Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5078 and Pf6N2E2_5079 are separated by 15 nucleotides Pf6N2E2_5079 and Pf6N2E2_5080 are separated by 488 nucleotides Pf6N2E2_5080 and Pf6N2E2_5081 are separated by 140 nucleotides Pf6N2E2_5081 and Pf6N2E2_5082 are separated by 316 nucleotides
Pf6N2E2_5078 - Carbon starvation protein A, at 5,854,311 to 5,856,377
_5078
Pf6N2E2_5079 - COG2879, Hypothetical small protein yjiX, at 5,856,393 to 5,856,590
_5079
Pf6N2E2_5080 - Putative metal chaperone, involved in Zn homeostasis, GTPase of COG0523 family, at 5,857,079 to 5,858,047
_5080
Pf6N2E2_5081 - hypothetical protein, at 5,858,188 to 5,858,631
_5081
Pf6N2E2_5082 - Serine hydroxymethyltransferase (EC 2.1.2.1), at 5,858,948 to 5,860,201
_5082
Position (kb) 5857
5858
5859 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5856.291 kb on + strand at 5856.291 kb on + strand at 5856.291 kb on + strand at 5856.379 kb on - strand at 5856.991 kb on + strand at 5856.991 kb on + strand at 5857.313 kb on + strand, within Pf6N2E2_5080 at 5857.313 kb on + strand, within Pf6N2E2_5080 at 5857.313 kb on + strand, within Pf6N2E2_5080 at 5857.313 kb on + strand, within Pf6N2E2_5080 at 5857.313 kb on + strand, within Pf6N2E2_5080 at 5857.313 kb on + strand, within Pf6N2E2_5080 at 5857.313 kb on + strand, within Pf6N2E2_5080 at 5857.314 kb on - strand, within Pf6N2E2_5080 at 5857.314 kb on - strand, within Pf6N2E2_5080 at 5857.314 kb on - strand, within Pf6N2E2_5080 at 5857.314 kb on - strand, within Pf6N2E2_5080 at 5857.442 kb on + strand, within Pf6N2E2_5080 at 5857.442 kb on + strand, within Pf6N2E2_5080 at 5857.443 kb on - strand, within Pf6N2E2_5080 at 5857.443 kb on - strand, within Pf6N2E2_5080 at 5857.443 kb on - strand, within Pf6N2E2_5080 at 5857.627 kb on + strand, within Pf6N2E2_5080 at 5857.627 kb on + strand, within Pf6N2E2_5080 at 5857.627 kb on + strand, within Pf6N2E2_5080 at 5857.628 kb on - strand, within Pf6N2E2_5080 at 5857.628 kb on - strand, within Pf6N2E2_5080 at 5857.628 kb on - strand, within Pf6N2E2_5080 at 5857.859 kb on + strand, within Pf6N2E2_5080 at 5857.860 kb on - strand, within Pf6N2E2_5080 at 5857.902 kb on + strand, within Pf6N2E2_5080 at 5857.903 kb on - strand, within Pf6N2E2_5080 at 5857.903 kb on - strand, within Pf6N2E2_5080 at 5857.915 kb on - strand, within Pf6N2E2_5080 at 5857.983 kb on + strand at 5857.983 kb on + strand at 5857.983 kb on + strand at 5857.984 kb on - strand at 5857.984 kb on - strand at 5857.984 kb on - strand at 5857.984 kb on - strand at 5858.153 kb on - strand at 5858.200 kb on + strand at 5858.201 kb on - strand at 5858.243 kb on + strand, within Pf6N2E2_5081 at 5858.243 kb on + strand, within Pf6N2E2_5081 at 5858.269 kb on + strand, within Pf6N2E2_5081 at 5858.404 kb on + strand, within Pf6N2E2_5081 at 5858.450 kb on + strand, within Pf6N2E2_5081 at 5858.450 kb on + strand, within Pf6N2E2_5081 at 5858.640 kb on + strand at 5858.723 kb on + strand at 5858.752 kb on - strand at 5858.775 kb on + strand at 5858.803 kb on + strand at 5858.809 kb on + strand at 5858.893 kb on + strand at 5858.930 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 5,856,291 + +1.0 5,856,291 + -0.2 5,856,291 + -0.0 5,856,379 - -0.5 5,856,991 + -0.6 5,856,991 + +0.4 5,857,313 + Pf6N2E2_5080 0.24 -0.9 5,857,313 + Pf6N2E2_5080 0.24 -2.7 5,857,313 + Pf6N2E2_5080 0.24 -2.1 5,857,313 + Pf6N2E2_5080 0.24 -0.4 5,857,313 + Pf6N2E2_5080 0.24 -2.5 5,857,313 + Pf6N2E2_5080 0.24 -0.6 5,857,313 + Pf6N2E2_5080 0.24 -1.9 5,857,314 - Pf6N2E2_5080 0.24 -1.4 5,857,314 - Pf6N2E2_5080 0.24 -1.4 5,857,314 - Pf6N2E2_5080 0.24 +0.4 5,857,314 - Pf6N2E2_5080 0.24 -1.9 5,857,442 + Pf6N2E2_5080 0.37 -2.1 5,857,442 + Pf6N2E2_5080 0.37 -3.3 5,857,443 - Pf6N2E2_5080 0.38 -2.0 5,857,443 - Pf6N2E2_5080 0.38 -3.0 5,857,443 - Pf6N2E2_5080 0.38 -2.2 5,857,627 + Pf6N2E2_5080 0.57 -2.3 5,857,627 + Pf6N2E2_5080 0.57 +0.6 5,857,627 + Pf6N2E2_5080 0.57 -2.0 5,857,628 - Pf6N2E2_5080 0.57 -3.5 5,857,628 - Pf6N2E2_5080 0.57 -1.9 5,857,628 - Pf6N2E2_5080 0.57 -1.6 5,857,859 + Pf6N2E2_5080 0.80 -2.4 5,857,860 - Pf6N2E2_5080 0.81 -1.8 5,857,902 + Pf6N2E2_5080 0.85 -1.0 5,857,903 - Pf6N2E2_5080 0.85 -3.2 5,857,903 - Pf6N2E2_5080 0.85 -1.1 5,857,915 - Pf6N2E2_5080 0.86 -1.9 5,857,983 + +0.4 5,857,983 + -1.8 5,857,983 + -1.7 5,857,984 - -0.6 5,857,984 - -2.9 5,857,984 - -2.2 5,857,984 - +0.4 5,858,153 - -1.8 5,858,200 + +0.8 5,858,201 - +0.3 5,858,243 + Pf6N2E2_5081 0.12 +0.1 5,858,243 + Pf6N2E2_5081 0.12 -0.0 5,858,269 + Pf6N2E2_5081 0.18 -0.4 5,858,404 + Pf6N2E2_5081 0.49 +0.0 5,858,450 + Pf6N2E2_5081 0.59 -0.2 5,858,450 + Pf6N2E2_5081 0.59 +0.4 5,858,640 + +0.4 5,858,723 + +0.4 5,858,752 - +0.4 5,858,775 + -0.7 5,858,803 + +0.1 5,858,809 + +0.0 5,858,893 + +0.0 5,858,930 + -0.8
Or see this region's nucleotide sequence