Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4430 and Pf6N2E2_4431 are separated by 137 nucleotides Pf6N2E2_4431 and Pf6N2E2_4432 overlap by 4 nucleotides
Pf6N2E2_4430 - Autolysis response regulater LytR, at 5,161,471 to 5,162,217
_4430
Pf6N2E2_4431 - Porphobilinogen deaminase (EC 2.5.1.61), at 5,162,355 to 5,163,296
_4431
Pf6N2E2_4432 - Uroporphyrinogen-III synthase (EC 4.2.1.75), at 5,163,293 to 5,165,209
_4432
Position (kb)
5162
5163
5164 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5161.390 kb on + strand at 5161.390 kb on + strand at 5161.428 kb on + strand at 5161.428 kb on + strand at 5161.428 kb on + strand at 5161.470 kb on + strand at 5161.471 kb on - strand at 5161.471 kb on - strand at 5161.546 kb on + strand, within Pf6N2E2_4430 at 5161.546 kb on + strand, within Pf6N2E2_4430 at 5161.547 kb on - strand, within Pf6N2E2_4430 at 5161.618 kb on - strand, within Pf6N2E2_4430 at 5161.658 kb on + strand, within Pf6N2E2_4430 at 5161.658 kb on + strand, within Pf6N2E2_4430 at 5161.658 kb on + strand, within Pf6N2E2_4430 at 5161.658 kb on + strand, within Pf6N2E2_4430 at 5161.659 kb on - strand, within Pf6N2E2_4430 at 5161.713 kb on + strand, within Pf6N2E2_4430 at 5161.714 kb on - strand, within Pf6N2E2_4430 at 5161.714 kb on - strand, within Pf6N2E2_4430 at 5161.765 kb on + strand, within Pf6N2E2_4430 at 5161.893 kb on + strand, within Pf6N2E2_4430 at 5161.894 kb on - strand, within Pf6N2E2_4430 at 5161.948 kb on + strand, within Pf6N2E2_4430 at 5161.948 kb on + strand, within Pf6N2E2_4430 at 5161.948 kb on + strand, within Pf6N2E2_4430 at 5161.948 kb on + strand, within Pf6N2E2_4430 at 5161.948 kb on + strand, within Pf6N2E2_4430 at 5161.949 kb on - strand, within Pf6N2E2_4430 at 5161.949 kb on - strand, within Pf6N2E2_4430 at 5161.949 kb on - strand, within Pf6N2E2_4430 at 5161.969 kb on + strand, within Pf6N2E2_4430 at 5161.969 kb on + strand, within Pf6N2E2_4430 at 5161.969 kb on + strand, within Pf6N2E2_4430 at 5161.969 kb on + strand, within Pf6N2E2_4430 at 5161.969 kb on + strand, within Pf6N2E2_4430 at 5161.969 kb on + strand, within Pf6N2E2_4430 at 5161.969 kb on + strand, within Pf6N2E2_4430 at 5161.969 kb on + strand, within Pf6N2E2_4430 at 5161.969 kb on + strand, within Pf6N2E2_4430 at 5161.970 kb on - strand, within Pf6N2E2_4430 at 5161.970 kb on - strand, within Pf6N2E2_4430 at 5161.970 kb on - strand, within Pf6N2E2_4430 at 5161.970 kb on - strand, within Pf6N2E2_4430 at 5161.970 kb on - strand, within Pf6N2E2_4430 at 5161.970 kb on - strand, within Pf6N2E2_4430 at 5161.970 kb on - strand, within Pf6N2E2_4430 at 5162.215 kb on + strand at 5162.215 kb on + strand at 5162.215 kb on + strand at 5162.278 kb on + strand at 5162.319 kb on + strand at 5162.319 kb on + strand at 5162.331 kb on + strand at 5162.338 kb on + strand at 5162.342 kb on + strand at 5163.273 kb on + strand at 5164.057 kb on + strand, within Pf6N2E2_4432 at 5164.058 kb on - strand, within Pf6N2E2_4432 at 5164.073 kb on + strand, within Pf6N2E2_4432 at 5164.073 kb on + strand, within Pf6N2E2_4432 at 5164.073 kb on + strand, within Pf6N2E2_4432 at 5164.073 kb on + strand, within Pf6N2E2_4432 at 5164.073 kb on + strand, within Pf6N2E2_4432 at 5164.073 kb on + strand, within Pf6N2E2_4432 at 5164.074 kb on - strand, within Pf6N2E2_4432 at 5164.074 kb on - strand, within Pf6N2E2_4432 at 5164.107 kb on + strand, within Pf6N2E2_4432 at 5164.107 kb on + strand, within Pf6N2E2_4432 at 5164.107 kb on + strand, within Pf6N2E2_4432 at 5164.107 kb on + strand, within Pf6N2E2_4432 at 5164.108 kb on - strand, within Pf6N2E2_4432 at 5164.108 kb on - strand, within Pf6N2E2_4432 at 5164.108 kb on - strand, within Pf6N2E2_4432 at 5164.182 kb on + strand, within Pf6N2E2_4432 at 5164.182 kb on + strand, within Pf6N2E2_4432 at 5164.182 kb on + strand, within Pf6N2E2_4432 at 5164.182 kb on + strand, within Pf6N2E2_4432 at 5164.183 kb on - strand, within Pf6N2E2_4432 at 5164.183 kb on - strand, within Pf6N2E2_4432 at 5164.183 kb on - strand, within Pf6N2E2_4432 at 5164.183 kb on - strand, within Pf6N2E2_4432 at 5164.183 kb on - strand, within Pf6N2E2_4432 at 5164.183 kb on - strand, within Pf6N2E2_4432 at 5164.183 kb on - strand, within Pf6N2E2_4432 at 5164.262 kb on - strand, within Pf6N2E2_4432
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 5,161,390 + +1.3 5,161,390 + -0.2 5,161,428 + -0.1 5,161,428 + -0.2 5,161,428 + +1.0 5,161,470 + -0.2 5,161,471 - -0.5 5,161,471 - -0.5 5,161,546 + Pf6N2E2_4430 0.10 +0.7 5,161,546 + Pf6N2E2_4430 0.10 -0.9 5,161,547 - Pf6N2E2_4430 0.10 -0.6 5,161,618 - Pf6N2E2_4430 0.20 -1.2 5,161,658 + Pf6N2E2_4430 0.25 -0.1 5,161,658 + Pf6N2E2_4430 0.25 +0.0 5,161,658 + Pf6N2E2_4430 0.25 -1.2 5,161,658 + Pf6N2E2_4430 0.25 +1.0 5,161,659 - Pf6N2E2_4430 0.25 -0.3 5,161,713 + Pf6N2E2_4430 0.32 -0.2 5,161,714 - Pf6N2E2_4430 0.33 +0.5 5,161,714 - Pf6N2E2_4430 0.33 +0.6 5,161,765 + Pf6N2E2_4430 0.39 -0.2 5,161,893 + Pf6N2E2_4430 0.56 +0.2 5,161,894 - Pf6N2E2_4430 0.57 +0.3 5,161,948 + Pf6N2E2_4430 0.64 -0.7 5,161,948 + Pf6N2E2_4430 0.64 +0.7 5,161,948 + Pf6N2E2_4430 0.64 +0.4 5,161,948 + Pf6N2E2_4430 0.64 +0.2 5,161,948 + Pf6N2E2_4430 0.64 -0.4 5,161,949 - Pf6N2E2_4430 0.64 -0.1 5,161,949 - Pf6N2E2_4430 0.64 -0.6 5,161,949 - Pf6N2E2_4430 0.64 +0.3 5,161,969 + Pf6N2E2_4430 0.67 -0.5 5,161,969 + Pf6N2E2_4430 0.67 +0.6 5,161,969 + Pf6N2E2_4430 0.67 -0.2 5,161,969 + Pf6N2E2_4430 0.67 +0.2 5,161,969 + Pf6N2E2_4430 0.67 +0.7 5,161,969 + Pf6N2E2_4430 0.67 -0.9 5,161,969 + Pf6N2E2_4430 0.67 -0.2 5,161,969 + Pf6N2E2_4430 0.67 -0.1 5,161,969 + Pf6N2E2_4430 0.67 -0.4 5,161,970 - Pf6N2E2_4430 0.67 -0.1 5,161,970 - Pf6N2E2_4430 0.67 -0.6 5,161,970 - Pf6N2E2_4430 0.67 -0.5 5,161,970 - Pf6N2E2_4430 0.67 -1.6 5,161,970 - Pf6N2E2_4430 0.67 -0.0 5,161,970 - Pf6N2E2_4430 0.67 -0.3 5,161,970 - Pf6N2E2_4430 0.67 +0.8 5,162,215 + +0.2 5,162,215 + -1.3 5,162,215 + -0.6 5,162,278 + -0.1 5,162,319 + -0.6 5,162,319 + +0.1 5,162,331 + -0.1 5,162,338 + -0.5 5,162,342 + +1.3 5,163,273 + +0.3 5,164,057 + Pf6N2E2_4432 0.40 -1.0 5,164,058 - Pf6N2E2_4432 0.40 +0.7 5,164,073 + Pf6N2E2_4432 0.41 -0.3 5,164,073 + Pf6N2E2_4432 0.41 -0.5 5,164,073 + Pf6N2E2_4432 0.41 -0.2 5,164,073 + Pf6N2E2_4432 0.41 +0.3 5,164,073 + Pf6N2E2_4432 0.41 -1.1 5,164,073 + Pf6N2E2_4432 0.41 -1.6 5,164,074 - Pf6N2E2_4432 0.41 +2.1 5,164,074 - Pf6N2E2_4432 0.41 +0.1 5,164,107 + Pf6N2E2_4432 0.42 -0.6 5,164,107 + Pf6N2E2_4432 0.42 +0.2 5,164,107 + Pf6N2E2_4432 0.42 +1.3 5,164,107 + Pf6N2E2_4432 0.42 -0.1 5,164,108 - Pf6N2E2_4432 0.43 -0.3 5,164,108 - Pf6N2E2_4432 0.43 +0.6 5,164,108 - Pf6N2E2_4432 0.43 -0.2 5,164,182 + Pf6N2E2_4432 0.46 -1.5 5,164,182 + Pf6N2E2_4432 0.46 -0.7 5,164,182 + Pf6N2E2_4432 0.46 -0.5 5,164,182 + Pf6N2E2_4432 0.46 +0.5 5,164,183 - Pf6N2E2_4432 0.46 -2.4 5,164,183 - Pf6N2E2_4432 0.46 -1.0 5,164,183 - Pf6N2E2_4432 0.46 +1.3 5,164,183 - Pf6N2E2_4432 0.46 -0.1 5,164,183 - Pf6N2E2_4432 0.46 -0.3 5,164,183 - Pf6N2E2_4432 0.46 -2.6 5,164,183 - Pf6N2E2_4432 0.46 -3.0 5,164,262 - Pf6N2E2_4432 0.51 -0.3
Or see this region's nucleotide sequence