pf6N2E2_5849	0.70	-0.8
6,666,032	+		Pf6N2E2_5849	0.82	-2.5
6,666,032	+		Pf6N2E2_5849	0.82	+0.2
6,666,362	+				-0.0
6,666,404	-				-0.3
6,666,527	+				+0.1
6,666,527	+				-0.1
6,666,528	-				+0.4
6,666,561	+				+1.5
6,666,561	+				+0.1
6,666,561	+				-0.6
6,666,561	+				+1.4
6,666,561	+				+0.6
6,666,561	+				-0.0
6,666,561	+				-0.1
6,666,561	+				-0.2
6,666,561	+				-0.2
6,666,561	+				-0.6
6,666,561	+				+0.4
6,666,562	-				+3.0
6,666,562	-				-0.3
6,666,562	-				-0.1
6,666,562	-				-0.9
6,666,562	-				+0.5
6,666,562	-				+0.0
6,666,563	+				-1.8
6,666,563	+				-0.9
6,666,563	+				-2.4
6,666,563	+				-0.2
6,666,563	+				+1.4
6,666,564	-				+0.4
6,666,564	-				+0.4
6,666,564	-				+0.1
6,666,564	-				+0.7
6,666,564	-				-0.5
6,666,564	-				-0.9
6,666,564	-				+0.2
6,666,564	-				+0.5
6,666,564	-				-0.7
6,666,565	+				-0.9
6,666,565	+				-0.7
6,666,565	+				-0.9
6,666,565	+				-0.4
6,666,565	+				-0.4
6,666,566	-				-2.2
6,666,566	-				+0.2
6,666,566	-				+0.2
6,666,566	-				+0.4
6,666,566	-				+0.3
6,666,566	-				-0.2
6,666,587	+				+1.4
6,666,588	-				+0.2
6,666,618	+				+1.4
6,666,618	+				-0.7
6,666,618	+				-0.4
6,666,618	+				-1.2
6,666,619	-				-0.5