Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5703 and Pf6N2E2_5704 are separated by 104 nucleotides Pf6N2E2_5704 and Pf6N2E2_5705 overlap by 4 nucleotides Pf6N2E2_5705 and Pf6N2E2_5706 are separated by 163 nucleotides
Pf6N2E2_5703 - outer membrane protein H1, at 6,519,011 to 6,519,610
_5703
Pf6N2E2_5704 - Transcriptional regulatory protein PhoP, at 6,519,715 to 6,520,392
_5704
Pf6N2E2_5705 - Sensor protein PhoQ (EC 2.7.13.3), at 6,520,389 to 6,521,735
_5705
Pf6N2E2_5706 - Transcriptional regulator, AraC family, at 6,521,899 to 6,522,897
_5706
Position (kb)
6520
6521
6522 Strain fitness (log2 ratio)
-2
-1
0
1 at 6519.491 kb on + strand at 6519.584 kb on + strand at 6519.584 kb on + strand at 6519.584 kb on + strand at 6519.600 kb on - strand at 6519.629 kb on - strand at 6520.006 kb on + strand, within Pf6N2E2_5704 at 6520.006 kb on + strand, within Pf6N2E2_5704 at 6520.006 kb on + strand, within Pf6N2E2_5704 at 6520.006 kb on + strand, within Pf6N2E2_5704 at 6520.007 kb on - strand, within Pf6N2E2_5704 at 6520.007 kb on - strand, within Pf6N2E2_5704 at 6520.007 kb on - strand, within Pf6N2E2_5704 at 6520.081 kb on - strand, within Pf6N2E2_5704 at 6520.172 kb on - strand, within Pf6N2E2_5704 at 6520.177 kb on + strand, within Pf6N2E2_5704 at 6520.193 kb on - strand, within Pf6N2E2_5704 at 6520.636 kb on - strand, within Pf6N2E2_5705 at 6520.641 kb on + strand, within Pf6N2E2_5705 at 6520.701 kb on + strand, within Pf6N2E2_5705 at 6520.776 kb on + strand, within Pf6N2E2_5705 at 6520.830 kb on - strand, within Pf6N2E2_5705 at 6521.661 kb on + strand at 6521.742 kb on + strand at 6522.035 kb on + strand, within Pf6N2E2_5706 at 6522.035 kb on + strand, within Pf6N2E2_5706 at 6522.035 kb on + strand, within Pf6N2E2_5706 at 6522.035 kb on + strand, within Pf6N2E2_5706 at 6522.036 kb on - strand, within Pf6N2E2_5706 at 6522.211 kb on - strand, within Pf6N2E2_5706 at 6522.469 kb on + strand, within Pf6N2E2_5706 at 6522.590 kb on + strand, within Pf6N2E2_5706 at 6522.591 kb on - strand, within Pf6N2E2_5706 at 6522.591 kb on - strand, within Pf6N2E2_5706 at 6522.676 kb on - strand, within Pf6N2E2_5706 at 6522.701 kb on + strand, within Pf6N2E2_5706 at 6522.701 kb on + strand, within Pf6N2E2_5706 at 6522.702 kb on - strand, within Pf6N2E2_5706
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 6,519,491 + +0.4 6,519,584 + +0.6 6,519,584 + -0.2 6,519,584 + +1.4 6,519,600 - +0.2 6,519,629 - +1.4 6,520,006 + Pf6N2E2_5704 0.43 +1.4 6,520,006 + Pf6N2E2_5704 0.43 +0.2 6,520,006 + Pf6N2E2_5704 0.43 -0.2 6,520,006 + Pf6N2E2_5704 0.43 +0.4 6,520,007 - Pf6N2E2_5704 0.43 +0.4 6,520,007 - Pf6N2E2_5704 0.43 +1.4 6,520,007 - Pf6N2E2_5704 0.43 +0.3 6,520,081 - Pf6N2E2_5704 0.54 -0.3 6,520,172 - Pf6N2E2_5704 0.67 +1.4 6,520,177 + Pf6N2E2_5704 0.68 -0.3 6,520,193 - Pf6N2E2_5704 0.71 +1.4 6,520,636 - Pf6N2E2_5705 0.18 +0.1 6,520,641 + Pf6N2E2_5705 0.19 -1.5 6,520,701 + Pf6N2E2_5705 0.23 -0.6 6,520,776 + Pf6N2E2_5705 0.29 -1.5 6,520,830 - Pf6N2E2_5705 0.33 +0.4 6,521,661 + +0.4 6,521,742 + -1.1 6,522,035 + Pf6N2E2_5706 0.14 +0.3 6,522,035 + Pf6N2E2_5706 0.14 +1.0 6,522,035 + Pf6N2E2_5706 0.14 -0.1 6,522,035 + Pf6N2E2_5706 0.14 -0.0 6,522,036 - Pf6N2E2_5706 0.14 -1.7 6,522,211 - Pf6N2E2_5706 0.31 +1.1 6,522,469 + Pf6N2E2_5706 0.57 -2.4 6,522,590 + Pf6N2E2_5706 0.69 -0.6 6,522,591 - Pf6N2E2_5706 0.69 +1.4 6,522,591 - Pf6N2E2_5706 0.69 -0.0 6,522,676 - Pf6N2E2_5706 0.78 +1.3 6,522,701 + Pf6N2E2_5706 0.80 +1.4 6,522,701 + Pf6N2E2_5706 0.80 +1.4 6,522,702 - Pf6N2E2_5706 0.80 +1.4
Or see this region's nucleotide sequence