Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4431 and Pf6N2E2_4432 overlap by 4 nucleotides Pf6N2E2_4432 and Pf6N2E2_4433 overlap by 4 nucleotides
Pf6N2E2_4431 - Porphobilinogen deaminase (EC 2.5.1.61), at 5,162,355 to 5,163,296
_4431
Pf6N2E2_4432 - Uroporphyrinogen-III synthase (EC 4.2.1.75), at 5,163,293 to 5,165,209
_4432
Pf6N2E2_4433 - Uncharacterized protein EC-HemY, likely associated with heme metabolism based on gene clustering with hemC, hemD in Proteobacteria (unrelated to HemY-type PPO in GramPositives), at 5,165,206 to 5,166,453
_4433
Position (kb)
5163
5164
5165
5166 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5162.319 kb on + strand at 5162.319 kb on + strand at 5162.331 kb on + strand at 5162.338 kb on + strand at 5162.342 kb on + strand at 5163.273 kb on + strand at 5164.057 kb on + strand, within Pf6N2E2_4432 at 5164.058 kb on - strand, within Pf6N2E2_4432 at 5164.073 kb on + strand, within Pf6N2E2_4432 at 5164.073 kb on + strand, within Pf6N2E2_4432 at 5164.073 kb on + strand, within Pf6N2E2_4432 at 5164.073 kb on + strand, within Pf6N2E2_4432 at 5164.073 kb on + strand, within Pf6N2E2_4432 at 5164.073 kb on + strand, within Pf6N2E2_4432 at 5164.074 kb on - strand, within Pf6N2E2_4432 at 5164.074 kb on - strand, within Pf6N2E2_4432 at 5164.107 kb on + strand, within Pf6N2E2_4432 at 5164.107 kb on + strand, within Pf6N2E2_4432 at 5164.107 kb on + strand, within Pf6N2E2_4432 at 5164.107 kb on + strand, within Pf6N2E2_4432 at 5164.108 kb on - strand, within Pf6N2E2_4432 at 5164.108 kb on - strand, within Pf6N2E2_4432 at 5164.108 kb on - strand, within Pf6N2E2_4432 at 5164.182 kb on + strand, within Pf6N2E2_4432 at 5164.182 kb on + strand, within Pf6N2E2_4432 at 5164.182 kb on + strand, within Pf6N2E2_4432 at 5164.182 kb on + strand, within Pf6N2E2_4432 at 5164.183 kb on - strand, within Pf6N2E2_4432 at 5164.183 kb on - strand, within Pf6N2E2_4432 at 5164.183 kb on - strand, within Pf6N2E2_4432 at 5164.183 kb on - strand, within Pf6N2E2_4432 at 5164.183 kb on - strand, within Pf6N2E2_4432 at 5164.183 kb on - strand, within Pf6N2E2_4432 at 5164.183 kb on - strand, within Pf6N2E2_4432 at 5164.262 kb on - strand, within Pf6N2E2_4432 at 5164.739 kb on + strand, within Pf6N2E2_4432 at 5164.739 kb on + strand, within Pf6N2E2_4432 at 5164.740 kb on - strand, within Pf6N2E2_4432 at 5164.740 kb on - strand, within Pf6N2E2_4432 at 5164.830 kb on - strand, within Pf6N2E2_4432 at 5164.831 kb on + strand, within Pf6N2E2_4432 at 5164.968 kb on - strand, within Pf6N2E2_4432 at 5165.129 kb on + strand at 5165.130 kb on - strand at 5165.130 kb on - strand at 5165.218 kb on + strand at 5165.218 kb on + strand at 5165.219 kb on - strand at 5165.287 kb on + strand at 5165.288 kb on - strand at 5165.302 kb on + strand at 5165.406 kb on + strand, within Pf6N2E2_4433 at 5165.406 kb on + strand, within Pf6N2E2_4433 at 5165.406 kb on + strand, within Pf6N2E2_4433 at 5165.407 kb on - strand, within Pf6N2E2_4433 at 5165.407 kb on - strand, within Pf6N2E2_4433 at 5165.566 kb on + strand, within Pf6N2E2_4433 at 5165.566 kb on + strand, within Pf6N2E2_4433 at 5165.566 kb on + strand, within Pf6N2E2_4433 at 5165.566 kb on + strand, within Pf6N2E2_4433 at 5165.567 kb on - strand, within Pf6N2E2_4433 at 5165.567 kb on - strand, within Pf6N2E2_4433 at 5165.567 kb on - strand, within Pf6N2E2_4433 at 5165.567 kb on - strand, within Pf6N2E2_4433 at 5165.569 kb on + strand, within Pf6N2E2_4433 at 5165.570 kb on - strand, within Pf6N2E2_4433 at 5165.608 kb on + strand, within Pf6N2E2_4433 at 5165.608 kb on + strand, within Pf6N2E2_4433 at 5165.609 kb on - strand, within Pf6N2E2_4433 at 5165.609 kb on - strand, within Pf6N2E2_4433 at 5166.028 kb on + strand, within Pf6N2E2_4433 at 5166.028 kb on + strand, within Pf6N2E2_4433 at 5166.028 kb on + strand, within Pf6N2E2_4433 at 5166.029 kb on - strand, within Pf6N2E2_4433 at 5166.077 kb on + strand, within Pf6N2E2_4433 at 5166.077 kb on + strand, within Pf6N2E2_4433 at 5166.078 kb on - strand, within Pf6N2E2_4433 at 5166.103 kb on + strand, within Pf6N2E2_4433 at 5166.103 kb on + strand, within Pf6N2E2_4433 at 5166.104 kb on - strand, within Pf6N2E2_4433 at 5166.127 kb on + strand, within Pf6N2E2_4433
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 5,162,319 + -0.6 5,162,319 + +0.1 5,162,331 + -0.1 5,162,338 + -0.5 5,162,342 + +1.3 5,163,273 + +0.3 5,164,057 + Pf6N2E2_4432 0.40 -1.0 5,164,058 - Pf6N2E2_4432 0.40 +0.7 5,164,073 + Pf6N2E2_4432 0.41 -0.3 5,164,073 + Pf6N2E2_4432 0.41 -0.5 5,164,073 + Pf6N2E2_4432 0.41 -0.2 5,164,073 + Pf6N2E2_4432 0.41 +0.3 5,164,073 + Pf6N2E2_4432 0.41 -1.1 5,164,073 + Pf6N2E2_4432 0.41 -1.6 5,164,074 - Pf6N2E2_4432 0.41 +2.1 5,164,074 - Pf6N2E2_4432 0.41 +0.1 5,164,107 + Pf6N2E2_4432 0.42 -0.6 5,164,107 + Pf6N2E2_4432 0.42 +0.2 5,164,107 + Pf6N2E2_4432 0.42 +1.3 5,164,107 + Pf6N2E2_4432 0.42 -0.1 5,164,108 - Pf6N2E2_4432 0.43 -0.3 5,164,108 - Pf6N2E2_4432 0.43 +0.6 5,164,108 - Pf6N2E2_4432 0.43 -0.2 5,164,182 + Pf6N2E2_4432 0.46 -1.5 5,164,182 + Pf6N2E2_4432 0.46 -0.7 5,164,182 + Pf6N2E2_4432 0.46 -0.5 5,164,182 + Pf6N2E2_4432 0.46 +0.5 5,164,183 - Pf6N2E2_4432 0.46 -2.4 5,164,183 - Pf6N2E2_4432 0.46 -1.0 5,164,183 - Pf6N2E2_4432 0.46 +1.3 5,164,183 - Pf6N2E2_4432 0.46 -0.1 5,164,183 - Pf6N2E2_4432 0.46 -0.3 5,164,183 - Pf6N2E2_4432 0.46 -2.6 5,164,183 - Pf6N2E2_4432 0.46 -3.0 5,164,262 - Pf6N2E2_4432 0.51 -0.3 5,164,739 + Pf6N2E2_4432 0.75 -0.1 5,164,739 + Pf6N2E2_4432 0.75 +0.2 5,164,740 - Pf6N2E2_4432 0.75 -0.2 5,164,740 - Pf6N2E2_4432 0.75 +0.7 5,164,830 - Pf6N2E2_4432 0.80 -0.5 5,164,831 + Pf6N2E2_4432 0.80 -1.3 5,164,968 - Pf6N2E2_4432 0.87 +0.6 5,165,129 + +0.1 5,165,130 - -0.8 5,165,130 - -0.1 5,165,218 + -1.3 5,165,218 + +1.3 5,165,219 - +0.1 5,165,287 + +0.3 5,165,288 - +0.4 5,165,302 + -0.3 5,165,406 + Pf6N2E2_4433 0.16 -1.4 5,165,406 + Pf6N2E2_4433 0.16 -0.1 5,165,406 + Pf6N2E2_4433 0.16 -0.4 5,165,407 - Pf6N2E2_4433 0.16 +0.5 5,165,407 - Pf6N2E2_4433 0.16 +0.3 5,165,566 + Pf6N2E2_4433 0.29 +0.6 5,165,566 + Pf6N2E2_4433 0.29 -0.7 5,165,566 + Pf6N2E2_4433 0.29 -0.0 5,165,566 + Pf6N2E2_4433 0.29 -1.8 5,165,567 - Pf6N2E2_4433 0.29 +1.1 5,165,567 - Pf6N2E2_4433 0.29 -0.8 5,165,567 - Pf6N2E2_4433 0.29 -0.7 5,165,567 - Pf6N2E2_4433 0.29 +0.6 5,165,569 + Pf6N2E2_4433 0.29 -0.2 5,165,570 - Pf6N2E2_4433 0.29 -0.8 5,165,608 + Pf6N2E2_4433 0.32 -1.0 5,165,608 + Pf6N2E2_4433 0.32 -0.2 5,165,609 - Pf6N2E2_4433 0.32 +0.1 5,165,609 - Pf6N2E2_4433 0.32 -0.7 5,166,028 + Pf6N2E2_4433 0.66 +1.3 5,166,028 + Pf6N2E2_4433 0.66 +0.0 5,166,028 + Pf6N2E2_4433 0.66 +0.2 5,166,029 - Pf6N2E2_4433 0.66 -1.2 5,166,077 + Pf6N2E2_4433 0.70 -0.1 5,166,077 + Pf6N2E2_4433 0.70 -0.3 5,166,078 - Pf6N2E2_4433 0.70 -1.1 5,166,103 + Pf6N2E2_4433 0.72 -0.9 5,166,103 + Pf6N2E2_4433 0.72 -0.0 5,166,104 - Pf6N2E2_4433 0.72 -0.5 5,166,127 + Pf6N2E2_4433 0.74 -0.0
Or see this region's nucleotide sequence