Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5020 and Pf6N2E2_5021 are separated by 185 nucleotides Pf6N2E2_5021 and Pf6N2E2_5022 are separated by 217 nucleotides Pf6N2E2_5022 and Pf6N2E2_5023 are separated by 28 nucleotides Pf6N2E2_5023 and Pf6N2E2_5024 are separated by 17 nucleotides
Pf6N2E2_5020 - FIG00955339: hypothetical protein, at 5,786,151 to 5,786,480
_5020
Pf6N2E2_5021 - FOG: TPR repeat, SEL1 subfamily, at 5,786,666 to 5,787,220
_5021
Pf6N2E2_5022 - Glutamate-1-semialdehyde aminotransferase (EC 5.4.3.8), at 5,787,438 to 5,788,721
_5022
Pf6N2E2_5023 - Thiamin-phosphate pyrophosphorylase (EC 2.5.1.3), at 5,788,750 to 5,789,376
_5023
Pf6N2E2_5024 - Hydroxymethylpyrimidine phosphate kinase ThiD (EC 2.7.4.7), at 5,789,394 to 5,790,122
_5024
Position (kb)
5787
5788
5789 Strain fitness (log2 ratio)
-1
0
1
2 at 5786.478 kb on + strand at 5786.478 kb on + strand at 5786.478 kb on + strand at 5786.478 kb on + strand at 5786.478 kb on + strand at 5786.478 kb on + strand at 5786.478 kb on + strand at 5786.479 kb on - strand at 5786.479 kb on - strand at 5786.479 kb on - strand at 5786.479 kb on - strand at 5786.479 kb on - strand at 5786.492 kb on + strand at 5786.492 kb on + strand at 5786.527 kb on - strand at 5786.527 kb on - strand at 5786.626 kb on + strand at 5786.627 kb on - strand at 5786.689 kb on + strand at 5786.689 kb on + strand at 5787.289 kb on - strand at 5787.289 kb on - strand at 5787.289 kb on - strand at 5787.289 kb on - strand at 5787.305 kb on - strand at 5787.305 kb on - strand at 5787.305 kb on - strand at 5787.305 kb on - strand at 5787.305 kb on - strand at 5787.343 kb on + strand at 5787.344 kb on - strand at 5787.344 kb on - strand at 5787.344 kb on - strand at 5787.344 kb on - strand at 5787.347 kb on - strand at 5787.347 kb on - strand at 5787.347 kb on - strand at 5787.347 kb on - strand at 5787.366 kb on - strand at 5787.366 kb on - strand at 5787.366 kb on - strand at 5787.366 kb on - strand at 5787.366 kb on - strand at 5787.366 kb on - strand at 5787.370 kb on - strand at 5787.439 kb on + strand at 5787.439 kb on + strand at 5787.440 kb on - strand at 5788.752 kb on - strand at 5788.752 kb on - strand at 5789.010 kb on - strand, within Pf6N2E2_5023 at 5789.010 kb on - strand, within Pf6N2E2_5023 at 5789.010 kb on - strand, within Pf6N2E2_5023 at 5789.309 kb on - strand, within Pf6N2E2_5023 at 5789.309 kb on - strand, within Pf6N2E2_5023 at 5789.309 kb on - strand, within Pf6N2E2_5023 at 5789.309 kb on - strand, within Pf6N2E2_5023 at 5789.313 kb on - strand, within Pf6N2E2_5023 at 5789.313 kb on - strand, within Pf6N2E2_5023 at 5789.313 kb on - strand, within Pf6N2E2_5023 at 5789.313 kb on - strand, within Pf6N2E2_5023 at 5789.358 kb on - strand at 5789.369 kb on - strand at 5789.480 kb on - strand, within Pf6N2E2_5024 at 5789.480 kb on - strand, within Pf6N2E2_5024 at 5789.573 kb on - strand, within Pf6N2E2_5024 at 5789.598 kb on - strand, within Pf6N2E2_5024
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 5,786,478 + -0.5 5,786,478 + +1.4 5,786,478 + +0.6 5,786,478 + -0.8 5,786,478 + +0.5 5,786,478 + -0.2 5,786,478 + -0.5 5,786,479 - +0.2 5,786,479 - -0.1 5,786,479 - -0.1 5,786,479 - +0.6 5,786,479 - -1.4 5,786,492 + -0.7 5,786,492 + -0.3 5,786,527 - +0.3 5,786,527 - +0.4 5,786,626 + +0.4 5,786,627 - +1.1 5,786,689 + -0.9 5,786,689 + -0.4 5,787,289 - +0.8 5,787,289 - +0.4 5,787,289 - +0.1 5,787,289 - +0.4 5,787,305 - +0.4 5,787,305 - +0.3 5,787,305 - -0.2 5,787,305 - -0.3 5,787,305 - +1.4 5,787,343 + +1.4 5,787,344 - -0.6 5,787,344 - +0.4 5,787,344 - -1.1 5,787,344 - +1.4 5,787,347 - -0.3 5,787,347 - -0.6 5,787,347 - +2.0 5,787,347 - +1.4 5,787,366 - +0.0 5,787,366 - +0.8 5,787,366 - -0.0 5,787,366 - +0.3 5,787,366 - -0.5 5,787,366 - -0.8 5,787,370 - +1.4 5,787,439 + -0.9 5,787,439 + -1.0 5,787,440 - +1.2 5,788,752 - -0.7 5,788,752 - -0.3 5,789,010 - Pf6N2E2_5023 0.41 +0.3 5,789,010 - Pf6N2E2_5023 0.41 +0.2 5,789,010 - Pf6N2E2_5023 0.41 +0.6 5,789,309 - Pf6N2E2_5023 0.89 +0.1 5,789,309 - Pf6N2E2_5023 0.89 +0.8 5,789,309 - Pf6N2E2_5023 0.89 -0.5 5,789,309 - Pf6N2E2_5023 0.89 -0.2 5,789,313 - Pf6N2E2_5023 0.90 +2.1 5,789,313 - Pf6N2E2_5023 0.90 -0.4 5,789,313 - Pf6N2E2_5023 0.90 -0.6 5,789,313 - Pf6N2E2_5023 0.90 -0.5 5,789,358 - -1.0 5,789,369 - +0.0 5,789,480 - Pf6N2E2_5024 0.12 -0.2 5,789,480 - Pf6N2E2_5024 0.12 +0.0 5,789,573 - Pf6N2E2_5024 0.25 -0.1 5,789,598 - Pf6N2E2_5024 0.28 -0.3
Or see this region's nucleotide sequence