Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5175 and Pf6N2E2_5176 are separated by 2 nucleotides Pf6N2E2_5176 and Pf6N2E2_5177 are separated by 45 nucleotides Pf6N2E2_5177 and Pf6N2E2_5178 are separated by 145 nucleotides
Pf6N2E2_5175 - Acetolactate synthase large subunit (EC 2.2.1.6), at 5,963,258 to 5,964,991
_5175
Pf6N2E2_5176 - Acetolactate synthase small subunit (EC 2.2.1.6), at 5,964,994 to 5,965,485
_5176
Pf6N2E2_5177 - Ketol-acid reductoisomerase (EC 1.1.1.86), at 5,965,531 to 5,966,547
_5177
Pf6N2E2_5178 - CDP-diacylglycerol--serine O-phosphatidyltransferase (EC 2.7.8.8), at 5,966,693 to 5,967,538
_5178
Position (kb)
5965
5966
5967 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 5964.662 kb on + strand, within Pf6N2E2_5175 at 5964.663 kb on - strand, within Pf6N2E2_5175 at 5964.737 kb on + strand, within Pf6N2E2_5175 at 5964.738 kb on - strand, within Pf6N2E2_5175 at 5964.761 kb on + strand, within Pf6N2E2_5175 at 5964.761 kb on + strand, within Pf6N2E2_5175 at 5964.761 kb on + strand, within Pf6N2E2_5175 at 5964.761 kb on + strand, within Pf6N2E2_5175 at 5964.761 kb on + strand, within Pf6N2E2_5175 at 5964.762 kb on - strand, within Pf6N2E2_5175 at 5964.762 kb on - strand, within Pf6N2E2_5175 at 5964.803 kb on + strand, within Pf6N2E2_5175 at 5964.961 kb on + strand at 5964.961 kb on + strand at 5964.985 kb on + strand at 5964.985 kb on + strand at 5964.985 kb on + strand at 5964.986 kb on - strand at 5964.989 kb on + strand at 5964.989 kb on + strand at 5964.989 kb on + strand at 5964.989 kb on + strand at 5964.989 kb on + strand at 5964.990 kb on - strand at 5964.990 kb on - strand at 5964.990 kb on - strand at 5964.990 kb on - strand at 5965.005 kb on + strand at 5965.005 kb on + strand at 5965.006 kb on - strand at 5965.006 kb on - strand at 5965.006 kb on - strand at 5965.006 kb on - strand at 5965.052 kb on + strand, within Pf6N2E2_5176 at 5965.052 kb on + strand, within Pf6N2E2_5176 at 5965.052 kb on + strand, within Pf6N2E2_5176 at 5965.052 kb on + strand, within Pf6N2E2_5176 at 5965.052 kb on + strand, within Pf6N2E2_5176 at 5965.075 kb on + strand, within Pf6N2E2_5176 at 5965.075 kb on + strand, within Pf6N2E2_5176 at 5965.075 kb on + strand, within Pf6N2E2_5176 at 5965.076 kb on - strand, within Pf6N2E2_5176 at 5965.076 kb on - strand, within Pf6N2E2_5176 at 5965.076 kb on - strand, within Pf6N2E2_5176 at 5965.076 kb on - strand, within Pf6N2E2_5176 at 5965.076 kb on - strand, within Pf6N2E2_5176 at 5965.302 kb on + strand, within Pf6N2E2_5176 at 5965.303 kb on - strand, within Pf6N2E2_5176 at 5965.303 kb on - strand, within Pf6N2E2_5176 at 5965.303 kb on - strand, within Pf6N2E2_5176 at 5965.303 kb on - strand, within Pf6N2E2_5176 at 5965.305 kb on + strand, within Pf6N2E2_5176 at 5965.305 kb on + strand, within Pf6N2E2_5176 at 5965.312 kb on - strand, within Pf6N2E2_5176 at 5965.316 kb on - strand, within Pf6N2E2_5176 at 5965.316 kb on - strand, within Pf6N2E2_5176 at 5965.316 kb on - strand, within Pf6N2E2_5176 at 5965.351 kb on + strand, within Pf6N2E2_5176 at 5965.351 kb on + strand, within Pf6N2E2_5176 at 5965.351 kb on + strand, within Pf6N2E2_5176 at 5965.351 kb on + strand, within Pf6N2E2_5176 at 5965.351 kb on + strand, within Pf6N2E2_5176 at 5965.352 kb on - strand, within Pf6N2E2_5176 at 5965.352 kb on - strand, within Pf6N2E2_5176 at 5965.352 kb on - strand, within Pf6N2E2_5176 at 5965.353 kb on + strand, within Pf6N2E2_5176 at 5965.472 kb on + strand at 5965.472 kb on + strand at 5965.472 kb on + strand at 5965.472 kb on + strand at 5965.473 kb on - strand at 5965.473 kb on - strand at 5965.473 kb on - strand at 5965.483 kb on + strand at 5965.484 kb on - strand at 5965.484 kb on - strand at 5965.493 kb on + strand at 5965.543 kb on + strand at 5965.867 kb on + strand, within Pf6N2E2_5177 at 5965.867 kb on + strand, within Pf6N2E2_5177 at 5965.867 kb on + strand, within Pf6N2E2_5177 at 5965.867 kb on + strand, within Pf6N2E2_5177 at 5965.867 kb on + strand, within Pf6N2E2_5177 at 5965.868 kb on - strand, within Pf6N2E2_5177 at 5965.988 kb on - strand, within Pf6N2E2_5177 at 5965.988 kb on - strand, within Pf6N2E2_5177 at 5965.988 kb on - strand, within Pf6N2E2_5177 at 5966.030 kb on - strand, within Pf6N2E2_5177 at 5966.055 kb on + strand, within Pf6N2E2_5177 at 5966.182 kb on + strand, within Pf6N2E2_5177 at 5966.200 kb on + strand, within Pf6N2E2_5177 at 5966.200 kb on + strand, within Pf6N2E2_5177 at 5966.200 kb on + strand, within Pf6N2E2_5177 at 5966.200 kb on + strand, within Pf6N2E2_5177 at 5966.201 kb on - strand, within Pf6N2E2_5177 at 5966.245 kb on + strand, within Pf6N2E2_5177 at 5966.245 kb on + strand, within Pf6N2E2_5177 at 5966.245 kb on + strand, within Pf6N2E2_5177 at 5966.245 kb on + strand, within Pf6N2E2_5177 at 5966.245 kb on + strand, within Pf6N2E2_5177 at 5966.245 kb on + strand, within Pf6N2E2_5177 at 5966.245 kb on + strand, within Pf6N2E2_5177 at 5966.245 kb on + strand, within Pf6N2E2_5177 at 5966.246 kb on - strand, within Pf6N2E2_5177 at 5966.246 kb on - strand, within Pf6N2E2_5177 at 5966.246 kb on - strand, within Pf6N2E2_5177 at 5966.272 kb on + strand, within Pf6N2E2_5177 at 5966.272 kb on + strand, within Pf6N2E2_5177 at 5966.273 kb on - strand, within Pf6N2E2_5177 at 5966.273 kb on - strand, within Pf6N2E2_5177 at 5966.273 kb on - strand, within Pf6N2E2_5177 at 5966.305 kb on + strand, within Pf6N2E2_5177 at 5966.386 kb on + strand, within Pf6N2E2_5177 at 5966.545 kb on + strand at 5966.545 kb on + strand at 5966.545 kb on + strand at 5966.546 kb on - strand at 5966.546 kb on - strand at 5966.546 kb on - strand at 5966.546 kb on - strand at 5966.546 kb on - strand at 5966.546 kb on - strand at 5966.615 kb on + strand at 5966.616 kb on - strand at 5966.685 kb on + strand at 5966.686 kb on - strand at 5966.686 kb on - strand at 5966.818 kb on - strand, within Pf6N2E2_5178 at 5967.140 kb on - strand, within Pf6N2E2_5178 at 5967.143 kb on + strand, within Pf6N2E2_5178 at 5967.144 kb on - strand, within Pf6N2E2_5178 at 5967.279 kb on - strand, within Pf6N2E2_5178 at 5967.362 kb on + strand, within Pf6N2E2_5178 at 5967.512 kb on + strand at 5967.513 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 5,964,662 + Pf6N2E2_5175 0.81 -1.4 5,964,663 - Pf6N2E2_5175 0.81 +1.4 5,964,737 + Pf6N2E2_5175 0.85 +1.4 5,964,738 - Pf6N2E2_5175 0.85 +1.4 5,964,761 + Pf6N2E2_5175 0.87 -2.1 5,964,761 + Pf6N2E2_5175 0.87 +1.4 5,964,761 + Pf6N2E2_5175 0.87 +1.4 5,964,761 + Pf6N2E2_5175 0.87 -2.1 5,964,761 + Pf6N2E2_5175 0.87 +1.4 5,964,762 - Pf6N2E2_5175 0.87 -0.6 5,964,762 - Pf6N2E2_5175 0.87 -2.3 5,964,803 + Pf6N2E2_5175 0.89 -0.2 5,964,961 + -0.6 5,964,961 + -2.8 5,964,985 + -3.1 5,964,985 + -0.9 5,964,985 + -2.1 5,964,986 - -0.9 5,964,989 + +1.4 5,964,989 + -3.2 5,964,989 + -2.1 5,964,989 + -2.9 5,964,989 + -1.2 5,964,990 - +0.4 5,964,990 - -1.5 5,964,990 - +1.4 5,964,990 - -1.6 5,965,005 + -1.4 5,965,005 + -2.8 5,965,006 - -1.6 5,965,006 - +1.4 5,965,006 - +0.4 5,965,006 - +0.4 5,965,052 + Pf6N2E2_5176 0.12 +1.4 5,965,052 + Pf6N2E2_5176 0.12 +0.4 5,965,052 + Pf6N2E2_5176 0.12 -0.6 5,965,052 + Pf6N2E2_5176 0.12 -1.9 5,965,052 + Pf6N2E2_5176 0.12 +0.4 5,965,075 + Pf6N2E2_5176 0.16 -1.9 5,965,075 + Pf6N2E2_5176 0.16 -1.2 5,965,075 + Pf6N2E2_5176 0.16 -0.2 5,965,076 - Pf6N2E2_5176 0.17 +0.4 5,965,076 - Pf6N2E2_5176 0.17 -0.9 5,965,076 - Pf6N2E2_5176 0.17 -0.9 5,965,076 - Pf6N2E2_5176 0.17 -1.8 5,965,076 - Pf6N2E2_5176 0.17 -1.6 5,965,302 + Pf6N2E2_5176 0.63 +0.4 5,965,303 - Pf6N2E2_5176 0.63 -0.9 5,965,303 - Pf6N2E2_5176 0.63 -2.3 5,965,303 - Pf6N2E2_5176 0.63 -2.1 5,965,303 - Pf6N2E2_5176 0.63 -0.2 5,965,305 + Pf6N2E2_5176 0.63 -1.3 5,965,305 + Pf6N2E2_5176 0.63 +0.4 5,965,312 - Pf6N2E2_5176 0.65 -2.3 5,965,316 - Pf6N2E2_5176 0.65 -1.2 5,965,316 - Pf6N2E2_5176 0.65 -3.3 5,965,316 - Pf6N2E2_5176 0.65 -0.2 5,965,351 + Pf6N2E2_5176 0.73 -2.3 5,965,351 + Pf6N2E2_5176 0.73 -0.6 5,965,351 + Pf6N2E2_5176 0.73 -2.2 5,965,351 + Pf6N2E2_5176 0.73 -2.9 5,965,351 + Pf6N2E2_5176 0.73 +1.4 5,965,352 - Pf6N2E2_5176 0.73 -1.8 5,965,352 - Pf6N2E2_5176 0.73 -0.6 5,965,352 - Pf6N2E2_5176 0.73 -0.6 5,965,353 + Pf6N2E2_5176 0.73 -1.8 5,965,472 + +0.8 5,965,472 + -2.9 5,965,472 + +1.4 5,965,472 + -1.8 5,965,473 - -3.8 5,965,473 - -3.0 5,965,473 - -4.3 5,965,483 + -1.8 5,965,484 - +1.4 5,965,484 - -4.0 5,965,493 + -3.0 5,965,543 + -2.1 5,965,867 + Pf6N2E2_5177 0.33 -0.6 5,965,867 + Pf6N2E2_5177 0.33 -2.6 5,965,867 + Pf6N2E2_5177 0.33 -3.8 5,965,867 + Pf6N2E2_5177 0.33 -3.0 5,965,867 + Pf6N2E2_5177 0.33 -3.7 5,965,868 - Pf6N2E2_5177 0.33 -0.8 5,965,988 - Pf6N2E2_5177 0.45 -1.4 5,965,988 - Pf6N2E2_5177 0.45 -2.2 5,965,988 - Pf6N2E2_5177 0.45 -1.2 5,966,030 - Pf6N2E2_5177 0.49 -3.5 5,966,055 + Pf6N2E2_5177 0.52 -2.7 5,966,182 + Pf6N2E2_5177 0.64 -2.5 5,966,200 + Pf6N2E2_5177 0.66 -1.1 5,966,200 + Pf6N2E2_5177 0.66 -3.2 5,966,200 + Pf6N2E2_5177 0.66 -3.6 5,966,200 + Pf6N2E2_5177 0.66 -2.8 5,966,201 - Pf6N2E2_5177 0.66 -4.5 5,966,245 + Pf6N2E2_5177 0.70 -3.5 5,966,245 + Pf6N2E2_5177 0.70 -2.4 5,966,245 + Pf6N2E2_5177 0.70 -3.0 5,966,245 + Pf6N2E2_5177 0.70 -4.1 5,966,245 + Pf6N2E2_5177 0.70 -3.0 5,966,245 + Pf6N2E2_5177 0.70 -2.5 5,966,245 + Pf6N2E2_5177 0.70 -1.6 5,966,245 + Pf6N2E2_5177 0.70 -4.8 5,966,246 - Pf6N2E2_5177 0.70 +0.4 5,966,246 - Pf6N2E2_5177 0.70 -3.1 5,966,246 - Pf6N2E2_5177 0.70 -4.0 5,966,272 + Pf6N2E2_5177 0.73 +0.5 5,966,272 + Pf6N2E2_5177 0.73 -2.8 5,966,273 - Pf6N2E2_5177 0.73 +0.1 5,966,273 - Pf6N2E2_5177 0.73 -1.6 5,966,273 - Pf6N2E2_5177 0.73 +0.4 5,966,305 + Pf6N2E2_5177 0.76 -2.7 5,966,386 + Pf6N2E2_5177 0.84 -2.3 5,966,545 + -1.4 5,966,545 + -0.6 5,966,545 + -1.3 5,966,546 - -1.6 5,966,546 - -1.0 5,966,546 - -2.3 5,966,546 - -1.6 5,966,546 - -1.5 5,966,546 - -0.9 5,966,615 + +0.0 5,966,616 - -0.4 5,966,685 + +0.4 5,966,686 - -0.5 5,966,686 - -2.5 5,966,818 - Pf6N2E2_5178 0.15 -0.2 5,967,140 - Pf6N2E2_5178 0.53 +0.5 5,967,143 + Pf6N2E2_5178 0.53 +0.6 5,967,144 - Pf6N2E2_5178 0.53 +0.7 5,967,279 - Pf6N2E2_5178 0.69 -0.6 5,967,362 + Pf6N2E2_5178 0.79 +0.1 5,967,512 + +1.4 5,967,513 - -0.2
Or see this region's nucleotide sequence