Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5705

Experiment: Nitrate reduction, replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_5703 and Pf6N2E2_5704 are separated by 104 nucleotidesPf6N2E2_5704 and Pf6N2E2_5705 overlap by 4 nucleotidesPf6N2E2_5705 and Pf6N2E2_5706 are separated by 163 nucleotides Pf6N2E2_5703 - outer membrane protein H1, at 6,519,011 to 6,519,610 _5703 Pf6N2E2_5704 - Transcriptional regulatory protein PhoP, at 6,519,715 to 6,520,392 _5704 Pf6N2E2_5705 - Sensor protein PhoQ (EC 2.7.13.3), at 6,520,389 to 6,521,735 _5705 Pf6N2E2_5706 - Transcriptional regulator, AraC family, at 6,521,899 to 6,522,897 _5706 Position (kb) 6520 6521 6522Strain fitness (log2 ratio) -2 -1 0 1at 6519.491 kb on + strandat 6519.584 kb on + strandat 6519.584 kb on + strandat 6519.584 kb on + strandat 6519.600 kb on - strandat 6519.629 kb on - strandat 6520.006 kb on + strand, within Pf6N2E2_5704at 6520.006 kb on + strand, within Pf6N2E2_5704at 6520.006 kb on + strand, within Pf6N2E2_5704at 6520.006 kb on + strand, within Pf6N2E2_5704at 6520.007 kb on - strand, within Pf6N2E2_5704at 6520.007 kb on - strand, within Pf6N2E2_5704at 6520.007 kb on - strand, within Pf6N2E2_5704at 6520.081 kb on - strand, within Pf6N2E2_5704at 6520.172 kb on - strand, within Pf6N2E2_5704at 6520.177 kb on + strand, within Pf6N2E2_5704at 6520.193 kb on - strand, within Pf6N2E2_5704at 6520.636 kb on - strand, within Pf6N2E2_5705at 6520.641 kb on + strand, within Pf6N2E2_5705at 6520.701 kb on + strand, within Pf6N2E2_5705at 6520.776 kb on + strand, within Pf6N2E2_5705at 6520.830 kb on - strand, within Pf6N2E2_5705at 6521.661 kb on + strandat 6521.742 kb on + strandat 6522.035 kb on + strand, within Pf6N2E2_5706at 6522.035 kb on + strand, within Pf6N2E2_5706at 6522.035 kb on + strand, within Pf6N2E2_5706at 6522.035 kb on + strand, within Pf6N2E2_5706at 6522.036 kb on - strand, within Pf6N2E2_5706at 6522.211 kb on - strand, within Pf6N2E2_5706at 6522.469 kb on + strand, within Pf6N2E2_5706at 6522.590 kb on + strand, within Pf6N2E2_5706at 6522.591 kb on - strand, within Pf6N2E2_5706at 6522.591 kb on - strand, within Pf6N2E2_5706at 6522.676 kb on - strand, within Pf6N2E2_5706at 6522.701 kb on + strand, within Pf6N2E2_5706at 6522.701 kb on + strand, within Pf6N2E2_5706at 6522.702 kb on - strand, within Pf6N2E2_5706

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A
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6,519,491 + +0.4
6,519,584 + +0.6
6,519,584 + -0.2
6,519,584 + +1.4
6,519,600 - +0.2
6,519,629 - +1.4
6,520,006 + Pf6N2E2_5704 0.43 +1.4
6,520,006 + Pf6N2E2_5704 0.43 +0.2
6,520,006 + Pf6N2E2_5704 0.43 -0.2
6,520,006 + Pf6N2E2_5704 0.43 +0.4
6,520,007 - Pf6N2E2_5704 0.43 +0.4
6,520,007 - Pf6N2E2_5704 0.43 +1.4
6,520,007 - Pf6N2E2_5704 0.43 +0.3
6,520,081 - Pf6N2E2_5704 0.54 -0.3
6,520,172 - Pf6N2E2_5704 0.67 +1.4
6,520,177 + Pf6N2E2_5704 0.68 -0.3
6,520,193 - Pf6N2E2_5704 0.71 +1.4
6,520,636 - Pf6N2E2_5705 0.18 +0.1
6,520,641 + Pf6N2E2_5705 0.19 -1.5
6,520,701 + Pf6N2E2_5705 0.23 -0.6
6,520,776 + Pf6N2E2_5705 0.29 -1.5
6,520,830 - Pf6N2E2_5705 0.33 +0.4
6,521,661 + +0.4
6,521,742 + -1.1
6,522,035 + Pf6N2E2_5706 0.14 +0.3
6,522,035 + Pf6N2E2_5706 0.14 +1.0
6,522,035 + Pf6N2E2_5706 0.14 -0.1
6,522,035 + Pf6N2E2_5706 0.14 -0.0
6,522,036 - Pf6N2E2_5706 0.14 -1.7
6,522,211 - Pf6N2E2_5706 0.31 +1.1
6,522,469 + Pf6N2E2_5706 0.57 -2.4
6,522,590 + Pf6N2E2_5706 0.69 -0.6
6,522,591 - Pf6N2E2_5706 0.69 +1.4
6,522,591 - Pf6N2E2_5706 0.69 -0.0
6,522,676 - Pf6N2E2_5706 0.78 +1.3
6,522,701 + Pf6N2E2_5706 0.80 +1.4
6,522,701 + Pf6N2E2_5706 0.80 +1.4
6,522,702 - Pf6N2E2_5706 0.80 +1.4

Or see this region's nucleotide sequence