Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5565 and Pf6N2E2_5566 overlap by 4 nucleotides Pf6N2E2_5566 and Pf6N2E2_5567 are separated by 212 nucleotides Pf6N2E2_5567 and Pf6N2E2_5568 overlap by 4 nucleotides
Pf6N2E2_5565 - Two component, Sigma-54 Specific, central transcriptional regulator of acidic amino acid uptake, at 6,382,692 to 6,384,020
_5565
Pf6N2E2_5566 - Signal transduction histidine kinase regulating C4-dicarboxylate transport system (EC 2.7.13.3), at 6,384,017 to 6,385,921
_5566
Pf6N2E2_5567 - Glutamate Aspartate transport ATP-binding protein GltL (TC 3.A.1.3.4), at 6,386,134 to 6,386,868
_5567
Pf6N2E2_5568 - Glutamate Aspartate transport system permease protein GltK (TC 3.A.1.3.4), at 6,386,865 to 6,387,536
_5568
Position (kb)
6384
6385
6386 Strain fitness (log2 ratio)
-1
0
1
2 at 6383.110 kb on + strand, within Pf6N2E2_5565 at 6383.110 kb on + strand, within Pf6N2E2_5565 at 6383.110 kb on + strand, within Pf6N2E2_5565 at 6383.110 kb on + strand, within Pf6N2E2_5565 at 6383.110 kb on + strand, within Pf6N2E2_5565 at 6383.110 kb on + strand, within Pf6N2E2_5565 at 6383.110 kb on + strand, within Pf6N2E2_5565 at 6383.110 kb on + strand, within Pf6N2E2_5565 at 6383.110 kb on + strand, within Pf6N2E2_5565 at 6383.111 kb on - strand, within Pf6N2E2_5565 at 6383.111 kb on - strand, within Pf6N2E2_5565 at 6383.111 kb on - strand, within Pf6N2E2_5565 at 6383.111 kb on - strand, within Pf6N2E2_5565 at 6383.221 kb on - strand, within Pf6N2E2_5565 at 6383.221 kb on - strand, within Pf6N2E2_5565 at 6383.352 kb on - strand, within Pf6N2E2_5565 at 6383.722 kb on + strand, within Pf6N2E2_5565 at 6383.722 kb on + strand, within Pf6N2E2_5565 at 6383.722 kb on + strand, within Pf6N2E2_5565 at 6383.722 kb on + strand, within Pf6N2E2_5565 at 6383.723 kb on - strand, within Pf6N2E2_5565 at 6383.723 kb on - strand, within Pf6N2E2_5565 at 6383.882 kb on + strand, within Pf6N2E2_5565 at 6384.000 kb on + strand at 6384.000 kb on + strand at 6384.000 kb on + strand at 6384.000 kb on + strand at 6384.000 kb on + strand at 6384.001 kb on - strand at 6384.001 kb on - strand at 6384.035 kb on + strand at 6384.044 kb on + strand at 6384.045 kb on - strand at 6384.054 kb on - strand at 6384.259 kb on - strand, within Pf6N2E2_5566 at 6384.259 kb on - strand, within Pf6N2E2_5566 at 6385.024 kb on + strand, within Pf6N2E2_5566 at 6385.024 kb on + strand, within Pf6N2E2_5566 at 6385.025 kb on - strand, within Pf6N2E2_5566 at 6385.025 kb on - strand, within Pf6N2E2_5566 at 6385.053 kb on + strand, within Pf6N2E2_5566 at 6385.053 kb on + strand, within Pf6N2E2_5566 at 6385.053 kb on + strand, within Pf6N2E2_5566 at 6385.054 kb on - strand, within Pf6N2E2_5566 at 6385.054 kb on - strand, within Pf6N2E2_5566 at 6385.054 kb on - strand, within Pf6N2E2_5566 at 6385.054 kb on - strand, within Pf6N2E2_5566 at 6385.170 kb on + strand, within Pf6N2E2_5566 at 6385.171 kb on - strand, within Pf6N2E2_5566 at 6385.173 kb on + strand, within Pf6N2E2_5566 at 6385.173 kb on + strand, within Pf6N2E2_5566 at 6385.174 kb on - strand, within Pf6N2E2_5566 at 6385.174 kb on - strand, within Pf6N2E2_5566 at 6385.174 kb on - strand, within Pf6N2E2_5566 at 6385.261 kb on + strand, within Pf6N2E2_5566 at 6385.395 kb on + strand, within Pf6N2E2_5566 at 6385.396 kb on - strand, within Pf6N2E2_5566 at 6385.396 kb on - strand, within Pf6N2E2_5566 at 6385.396 kb on - strand, within Pf6N2E2_5566 at 6385.396 kb on - strand, within Pf6N2E2_5566 at 6385.398 kb on + strand, within Pf6N2E2_5566 at 6385.398 kb on + strand, within Pf6N2E2_5566 at 6385.418 kb on - strand, within Pf6N2E2_5566 at 6385.431 kb on + strand, within Pf6N2E2_5566 at 6385.470 kb on + strand, within Pf6N2E2_5566 at 6385.501 kb on - strand, within Pf6N2E2_5566 at 6385.501 kb on - strand, within Pf6N2E2_5566 at 6385.501 kb on - strand, within Pf6N2E2_5566 at 6385.501 kb on - strand, within Pf6N2E2_5566 at 6385.501 kb on - strand, within Pf6N2E2_5566 at 6385.560 kb on + strand, within Pf6N2E2_5566 at 6385.560 kb on + strand, within Pf6N2E2_5566 at 6385.560 kb on + strand, within Pf6N2E2_5566 at 6385.560 kb on + strand, within Pf6N2E2_5566 at 6385.560 kb on + strand, within Pf6N2E2_5566 at 6385.561 kb on - strand, within Pf6N2E2_5566 at 6385.561 kb on - strand, within Pf6N2E2_5566 at 6385.561 kb on - strand, within Pf6N2E2_5566 at 6385.602 kb on + strand, within Pf6N2E2_5566 at 6385.689 kb on + strand, within Pf6N2E2_5566 at 6385.689 kb on + strand, within Pf6N2E2_5566 at 6385.689 kb on + strand, within Pf6N2E2_5566 at 6385.689 kb on + strand, within Pf6N2E2_5566 at 6385.689 kb on + strand, within Pf6N2E2_5566 at 6385.690 kb on - strand, within Pf6N2E2_5566 at 6385.690 kb on - strand, within Pf6N2E2_5566 at 6385.695 kb on + strand, within Pf6N2E2_5566 at 6385.695 kb on + strand, within Pf6N2E2_5566 at 6385.695 kb on + strand, within Pf6N2E2_5566 at 6385.695 kb on + strand, within Pf6N2E2_5566 at 6385.695 kb on + strand, within Pf6N2E2_5566 at 6385.695 kb on + strand, within Pf6N2E2_5566 at 6385.695 kb on + strand, within Pf6N2E2_5566 at 6385.695 kb on + strand, within Pf6N2E2_5566 at 6385.695 kb on + strand, within Pf6N2E2_5566 at 6385.695 kb on + strand, within Pf6N2E2_5566 at 6385.695 kb on + strand, within Pf6N2E2_5566 at 6385.696 kb on - strand, within Pf6N2E2_5566 at 6385.696 kb on - strand, within Pf6N2E2_5566 at 6385.696 kb on - strand, within Pf6N2E2_5566 at 6385.696 kb on - strand, within Pf6N2E2_5566 at 6385.769 kb on + strand at 6385.769 kb on + strand at 6385.769 kb on + strand at 6385.769 kb on + strand at 6385.770 kb on - strand at 6385.776 kb on + strand at 6385.777 kb on - strand at 6385.777 kb on - strand at 6386.020 kb on + strand at 6386.037 kb on - strand at 6386.045 kb on + strand at 6386.046 kb on - strand at 6386.136 kb on - strand at 6386.136 kb on - strand at 6386.248 kb on + strand, within Pf6N2E2_5567 at 6386.248 kb on + strand, within Pf6N2E2_5567 at 6386.249 kb on - strand, within Pf6N2E2_5567 at 6386.821 kb on + strand at 6386.838 kb on - strand at 6386.859 kb on + strand at 6386.859 kb on + strand at 6386.868 kb on + strand at 6386.868 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 6,383,110 + Pf6N2E2_5565 0.31 +0.6 6,383,110 + Pf6N2E2_5565 0.31 -0.8 6,383,110 + Pf6N2E2_5565 0.31 +0.3 6,383,110 + Pf6N2E2_5565 0.31 -1.6 6,383,110 + Pf6N2E2_5565 0.31 +0.5 6,383,110 + Pf6N2E2_5565 0.31 +0.4 6,383,110 + Pf6N2E2_5565 0.31 -0.1 6,383,110 + Pf6N2E2_5565 0.31 -0.6 6,383,110 + Pf6N2E2_5565 0.31 -0.0 6,383,111 - Pf6N2E2_5565 0.32 +1.1 6,383,111 - Pf6N2E2_5565 0.32 +0.4 6,383,111 - Pf6N2E2_5565 0.32 +0.5 6,383,111 - Pf6N2E2_5565 0.32 +2.0 6,383,221 - Pf6N2E2_5565 0.40 -0.1 6,383,221 - Pf6N2E2_5565 0.40 +0.7 6,383,352 - Pf6N2E2_5565 0.50 +0.7 6,383,722 + Pf6N2E2_5565 0.78 -0.9 6,383,722 + Pf6N2E2_5565 0.78 +0.0 6,383,722 + Pf6N2E2_5565 0.78 +0.1 6,383,722 + Pf6N2E2_5565 0.78 -1.7 6,383,723 - Pf6N2E2_5565 0.78 +1.0 6,383,723 - Pf6N2E2_5565 0.78 +0.2 6,383,882 + Pf6N2E2_5565 0.90 +0.1 6,384,000 + +0.0 6,384,000 + -0.3 6,384,000 + +0.4 6,384,000 + +0.4 6,384,000 + +0.4 6,384,001 - -0.1 6,384,001 - -1.4 6,384,035 + -0.3 6,384,044 + +0.6 6,384,045 - -0.7 6,384,054 - -0.2 6,384,259 - Pf6N2E2_5566 0.13 -0.5 6,384,259 - Pf6N2E2_5566 0.13 +0.5 6,385,024 + Pf6N2E2_5566 0.53 +1.1 6,385,024 + Pf6N2E2_5566 0.53 +1.1 6,385,025 - Pf6N2E2_5566 0.53 +0.7 6,385,025 - Pf6N2E2_5566 0.53 +2.1 6,385,053 + Pf6N2E2_5566 0.54 +0.7 6,385,053 + Pf6N2E2_5566 0.54 -0.4 6,385,053 + Pf6N2E2_5566 0.54 +0.4 6,385,054 - Pf6N2E2_5566 0.54 +0.4 6,385,054 - Pf6N2E2_5566 0.54 +1.4 6,385,054 - Pf6N2E2_5566 0.54 -0.9 6,385,054 - Pf6N2E2_5566 0.54 +0.4 6,385,170 + Pf6N2E2_5566 0.61 +1.0 6,385,171 - Pf6N2E2_5566 0.61 -0.0 6,385,173 + Pf6N2E2_5566 0.61 -0.6 6,385,173 + Pf6N2E2_5566 0.61 +0.6 6,385,174 - Pf6N2E2_5566 0.61 +0.5 6,385,174 - Pf6N2E2_5566 0.61 -1.2 6,385,174 - Pf6N2E2_5566 0.61 +0.3 6,385,261 + Pf6N2E2_5566 0.65 +0.4 6,385,395 + Pf6N2E2_5566 0.72 +0.2 6,385,396 - Pf6N2E2_5566 0.72 +0.5 6,385,396 - Pf6N2E2_5566 0.72 -0.0 6,385,396 - Pf6N2E2_5566 0.72 +0.5 6,385,396 - Pf6N2E2_5566 0.72 -1.3 6,385,398 + Pf6N2E2_5566 0.72 -0.3 6,385,398 + Pf6N2E2_5566 0.72 +1.0 6,385,418 - Pf6N2E2_5566 0.74 -0.1 6,385,431 + Pf6N2E2_5566 0.74 +0.5 6,385,470 + Pf6N2E2_5566 0.76 -0.2 6,385,501 - Pf6N2E2_5566 0.78 +0.4 6,385,501 - Pf6N2E2_5566 0.78 +0.5 6,385,501 - Pf6N2E2_5566 0.78 +0.0 6,385,501 - Pf6N2E2_5566 0.78 +0.3 6,385,501 - Pf6N2E2_5566 0.78 +1.0 6,385,560 + Pf6N2E2_5566 0.81 +0.6 6,385,560 + Pf6N2E2_5566 0.81 -0.0 6,385,560 + Pf6N2E2_5566 0.81 -0.6 6,385,560 + Pf6N2E2_5566 0.81 -0.1 6,385,560 + Pf6N2E2_5566 0.81 +0.4 6,385,561 - Pf6N2E2_5566 0.81 +0.3 6,385,561 - Pf6N2E2_5566 0.81 +0.3 6,385,561 - Pf6N2E2_5566 0.81 -0.5 6,385,602 + Pf6N2E2_5566 0.83 +0.1 6,385,689 + Pf6N2E2_5566 0.88 +1.4 6,385,689 + Pf6N2E2_5566 0.88 +0.1 6,385,689 + Pf6N2E2_5566 0.88 +0.7 6,385,689 + Pf6N2E2_5566 0.88 -0.7 6,385,689 + Pf6N2E2_5566 0.88 +0.0 6,385,690 - Pf6N2E2_5566 0.88 -0.0 6,385,690 - Pf6N2E2_5566 0.88 +0.4 6,385,695 + Pf6N2E2_5566 0.88 -0.6 6,385,695 + Pf6N2E2_5566 0.88 +0.5 6,385,695 + Pf6N2E2_5566 0.88 +0.5 6,385,695 + Pf6N2E2_5566 0.88 +1.2 6,385,695 + Pf6N2E2_5566 0.88 -0.5 6,385,695 + Pf6N2E2_5566 0.88 -0.2 6,385,695 + Pf6N2E2_5566 0.88 -1.2 6,385,695 + Pf6N2E2_5566 0.88 +0.3 6,385,695 + Pf6N2E2_5566 0.88 +0.2 6,385,695 + Pf6N2E2_5566 0.88 +0.0 6,385,695 + Pf6N2E2_5566 0.88 +1.0 6,385,696 - Pf6N2E2_5566 0.88 +1.0 6,385,696 - Pf6N2E2_5566 0.88 -0.6 6,385,696 - Pf6N2E2_5566 0.88 +0.8 6,385,696 - Pf6N2E2_5566 0.88 +0.5 6,385,769 + +0.7 6,385,769 + -1.0 6,385,769 + +0.3 6,385,769 + +1.4 6,385,770 - +0.1 6,385,776 + +0.4 6,385,777 - +0.2 6,385,777 - +0.2 6,386,020 + +1.4 6,386,037 - +0.4 6,386,045 + +0.4 6,386,046 - +0.6 6,386,136 - -0.8 6,386,136 - +0.0 6,386,248 + Pf6N2E2_5567 0.16 -0.3 6,386,248 + Pf6N2E2_5567 0.16 -1.4 6,386,249 - Pf6N2E2_5567 0.16 -0.0 6,386,821 + +1.8 6,386,838 - -1.3 6,386,859 + +1.4 6,386,859 + +0.2 6,386,868 + -0.4 6,386,868 + +0.1
Or see this region's nucleotide sequence