Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5518 and Pf6N2E2_5519 are separated by 135 nucleotides Pf6N2E2_5519 and Pf6N2E2_5520 are separated by 2 nucleotides Pf6N2E2_5520 and Pf6N2E2_5521 are separated by 46 nucleotides
Pf6N2E2_5518 - Mutator mutT protein (7,8-dihydro-8-oxoguanine-triphosphatase) (EC 3.6.1.-) / Thiamin-phosphate pyrophosphorylase-like protein, at 6,337,187 to 6,338,116
_5518
Pf6N2E2_5519 - ATP:Cob(I)alamin adenosyltransferase (EC 2.5.1.17), at 6,338,252 to 6,338,833
_5519
Pf6N2E2_5520 - Sensory box histidine kinase, at 6,338,836 to 6,340,872
_5520
Pf6N2E2_5521 - 2-dehydropantoate 2-reductase (EC 1.1.1.169), at 6,340,919 to 6,341,836
_5521
Position (kb)
6338
6339
6340
6341 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6337.870 kb on + strand, within Pf6N2E2_5518 at 6337.959 kb on + strand, within Pf6N2E2_5518 at 6337.959 kb on + strand, within Pf6N2E2_5518 at 6337.960 kb on - strand, within Pf6N2E2_5518 at 6337.960 kb on - strand, within Pf6N2E2_5518 at 6338.009 kb on - strand, within Pf6N2E2_5518 at 6338.018 kb on + strand, within Pf6N2E2_5518 at 6338.018 kb on + strand, within Pf6N2E2_5518 at 6338.018 kb on + strand, within Pf6N2E2_5518 at 6338.018 kb on + strand, within Pf6N2E2_5518 at 6338.018 kb on + strand, within Pf6N2E2_5518 at 6338.019 kb on - strand, within Pf6N2E2_5518 at 6338.356 kb on + strand, within Pf6N2E2_5519 at 6338.356 kb on + strand, within Pf6N2E2_5519 at 6338.357 kb on - strand, within Pf6N2E2_5519 at 6338.358 kb on + strand, within Pf6N2E2_5519 at 6338.358 kb on + strand, within Pf6N2E2_5519 at 6338.358 kb on + strand, within Pf6N2E2_5519 at 6338.359 kb on - strand, within Pf6N2E2_5519 at 6338.457 kb on - strand, within Pf6N2E2_5519 at 6338.569 kb on - strand, within Pf6N2E2_5519 at 6338.690 kb on + strand, within Pf6N2E2_5519 at 6338.690 kb on + strand, within Pf6N2E2_5519 at 6338.691 kb on - strand, within Pf6N2E2_5519 at 6338.714 kb on - strand, within Pf6N2E2_5519 at 6338.808 kb on + strand at 6338.972 kb on + strand at 6339.129 kb on + strand, within Pf6N2E2_5520 at 6339.758 kb on + strand, within Pf6N2E2_5520 at 6339.951 kb on - strand, within Pf6N2E2_5520 at 6339.951 kb on - strand, within Pf6N2E2_5520 at 6340.010 kb on + strand, within Pf6N2E2_5520 at 6340.010 kb on + strand, within Pf6N2E2_5520 at 6340.010 kb on + strand, within Pf6N2E2_5520 at 6340.011 kb on - strand, within Pf6N2E2_5520 at 6340.121 kb on + strand, within Pf6N2E2_5520 at 6340.253 kb on + strand, within Pf6N2E2_5520 at 6340.253 kb on + strand, within Pf6N2E2_5520 at 6340.253 kb on + strand, within Pf6N2E2_5520 at 6340.253 kb on + strand, within Pf6N2E2_5520 at 6340.254 kb on - strand, within Pf6N2E2_5520 at 6340.254 kb on - strand, within Pf6N2E2_5520 at 6340.254 kb on - strand, within Pf6N2E2_5520 at 6340.254 kb on - strand, within Pf6N2E2_5520 at 6340.254 kb on - strand, within Pf6N2E2_5520 at 6340.400 kb on + strand, within Pf6N2E2_5520 at 6340.401 kb on - strand at 6340.401 kb on - strand, within Pf6N2E2_5520 at 6340.401 kb on - strand, within Pf6N2E2_5520 at 6340.401 kb on - strand, within Pf6N2E2_5520 at 6340.435 kb on + strand, within Pf6N2E2_5520 at 6340.516 kb on + strand, within Pf6N2E2_5520 at 6340.516 kb on + strand, within Pf6N2E2_5520 at 6340.516 kb on + strand, within Pf6N2E2_5520 at 6340.516 kb on + strand, within Pf6N2E2_5520 at 6340.517 kb on - strand, within Pf6N2E2_5520 at 6340.619 kb on + strand, within Pf6N2E2_5520 at 6340.620 kb on - strand, within Pf6N2E2_5520 at 6340.751 kb on + strand at 6340.751 kb on + strand at 6340.751 kb on + strand at 6340.751 kb on + strand at 6340.752 kb on - strand at 6340.752 kb on - strand at 6340.754 kb on + strand at 6340.754 kb on + strand at 6340.755 kb on - strand at 6340.755 kb on - strand at 6340.767 kb on + strand at 6340.767 kb on + strand at 6340.896 kb on - strand at 6340.896 kb on - strand at 6341.037 kb on + strand, within Pf6N2E2_5521 at 6341.037 kb on + strand, within Pf6N2E2_5521 at 6341.037 kb on + strand, within Pf6N2E2_5521 at 6341.037 kb on + strand, within Pf6N2E2_5521 at 6341.037 kb on + strand, within Pf6N2E2_5521 at 6341.037 kb on + strand, within Pf6N2E2_5521 at 6341.037 kb on + strand, within Pf6N2E2_5521 at 6341.038 kb on - strand, within Pf6N2E2_5521 at 6341.038 kb on - strand, within Pf6N2E2_5521 at 6341.076 kb on + strand, within Pf6N2E2_5521 at 6341.076 kb on + strand, within Pf6N2E2_5521 at 6341.088 kb on + strand, within Pf6N2E2_5521 at 6341.088 kb on + strand, within Pf6N2E2_5521 at 6341.088 kb on + strand, within Pf6N2E2_5521 at 6341.132 kb on + strand, within Pf6N2E2_5521 at 6341.400 kb on + strand, within Pf6N2E2_5521 at 6341.417 kb on + strand, within Pf6N2E2_5521 at 6341.536 kb on + strand, within Pf6N2E2_5521 at 6341.536 kb on + strand, within Pf6N2E2_5521 at 6341.536 kb on + strand, within Pf6N2E2_5521 at 6341.536 kb on + strand, within Pf6N2E2_5521 at 6341.536 kb on + strand, within Pf6N2E2_5521 at 6341.537 kb on - strand, within Pf6N2E2_5521 at 6341.537 kb on - strand, within Pf6N2E2_5521 at 6341.537 kb on - strand, within Pf6N2E2_5521 at 6341.537 kb on - strand, within Pf6N2E2_5521 at 6341.537 kb on - strand, within Pf6N2E2_5521 at 6341.595 kb on + strand, within Pf6N2E2_5521 at 6341.612 kb on + strand, within Pf6N2E2_5521 at 6341.845 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 6,337,870 + Pf6N2E2_5518 0.73 -0.5 6,337,959 + Pf6N2E2_5518 0.83 -1.0 6,337,959 + Pf6N2E2_5518 0.83 -0.4 6,337,960 - Pf6N2E2_5518 0.83 -0.3 6,337,960 - Pf6N2E2_5518 0.83 -1.2 6,338,009 - Pf6N2E2_5518 0.88 +0.1 6,338,018 + Pf6N2E2_5518 0.89 -0.3 6,338,018 + Pf6N2E2_5518 0.89 +0.3 6,338,018 + Pf6N2E2_5518 0.89 -0.0 6,338,018 + Pf6N2E2_5518 0.89 +0.1 6,338,018 + Pf6N2E2_5518 0.89 +0.6 6,338,019 - Pf6N2E2_5518 0.89 -1.9 6,338,356 + Pf6N2E2_5519 0.18 -0.1 6,338,356 + Pf6N2E2_5519 0.18 +0.1 6,338,357 - Pf6N2E2_5519 0.18 +0.1 6,338,358 + Pf6N2E2_5519 0.18 +1.8 6,338,358 + Pf6N2E2_5519 0.18 -0.8 6,338,358 + Pf6N2E2_5519 0.18 +0.3 6,338,359 - Pf6N2E2_5519 0.18 -0.2 6,338,457 - Pf6N2E2_5519 0.35 -0.3 6,338,569 - Pf6N2E2_5519 0.54 +0.9 6,338,690 + Pf6N2E2_5519 0.75 +1.2 6,338,690 + Pf6N2E2_5519 0.75 +0.2 6,338,691 - Pf6N2E2_5519 0.75 +0.4 6,338,714 - Pf6N2E2_5519 0.79 -0.9 6,338,808 + -0.6 6,338,972 + -1.4 6,339,129 + Pf6N2E2_5520 0.14 -0.9 6,339,758 + Pf6N2E2_5520 0.45 -0.2 6,339,951 - Pf6N2E2_5520 0.55 +1.4 6,339,951 - Pf6N2E2_5520 0.55 -0.6 6,340,010 + Pf6N2E2_5520 0.58 -2.3 6,340,010 + Pf6N2E2_5520 0.58 -2.2 6,340,010 + Pf6N2E2_5520 0.58 +1.4 6,340,011 - Pf6N2E2_5520 0.58 -0.9 6,340,121 + Pf6N2E2_5520 0.63 -1.6 6,340,253 + Pf6N2E2_5520 0.70 -0.5 6,340,253 + Pf6N2E2_5520 0.70 +1.4 6,340,253 + Pf6N2E2_5520 0.70 -1.8 6,340,253 + Pf6N2E2_5520 0.70 -2.4 6,340,254 - Pf6N2E2_5520 0.70 -1.3 6,340,254 - Pf6N2E2_5520 0.70 -1.6 6,340,254 - Pf6N2E2_5520 0.70 -1.3 6,340,254 - Pf6N2E2_5520 0.70 -1.9 6,340,254 - Pf6N2E2_5520 0.70 -0.2 6,340,400 + Pf6N2E2_5520 0.77 -0.3 6,340,401 - -0.9 6,340,401 - Pf6N2E2_5520 0.77 -0.6 6,340,401 - Pf6N2E2_5520 0.77 -1.9 6,340,401 - Pf6N2E2_5520 0.77 -0.2 6,340,435 + Pf6N2E2_5520 0.78 -2.9 6,340,516 + Pf6N2E2_5520 0.82 -1.6 6,340,516 + Pf6N2E2_5520 0.82 -1.9 6,340,516 + Pf6N2E2_5520 0.82 -1.6 6,340,516 + Pf6N2E2_5520 0.82 -2.9 6,340,517 - Pf6N2E2_5520 0.83 -2.0 6,340,619 + Pf6N2E2_5520 0.88 -0.2 6,340,620 - Pf6N2E2_5520 0.88 -2.0 6,340,751 + -0.6 6,340,751 + -1.2 6,340,751 + -1.9 6,340,751 + -1.2 6,340,752 - -1.9 6,340,752 - -0.6 6,340,754 + -1.3 6,340,754 + -2.5 6,340,755 - -0.6 6,340,755 - -0.8 6,340,767 + -1.5 6,340,767 + -1.4 6,340,896 - -0.9 6,340,896 - -0.5 6,341,037 + Pf6N2E2_5521 0.13 +1.1 6,341,037 + Pf6N2E2_5521 0.13 -0.5 6,341,037 + Pf6N2E2_5521 0.13 +0.3 6,341,037 + Pf6N2E2_5521 0.13 -0.3 6,341,037 + Pf6N2E2_5521 0.13 -0.5 6,341,037 + Pf6N2E2_5521 0.13 +0.5 6,341,037 + Pf6N2E2_5521 0.13 +1.5 6,341,038 - Pf6N2E2_5521 0.13 +0.7 6,341,038 - Pf6N2E2_5521 0.13 -1.2 6,341,076 + Pf6N2E2_5521 0.17 +1.4 6,341,076 + Pf6N2E2_5521 0.17 -0.4 6,341,088 + Pf6N2E2_5521 0.18 +0.4 6,341,088 + Pf6N2E2_5521 0.18 +0.2 6,341,088 + Pf6N2E2_5521 0.18 -0.1 6,341,132 + Pf6N2E2_5521 0.23 +0.8 6,341,400 + Pf6N2E2_5521 0.52 -1.9 6,341,417 + Pf6N2E2_5521 0.54 +0.7 6,341,536 + Pf6N2E2_5521 0.67 +1.0 6,341,536 + Pf6N2E2_5521 0.67 +0.9 6,341,536 + Pf6N2E2_5521 0.67 +1.4 6,341,536 + Pf6N2E2_5521 0.67 +0.4 6,341,536 + Pf6N2E2_5521 0.67 +0.7 6,341,537 - Pf6N2E2_5521 0.67 +1.0 6,341,537 - Pf6N2E2_5521 0.67 +0.9 6,341,537 - Pf6N2E2_5521 0.67 +0.8 6,341,537 - Pf6N2E2_5521 0.67 -0.1 6,341,537 - Pf6N2E2_5521 0.67 -0.0 6,341,595 + Pf6N2E2_5521 0.74 -0.9 6,341,612 + Pf6N2E2_5521 0.75 +0.4 6,341,845 + +1.0
Or see this region's nucleotide sequence