Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_431

Experiment: Nitrate reduction, replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_430 and Pf6N2E2_431 are separated by 3 nucleotidesPf6N2E2_431 and Pf6N2E2_432 are separated by 1 nucleotidesPf6N2E2_432 and Pf6N2E2_433 are separated by 128 nucleotides Pf6N2E2_430 - Molybdenum transport ATP-binding protein ModC (TC 3.A.1.8.1), at 497,691 to 498,770 _430 Pf6N2E2_431 - Molybdenum transport system permease protein ModB (TC 3.A.1.8.1), at 498,774 to 499,454 _431 Pf6N2E2_432 - Molybdenum ABC transporter, periplasmic molybdenum-binding protein ModA (TC 3.A.1.8.1), at 499,456 to 500,208 _432 Pf6N2E2_433 - Enoyl-[acyl-carrier-protein] reductase [FMN] (EC 1.3.1.9), at 500,337 to 501,410 _433 Position (kb) 498 499 500Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 497.982 kb on + strand, within Pf6N2E2_430at 497.982 kb on + strand, within Pf6N2E2_430at 497.983 kb on - strand, within Pf6N2E2_430at 498.025 kb on + strand, within Pf6N2E2_430at 498.025 kb on + strand, within Pf6N2E2_430at 498.026 kb on - strand, within Pf6N2E2_430at 498.208 kb on + strand, within Pf6N2E2_430at 498.208 kb on + strandat 498.208 kb on + strand, within Pf6N2E2_430at 498.208 kb on + strand, within Pf6N2E2_430at 498.208 kb on + strand, within Pf6N2E2_430at 498.208 kb on + strand, within Pf6N2E2_430at 498.208 kb on + strand, within Pf6N2E2_430at 498.209 kb on - strand, within Pf6N2E2_430at 498.209 kb on - strand, within Pf6N2E2_430at 498.209 kb on - strand, within Pf6N2E2_430at 498.209 kb on - strand, within Pf6N2E2_430at 498.209 kb on - strand, within Pf6N2E2_430at 498.209 kb on - strand, within Pf6N2E2_430at 498.250 kb on + strand, within Pf6N2E2_430at 498.532 kb on + strand, within Pf6N2E2_430at 498.533 kb on - strand, within Pf6N2E2_430at 498.533 kb on - strand, within Pf6N2E2_430at 498.609 kb on - strand, within Pf6N2E2_430at 498.662 kb on + strand, within Pf6N2E2_430at 498.683 kb on - strandat 498.809 kb on - strandat 498.875 kb on - strand, within Pf6N2E2_431at 498.899 kb on - strand, within Pf6N2E2_431at 498.899 kb on - strand, within Pf6N2E2_431at 498.899 kb on - strand, within Pf6N2E2_431at 499.150 kb on + strand, within Pf6N2E2_431at 499.150 kb on + strand, within Pf6N2E2_431at 499.151 kb on - strand, within Pf6N2E2_431at 499.151 kb on - strand, within Pf6N2E2_431at 499.151 kb on - strand, within Pf6N2E2_431at 499.151 kb on - strand, within Pf6N2E2_431at 499.151 kb on - strand, within Pf6N2E2_431at 499.151 kb on - strand, within Pf6N2E2_431at 499.261 kb on + strand, within Pf6N2E2_431at 499.262 kb on - strand, within Pf6N2E2_431at 499.262 kb on - strand, within Pf6N2E2_431at 499.262 kb on - strand, within Pf6N2E2_431at 499.353 kb on + strand, within Pf6N2E2_431at 499.354 kb on - strand, within Pf6N2E2_431at 499.432 kb on + strandat 499.433 kb on - strandat 499.457 kb on + strandat 499.511 kb on + strandat 499.511 kb on + strandat 499.512 kb on - strandat 499.640 kb on + strand, within Pf6N2E2_432at 499.640 kb on + strand, within Pf6N2E2_432at 499.640 kb on + strand, within Pf6N2E2_432at 499.868 kb on + strand, within Pf6N2E2_432at 499.868 kb on + strand, within Pf6N2E2_432at 499.868 kb on + strand, within Pf6N2E2_432at 499.868 kb on + strand, within Pf6N2E2_432at 499.868 kb on + strand, within Pf6N2E2_432at 499.869 kb on - strand, within Pf6N2E2_432at 499.869 kb on - strand, within Pf6N2E2_432at 499.911 kb on - strand, within Pf6N2E2_432at 499.911 kb on - strand, within Pf6N2E2_432at 500.021 kb on + strand, within Pf6N2E2_432at 500.021 kb on + strand, within Pf6N2E2_432at 500.021 kb on + strand, within Pf6N2E2_432at 500.058 kb on + strand, within Pf6N2E2_432at 500.058 kb on + strand, within Pf6N2E2_432at 500.160 kb on + strandat 500.160 kb on + strandat 500.160 kb on + strandat 500.161 kb on - strandat 500.208 kb on + strandat 500.208 kb on + strandat 500.208 kb on + strandat 500.208 kb on + strandat 500.208 kb on + strandat 500.208 kb on + strandat 500.209 kb on - strandat 500.209 kb on - strandat 500.209 kb on - strandat 500.209 kb on - strandat 500.235 kb on + strandat 500.235 kb on + strandat 500.237 kb on + strandat 500.238 kb on - strandat 500.238 kb on - strandat 500.240 kb on - strandat 500.247 kb on + strandat 500.248 kb on - strandat 500.249 kb on + strandat 500.249 kb on + strandat 500.250 kb on - strandat 500.250 kb on - strandat 500.250 kb on - strandat 500.251 kb on + strandat 500.251 kb on + strandat 500.252 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A
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497,982 + Pf6N2E2_430 0.27 +1.7
497,982 + Pf6N2E2_430 0.27 -0.5
497,983 - Pf6N2E2_430 0.27 +0.5
498,025 + Pf6N2E2_430 0.31 +0.4
498,025 + Pf6N2E2_430 0.31 -0.4
498,026 - Pf6N2E2_430 0.31 +1.4
498,208 + Pf6N2E2_430 0.48 -1.1
498,208 + +3.0
498,208 + Pf6N2E2_430 0.48 -0.7
498,208 + Pf6N2E2_430 0.48 +0.4
498,208 + Pf6N2E2_430 0.48 -0.7
498,208 + Pf6N2E2_430 0.48 -0.0
498,208 + Pf6N2E2_430 0.48 -1.6
498,209 - Pf6N2E2_430 0.48 -0.8
498,209 - Pf6N2E2_430 0.48 -0.5
498,209 - Pf6N2E2_430 0.48 +0.4
498,209 - Pf6N2E2_430 0.48 +1.1
498,209 - Pf6N2E2_430 0.48 -0.3
498,209 - Pf6N2E2_430 0.48 -0.6
498,250 + Pf6N2E2_430 0.52 +0.6
498,532 + Pf6N2E2_430 0.78 -0.4
498,533 - Pf6N2E2_430 0.78 -0.6
498,533 - Pf6N2E2_430 0.78 -0.6
498,609 - Pf6N2E2_430 0.85 -0.2
498,662 + Pf6N2E2_430 0.90 -1.9
498,683 - -0.7
498,809 - -0.2
498,875 - Pf6N2E2_431 0.15 -2.6
498,899 - Pf6N2E2_431 0.18 +1.4
498,899 - Pf6N2E2_431 0.18 -2.2
498,899 - Pf6N2E2_431 0.18 -0.3
499,150 + Pf6N2E2_431 0.55 -2.4
499,150 + Pf6N2E2_431 0.55 -0.2
499,151 - Pf6N2E2_431 0.55 -2.6
499,151 - Pf6N2E2_431 0.55 -1.1
499,151 - Pf6N2E2_431 0.55 +0.0
499,151 - Pf6N2E2_431 0.55 -1.8
499,151 - Pf6N2E2_431 0.55 -2.8
499,151 - Pf6N2E2_431 0.55 +1.4
499,261 + Pf6N2E2_431 0.72 -1.4
499,262 - Pf6N2E2_431 0.72 -0.8
499,262 - Pf6N2E2_431 0.72 -2.0
499,262 - Pf6N2E2_431 0.72 -0.6
499,353 + Pf6N2E2_431 0.85 -4.0
499,354 - Pf6N2E2_431 0.85 +0.4
499,432 + -3.1
499,433 - -3.1
499,457 + -2.8
499,511 + -3.9
499,511 + +0.4
499,512 - -2.8
499,640 + Pf6N2E2_432 0.24 -2.4
499,640 + Pf6N2E2_432 0.24 -1.5
499,640 + Pf6N2E2_432 0.24 -3.4
499,868 + Pf6N2E2_432 0.55 -3.9
499,868 + Pf6N2E2_432 0.55 -0.9
499,868 + Pf6N2E2_432 0.55 +0.4
499,868 + Pf6N2E2_432 0.55 -1.3
499,868 + Pf6N2E2_432 0.55 -2.3
499,869 - Pf6N2E2_432 0.55 +1.4
499,869 - Pf6N2E2_432 0.55 +0.4
499,911 - Pf6N2E2_432 0.60 -3.6
499,911 - Pf6N2E2_432 0.60 -0.9
500,021 + Pf6N2E2_432 0.75 -2.6
500,021 + Pf6N2E2_432 0.75 -1.1
500,021 + Pf6N2E2_432 0.75 -2.5
500,058 + Pf6N2E2_432 0.80 -2.1
500,058 + Pf6N2E2_432 0.80 -2.8
500,160 + -3.3
500,160 + -2.9
500,160 + -2.5
500,161 - -3.5
500,208 + -1.6
500,208 + -2.1
500,208 + -2.3
500,208 + -1.5
500,208 + -2.0
500,208 + +1.4
500,209 - -0.4
500,209 - -0.5
500,209 - -0.9
500,209 - -1.1
500,235 + -1.2
500,235 + +0.0
500,237 + -2.2
500,238 - -0.0
500,238 - +0.7
500,240 - -0.1
500,247 + -1.0
500,248 - -1.3
500,249 + +1.4
500,249 + +0.3
500,250 - -0.0
500,250 - +0.6
500,250 - +1.8
500,251 + -0.8
500,251 + -0.2
500,252 - +1.0

Or see this region's nucleotide sequence