Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4274 and Pf6N2E2_4275 are separated by 69 nucleotides Pf6N2E2_4275 and Pf6N2E2_4276 are separated by 429 nucleotides Pf6N2E2_4276 and Pf6N2E2_4277 are separated by 424 nucleotides
Pf6N2E2_4274 - Sensory box/GGDEF family protein, at 4,972,883 to 4,975,723
_4274
Pf6N2E2_4275 - hypothetical protein, at 4,975,793 to 4,976,107
_4275
Pf6N2E2_4276 - Phosphogluconate repressor HexR, RpiR family, at 4,976,537 to 4,977,403
_4276
Pf6N2E2_4277 - LysR family transcriptional regulator PA5437, at 4,977,828 to 4,978,769
_4277
Position (kb)
4976
4977
4978 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4975.652 kb on + strand at 4975.652 kb on + strand at 4975.653 kb on - strand at 4975.653 kb on - strand at 4975.740 kb on + strand at 4975.795 kb on - strand at 4975.798 kb on + strand at 4975.799 kb on - strand at 4975.802 kb on + strand at 4975.830 kb on - strand, within Pf6N2E2_4275 at 4975.979 kb on + strand, within Pf6N2E2_4275 at 4975.979 kb on + strand, within Pf6N2E2_4275 at 4975.979 kb on + strand, within Pf6N2E2_4275 at 4975.979 kb on + strand, within Pf6N2E2_4275 at 4975.979 kb on + strand, within Pf6N2E2_4275 at 4975.980 kb on - strand, within Pf6N2E2_4275 at 4976.015 kb on - strand, within Pf6N2E2_4275 at 4976.015 kb on - strand, within Pf6N2E2_4275 at 4976.021 kb on + strand, within Pf6N2E2_4275 at 4976.021 kb on + strand, within Pf6N2E2_4275 at 4976.111 kb on - strand at 4976.114 kb on - strand at 4976.239 kb on + strand at 4976.248 kb on + strand at 4976.249 kb on - strand at 4976.261 kb on - strand at 4976.261 kb on - strand at 4976.261 kb on - strand at 4976.261 kb on - strand at 4976.262 kb on + strand at 4976.263 kb on - strand at 4976.263 kb on - strand at 4976.263 kb on - strand at 4976.263 kb on - strand at 4976.263 kb on - strand at 4976.398 kb on - strand at 4976.398 kb on - strand at 4976.398 kb on - strand at 4976.482 kb on + strand at 4976.482 kb on + strand at 4976.484 kb on + strand at 4976.488 kb on + strand at 4976.489 kb on - strand at 4976.499 kb on + strand at 4976.499 kb on + strand at 4976.499 kb on + strand at 4976.499 kb on + strand at 4976.499 kb on + strand at 4976.499 kb on + strand at 4976.500 kb on - strand at 4976.610 kb on + strand at 4976.610 kb on + strand at 4976.610 kb on + strand at 4976.611 kb on - strand at 4976.611 kb on - strand at 4976.611 kb on - strand at 4976.638 kb on + strand, within Pf6N2E2_4276 at 4976.638 kb on + strand, within Pf6N2E2_4276 at 4976.638 kb on + strand, within Pf6N2E2_4276 at 4976.638 kb on + strand, within Pf6N2E2_4276 at 4976.638 kb on + strand, within Pf6N2E2_4276 at 4976.638 kb on + strand, within Pf6N2E2_4276 at 4976.638 kb on + strand, within Pf6N2E2_4276 at 4976.639 kb on - strand, within Pf6N2E2_4276 at 4976.639 kb on - strand, within Pf6N2E2_4276 at 4976.639 kb on - strand, within Pf6N2E2_4276 at 4976.639 kb on - strand, within Pf6N2E2_4276 at 4976.639 kb on - strand, within Pf6N2E2_4276 at 4976.816 kb on + strand, within Pf6N2E2_4276 at 4976.817 kb on - strand, within Pf6N2E2_4276 at 4976.849 kb on - strand, within Pf6N2E2_4276 at 4976.849 kb on - strand, within Pf6N2E2_4276 at 4976.937 kb on - strand, within Pf6N2E2_4276 at 4976.937 kb on - strand, within Pf6N2E2_4276 at 4977.014 kb on + strand, within Pf6N2E2_4276 at 4977.283 kb on - strand, within Pf6N2E2_4276 at 4977.401 kb on + strand at 4977.401 kb on + strand at 4977.402 kb on - strand at 4977.402 kb on - strand at 4977.402 kb on - strand at 4977.402 kb on - strand at 4977.402 kb on - strand at 4977.402 kb on - strand at 4977.446 kb on + strand at 4977.446 kb on + strand at 4977.447 kb on - strand at 4977.447 kb on - strand at 4977.447 kb on - strand at 4977.490 kb on + strand at 4977.490 kb on + strand at 4977.491 kb on - strand at 4977.491 kb on - strand at 4977.491 kb on - strand at 4977.494 kb on + strand at 4977.494 kb on + strand at 4977.494 kb on + strand at 4977.494 kb on + strand at 4977.494 kb on + strand at 4977.494 kb on + strand at 4977.494 kb on + strand at 4977.494 kb on + strand at 4977.495 kb on - strand at 4977.495 kb on - strand at 4977.495 kb on - strand at 4977.495 kb on - strand at 4977.495 kb on - strand at 4977.495 kb on - strand at 4977.495 kb on - strand at 4977.495 kb on - strand at 4977.542 kb on + strand at 4977.543 kb on - strand at 4977.563 kb on + strand at 4977.564 kb on - strand at 4977.645 kb on - strand at 4977.657 kb on + strand at 4977.657 kb on + strand at 4977.657 kb on + strand at 4977.657 kb on + strand at 4977.657 kb on + strand at 4977.657 kb on + strand at 4977.657 kb on + strand at 4977.658 kb on - strand at 4977.658 kb on - strand at 4977.658 kb on - strand at 4977.658 kb on - strand at 4977.658 kb on - strand at 4977.658 kb on - strand at 4977.658 kb on - strand at 4977.658 kb on - strand at 4977.658 kb on - strand at 4977.690 kb on - strand at 4977.690 kb on - strand at 4977.690 kb on - strand at 4977.773 kb on + strand at 4977.774 kb on - strand at 4977.834 kb on - strand at 4978.200 kb on + strand, within Pf6N2E2_4277 at 4978.205 kb on + strand, within Pf6N2E2_4277 at 4978.205 kb on + strand, within Pf6N2E2_4277 at 4978.205 kb on + strand, within Pf6N2E2_4277 at 4978.206 kb on - strand, within Pf6N2E2_4277 at 4978.206 kb on - strand, within Pf6N2E2_4277 at 4978.206 kb on - strand, within Pf6N2E2_4277 at 4978.206 kb on - strand, within Pf6N2E2_4277 at 4978.206 kb on - strand, within Pf6N2E2_4277 at 4978.206 kb on - strand, within Pf6N2E2_4277 at 4978.206 kb on - strand, within Pf6N2E2_4277 at 4978.207 kb on + strand, within Pf6N2E2_4277 at 4978.207 kb on + strand, within Pf6N2E2_4277 at 4978.208 kb on - strand, within Pf6N2E2_4277 at 4978.208 kb on - strand, within Pf6N2E2_4277 at 4978.208 kb on - strand, within Pf6N2E2_4277 at 4978.208 kb on - strand, within Pf6N2E2_4277
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 4,975,652 + +0.4 4,975,652 + +0.4 4,975,653 - +0.2 4,975,653 - -0.1 4,975,740 + -1.4 4,975,795 - -0.5 4,975,798 + -0.7 4,975,799 - +0.2 4,975,802 + +0.8 4,975,830 - Pf6N2E2_4275 0.12 -0.3 4,975,979 + Pf6N2E2_4275 0.59 +0.4 4,975,979 + Pf6N2E2_4275 0.59 -0.5 4,975,979 + Pf6N2E2_4275 0.59 -0.8 4,975,979 + Pf6N2E2_4275 0.59 -0.1 4,975,979 + Pf6N2E2_4275 0.59 +1.0 4,975,980 - Pf6N2E2_4275 0.59 -0.3 4,976,015 - Pf6N2E2_4275 0.70 +0.4 4,976,015 - Pf6N2E2_4275 0.70 -0.1 4,976,021 + Pf6N2E2_4275 0.72 -0.5 4,976,021 + Pf6N2E2_4275 0.72 -0.0 4,976,111 - +0.3 4,976,114 - +0.2 4,976,239 + +0.3 4,976,248 + +1.5 4,976,249 - -0.9 4,976,261 - -0.6 4,976,261 - -0.1 4,976,261 - +0.7 4,976,261 - -0.9 4,976,262 + -0.6 4,976,263 - -0.5 4,976,263 - -0.2 4,976,263 - +0.1 4,976,263 - -1.1 4,976,263 - -0.1 4,976,398 - -0.8 4,976,398 - -0.5 4,976,398 - +0.0 4,976,482 + +0.5 4,976,482 + +0.6 4,976,484 + +0.4 4,976,488 + -1.0 4,976,489 - -1.0 4,976,499 + +0.1 4,976,499 + -0.0 4,976,499 + +0.3 4,976,499 + -0.4 4,976,499 + -0.7 4,976,499 + +1.3 4,976,500 - +1.3 4,976,610 + -0.7 4,976,610 + -2.2 4,976,610 + -0.7 4,976,611 - -1.7 4,976,611 - -1.6 4,976,611 - -1.0 4,976,638 + Pf6N2E2_4276 0.12 -0.8 4,976,638 + Pf6N2E2_4276 0.12 +0.6 4,976,638 + Pf6N2E2_4276 0.12 +0.3 4,976,638 + Pf6N2E2_4276 0.12 -1.3 4,976,638 + Pf6N2E2_4276 0.12 -2.9 4,976,638 + Pf6N2E2_4276 0.12 -2.3 4,976,638 + Pf6N2E2_4276 0.12 +1.3 4,976,639 - Pf6N2E2_4276 0.12 -0.8 4,976,639 - Pf6N2E2_4276 0.12 -0.7 4,976,639 - Pf6N2E2_4276 0.12 -1.0 4,976,639 - Pf6N2E2_4276 0.12 -1.9 4,976,639 - Pf6N2E2_4276 0.12 -2.5 4,976,816 + Pf6N2E2_4276 0.32 +0.3 4,976,817 - Pf6N2E2_4276 0.32 +1.3 4,976,849 - Pf6N2E2_4276 0.36 -1.0 4,976,849 - Pf6N2E2_4276 0.36 +1.3 4,976,937 - Pf6N2E2_4276 0.46 -0.8 4,976,937 - Pf6N2E2_4276 0.46 -0.7 4,977,014 + Pf6N2E2_4276 0.55 -0.7 4,977,283 - Pf6N2E2_4276 0.86 -0.1 4,977,401 + -0.3 4,977,401 + -0.4 4,977,402 - -0.9 4,977,402 - -0.4 4,977,402 - +0.8 4,977,402 - -0.1 4,977,402 - -0.5 4,977,402 - -0.6 4,977,446 + -0.4 4,977,446 + +0.3 4,977,447 - -1.1 4,977,447 - +0.7 4,977,447 - -0.3 4,977,490 + +0.6 4,977,490 + +1.3 4,977,491 - +0.0 4,977,491 - +0.4 4,977,491 - -2.0 4,977,494 + +0.2 4,977,494 + -1.2 4,977,494 + -0.8 4,977,494 + +0.2 4,977,494 + +1.9 4,977,494 + -0.7 4,977,494 + -1.5 4,977,494 + -0.5 4,977,495 - -0.2 4,977,495 - +0.3 4,977,495 - +0.4 4,977,495 - -0.9 4,977,495 - -0.2 4,977,495 - +0.1 4,977,495 - +0.3 4,977,495 - +0.3 4,977,542 + -1.3 4,977,543 - +0.5 4,977,563 + +0.5 4,977,564 - +0.5 4,977,645 - -0.2 4,977,657 + +0.7 4,977,657 + +1.3 4,977,657 + -2.2 4,977,657 + +0.5 4,977,657 + -1.2 4,977,657 + +0.4 4,977,657 + +0.5 4,977,658 - -0.2 4,977,658 - -0.3 4,977,658 - -0.3 4,977,658 - -1.0 4,977,658 - +0.5 4,977,658 - +0.2 4,977,658 - +0.2 4,977,658 - -0.5 4,977,658 - +0.0 4,977,690 - -0.4 4,977,690 - +0.0 4,977,690 - +0.1 4,977,773 + -0.7 4,977,774 - -0.3 4,977,834 - +2.3 4,978,200 + Pf6N2E2_4277 0.39 -1.4 4,978,205 + Pf6N2E2_4277 0.40 -0.0 4,978,205 + Pf6N2E2_4277 0.40 -0.5 4,978,205 + Pf6N2E2_4277 0.40 -0.1 4,978,206 - Pf6N2E2_4277 0.40 +0.7 4,978,206 - Pf6N2E2_4277 0.40 -0.2 4,978,206 - Pf6N2E2_4277 0.40 -1.1 4,978,206 - Pf6N2E2_4277 0.40 -0.6 4,978,206 - Pf6N2E2_4277 0.40 -0.3 4,978,206 - Pf6N2E2_4277 0.40 -2.2 4,978,206 - Pf6N2E2_4277 0.40 -2.1 4,978,207 + Pf6N2E2_4277 0.40 -1.2 4,978,207 + Pf6N2E2_4277 0.40 +1.5 4,978,208 - Pf6N2E2_4277 0.40 -0.5 4,978,208 - Pf6N2E2_4277 0.40 +0.2 4,978,208 - Pf6N2E2_4277 0.40 -0.6 4,978,208 - Pf6N2E2_4277 0.40 -0.9
Or see this region's nucleotide sequence