Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5722 and Pf6N2E2_5723 are separated by 100 nucleotides Pf6N2E2_5723 and Pf6N2E2_5724 are separated by 113 nucleotides Pf6N2E2_5724 and Pf6N2E2_5725 are separated by 26 nucleotides
Pf6N2E2_5722 - 23S rRNA (Uracil-5-) -methyltransferase RumA (EC 2.1.1.-) ## LSU rRNA m(5)U1939, at 6,538,451 to 6,539,821
_5722
Pf6N2E2_5723 - GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2), at 6,539,922 to 6,542,168
_5723
Pf6N2E2_5724 - Nucleoside triphosphate pyrophosphohydrolase MazG (EC 3.6.1.8), at 6,542,282 to 6,543,115
_5724
Pf6N2E2_5725 - nucleoprotein/polynucleotide-associated enzyme, at 6,543,142 to 6,543,681
_5725
Position (kb)
6539
6540
6541
6542
6543 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6539.506 kb on + strand, within Pf6N2E2_5722 at 6539.532 kb on + strand, within Pf6N2E2_5722 at 6539.660 kb on + strand, within Pf6N2E2_5722 at 6539.660 kb on + strand, within Pf6N2E2_5722 at 6539.661 kb on - strand, within Pf6N2E2_5722 at 6539.661 kb on - strand, within Pf6N2E2_5722 at 6539.661 kb on - strand, within Pf6N2E2_5722 at 6539.661 kb on - strand, within Pf6N2E2_5722 at 6539.661 kb on - strand, within Pf6N2E2_5722 at 6539.661 kb on - strand, within Pf6N2E2_5722 at 6539.664 kb on + strand, within Pf6N2E2_5722 at 6539.664 kb on + strand, within Pf6N2E2_5722 at 6539.665 kb on - strand, within Pf6N2E2_5722 at 6539.749 kb on + strand at 6539.749 kb on + strand at 6539.788 kb on - strand at 6539.855 kb on + strand at 6539.904 kb on + strand at 6539.913 kb on + strand at 6539.913 kb on + strand at 6539.913 kb on + strand at 6539.913 kb on + strand at 6539.914 kb on - strand at 6539.917 kb on + strand at 6539.917 kb on + strand at 6539.966 kb on + strand at 6540.074 kb on + strand at 6540.422 kb on + strand, within Pf6N2E2_5723 at 6540.423 kb on - strand, within Pf6N2E2_5723 at 6540.423 kb on - strand, within Pf6N2E2_5723 at 6540.498 kb on + strand, within Pf6N2E2_5723 at 6540.498 kb on + strand, within Pf6N2E2_5723 at 6540.498 kb on + strand, within Pf6N2E2_5723 at 6540.498 kb on + strand, within Pf6N2E2_5723 at 6540.498 kb on + strand, within Pf6N2E2_5723 at 6540.498 kb on + strand, within Pf6N2E2_5723 at 6540.498 kb on + strand, within Pf6N2E2_5723 at 6540.498 kb on + strand, within Pf6N2E2_5723 at 6540.498 kb on + strand, within Pf6N2E2_5723 at 6540.498 kb on + strand, within Pf6N2E2_5723 at 6540.498 kb on + strand, within Pf6N2E2_5723 at 6540.498 kb on + strand, within Pf6N2E2_5723 at 6540.498 kb on + strand, within Pf6N2E2_5723 at 6540.499 kb on - strand, within Pf6N2E2_5723 at 6540.499 kb on - strand, within Pf6N2E2_5723 at 6540.499 kb on - strand, within Pf6N2E2_5723 at 6540.499 kb on - strand, within Pf6N2E2_5723 at 6540.499 kb on - strand, within Pf6N2E2_5723 at 6540.585 kb on + strand, within Pf6N2E2_5723 at 6540.586 kb on - strand, within Pf6N2E2_5723 at 6540.586 kb on - strand, within Pf6N2E2_5723 at 6540.586 kb on - strand, within Pf6N2E2_5723 at 6540.724 kb on - strand, within Pf6N2E2_5723 at 6540.845 kb on + strand, within Pf6N2E2_5723 at 6540.846 kb on - strand, within Pf6N2E2_5723 at 6540.850 kb on + strand, within Pf6N2E2_5723 at 6540.851 kb on - strand, within Pf6N2E2_5723 at 6540.851 kb on - strand, within Pf6N2E2_5723 at 6540.882 kb on + strand, within Pf6N2E2_5723 at 6540.883 kb on - strand, within Pf6N2E2_5723 at 6540.883 kb on - strand, within Pf6N2E2_5723 at 6540.952 kb on + strand, within Pf6N2E2_5723 at 6540.953 kb on - strand, within Pf6N2E2_5723 at 6540.953 kb on - strand, within Pf6N2E2_5723 at 6541.023 kb on + strand, within Pf6N2E2_5723 at 6541.023 kb on + strand, within Pf6N2E2_5723 at 6541.170 kb on + strand, within Pf6N2E2_5723 at 6541.170 kb on + strand, within Pf6N2E2_5723 at 6541.170 kb on + strand, within Pf6N2E2_5723 at 6541.171 kb on - strand, within Pf6N2E2_5723 at 6541.171 kb on - strand, within Pf6N2E2_5723 at 6541.171 kb on - strand, within Pf6N2E2_5723 at 6541.171 kb on - strand, within Pf6N2E2_5723 at 6541.172 kb on + strand, within Pf6N2E2_5723 at 6541.172 kb on + strand, within Pf6N2E2_5723 at 6541.172 kb on + strand, within Pf6N2E2_5723 at 6541.172 kb on + strand, within Pf6N2E2_5723 at 6541.173 kb on - strand, within Pf6N2E2_5723 at 6541.173 kb on - strand, within Pf6N2E2_5723 at 6541.173 kb on - strand, within Pf6N2E2_5723 at 6541.173 kb on - strand, within Pf6N2E2_5723 at 6541.173 kb on - strand, within Pf6N2E2_5723 at 6541.173 kb on - strand, within Pf6N2E2_5723 at 6541.173 kb on - strand, within Pf6N2E2_5723 at 6541.173 kb on - strand, within Pf6N2E2_5723 at 6541.308 kb on + strand, within Pf6N2E2_5723 at 6541.309 kb on - strand, within Pf6N2E2_5723 at 6541.309 kb on - strand, within Pf6N2E2_5723 at 6541.396 kb on - strand, within Pf6N2E2_5723 at 6541.696 kb on - strand, within Pf6N2E2_5723 at 6541.800 kb on + strand, within Pf6N2E2_5723 at 6541.800 kb on + strand, within Pf6N2E2_5723 at 6541.801 kb on - strand, within Pf6N2E2_5723 at 6541.801 kb on - strand, within Pf6N2E2_5723 at 6541.801 kb on - strand, within Pf6N2E2_5723 at 6541.923 kb on + strand, within Pf6N2E2_5723 at 6542.167 kb on - strand at 6542.167 kb on - strand at 6542.260 kb on + strand at 6542.261 kb on - strand at 6542.285 kb on + strand at 6542.285 kb on + strand at 6542.285 kb on + strand at 6542.285 kb on + strand at 6542.285 kb on + strand at 6542.286 kb on - strand at 6542.286 kb on - strand at 6542.286 kb on - strand at 6542.286 kb on - strand at 6542.286 kb on - strand at 6542.286 kb on - strand at 6542.286 kb on - strand at 6542.336 kb on + strand at 6542.366 kb on + strand, within Pf6N2E2_5724 at 6542.366 kb on + strand, within Pf6N2E2_5724 at 6542.386 kb on + strand, within Pf6N2E2_5724 at 6542.386 kb on + strand, within Pf6N2E2_5724 at 6542.386 kb on + strand, within Pf6N2E2_5724 at 6542.387 kb on - strand, within Pf6N2E2_5724 at 6542.387 kb on - strand, within Pf6N2E2_5724 at 6542.387 kb on - strand, within Pf6N2E2_5724 at 6542.387 kb on - strand, within Pf6N2E2_5724 at 6542.402 kb on + strand, within Pf6N2E2_5724 at 6542.402 kb on + strand, within Pf6N2E2_5724 at 6542.402 kb on + strand, within Pf6N2E2_5724 at 6542.403 kb on - strand, within Pf6N2E2_5724 at 6542.483 kb on + strand, within Pf6N2E2_5724 at 6542.483 kb on + strand, within Pf6N2E2_5724 at 6542.483 kb on + strand, within Pf6N2E2_5724 at 6542.483 kb on + strand, within Pf6N2E2_5724 at 6542.483 kb on + strand, within Pf6N2E2_5724 at 6542.484 kb on - strand, within Pf6N2E2_5724 at 6542.484 kb on - strand, within Pf6N2E2_5724 at 6542.484 kb on - strand, within Pf6N2E2_5724 at 6542.486 kb on + strand, within Pf6N2E2_5724 at 6542.486 kb on + strand, within Pf6N2E2_5724 at 6542.486 kb on + strand, within Pf6N2E2_5724 at 6542.486 kb on + strand, within Pf6N2E2_5724 at 6542.487 kb on - strand, within Pf6N2E2_5724 at 6542.487 kb on - strand, within Pf6N2E2_5724 at 6542.487 kb on - strand, within Pf6N2E2_5724 at 6542.487 kb on - strand, within Pf6N2E2_5724 at 6542.487 kb on - strand, within Pf6N2E2_5724 at 6542.487 kb on - strand, within Pf6N2E2_5724 at 6542.487 kb on - strand, within Pf6N2E2_5724 at 6542.598 kb on - strand, within Pf6N2E2_5724 at 6542.850 kb on - strand, within Pf6N2E2_5724 at 6542.999 kb on - strand, within Pf6N2E2_5724 at 6543.126 kb on + strand at 6543.126 kb on + strand at 6543.127 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 6,539,506 + Pf6N2E2_5722 0.77 -1.4 6,539,532 + Pf6N2E2_5722 0.79 -1.7 6,539,660 + Pf6N2E2_5722 0.88 -0.6 6,539,660 + Pf6N2E2_5722 0.88 -1.1 6,539,661 - Pf6N2E2_5722 0.88 +1.4 6,539,661 - Pf6N2E2_5722 0.88 +1.4 6,539,661 - Pf6N2E2_5722 0.88 +0.9 6,539,661 - Pf6N2E2_5722 0.88 +1.4 6,539,661 - Pf6N2E2_5722 0.88 +1.4 6,539,661 - Pf6N2E2_5722 0.88 +1.4 6,539,664 + Pf6N2E2_5722 0.88 -0.2 6,539,664 + Pf6N2E2_5722 0.88 -0.3 6,539,665 - Pf6N2E2_5722 0.89 +1.4 6,539,749 + -0.7 6,539,749 + +0.4 6,539,788 - -0.2 6,539,855 + -1.6 6,539,904 + -1.0 6,539,913 + -0.2 6,539,913 + -0.9 6,539,913 + +0.1 6,539,913 + +0.4 6,539,914 - -0.3 6,539,917 + -0.7 6,539,917 + +0.3 6,539,966 + -0.2 6,540,074 + -0.4 6,540,422 + Pf6N2E2_5723 0.22 +1.4 6,540,423 - Pf6N2E2_5723 0.22 +1.4 6,540,423 - Pf6N2E2_5723 0.22 -2.8 6,540,498 + Pf6N2E2_5723 0.26 -1.7 6,540,498 + Pf6N2E2_5723 0.26 -2.1 6,540,498 + Pf6N2E2_5723 0.26 -3.0 6,540,498 + Pf6N2E2_5723 0.26 +0.4 6,540,498 + Pf6N2E2_5723 0.26 +0.4 6,540,498 + Pf6N2E2_5723 0.26 +1.1 6,540,498 + Pf6N2E2_5723 0.26 +0.4 6,540,498 + Pf6N2E2_5723 0.26 -1.6 6,540,498 + Pf6N2E2_5723 0.26 -1.2 6,540,498 + Pf6N2E2_5723 0.26 +1.4 6,540,498 + Pf6N2E2_5723 0.26 -0.6 6,540,498 + Pf6N2E2_5723 0.26 -0.9 6,540,498 + Pf6N2E2_5723 0.26 -0.6 6,540,499 - Pf6N2E2_5723 0.26 +1.4 6,540,499 - Pf6N2E2_5723 0.26 -0.6 6,540,499 - Pf6N2E2_5723 0.26 +1.4 6,540,499 - Pf6N2E2_5723 0.26 +1.4 6,540,499 - Pf6N2E2_5723 0.26 +1.4 6,540,585 + Pf6N2E2_5723 0.30 -1.1 6,540,586 - Pf6N2E2_5723 0.30 -1.7 6,540,586 - Pf6N2E2_5723 0.30 +2.4 6,540,586 - Pf6N2E2_5723 0.30 +0.4 6,540,724 - Pf6N2E2_5723 0.36 +0.4 6,540,845 + Pf6N2E2_5723 0.41 +1.4 6,540,846 - Pf6N2E2_5723 0.41 +1.4 6,540,850 + Pf6N2E2_5723 0.41 +1.4 6,540,851 - Pf6N2E2_5723 0.41 -1.1 6,540,851 - Pf6N2E2_5723 0.41 +0.4 6,540,882 + Pf6N2E2_5723 0.43 -0.7 6,540,883 - Pf6N2E2_5723 0.43 +1.4 6,540,883 - Pf6N2E2_5723 0.43 -0.6 6,540,952 + Pf6N2E2_5723 0.46 -0.1 6,540,953 - Pf6N2E2_5723 0.46 +1.4 6,540,953 - Pf6N2E2_5723 0.46 +0.2 6,541,023 + Pf6N2E2_5723 0.49 +1.4 6,541,023 + Pf6N2E2_5723 0.49 -1.0 6,541,170 + Pf6N2E2_5723 0.56 -2.7 6,541,170 + Pf6N2E2_5723 0.56 +0.4 6,541,170 + Pf6N2E2_5723 0.56 +1.4 6,541,171 - Pf6N2E2_5723 0.56 -2.0 6,541,171 - Pf6N2E2_5723 0.56 -0.7 6,541,171 - Pf6N2E2_5723 0.56 -1.7 6,541,171 - Pf6N2E2_5723 0.56 +0.4 6,541,172 + Pf6N2E2_5723 0.56 -0.7 6,541,172 + Pf6N2E2_5723 0.56 -0.2 6,541,172 + Pf6N2E2_5723 0.56 -1.4 6,541,172 + Pf6N2E2_5723 0.56 -0.6 6,541,173 - Pf6N2E2_5723 0.56 +1.4 6,541,173 - Pf6N2E2_5723 0.56 -2.3 6,541,173 - Pf6N2E2_5723 0.56 +0.4 6,541,173 - Pf6N2E2_5723 0.56 -1.4 6,541,173 - Pf6N2E2_5723 0.56 +1.4 6,541,173 - Pf6N2E2_5723 0.56 +1.4 6,541,173 - Pf6N2E2_5723 0.56 +1.4 6,541,173 - Pf6N2E2_5723 0.56 -0.6 6,541,308 + Pf6N2E2_5723 0.62 -0.6 6,541,309 - Pf6N2E2_5723 0.62 +1.4 6,541,309 - Pf6N2E2_5723 0.62 -0.8 6,541,396 - Pf6N2E2_5723 0.66 -0.4 6,541,696 - Pf6N2E2_5723 0.79 -2.1 6,541,800 + Pf6N2E2_5723 0.84 -1.8 6,541,800 + Pf6N2E2_5723 0.84 -0.7 6,541,801 - Pf6N2E2_5723 0.84 -0.9 6,541,801 - Pf6N2E2_5723 0.84 -2.2 6,541,801 - Pf6N2E2_5723 0.84 -0.9 6,541,923 + Pf6N2E2_5723 0.89 -0.2 6,542,167 - -0.8 6,542,167 - -0.1 6,542,260 + -1.4 6,542,261 - -0.4 6,542,285 + -0.1 6,542,285 + +0.1 6,542,285 + +1.4 6,542,285 + +1.0 6,542,285 + +1.4 6,542,286 - +0.3 6,542,286 - +0.6 6,542,286 - -1.3 6,542,286 - +0.2 6,542,286 - +0.0 6,542,286 - -0.0 6,542,286 - +1.2 6,542,336 + -0.4 6,542,366 + Pf6N2E2_5724 0.10 +0.8 6,542,366 + Pf6N2E2_5724 0.10 +1.4 6,542,386 + Pf6N2E2_5724 0.12 -0.3 6,542,386 + Pf6N2E2_5724 0.12 +0.1 6,542,386 + Pf6N2E2_5724 0.12 +1.6 6,542,387 - Pf6N2E2_5724 0.13 +0.6 6,542,387 - Pf6N2E2_5724 0.13 +0.1 6,542,387 - Pf6N2E2_5724 0.13 -0.3 6,542,387 - Pf6N2E2_5724 0.13 -0.6 6,542,402 + Pf6N2E2_5724 0.14 -0.2 6,542,402 + Pf6N2E2_5724 0.14 +0.1 6,542,402 + Pf6N2E2_5724 0.14 -0.5 6,542,403 - Pf6N2E2_5724 0.15 +0.3 6,542,483 + Pf6N2E2_5724 0.24 -0.2 6,542,483 + Pf6N2E2_5724 0.24 +0.4 6,542,483 + Pf6N2E2_5724 0.24 +0.2 6,542,483 + Pf6N2E2_5724 0.24 -0.3 6,542,483 + Pf6N2E2_5724 0.24 -1.8 6,542,484 - Pf6N2E2_5724 0.24 -0.3 6,542,484 - Pf6N2E2_5724 0.24 +0.4 6,542,484 - Pf6N2E2_5724 0.24 -0.9 6,542,486 + Pf6N2E2_5724 0.24 -0.3 6,542,486 + Pf6N2E2_5724 0.24 +1.6 6,542,486 + Pf6N2E2_5724 0.24 +0.7 6,542,486 + Pf6N2E2_5724 0.24 -0.6 6,542,487 - Pf6N2E2_5724 0.25 +0.3 6,542,487 - Pf6N2E2_5724 0.25 +0.5 6,542,487 - Pf6N2E2_5724 0.25 -0.6 6,542,487 - Pf6N2E2_5724 0.25 -1.3 6,542,487 - Pf6N2E2_5724 0.25 -0.4 6,542,487 - Pf6N2E2_5724 0.25 +0.3 6,542,487 - Pf6N2E2_5724 0.25 -1.0 6,542,598 - Pf6N2E2_5724 0.38 +1.4 6,542,850 - Pf6N2E2_5724 0.68 -0.1 6,542,999 - Pf6N2E2_5724 0.86 +1.2 6,543,126 + +0.1 6,543,126 + +1.0 6,543,127 - +0.2
Or see this region's nucleotide sequence