Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4636 and Pf6N2E2_4637 are separated by 106 nucleotides Pf6N2E2_4637 and Pf6N2E2_4638 are separated by 11 nucleotides Pf6N2E2_4638 and Pf6N2E2_4639 are separated by 167 nucleotides
Pf6N2E2_4636 - periplasmic binding protein, putative, at 5,366,863 to 5,367,588
_4636
Pf6N2E2_4637 - hypothetical protein, at 5,367,695 to 5,367,838
_4637
Pf6N2E2_4638 - 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20), at 5,367,850 to 5,368,695
_4638
Pf6N2E2_4639 - Adenosylhomocysteinase (EC 3.3.1.1), at 5,368,863 to 5,370,272
_4639
Position (kb)
5367
5368
5369 Strain fitness (log2 ratio)
-2
-1
0
1 at 5366.859 kb on + strand at 5366.859 kb on + strand at 5366.860 kb on - strand at 5366.936 kb on + strand, within Pf6N2E2_4636 at 5367.023 kb on + strand, within Pf6N2E2_4636 at 5367.023 kb on + strand, within Pf6N2E2_4636 at 5367.222 kb on - strand, within Pf6N2E2_4636 at 5367.303 kb on - strand, within Pf6N2E2_4636 at 5367.319 kb on + strand, within Pf6N2E2_4636 at 5367.319 kb on + strand, within Pf6N2E2_4636 at 5367.319 kb on + strand, within Pf6N2E2_4636 at 5367.320 kb on - strand, within Pf6N2E2_4636 at 5367.320 kb on - strand, within Pf6N2E2_4636 at 5367.320 kb on - strand, within Pf6N2E2_4636 at 5367.320 kb on - strand, within Pf6N2E2_4636 at 5367.497 kb on + strand, within Pf6N2E2_4636 at 5367.497 kb on + strand, within Pf6N2E2_4636 at 5367.498 kb on - strand, within Pf6N2E2_4636 at 5367.498 kb on - strand, within Pf6N2E2_4636 at 5367.530 kb on + strand at 5367.531 kb on - strand at 5367.531 kb on - strand at 5367.536 kb on + strand at 5367.537 kb on - strand at 5367.537 kb on - strand at 5367.537 kb on - strand at 5367.537 kb on - strand at 5367.670 kb on + strand at 5367.670 kb on + strand at 5367.670 kb on + strand at 5367.671 kb on - strand at 5367.671 kb on - strand at 5367.671 kb on - strand at 5367.671 kb on - strand at 5367.674 kb on + strand at 5367.674 kb on + strand at 5367.674 kb on + strand at 5367.674 kb on + strand at 5367.675 kb on - strand at 5367.675 kb on - strand at 5367.675 kb on - strand at 5367.675 kb on - strand at 5367.696 kb on + strand at 5367.696 kb on + strand at 5367.697 kb on - strand at 5367.707 kb on + strand at 5367.708 kb on - strand at 5367.708 kb on - strand at 5367.833 kb on + strand at 5367.833 kb on + strand at 5367.834 kb on - strand at 5367.842 kb on - strand at 5367.910 kb on - strand at 5367.911 kb on + strand at 5367.912 kb on - strand at 5367.912 kb on - strand at 5368.010 kb on + strand, within Pf6N2E2_4638 at 5368.085 kb on + strand, within Pf6N2E2_4638 at 5368.086 kb on - strand, within Pf6N2E2_4638 at 5368.086 kb on - strand, within Pf6N2E2_4638 at 5368.163 kb on + strand, within Pf6N2E2_4638 at 5368.163 kb on + strand, within Pf6N2E2_4638 at 5368.163 kb on + strand, within Pf6N2E2_4638 at 5368.164 kb on - strand, within Pf6N2E2_4638 at 5368.164 kb on - strand, within Pf6N2E2_4638 at 5368.571 kb on + strand, within Pf6N2E2_4638 at 5368.571 kb on + strand, within Pf6N2E2_4638 at 5368.571 kb on + strand, within Pf6N2E2_4638 at 5368.599 kb on - strand, within Pf6N2E2_4638 at 5368.599 kb on - strand, within Pf6N2E2_4638 at 5368.677 kb on - strand at 5368.701 kb on + strand at 5368.702 kb on - strand at 5368.718 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 5,366,859 + -0.2 5,366,859 + -0.2 5,366,860 - -0.5 5,366,936 + Pf6N2E2_4636 0.10 -1.8 5,367,023 + Pf6N2E2_4636 0.22 -0.4 5,367,023 + Pf6N2E2_4636 0.22 -1.0 5,367,222 - Pf6N2E2_4636 0.49 -0.4 5,367,303 - Pf6N2E2_4636 0.61 -0.4 5,367,319 + Pf6N2E2_4636 0.63 +1.7 5,367,319 + Pf6N2E2_4636 0.63 -1.4 5,367,319 + Pf6N2E2_4636 0.63 +0.4 5,367,320 - Pf6N2E2_4636 0.63 -0.8 5,367,320 - Pf6N2E2_4636 0.63 -0.1 5,367,320 - Pf6N2E2_4636 0.63 -0.3 5,367,320 - Pf6N2E2_4636 0.63 -2.2 5,367,497 + Pf6N2E2_4636 0.87 +0.5 5,367,497 + Pf6N2E2_4636 0.87 -0.4 5,367,498 - Pf6N2E2_4636 0.87 +0.3 5,367,498 - Pf6N2E2_4636 0.87 -1.5 5,367,530 + -0.2 5,367,531 - +0.1 5,367,531 - -0.6 5,367,536 + -0.5 5,367,537 - -1.3 5,367,537 - +0.8 5,367,537 - +1.3 5,367,537 - -1.4 5,367,670 + -1.0 5,367,670 + -0.6 5,367,670 + -1.1 5,367,671 - +0.0 5,367,671 - +0.4 5,367,671 - -0.0 5,367,671 - -0.7 5,367,674 + -0.3 5,367,674 + +0.3 5,367,674 + +0.1 5,367,674 + -1.0 5,367,675 - -0.2 5,367,675 - +1.3 5,367,675 - +0.7 5,367,675 - -1.3 5,367,696 + +0.5 5,367,696 + -0.8 5,367,697 - +0.3 5,367,707 + -0.9 5,367,708 - -0.6 5,367,708 - +0.2 5,367,833 + +0.4 5,367,833 + -0.8 5,367,834 - +0.3 5,367,842 - +0.2 5,367,910 - -2.4 5,367,911 + +1.3 5,367,912 - +1.3 5,367,912 - -0.8 5,368,010 + Pf6N2E2_4638 0.19 +0.3 5,368,085 + Pf6N2E2_4638 0.28 -2.3 5,368,086 - Pf6N2E2_4638 0.28 +1.3 5,368,086 - Pf6N2E2_4638 0.28 -2.0 5,368,163 + Pf6N2E2_4638 0.37 +1.3 5,368,163 + Pf6N2E2_4638 0.37 -1.5 5,368,163 + Pf6N2E2_4638 0.37 -1.5 5,368,164 - Pf6N2E2_4638 0.37 -0.7 5,368,164 - Pf6N2E2_4638 0.37 -0.3 5,368,571 + Pf6N2E2_4638 0.85 +0.3 5,368,571 + Pf6N2E2_4638 0.85 +1.3 5,368,571 + Pf6N2E2_4638 0.85 -1.0 5,368,599 - Pf6N2E2_4638 0.89 +1.3 5,368,599 - Pf6N2E2_4638 0.89 -1.8 5,368,677 - -2.3 5,368,701 + +1.3 5,368,702 - +0.4 5,368,718 - +0.3
Or see this region's nucleotide sequence