Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5308 and Pf6N2E2_5309 are separated by 136 nucleotides Pf6N2E2_5309 and Pf6N2E2_5310 are separated by 180 nucleotides
Pf6N2E2_5308 - Transcriptional regulator, AraC family, at 6,102,281 to 6,103,156
_5308
Pf6N2E2_5309 - Permease of the drug/metabolite transporter (DMT) superfamily, at 6,103,293 to 6,104,195
_5309
Pf6N2E2_5310 - Glyoxylate reductase (EC 1.1.1.79) / Glyoxylate reductase (EC 1.1.1.26) / Hydroxypyruvate reductase (EC 1.1.1.81); 2-ketoaldonate reductase, broad specificity (EC 1.1.1.215) (EC 1.1.1.-), at 6,104,376 to 6,105,347
_5310
Position (kb)
6103
6104
6105 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 6102.303 kb on + strand at 6102.453 kb on + strand, within Pf6N2E2_5308 at 6102.453 kb on + strand, within Pf6N2E2_5308 at 6102.453 kb on + strand, within Pf6N2E2_5308 at 6102.453 kb on + strand, within Pf6N2E2_5308 at 6102.453 kb on + strand, within Pf6N2E2_5308 at 6102.453 kb on + strand, within Pf6N2E2_5308 at 6102.453 kb on + strand, within Pf6N2E2_5308 at 6102.453 kb on + strand, within Pf6N2E2_5308 at 6102.453 kb on + strand, within Pf6N2E2_5308 at 6102.453 kb on + strand, within Pf6N2E2_5308 at 6102.453 kb on + strand, within Pf6N2E2_5308 at 6102.453 kb on + strand, within Pf6N2E2_5308 at 6102.453 kb on + strand, within Pf6N2E2_5308 at 6102.453 kb on + strand, within Pf6N2E2_5308 at 6102.453 kb on + strand, within Pf6N2E2_5308 at 6102.454 kb on - strand, within Pf6N2E2_5308 at 6102.454 kb on - strand, within Pf6N2E2_5308 at 6102.454 kb on - strand, within Pf6N2E2_5308 at 6102.454 kb on - strand, within Pf6N2E2_5308 at 6102.454 kb on - strand, within Pf6N2E2_5308 at 6102.454 kb on - strand, within Pf6N2E2_5308 at 6102.454 kb on - strand, within Pf6N2E2_5308 at 6102.454 kb on - strand, within Pf6N2E2_5308 at 6102.454 kb on - strand, within Pf6N2E2_5308 at 6102.454 kb on - strand, within Pf6N2E2_5308 at 6102.454 kb on - strand, within Pf6N2E2_5308 at 6102.454 kb on - strand, within Pf6N2E2_5308 at 6102.454 kb on - strand, within Pf6N2E2_5308 at 6102.454 kb on - strand, within Pf6N2E2_5308 at 6102.504 kb on + strand, within Pf6N2E2_5308 at 6102.573 kb on + strand, within Pf6N2E2_5308 at 6102.573 kb on + strand, within Pf6N2E2_5308 at 6102.573 kb on + strand, within Pf6N2E2_5308 at 6102.574 kb on - strand, within Pf6N2E2_5308 at 6102.815 kb on + strand, within Pf6N2E2_5308 at 6102.815 kb on + strand, within Pf6N2E2_5308 at 6102.855 kb on + strand, within Pf6N2E2_5308 at 6102.855 kb on + strand, within Pf6N2E2_5308 at 6102.855 kb on + strand, within Pf6N2E2_5308 at 6102.856 kb on - strand, within Pf6N2E2_5308 at 6102.856 kb on - strand, within Pf6N2E2_5308 at 6102.856 kb on - strand, within Pf6N2E2_5308 at 6102.856 kb on - strand, within Pf6N2E2_5308 at 6102.856 kb on - strand, within Pf6N2E2_5308 at 6102.971 kb on - strand, within Pf6N2E2_5308 at 6102.990 kb on + strand, within Pf6N2E2_5308 at 6102.990 kb on + strand, within Pf6N2E2_5308 at 6102.990 kb on + strand, within Pf6N2E2_5308 at 6102.990 kb on + strand, within Pf6N2E2_5308 at 6102.990 kb on + strand, within Pf6N2E2_5308 at 6103.003 kb on + strand, within Pf6N2E2_5308 at 6103.003 kb on + strand, within Pf6N2E2_5308 at 6103.004 kb on - strand, within Pf6N2E2_5308 at 6103.004 kb on - strand, within Pf6N2E2_5308 at 6103.004 kb on - strand, within Pf6N2E2_5308 at 6103.023 kb on + strand, within Pf6N2E2_5308 at 6103.136 kb on + strand at 6103.136 kb on + strand at 6103.182 kb on + strand at 6103.182 kb on + strand at 6103.182 kb on + strand at 6103.183 kb on - strand at 6103.183 kb on - strand at 6103.183 kb on - strand at 6103.183 kb on - strand at 6103.183 kb on - strand at 6103.183 kb on - strand at 6103.234 kb on - strand at 6103.308 kb on + strand at 6103.308 kb on + strand at 6103.308 kb on + strand at 6103.308 kb on + strand at 6103.308 kb on + strand at 6103.309 kb on - strand at 6103.309 kb on - strand at 6103.317 kb on - strand at 6103.518 kb on - strand, within Pf6N2E2_5309 at 6103.518 kb on - strand, within Pf6N2E2_5309 at 6103.854 kb on + strand, within Pf6N2E2_5309 at 6103.855 kb on - strand, within Pf6N2E2_5309 at 6103.927 kb on - strand, within Pf6N2E2_5309 at 6103.944 kb on + strand, within Pf6N2E2_5309 at 6103.944 kb on + strand, within Pf6N2E2_5309 at 6103.944 kb on + strand, within Pf6N2E2_5309 at 6103.945 kb on - strand, within Pf6N2E2_5309 at 6104.025 kb on + strand, within Pf6N2E2_5309 at 6104.290 kb on + strand at 6104.291 kb on - strand at 6104.291 kb on - strand at 6104.377 kb on + strand at 6104.377 kb on + strand at 6104.377 kb on + strand at 6104.377 kb on + strand at 6104.378 kb on - strand at 6104.378 kb on - strand at 6104.378 kb on - strand at 6104.560 kb on + strand, within Pf6N2E2_5310 at 6104.560 kb on + strand, within Pf6N2E2_5310 at 6104.560 kb on + strand, within Pf6N2E2_5310 at 6104.561 kb on - strand, within Pf6N2E2_5310 at 6104.561 kb on - strand, within Pf6N2E2_5310 at 6104.827 kb on + strand, within Pf6N2E2_5310 at 6104.827 kb on + strand, within Pf6N2E2_5310 at 6104.919 kb on + strand, within Pf6N2E2_5310 at 6104.919 kb on + strand, within Pf6N2E2_5310 at 6104.919 kb on + strand, within Pf6N2E2_5310 at 6104.919 kb on + strand, within Pf6N2E2_5310 at 6104.920 kb on - strand, within Pf6N2E2_5310 at 6104.920 kb on - strand, within Pf6N2E2_5310 at 6104.920 kb on - strand, within Pf6N2E2_5310 at 6104.920 kb on - strand, within Pf6N2E2_5310 at 6104.920 kb on - strand, within Pf6N2E2_5310 at 6104.920 kb on - strand, within Pf6N2E2_5310 at 6105.054 kb on + strand, within Pf6N2E2_5310 at 6105.054 kb on + strand, within Pf6N2E2_5310 at 6105.055 kb on - strand, within Pf6N2E2_5310 at 6105.055 kb on - strand, within Pf6N2E2_5310 at 6105.055 kb on - strand, within Pf6N2E2_5310 at 6105.055 kb on - strand, within Pf6N2E2_5310 at 6105.055 kb on - strand, within Pf6N2E2_5310 at 6105.097 kb on + strand, within Pf6N2E2_5310 at 6105.097 kb on + strand, within Pf6N2E2_5310 at 6105.109 kb on + strand, within Pf6N2E2_5310 at 6105.109 kb on + strand, within Pf6N2E2_5310 at 6105.109 kb on + strand, within Pf6N2E2_5310 at 6105.109 kb on + strand, within Pf6N2E2_5310 at 6105.109 kb on + strand, within Pf6N2E2_5310 at 6105.109 kb on + strand, within Pf6N2E2_5310 at 6105.109 kb on + strand, within Pf6N2E2_5310 at 6105.109 kb on + strand, within Pf6N2E2_5310 at 6105.109 kb on + strand, within Pf6N2E2_5310 at 6105.109 kb on + strand, within Pf6N2E2_5310 at 6105.110 kb on - strand, within Pf6N2E2_5310 at 6105.110 kb on - strand, within Pf6N2E2_5310 at 6105.110 kb on - strand, within Pf6N2E2_5310 at 6105.110 kb on - strand, within Pf6N2E2_5310 at 6105.110 kb on - strand, within Pf6N2E2_5310 at 6105.110 kb on - strand, within Pf6N2E2_5310 at 6105.110 kb on - strand, within Pf6N2E2_5310
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 6,102,303 + -2.8 6,102,453 + Pf6N2E2_5308 0.20 -1.4 6,102,453 + Pf6N2E2_5308 0.20 -2.8 6,102,453 + Pf6N2E2_5308 0.20 -2.5 6,102,453 + Pf6N2E2_5308 0.20 -3.9 6,102,453 + Pf6N2E2_5308 0.20 -2.5 6,102,453 + Pf6N2E2_5308 0.20 -3.4 6,102,453 + Pf6N2E2_5308 0.20 -2.0 6,102,453 + Pf6N2E2_5308 0.20 -3.3 6,102,453 + Pf6N2E2_5308 0.20 -3.5 6,102,453 + Pf6N2E2_5308 0.20 -2.4 6,102,453 + Pf6N2E2_5308 0.20 -2.4 6,102,453 + Pf6N2E2_5308 0.20 -0.9 6,102,453 + Pf6N2E2_5308 0.20 -2.2 6,102,453 + Pf6N2E2_5308 0.20 -3.3 6,102,453 + Pf6N2E2_5308 0.20 -2.0 6,102,454 - Pf6N2E2_5308 0.20 -3.6 6,102,454 - Pf6N2E2_5308 0.20 -4.6 6,102,454 - Pf6N2E2_5308 0.20 -1.8 6,102,454 - Pf6N2E2_5308 0.20 -3.0 6,102,454 - Pf6N2E2_5308 0.20 -1.8 6,102,454 - Pf6N2E2_5308 0.20 -3.9 6,102,454 - Pf6N2E2_5308 0.20 -2.9 6,102,454 - Pf6N2E2_5308 0.20 -3.4 6,102,454 - Pf6N2E2_5308 0.20 -1.9 6,102,454 - Pf6N2E2_5308 0.20 -2.2 6,102,454 - Pf6N2E2_5308 0.20 -3.6 6,102,454 - Pf6N2E2_5308 0.20 -1.4 6,102,454 - Pf6N2E2_5308 0.20 -1.8 6,102,454 - Pf6N2E2_5308 0.20 -3.8 6,102,504 + Pf6N2E2_5308 0.25 -1.7 6,102,573 + Pf6N2E2_5308 0.33 -1.9 6,102,573 + Pf6N2E2_5308 0.33 -5.0 6,102,573 + Pf6N2E2_5308 0.33 -2.6 6,102,574 - Pf6N2E2_5308 0.33 -2.0 6,102,815 + Pf6N2E2_5308 0.61 +1.4 6,102,815 + Pf6N2E2_5308 0.61 -1.6 6,102,855 + Pf6N2E2_5308 0.66 -1.2 6,102,855 + Pf6N2E2_5308 0.66 -2.1 6,102,855 + Pf6N2E2_5308 0.66 -2.7 6,102,856 - Pf6N2E2_5308 0.66 -3.2 6,102,856 - Pf6N2E2_5308 0.66 -3.8 6,102,856 - Pf6N2E2_5308 0.66 -0.9 6,102,856 - Pf6N2E2_5308 0.66 -2.9 6,102,856 - Pf6N2E2_5308 0.66 -3.4 6,102,971 - Pf6N2E2_5308 0.79 -2.4 6,102,990 + Pf6N2E2_5308 0.81 -0.2 6,102,990 + Pf6N2E2_5308 0.81 -3.1 6,102,990 + Pf6N2E2_5308 0.81 -4.9 6,102,990 + Pf6N2E2_5308 0.81 -2.2 6,102,990 + Pf6N2E2_5308 0.81 -2.5 6,103,003 + Pf6N2E2_5308 0.82 -2.6 6,103,003 + Pf6N2E2_5308 0.82 -1.9 6,103,004 - Pf6N2E2_5308 0.83 -2.0 6,103,004 - Pf6N2E2_5308 0.83 -2.8 6,103,004 - Pf6N2E2_5308 0.83 +1.4 6,103,023 + Pf6N2E2_5308 0.85 -3.2 6,103,136 + -2.9 6,103,136 + -1.5 6,103,182 + -0.8 6,103,182 + -0.6 6,103,182 + +0.4 6,103,183 - -0.4 6,103,183 - -0.6 6,103,183 - -0.5 6,103,183 - -1.4 6,103,183 - -0.5 6,103,183 - -0.2 6,103,234 - -2.5 6,103,308 + -2.1 6,103,308 + -3.3 6,103,308 + -1.9 6,103,308 + -2.4 6,103,308 + -1.2 6,103,309 - -1.9 6,103,309 - +0.4 6,103,317 - -3.9 6,103,518 - Pf6N2E2_5309 0.25 -1.6 6,103,518 - Pf6N2E2_5309 0.25 -2.7 6,103,854 + Pf6N2E2_5309 0.62 -2.6 6,103,855 - Pf6N2E2_5309 0.62 -2.7 6,103,927 - Pf6N2E2_5309 0.70 -1.9 6,103,944 + Pf6N2E2_5309 0.72 -3.8 6,103,944 + Pf6N2E2_5309 0.72 -3.6 6,103,944 + Pf6N2E2_5309 0.72 -3.1 6,103,945 - Pf6N2E2_5309 0.72 -1.4 6,104,025 + Pf6N2E2_5309 0.81 -2.7 6,104,290 + +1.7 6,104,291 - -0.5 6,104,291 - -1.7 6,104,377 + -0.6 6,104,377 + +0.3 6,104,377 + +0.8 6,104,377 + +0.0 6,104,378 - +2.2 6,104,378 - -0.8 6,104,378 - -0.2 6,104,560 + Pf6N2E2_5310 0.19 +0.2 6,104,560 + Pf6N2E2_5310 0.19 -0.2 6,104,560 + Pf6N2E2_5310 0.19 -0.7 6,104,561 - Pf6N2E2_5310 0.19 +0.2 6,104,561 - Pf6N2E2_5310 0.19 +1.2 6,104,827 + Pf6N2E2_5310 0.46 -0.5 6,104,827 + Pf6N2E2_5310 0.46 -0.4 6,104,919 + Pf6N2E2_5310 0.56 +0.4 6,104,919 + Pf6N2E2_5310 0.56 +0.6 6,104,919 + Pf6N2E2_5310 0.56 +0.6 6,104,919 + Pf6N2E2_5310 0.56 +0.6 6,104,920 - Pf6N2E2_5310 0.56 -1.3 6,104,920 - Pf6N2E2_5310 0.56 -1.6 6,104,920 - Pf6N2E2_5310 0.56 +0.5 6,104,920 - Pf6N2E2_5310 0.56 -0.4 6,104,920 - Pf6N2E2_5310 0.56 -0.1 6,104,920 - Pf6N2E2_5310 0.56 +0.8 6,105,054 + Pf6N2E2_5310 0.70 -0.4 6,105,054 + Pf6N2E2_5310 0.70 +0.0 6,105,055 - Pf6N2E2_5310 0.70 +0.1 6,105,055 - Pf6N2E2_5310 0.70 -0.1 6,105,055 - Pf6N2E2_5310 0.70 +2.0 6,105,055 - Pf6N2E2_5310 0.70 -0.8 6,105,055 - Pf6N2E2_5310 0.70 -1.4 6,105,097 + Pf6N2E2_5310 0.74 +0.3 6,105,097 + Pf6N2E2_5310 0.74 -0.3 6,105,109 + Pf6N2E2_5310 0.75 -1.2 6,105,109 + Pf6N2E2_5310 0.75 +0.7 6,105,109 + Pf6N2E2_5310 0.75 -1.2 6,105,109 + Pf6N2E2_5310 0.75 +1.4 6,105,109 + Pf6N2E2_5310 0.75 +0.1 6,105,109 + Pf6N2E2_5310 0.75 -0.5 6,105,109 + Pf6N2E2_5310 0.75 -1.3 6,105,109 + Pf6N2E2_5310 0.75 -0.3 6,105,109 + Pf6N2E2_5310 0.75 -0.9 6,105,109 + Pf6N2E2_5310 0.75 -0.8 6,105,110 - Pf6N2E2_5310 0.76 -0.6 6,105,110 - Pf6N2E2_5310 0.76 +2.0 6,105,110 - Pf6N2E2_5310 0.76 -0.6 6,105,110 - Pf6N2E2_5310 0.76 +0.3 6,105,110 - Pf6N2E2_5310 0.76 +1.3 6,105,110 - Pf6N2E2_5310 0.76 -0.6 6,105,110 - Pf6N2E2_5310 0.76 -0.2
Or see this region's nucleotide sequence