Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5114 and Pf6N2E2_5115 overlap by 4 nucleotides Pf6N2E2_5115 and Pf6N2E2_5116 overlap by 4 nucleotides Pf6N2E2_5116 and Pf6N2E2_5117 are separated by 20 nucleotides Pf6N2E2_5117 and Pf6N2E2_5118 overlap by 14 nucleotides
Pf6N2E2_5114 - type 4 fimbrial biogenesis protein FimU, at 5,897,598 to 5,898,071
_5114
Pf6N2E2_5115 - Type IV fimbrial biogenesis protein PilV, at 5,898,068 to 5,898,529
_5115
Pf6N2E2_5116 - Type IV fimbrial biogenesis protein PilW, at 5,898,526 to 5,899,242
_5116
Pf6N2E2_5117 - Type IV fimbrial biogenesis protein PilX, at 5,899,263 to 5,899,733
_5117
Pf6N2E2_5118 - Type IV fimbrial biogenesis protein PilY1, at 5,899,720 to 5,902,818
_5118
Position (kb)
5898
5899
5900 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5897.528 kb on - strand at 5897.528 kb on - strand at 5897.554 kb on + strand at 5897.554 kb on + strand at 5897.562 kb on - strand at 5897.562 kb on - strand at 5897.562 kb on - strand at 5897.571 kb on + strand at 5897.571 kb on + strand at 5897.572 kb on - strand at 5897.572 kb on - strand at 5897.572 kb on - strand at 5897.572 kb on - strand at 5897.572 kb on - strand at 5897.572 kb on - strand at 5897.573 kb on + strand at 5897.574 kb on - strand at 5897.574 kb on - strand at 5897.574 kb on - strand at 5897.610 kb on - strand at 5897.610 kb on - strand at 5897.610 kb on - strand at 5897.669 kb on + strand, within Pf6N2E2_5114 at 5897.670 kb on - strand, within Pf6N2E2_5114 at 5897.670 kb on - strand, within Pf6N2E2_5114 at 5897.844 kb on + strand, within Pf6N2E2_5114 at 5897.844 kb on + strand, within Pf6N2E2_5114 at 5897.844 kb on + strand, within Pf6N2E2_5114 at 5897.845 kb on - strand, within Pf6N2E2_5114 at 5897.845 kb on - strand, within Pf6N2E2_5114 at 5897.845 kb on - strand, within Pf6N2E2_5114 at 5897.845 kb on - strand, within Pf6N2E2_5114 at 5897.852 kb on + strand, within Pf6N2E2_5114 at 5897.872 kb on + strand, within Pf6N2E2_5114 at 5897.872 kb on + strand, within Pf6N2E2_5114 at 5897.872 kb on + strand, within Pf6N2E2_5114 at 5897.872 kb on + strand, within Pf6N2E2_5114 at 5897.937 kb on - strand, within Pf6N2E2_5114 at 5897.994 kb on + strand, within Pf6N2E2_5114 at 5897.994 kb on + strand, within Pf6N2E2_5114 at 5897.995 kb on - strand, within Pf6N2E2_5114 at 5897.995 kb on - strand, within Pf6N2E2_5114 at 5897.995 kb on - strand, within Pf6N2E2_5114 at 5897.995 kb on - strand, within Pf6N2E2_5114 at 5897.995 kb on - strand, within Pf6N2E2_5114 at 5897.995 kb on - strand, within Pf6N2E2_5114 at 5897.998 kb on + strand, within Pf6N2E2_5114 at 5897.998 kb on + strand, within Pf6N2E2_5114 at 5897.998 kb on + strand, within Pf6N2E2_5114 at 5897.998 kb on + strand, within Pf6N2E2_5114 at 5897.998 kb on + strand, within Pf6N2E2_5114 at 5897.998 kb on + strand, within Pf6N2E2_5114 at 5897.999 kb on - strand, within Pf6N2E2_5114 at 5897.999 kb on - strand, within Pf6N2E2_5114 at 5897.999 kb on - strand, within Pf6N2E2_5114 at 5898.050 kb on - strand at 5898.050 kb on - strand at 5898.188 kb on + strand, within Pf6N2E2_5115 at 5898.188 kb on + strand, within Pf6N2E2_5115 at 5898.189 kb on - strand, within Pf6N2E2_5115 at 5898.189 kb on - strand, within Pf6N2E2_5115 at 5898.234 kb on - strand, within Pf6N2E2_5115 at 5898.275 kb on + strand, within Pf6N2E2_5115 at 5898.275 kb on + strand, within Pf6N2E2_5115 at 5898.275 kb on + strand, within Pf6N2E2_5115 at 5898.276 kb on - strand, within Pf6N2E2_5115 at 5898.276 kb on - strand, within Pf6N2E2_5115 at 5898.408 kb on - strand, within Pf6N2E2_5115 at 5898.633 kb on + strand, within Pf6N2E2_5116 at 5898.634 kb on - strand, within Pf6N2E2_5116 at 5898.637 kb on + strand, within Pf6N2E2_5116 at 5898.637 kb on + strand, within Pf6N2E2_5116 at 5898.637 kb on + strand, within Pf6N2E2_5116 at 5898.637 kb on + strand, within Pf6N2E2_5116 at 5898.638 kb on - strand, within Pf6N2E2_5116 at 5898.638 kb on - strand, within Pf6N2E2_5116 at 5898.970 kb on + strand, within Pf6N2E2_5116 at 5898.970 kb on + strand, within Pf6N2E2_5116 at 5899.040 kb on + strand, within Pf6N2E2_5116 at 5899.112 kb on - strand, within Pf6N2E2_5116 at 5899.211 kb on + strand at 5899.211 kb on + strand at 5899.211 kb on + strand at 5899.212 kb on - strand at 5899.212 kb on - strand at 5899.212 kb on - strand at 5899.212 kb on - strand at 5899.212 kb on - strand at 5899.238 kb on + strand at 5899.238 kb on + strand at 5899.238 kb on + strand at 5899.238 kb on + strand at 5899.238 kb on + strand at 5899.238 kb on + strand at 5899.238 kb on + strand at 5899.239 kb on - strand at 5899.239 kb on - strand at 5899.239 kb on - strand at 5899.239 kb on - strand at 5899.239 kb on - strand at 5899.239 kb on - strand at 5899.467 kb on + strand, within Pf6N2E2_5117 at 5899.467 kb on + strand, within Pf6N2E2_5117 at 5899.467 kb on + strand, within Pf6N2E2_5117 at 5899.468 kb on - strand, within Pf6N2E2_5117 at 5899.468 kb on - strand, within Pf6N2E2_5117 at 5899.468 kb on - strand, within Pf6N2E2_5117 at 5899.468 kb on - strand, within Pf6N2E2_5117 at 5899.468 kb on - strand, within Pf6N2E2_5117 at 5899.468 kb on - strand, within Pf6N2E2_5117 at 5899.468 kb on - strand, within Pf6N2E2_5117 at 5899.484 kb on + strand, within Pf6N2E2_5117 at 5899.485 kb on - strand, within Pf6N2E2_5117 at 5899.590 kb on + strand, within Pf6N2E2_5117 at 5899.590 kb on + strand, within Pf6N2E2_5117 at 5899.590 kb on + strand, within Pf6N2E2_5117 at 5899.610 kb on + strand, within Pf6N2E2_5117 at 5899.611 kb on - strand, within Pf6N2E2_5117 at 5899.611 kb on - strand, within Pf6N2E2_5117 at 5899.611 kb on - strand, within Pf6N2E2_5117 at 5899.613 kb on + strand, within Pf6N2E2_5117 at 5899.613 kb on + strand, within Pf6N2E2_5117 at 5899.613 kb on + strand, within Pf6N2E2_5117 at 5899.614 kb on - strand, within Pf6N2E2_5117 at 5899.614 kb on - strand, within Pf6N2E2_5117 at 5899.614 kb on - strand, within Pf6N2E2_5117 at 5899.614 kb on - strand, within Pf6N2E2_5117 at 5899.643 kb on + strand, within Pf6N2E2_5117 at 5899.644 kb on - strand, within Pf6N2E2_5117 at 5899.644 kb on - strand, within Pf6N2E2_5117 at 5899.644 kb on - strand, within Pf6N2E2_5117 at 5899.660 kb on - strand, within Pf6N2E2_5117 at 5899.692 kb on - strand at 5899.719 kb on + strand at 5899.720 kb on - strand at 5899.720 kb on - strand at 5899.728 kb on + strand at 5899.728 kb on + strand at 5899.728 kb on + strand at 5899.728 kb on + strand at 5899.729 kb on - strand at 5899.729 kb on - strand at 5899.729 kb on - strand at 5899.729 kb on - strand at 5899.731 kb on + strand at 5899.731 kb on + strand at 5899.731 kb on + strand at 5899.731 kb on + strand at 5899.731 kb on + strand at 5899.732 kb on - strand at 5899.732 kb on - strand at 5899.732 kb on - strand at 5899.783 kb on + strand at 5899.783 kb on + strand at 5899.784 kb on - strand at 5899.802 kb on - strand at 5899.802 kb on - strand at 5899.899 kb on + strand at 5899.899 kb on + strand at 5899.899 kb on + strand at 5899.899 kb on + strand at 5899.899 kb on + strand at 5899.899 kb on + strand at 5899.899 kb on + strand at 5899.899 kb on + strand at 5899.899 kb on + strand at 5899.900 kb on - strand at 5899.900 kb on - strand at 5899.900 kb on - strand at 5899.900 kb on - strand at 5899.900 kb on - strand at 5899.900 kb on - strand at 5899.945 kb on + strand at 5899.945 kb on + strand at 5899.945 kb on + strand at 5899.945 kb on + strand at 5899.945 kb on + strand at 5899.945 kb on + strand at 5899.946 kb on - strand at 5899.946 kb on - strand at 5899.946 kb on - strand at 5899.946 kb on - strand at 5899.946 kb on - strand at 5899.946 kb on - strand at 5899.946 kb on - strand at 5899.946 kb on - strand at 5899.963 kb on - strand at 5899.981 kb on + strand at 5899.981 kb on + strand at 5899.981 kb on + strand at 5899.981 kb on + strand at 5899.981 kb on + strand at 5899.981 kb on + strand at 5899.981 kb on + strand at 5899.982 kb on - strand at 5900.018 kb on + strand at 5900.128 kb on + strand, within Pf6N2E2_5118 at 5900.130 kb on + strand, within Pf6N2E2_5118 at 5900.130 kb on + strand, within Pf6N2E2_5118 at 5900.130 kb on + strand, within Pf6N2E2_5118 at 5900.131 kb on - strand, within Pf6N2E2_5118 at 5900.137 kb on - strand, within Pf6N2E2_5118 at 5900.140 kb on + strand, within Pf6N2E2_5118 at 5900.140 kb on + strand, within Pf6N2E2_5118 at 5900.140 kb on + strand, within Pf6N2E2_5118 at 5900.141 kb on - strand, within Pf6N2E2_5118 at 5900.141 kb on - strand, within Pf6N2E2_5118 at 5900.141 kb on - strand, within Pf6N2E2_5118 at 5900.149 kb on + strand, within Pf6N2E2_5118 at 5900.150 kb on - strand, within Pf6N2E2_5118
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 5,897,528 - +0.3 5,897,528 - -0.1 5,897,554 + +1.4 5,897,554 + -0.6 5,897,562 - +1.8 5,897,562 - -0.0 5,897,562 - -0.0 5,897,571 + -0.5 5,897,571 + -0.8 5,897,572 - +0.0 5,897,572 - +0.1 5,897,572 - -0.3 5,897,572 - +0.4 5,897,572 - +0.1 5,897,572 - -1.2 5,897,573 + +0.8 5,897,574 - -0.2 5,897,574 - -0.2 5,897,574 - -0.6 5,897,610 - +0.5 5,897,610 - -0.4 5,897,610 - +0.8 5,897,669 + Pf6N2E2_5114 0.15 -0.8 5,897,670 - Pf6N2E2_5114 0.15 -0.3 5,897,670 - Pf6N2E2_5114 0.15 +0.6 5,897,844 + Pf6N2E2_5114 0.52 +1.4 5,897,844 + Pf6N2E2_5114 0.52 +0.4 5,897,844 + Pf6N2E2_5114 0.52 +0.2 5,897,845 - Pf6N2E2_5114 0.52 +0.3 5,897,845 - Pf6N2E2_5114 0.52 +1.6 5,897,845 - Pf6N2E2_5114 0.52 +0.1 5,897,845 - Pf6N2E2_5114 0.52 +0.1 5,897,852 + Pf6N2E2_5114 0.54 -0.9 5,897,872 + Pf6N2E2_5114 0.58 -0.4 5,897,872 + Pf6N2E2_5114 0.58 +0.7 5,897,872 + Pf6N2E2_5114 0.58 +0.4 5,897,872 + Pf6N2E2_5114 0.58 -1.2 5,897,937 - Pf6N2E2_5114 0.72 +0.2 5,897,994 + Pf6N2E2_5114 0.84 -0.6 5,897,994 + Pf6N2E2_5114 0.84 +1.1 5,897,995 - Pf6N2E2_5114 0.84 -0.1 5,897,995 - Pf6N2E2_5114 0.84 -0.4 5,897,995 - Pf6N2E2_5114 0.84 +1.4 5,897,995 - Pf6N2E2_5114 0.84 -0.1 5,897,995 - Pf6N2E2_5114 0.84 -0.9 5,897,995 - Pf6N2E2_5114 0.84 -0.6 5,897,998 + Pf6N2E2_5114 0.84 -1.1 5,897,998 + Pf6N2E2_5114 0.84 -1.2 5,897,998 + Pf6N2E2_5114 0.84 -0.5 5,897,998 + Pf6N2E2_5114 0.84 +0.5 5,897,998 + Pf6N2E2_5114 0.84 +0.5 5,897,998 + Pf6N2E2_5114 0.84 -0.0 5,897,999 - Pf6N2E2_5114 0.85 -0.2 5,897,999 - Pf6N2E2_5114 0.85 -0.2 5,897,999 - Pf6N2E2_5114 0.85 -0.1 5,898,050 - +0.6 5,898,050 - -0.0 5,898,188 + Pf6N2E2_5115 0.26 -0.7 5,898,188 + Pf6N2E2_5115 0.26 -0.4 5,898,189 - Pf6N2E2_5115 0.26 -0.5 5,898,189 - Pf6N2E2_5115 0.26 -0.3 5,898,234 - Pf6N2E2_5115 0.36 -0.2 5,898,275 + Pf6N2E2_5115 0.45 +0.4 5,898,275 + Pf6N2E2_5115 0.45 +0.2 5,898,275 + Pf6N2E2_5115 0.45 +1.2 5,898,276 - Pf6N2E2_5115 0.45 -0.7 5,898,276 - Pf6N2E2_5115 0.45 -0.3 5,898,408 - Pf6N2E2_5115 0.74 -0.2 5,898,633 + Pf6N2E2_5116 0.15 +0.7 5,898,634 - Pf6N2E2_5116 0.15 +0.5 5,898,637 + Pf6N2E2_5116 0.15 -0.8 5,898,637 + Pf6N2E2_5116 0.15 +0.5 5,898,637 + Pf6N2E2_5116 0.15 +0.6 5,898,637 + Pf6N2E2_5116 0.15 +1.1 5,898,638 - Pf6N2E2_5116 0.16 +0.5 5,898,638 - Pf6N2E2_5116 0.16 -0.9 5,898,970 + Pf6N2E2_5116 0.62 +1.4 5,898,970 + Pf6N2E2_5116 0.62 -1.1 5,899,040 + Pf6N2E2_5116 0.72 +1.1 5,899,112 - Pf6N2E2_5116 0.82 +0.5 5,899,211 + +0.4 5,899,211 + +0.5 5,899,211 + +1.4 5,899,212 - -0.4 5,899,212 - -0.4 5,899,212 - +0.7 5,899,212 - +0.0 5,899,212 - +0.2 5,899,238 + +0.6 5,899,238 + -0.8 5,899,238 + +0.6 5,899,238 + +1.2 5,899,238 + +1.7 5,899,238 + -0.3 5,899,238 + +0.2 5,899,239 - -0.4 5,899,239 - +0.6 5,899,239 - -0.6 5,899,239 - -0.2 5,899,239 - +0.6 5,899,239 - +0.1 5,899,467 + Pf6N2E2_5117 0.43 -1.7 5,899,467 + Pf6N2E2_5117 0.43 -0.4 5,899,467 + Pf6N2E2_5117 0.43 -0.1 5,899,468 - Pf6N2E2_5117 0.44 +0.6 5,899,468 - Pf6N2E2_5117 0.44 +0.1 5,899,468 - Pf6N2E2_5117 0.44 +0.1 5,899,468 - Pf6N2E2_5117 0.44 +0.2 5,899,468 - Pf6N2E2_5117 0.44 -0.4 5,899,468 - Pf6N2E2_5117 0.44 -1.0 5,899,468 - Pf6N2E2_5117 0.44 -0.2 5,899,484 + Pf6N2E2_5117 0.47 -0.1 5,899,485 - Pf6N2E2_5117 0.47 +1.1 5,899,590 + Pf6N2E2_5117 0.69 -0.5 5,899,590 + Pf6N2E2_5117 0.69 +0.1 5,899,590 + Pf6N2E2_5117 0.69 +0.0 5,899,610 + Pf6N2E2_5117 0.74 -0.5 5,899,611 - Pf6N2E2_5117 0.74 -0.6 5,899,611 - Pf6N2E2_5117 0.74 -0.3 5,899,611 - Pf6N2E2_5117 0.74 -1.2 5,899,613 + Pf6N2E2_5117 0.74 +1.4 5,899,613 + Pf6N2E2_5117 0.74 -0.2 5,899,613 + Pf6N2E2_5117 0.74 -0.0 5,899,614 - Pf6N2E2_5117 0.75 +0.2 5,899,614 - Pf6N2E2_5117 0.75 +0.3 5,899,614 - Pf6N2E2_5117 0.75 -0.3 5,899,614 - Pf6N2E2_5117 0.75 +0.8 5,899,643 + Pf6N2E2_5117 0.81 +0.3 5,899,644 - Pf6N2E2_5117 0.81 +0.6 5,899,644 - Pf6N2E2_5117 0.81 -0.3 5,899,644 - Pf6N2E2_5117 0.81 +0.0 5,899,660 - Pf6N2E2_5117 0.84 -0.7 5,899,692 - -1.6 5,899,719 + -0.2 5,899,720 - +0.9 5,899,720 - +0.7 5,899,728 + +1.6 5,899,728 + -0.6 5,899,728 + +1.1 5,899,728 + +1.4 5,899,729 - -1.6 5,899,729 - -0.9 5,899,729 - +0.0 5,899,729 - -0.0 5,899,731 + +0.5 5,899,731 + +0.2 5,899,731 + -0.2 5,899,731 + -1.9 5,899,731 + -0.1 5,899,732 - +0.0 5,899,732 - +0.0 5,899,732 - -0.8 5,899,783 + -0.7 5,899,783 + -0.2 5,899,784 - +0.4 5,899,802 - +0.7 5,899,802 - +0.4 5,899,899 + +0.2 5,899,899 + -0.4 5,899,899 + -0.3 5,899,899 + +0.8 5,899,899 + -0.4 5,899,899 + -0.0 5,899,899 + +0.4 5,899,899 + +0.7 5,899,899 + -0.9 5,899,900 - -0.6 5,899,900 - +0.8 5,899,900 - +1.4 5,899,900 - -0.0 5,899,900 - +0.1 5,899,900 - +0.0 5,899,945 + -1.7 5,899,945 + -2.7 5,899,945 + -0.2 5,899,945 + -0.9 5,899,945 + +3.0 5,899,945 + -1.0 5,899,946 - -0.2 5,899,946 - -0.1 5,899,946 - -0.2 5,899,946 - +0.4 5,899,946 - -0.1 5,899,946 - +0.3 5,899,946 - -0.4 5,899,946 - +0.4 5,899,963 - +0.2 5,899,981 + -0.1 5,899,981 + +0.4 5,899,981 + -0.4 5,899,981 + +0.9 5,899,981 + -0.4 5,899,981 + +0.1 5,899,981 + -1.8 5,899,982 - +0.4 5,900,018 + +0.7 5,900,128 + Pf6N2E2_5118 0.13 +1.2 5,900,130 + Pf6N2E2_5118 0.13 +0.4 5,900,130 + Pf6N2E2_5118 0.13 +0.5 5,900,130 + Pf6N2E2_5118 0.13 -0.4 5,900,131 - Pf6N2E2_5118 0.13 +0.6 5,900,137 - Pf6N2E2_5118 0.13 +1.4 5,900,140 + Pf6N2E2_5118 0.14 -0.2 5,900,140 + Pf6N2E2_5118 0.14 -0.0 5,900,140 + Pf6N2E2_5118 0.14 +0.8 5,900,141 - Pf6N2E2_5118 0.14 +0.1 5,900,141 - Pf6N2E2_5118 0.14 +0.4 5,900,141 - Pf6N2E2_5118 0.14 +0.1 5,900,149 + Pf6N2E2_5118 0.14 +0.2 5,900,150 - Pf6N2E2_5118 0.14 +1.3
Or see this region's nucleotide sequence