Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5391 and Pf6N2E2_5392 are separated by 168 nucleotides Pf6N2E2_5392 and Pf6N2E2_5393 overlap by 4 nucleotides Pf6N2E2_5393 and Pf6N2E2_5394 are separated by 3 nucleotides Pf6N2E2_5394 and Pf6N2E2_5395 are separated by 298 nucleotides
Pf6N2E2_5391 - Predicted ATPase related to phosphate starvation-inducible protein PhoH, at 6,197,912 to 6,199,306
_5391
Pf6N2E2_5392 - Molybdenum cofactor biosynthesis protein MoaC, at 6,199,475 to 6,199,948
_5392
Pf6N2E2_5393 - Molybdenum cofactor biosynthesis protein MoaD, at 6,199,945 to 6,200,187
_5393
Pf6N2E2_5394 - Molybdenum cofactor biosynthesis protein MoaE, at 6,200,191 to 6,200,643
_5394
Pf6N2E2_5395 - Arginine/ornithine ABC transporter, periplasmic arginine/ornithine binding protein, at 6,200,942 to 6,201,676
_5395
Position (kb)
6199
6200 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6198.689 kb on + strand, within Pf6N2E2_5391 at 6198.689 kb on + strand, within Pf6N2E2_5391 at 6198.690 kb on - strand, within Pf6N2E2_5391 at 6198.690 kb on - strand, within Pf6N2E2_5391 at 6198.690 kb on - strand, within Pf6N2E2_5391 at 6198.690 kb on - strand, within Pf6N2E2_5391 at 6198.690 kb on - strand, within Pf6N2E2_5391 at 6198.690 kb on - strand, within Pf6N2E2_5391 at 6198.983 kb on + strand, within Pf6N2E2_5391 at 6198.983 kb on + strand, within Pf6N2E2_5391 at 6198.983 kb on + strand, within Pf6N2E2_5391 at 6198.983 kb on + strand, within Pf6N2E2_5391 at 6198.983 kb on + strand, within Pf6N2E2_5391 at 6198.983 kb on + strand, within Pf6N2E2_5391 at 6198.984 kb on - strand, within Pf6N2E2_5391 at 6198.984 kb on - strand, within Pf6N2E2_5391 at 6198.984 kb on - strand, within Pf6N2E2_5391 at 6198.984 kb on - strand, within Pf6N2E2_5391 at 6198.984 kb on - strand, within Pf6N2E2_5391 at 6198.984 kb on - strand, within Pf6N2E2_5391 at 6199.025 kb on + strand, within Pf6N2E2_5391 at 6199.025 kb on + strand, within Pf6N2E2_5391 at 6199.025 kb on + strand, within Pf6N2E2_5391 at 6199.026 kb on - strand, within Pf6N2E2_5391 at 6199.026 kb on - strand, within Pf6N2E2_5391 at 6199.026 kb on - strand, within Pf6N2E2_5391 at 6199.026 kb on - strand, within Pf6N2E2_5391 at 6199.026 kb on - strand, within Pf6N2E2_5391 at 6199.026 kb on - strand, within Pf6N2E2_5391 at 6199.168 kb on + strand at 6199.175 kb on + strand at 6199.175 kb on + strand at 6199.175 kb on + strand at 6199.175 kb on + strand at 6199.175 kb on + strand at 6199.175 kb on + strand at 6199.175 kb on + strand at 6199.176 kb on - strand at 6199.176 kb on - strand at 6199.176 kb on - strand at 6199.176 kb on - strand at 6199.176 kb on - strand at 6199.205 kb on + strand at 6199.205 kb on + strand at 6199.205 kb on + strand at 6199.205 kb on + strand at 6199.206 kb on - strand at 6199.206 kb on - strand at 6199.332 kb on - strand at 6199.347 kb on - strand at 6199.347 kb on - strand at 6199.347 kb on - strand at 6199.347 kb on - strand at 6199.347 kb on - strand at 6199.353 kb on + strand at 6199.353 kb on + strand at 6199.354 kb on - strand at 6199.354 kb on - strand at 6199.354 kb on - strand at 6199.435 kb on + strand at 6199.435 kb on + strand at 6199.443 kb on + strand at 6199.443 kb on + strand at 6199.444 kb on - strand at 6199.469 kb on - strand at 6199.755 kb on + strand, within Pf6N2E2_5392 at 6199.844 kb on + strand, within Pf6N2E2_5392 at 6199.844 kb on + strand, within Pf6N2E2_5392 at 6199.844 kb on + strand, within Pf6N2E2_5392 at 6199.845 kb on - strand, within Pf6N2E2_5392 at 6199.845 kb on - strand, within Pf6N2E2_5392 at 6200.533 kb on + strand, within Pf6N2E2_5394 at 6200.533 kb on + strand, within Pf6N2E2_5394 at 6200.534 kb on - strand, within Pf6N2E2_5394 at 6200.534 kb on - strand, within Pf6N2E2_5394 at 6200.641 kb on + strand at 6200.641 kb on + strand at 6200.642 kb on - strand at 6200.642 kb on - strand at 6200.719 kb on + strand at 6200.719 kb on + strand at 6200.719 kb on + strand at 6200.719 kb on + strand at 6200.719 kb on + strand at 6200.720 kb on - strand at 6200.720 kb on - strand at 6200.720 kb on - strand at 6200.720 kb on - strand at 6200.720 kb on - strand at 6200.720 kb on - strand at 6200.832 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 6,198,689 + Pf6N2E2_5391 0.56 +0.1 6,198,689 + Pf6N2E2_5391 0.56 -0.5 6,198,690 - Pf6N2E2_5391 0.56 +0.8 6,198,690 - Pf6N2E2_5391 0.56 -0.2 6,198,690 - Pf6N2E2_5391 0.56 +0.6 6,198,690 - Pf6N2E2_5391 0.56 -0.6 6,198,690 - Pf6N2E2_5391 0.56 -1.8 6,198,690 - Pf6N2E2_5391 0.56 -0.3 6,198,983 + Pf6N2E2_5391 0.77 -0.3 6,198,983 + Pf6N2E2_5391 0.77 +1.4 6,198,983 + Pf6N2E2_5391 0.77 -0.3 6,198,983 + Pf6N2E2_5391 0.77 +1.4 6,198,983 + Pf6N2E2_5391 0.77 -1.4 6,198,983 + Pf6N2E2_5391 0.77 +0.4 6,198,984 - Pf6N2E2_5391 0.77 -0.6 6,198,984 - Pf6N2E2_5391 0.77 -1.1 6,198,984 - Pf6N2E2_5391 0.77 -0.1 6,198,984 - Pf6N2E2_5391 0.77 +1.4 6,198,984 - Pf6N2E2_5391 0.77 -0.7 6,198,984 - Pf6N2E2_5391 0.77 -0.7 6,199,025 + Pf6N2E2_5391 0.80 +0.0 6,199,025 + Pf6N2E2_5391 0.80 -0.6 6,199,025 + Pf6N2E2_5391 0.80 -0.9 6,199,026 - Pf6N2E2_5391 0.80 -0.9 6,199,026 - Pf6N2E2_5391 0.80 +2.3 6,199,026 - Pf6N2E2_5391 0.80 +1.1 6,199,026 - Pf6N2E2_5391 0.80 -0.2 6,199,026 - Pf6N2E2_5391 0.80 -0.5 6,199,026 - Pf6N2E2_5391 0.80 -1.0 6,199,168 + +1.6 6,199,175 + -0.6 6,199,175 + -0.9 6,199,175 + -0.0 6,199,175 + -0.2 6,199,175 + -0.2 6,199,175 + -0.6 6,199,175 + -0.4 6,199,176 - -0.9 6,199,176 - -0.6 6,199,176 - +1.1 6,199,176 - +0.6 6,199,176 - +1.4 6,199,205 + -0.9 6,199,205 + +0.7 6,199,205 + -0.6 6,199,205 + -0.6 6,199,206 - -0.1 6,199,206 - -0.1 6,199,332 - +0.3 6,199,347 - +0.4 6,199,347 - +0.2 6,199,347 - +0.1 6,199,347 - -0.1 6,199,347 - -0.8 6,199,353 + -0.4 6,199,353 + +0.2 6,199,354 - +0.5 6,199,354 - +2.4 6,199,354 - +1.0 6,199,435 + -0.4 6,199,435 + +0.4 6,199,443 + +0.1 6,199,443 + -0.8 6,199,444 - -1.0 6,199,469 - -1.2 6,199,755 + Pf6N2E2_5392 0.59 -0.2 6,199,844 + Pf6N2E2_5392 0.78 -3.4 6,199,844 + Pf6N2E2_5392 0.78 -1.4 6,199,844 + Pf6N2E2_5392 0.78 +1.4 6,199,845 - Pf6N2E2_5392 0.78 -1.0 6,199,845 - Pf6N2E2_5392 0.78 -1.9 6,200,533 + Pf6N2E2_5394 0.75 -2.5 6,200,533 + Pf6N2E2_5394 0.75 -3.1 6,200,534 - Pf6N2E2_5394 0.76 -2.6 6,200,534 - Pf6N2E2_5394 0.76 -1.4 6,200,641 + +1.4 6,200,641 + +0.0 6,200,642 - +0.4 6,200,642 - +1.1 6,200,719 + -0.6 6,200,719 + +1.1 6,200,719 + +1.4 6,200,719 + -0.9 6,200,719 + +0.6 6,200,720 - -0.6 6,200,720 - +2.2 6,200,720 - -0.2 6,200,720 - -0.5 6,200,720 - +0.4 6,200,720 - +0.4 6,200,832 + +1.9
Or see this region's nucleotide sequence