Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5171 and Pf6N2E2_5172 are separated by 16 nucleotides Pf6N2E2_5172 and Pf6N2E2_5173 overlap by 1 nucleotides Pf6N2E2_5173 and Pf6N2E2_5174 are separated by 6 nucleotides Pf6N2E2_5174 and Pf6N2E2_5175 are separated by 398 nucleotides
Pf6N2E2_5171 - Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-), at 5,959,155 to 5,961,371
_5171
Pf6N2E2_5172 - FOG: TPR repeat, at 5,961,388 to 5,962,107
_5172
Pf6N2E2_5173 - Hypothetical protein YqcC (clustered with tRNA pseudouridine synthase C), at 5,962,107 to 5,962,436
_5173
Pf6N2E2_5174 - Glycosyltransferase, at 5,962,443 to 5,962,859
_5174
Pf6N2E2_5175 - Acetolactate synthase large subunit (EC 2.2.1.6), at 5,963,258 to 5,964,991
_5175
Position (kb)
5962
5963 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 5961.276 kb on + strand at 5961.276 kb on + strand at 5961.276 kb on + strand at 5961.277 kb on - strand at 5961.277 kb on - strand at 5961.278 kb on + strand at 5961.279 kb on - strand at 5961.369 kb on + strand at 5961.369 kb on + strand at 5961.369 kb on + strand at 5961.369 kb on + strand at 5961.369 kb on + strand at 5961.370 kb on - strand at 5961.370 kb on - strand at 5961.444 kb on + strand at 5961.444 kb on + strand at 5961.445 kb on - strand at 5961.449 kb on + strand at 5961.449 kb on + strand at 5961.449 kb on + strand at 5961.715 kb on + strand, within Pf6N2E2_5172 at 5961.715 kb on + strand, within Pf6N2E2_5172 at 5961.715 kb on + strand, within Pf6N2E2_5172 at 5961.716 kb on - strand, within Pf6N2E2_5172 at 5961.716 kb on - strand, within Pf6N2E2_5172 at 5961.716 kb on - strand, within Pf6N2E2_5172 at 5962.293 kb on - strand, within Pf6N2E2_5173 at 5962.356 kb on + strand, within Pf6N2E2_5173 at 5962.356 kb on + strand, within Pf6N2E2_5173 at 5962.357 kb on - strand, within Pf6N2E2_5173 at 5962.357 kb on - strand, within Pf6N2E2_5173 at 5962.357 kb on - strand, within Pf6N2E2_5173 at 5962.357 kb on - strand, within Pf6N2E2_5173 at 5962.542 kb on + strand, within Pf6N2E2_5174 at 5962.543 kb on - strand, within Pf6N2E2_5174 at 5962.602 kb on + strand, within Pf6N2E2_5174 at 5962.603 kb on - strand, within Pf6N2E2_5174 at 5962.603 kb on - strand, within Pf6N2E2_5174 at 5962.795 kb on + strand, within Pf6N2E2_5174 at 5962.916 kb on + strand at 5962.946 kb on + strand at 5962.947 kb on - strand at 5962.947 kb on - strand at 5962.964 kb on + strand at 5962.964 kb on + strand at 5962.964 kb on + strand at 5962.965 kb on - strand at 5963.015 kb on - strand at 5963.078 kb on + strand at 5963.079 kb on - strand at 5963.079 kb on - strand at 5963.079 kb on - strand at 5963.142 kb on + strand at 5963.142 kb on + strand at 5963.143 kb on - strand at 5963.165 kb on + strand at 5963.165 kb on + strand at 5963.165 kb on + strand at 5963.166 kb on - strand at 5963.203 kb on + strand at 5963.203 kb on + strand at 5963.203 kb on + strand at 5963.203 kb on + strand at 5963.203 kb on + strand at 5963.203 kb on + strand at 5963.203 kb on + strand at 5963.203 kb on + strand at 5963.204 kb on - strand at 5963.209 kb on + strand at 5963.209 kb on + strand at 5963.209 kb on + strand at 5963.209 kb on + strand at 5963.209 kb on + strand at 5963.209 kb on + strand at 5963.209 kb on + strand at 5963.209 kb on + strand at 5963.210 kb on - strand at 5963.210 kb on - strand at 5963.212 kb on + strand at 5963.212 kb on + strand at 5963.212 kb on + strand at 5963.212 kb on + strand at 5963.213 kb on - strand at 5963.213 kb on - strand at 5963.213 kb on - strand at 5963.213 kb on - strand at 5963.278 kb on - strand at 5963.278 kb on - strand at 5963.327 kb on + strand at 5963.327 kb on + strand at 5963.327 kb on + strand at 5963.327 kb on + strand at 5963.327 kb on + strand at 5963.327 kb on + strand at 5963.327 kb on + strand at 5963.328 kb on - strand at 5963.328 kb on - strand at 5963.328 kb on - strand at 5963.328 kb on - strand at 5963.329 kb on + strand at 5963.329 kb on + strand at 5963.329 kb on + strand at 5963.329 kb on + strand at 5963.330 kb on - strand at 5963.333 kb on + strand at 5963.366 kb on + strand at 5963.366 kb on + strand at 5963.366 kb on + strand at 5963.367 kb on - strand at 5963.367 kb on - strand at 5963.367 kb on - strand at 5963.367 kb on - strand at 5963.367 kb on - strand at 5963.434 kb on + strand, within Pf6N2E2_5175 at 5963.434 kb on + strand, within Pf6N2E2_5175 at 5963.434 kb on + strand, within Pf6N2E2_5175
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 5,961,276 + -1.1 5,961,276 + -0.4 5,961,276 + -0.2 5,961,277 - -0.4 5,961,277 - -0.5 5,961,278 + -0.8 5,961,279 - +0.6 5,961,369 + -2.8 5,961,369 + -1.2 5,961,369 + -1.2 5,961,369 + +0.7 5,961,369 + -0.5 5,961,370 - -1.1 5,961,370 - -2.6 5,961,444 + -0.4 5,961,444 + -0.2 5,961,445 - -1.3 5,961,449 + -0.3 5,961,449 + -0.1 5,961,449 + -1.1 5,961,715 + Pf6N2E2_5172 0.45 +1.4 5,961,715 + Pf6N2E2_5172 0.45 -0.5 5,961,715 + Pf6N2E2_5172 0.45 -1.0 5,961,716 - Pf6N2E2_5172 0.46 +0.1 5,961,716 - Pf6N2E2_5172 0.46 -0.7 5,961,716 - Pf6N2E2_5172 0.46 +1.4 5,962,293 - Pf6N2E2_5173 0.56 -2.0 5,962,356 + Pf6N2E2_5173 0.75 -1.4 5,962,356 + Pf6N2E2_5173 0.75 -0.4 5,962,357 - Pf6N2E2_5173 0.76 -0.5 5,962,357 - Pf6N2E2_5173 0.76 -1.4 5,962,357 - Pf6N2E2_5173 0.76 -0.9 5,962,357 - Pf6N2E2_5173 0.76 +1.4 5,962,542 + Pf6N2E2_5174 0.24 +0.2 5,962,543 - Pf6N2E2_5174 0.24 -0.8 5,962,602 + Pf6N2E2_5174 0.38 +0.9 5,962,603 - Pf6N2E2_5174 0.38 -1.8 5,962,603 - Pf6N2E2_5174 0.38 -0.2 5,962,795 + Pf6N2E2_5174 0.84 +0.7 5,962,916 + -1.2 5,962,946 + +0.8 5,962,947 - -4.1 5,962,947 - -2.5 5,962,964 + -1.9 5,962,964 + +1.4 5,962,964 + -0.2 5,962,965 - -4.2 5,963,015 - +1.4 5,963,078 + -3.0 5,963,079 - -1.2 5,963,079 - -2.3 5,963,079 - +1.4 5,963,142 + +1.1 5,963,142 + +2.4 5,963,143 - -2.2 5,963,165 + +0.8 5,963,165 + +1.4 5,963,165 + +1.6 5,963,166 - -0.2 5,963,203 + +1.4 5,963,203 + +0.3 5,963,203 + +1.7 5,963,203 + +1.1 5,963,203 + -0.1 5,963,203 + +1.9 5,963,203 + +1.9 5,963,203 + -0.0 5,963,204 - -1.4 5,963,209 + +1.1 5,963,209 + +1.5 5,963,209 + +1.7 5,963,209 + +0.4 5,963,209 + +1.3 5,963,209 + +1.4 5,963,209 + +2.3 5,963,209 + +0.4 5,963,210 - -3.5 5,963,210 - -1.2 5,963,212 + +1.1 5,963,212 + -0.2 5,963,212 + +1.0 5,963,212 + -1.8 5,963,213 - -1.6 5,963,213 - -1.2 5,963,213 - -1.6 5,963,213 - -2.3 5,963,278 - -1.2 5,963,278 - +1.4 5,963,327 + -1.4 5,963,327 + -0.6 5,963,327 + +1.4 5,963,327 + -0.6 5,963,327 + +0.4 5,963,327 + -0.2 5,963,327 + -0.2 5,963,328 - +0.2 5,963,328 - -2.8 5,963,328 - -1.4 5,963,328 - +1.4 5,963,329 + -0.2 5,963,329 + -1.1 5,963,329 + -0.2 5,963,329 + +0.4 5,963,330 - -3.2 5,963,333 + +0.4 5,963,366 + -0.6 5,963,366 + -0.9 5,963,366 + -0.2 5,963,367 - -1.9 5,963,367 - -2.1 5,963,367 - -0.9 5,963,367 - -0.2 5,963,367 - -1.4 5,963,434 + Pf6N2E2_5175 0.10 -1.2 5,963,434 + Pf6N2E2_5175 0.10 -1.4 5,963,434 + Pf6N2E2_5175 0.10 -0.6
Or see this region's nucleotide sequence