Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4750

Experiment: Nitrate reduction, replicate A

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction, replicate A
5,485,025	+				+0.7
5,485,047	+				+0.7
5,485,047	+				-1.6
5,485,047	+				+0.7
5,485,047	+				+0.3
5,485,048	-				-0.7
5,485,048	-				-1.8
5,485,048	-				-0.9
5,485,049	+				+0.4
5,485,189	+				+2.4
5,485,240	+				-0.7
5,485,240	+				-0.1
5,485,240	+				-0.5
5,485,544	+		Pf6N2E2_4749	0.42	-1.0
5,485,544	+		Pf6N2E2_4749	0.42	-0.6
5,485,544	+		Pf6N2E2_4749	0.42	-0.7
5,485,544	+		Pf6N2E2_4749	0.42	-1.6
5,485,545	-		Pf6N2E2_4749	0.42	-0.4
5,485,545	-		Pf6N2E2_4749	0.42	-0.8
5,485,978	+				-0.0
5,486,084	-				+0.3
5,486,146	+				-0.1
5,486,146	+				-0.1
5,486,146	+				-0.5
5,486,147	-				+0.0
5,486,158	+				+0.4
5,486,159	-				+1.2
5,486,159	-				+1.2
5,486,222	+		Pf6N2E2_4750	0.13	+0.5
5,486,222	+		Pf6N2E2_4750	0.13	-0.2
5,486,222	+		Pf6N2E2_4750	0.13	-0.0
5,486,223	-		Pf6N2E2_4750	0.13	-0.2
5,486,223	-		Pf6N2E2_4750	0.13	+0.6
5,486,223	-		Pf6N2E2_4750	0.13	+0.3
5,486,223	-		Pf6N2E2_4750	0.13	+0.4
5,486,494	+		Pf6N2E2_4750	0.27	+0.5
5,486,494	+		Pf6N2E2_4750	0.27	+1.4
5,486,494	+		Pf6N2E2_4750	0.27	-0.0
5,486,494	+		Pf6N2E2_4750	0.27	+0.6
5,486,494	+		Pf6N2E2_4750	0.27	+0.1
5,486,494	+		Pf6N2E2_4750	0.27	+0.0
5,486,494	+		Pf6N2E2_4750	0.27	-0.3
5,486,495	-		Pf6N2E2_4750	0.27	-1.6
5,486,495	-		Pf6N2E2_4750	0.27	+0.3
5,486,495	-		Pf6N2E2_4750	0.27	-0.4
5,486,495	-		Pf6N2E2_4750	0.27	-0.4
5,486,670	+		Pf6N2E2_4750	0.36	+0.8
5,486,832	+		Pf6N2E2_4750	0.45	-0.1
5,486,832	+		Pf6N2E2_4750	0.45	-0.1
5,486,832	+		Pf6N2E2_4750	0.45	+0.7
5,486,832	+		Pf6N2E2_4750	0.45	-0.4
5,486,833	-		Pf6N2E2_4750	0.45	+0.4
5,486,844	+		Pf6N2E2_4750	0.45	+0.9
5,486,844	+		Pf6N2E2_4750	0.45	+0.3
5,486,844	+		Pf6N2E2_4750	0.45	+0.5
5,486,845	-				-1.1
5,486,845	-		Pf6N2E2_4750	0.45	+0.1
5,486,845	-		Pf6N2E2_4750	0.45	+0.4
5,486,845	-		Pf6N2E2_4750	0.45	+0.6
5,486,950	-		Pf6N2E2_4750	0.51	-0.2
5,487,168	+		Pf6N2E2_4750	0.62	+0.3
5,487,169	-		Pf6N2E2_4750	0.62	+0.4
5,487,169	-		Pf6N2E2_4750	0.62	+0.5
5,487,169	-		Pf6N2E2_4750	0.62	+0.7
5,487,169	-		Pf6N2E2_4750	0.62	-0.6
5,487,386	-		Pf6N2E2_4750	0.73	+1.4
5,487,653	+		Pf6N2E2_4750	0.87	-0.8
5,487,654	-		Pf6N2E2_4750	0.87	+0.8
5,487,670	-		Pf6N2E2_4750	0.88	+0.3
5,487,694	+		Pf6N2E2_4750	0.89	+0.1
5,487,694	+		Pf6N2E2_4750	0.89	+1.0
5,487,694	+		Pf6N2E2_4750	0.89	+1.8
5,487,694	+		Pf6N2E2_4750	0.89	+1.2
5,487,694	+		Pf6N2E2_4750	0.89	+1.3
5,487,694	+		Pf6N2E2_4750	0.89	+0.2
5,487,695	-		Pf6N2E2_4750	0.89	+0.1
5,487,695	-		Pf6N2E2_4750	0.89	-0.6
5,487,695	-		Pf6N2E2_4750	0.89	+1.0
5,487,695	-		Pf6N2E2_4750	0.89	-0.2
5,487,749	-				-0.9
5,487,779	-				+0.3
5,487,903	-				-0.2
5,487,909	+				+1.0
5,487,909	+				+2.4
5,487,910	-				-2.2
5,487,987	+				+2.1
5,487,987	+				+0.2
5,487,987	+				+1.5
5,487,987	+				+0.9
5,487,987	+				+0.1
5,487,987	+				-0.2
5,487,987	+				-0.6
5,487,987	+				+0.9
5,487,987	+				-0.3
5,487,987	+				+0.9
5,487,987	+				+2.0
5,487,987	+				+1.2
5,487,987	+				+1.9
5,487,987	+				+1.0
5,487,988	-				-2.9
5,487,988	-				+0.1
5,487,988	-				-2.7
5,487,988	-				+0.3
5,487,988	-				-2.5
5,487,988	-				-1.4
5,487,988	-				-1.8
5,487,988	-				-1.1
5,487,988	-				+1.0
5,487,993	+				+1.4
5,487,994	-				-2.6
5,488,006	-				-1.2
5,488,017	+				+1.4
5,488,018	-				-1.7
5,488,018	-				-3.1
5,488,030	+				-0.5
5,488,057	+				+1.0
5,488,057	+				+1.2
5,488,057	+				+1.4
5,488,057	+				+1.5
5,488,058	-				-0.2
5,488,058	-				-0.9
5,488,058	-				-0.7
5,488,058	-				-0.9
5,488,058	-				-1.0
5,488,141	+				+1.4
5,488,142	-				-0.9
5,488,150	+				+1.4
5,488,150	+				+0.4
5,488,150	+				+1.2
5,488,150	+				+1.6
5,488,150	+				+1.6
5,488,150	+				+0.9
5,488,150	+				+0.5
5,488,150	+				+1.3
5,488,151	-				-0.9
5,488,151	-				-0.4
5,488,175	+				-0.1
5,488,175	+				-0.4
5,488,175	+				-1.2
5,488,176	-				-2.1
5,488,427	+		Pf6N2E2_4751	0.60	-0.4
5,488,427	+		Pf6N2E2_4751	0.60	+1.4
5,488,428	-		Pf6N2E2_4751	0.60	-1.9
5,488,428	-		Pf6N2E2_4751	0.60	-0.4
5,488,477	+		Pf6N2E2_4751	0.71	+0.4
5,488,605	+				-0.6
5,488,605	+				+0.3
5,488,695	+				+0.1
5,488,695	+				+0.4
5,488,696	-				-0.5
5,488,772	+				-0.6
5,488,772	+				-0.2
5,488,772	+				-0.1
5,488,772	+				+0.8
5,488,772	+				-0.8
5,488,772	+				-0.3
5,488,773	-				-0.8
5,488,773	-				-0.2
5,488,894	+				+1.1
5,488,895	-				+0.9