Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4127 and Pf6N2E2_4128 are separated by 35 nucleotides Pf6N2E2_4128 and Pf6N2E2_4129 are separated by 9 nucleotides Pf6N2E2_4129 and Pf6N2E2_4130 are separated by 13 nucleotides Pf6N2E2_4130 and Pf6N2E2_4131 are separated by 115 nucleotides
Pf6N2E2_4127 - Quinone oxidoreductase (EC 1.6.5.5), at 4,806,280 to 4,807,257
_4127
Pf6N2E2_4128 - hypothetical protein, at 4,807,293 to 4,807,577
_4128
Pf6N2E2_4129 - Coproporphyrinogen III oxidase, aerobic (EC 1.3.3.3), at 4,807,587 to 4,808,501
_4129
Pf6N2E2_4130 - Shikimate 5-dehydrogenase I alpha (EC 1.1.1.25), at 4,808,515 to 4,809,336
_4130
Pf6N2E2_4131 - Sulfate permease, at 4,809,452 to 4,811,020
_4131
Position (kb)
4807
4808
4809 Strain fitness (log2 ratio)
-1
0
1 at 4806.617 kb on + strand, within Pf6N2E2_4127 at 4806.683 kb on + strand, within Pf6N2E2_4127 at 4806.683 kb on + strand, within Pf6N2E2_4127 at 4806.684 kb on - strand, within Pf6N2E2_4127 at 4806.684 kb on - strand, within Pf6N2E2_4127 at 4806.851 kb on + strand, within Pf6N2E2_4127 at 4806.851 kb on + strand, within Pf6N2E2_4127 at 4806.851 kb on + strand, within Pf6N2E2_4127 at 4806.851 kb on + strand, within Pf6N2E2_4127 at 4806.851 kb on + strand, within Pf6N2E2_4127 at 4806.851 kb on + strand, within Pf6N2E2_4127 at 4806.852 kb on - strand, within Pf6N2E2_4127 at 4806.852 kb on - strand, within Pf6N2E2_4127 at 4806.852 kb on - strand, within Pf6N2E2_4127 at 4806.852 kb on - strand, within Pf6N2E2_4127 at 4806.869 kb on + strand, within Pf6N2E2_4127 at 4806.869 kb on + strand, within Pf6N2E2_4127 at 4806.869 kb on + strand, within Pf6N2E2_4127 at 4806.870 kb on - strand, within Pf6N2E2_4127 at 4806.955 kb on + strand, within Pf6N2E2_4127 at 4806.974 kb on + strand, within Pf6N2E2_4127 at 4806.974 kb on + strand, within Pf6N2E2_4127 at 4806.975 kb on - strand, within Pf6N2E2_4127 at 4807.119 kb on - strand, within Pf6N2E2_4127 at 4807.121 kb on + strand, within Pf6N2E2_4127 at 4807.121 kb on + strand, within Pf6N2E2_4127 at 4807.122 kb on - strand, within Pf6N2E2_4127 at 4807.122 kb on - strand, within Pf6N2E2_4127 at 4807.122 kb on - strand, within Pf6N2E2_4127 at 4807.122 kb on - strand, within Pf6N2E2_4127 at 4807.196 kb on + strand at 4807.205 kb on + strand at 4807.206 kb on - strand at 4807.206 kb on - strand at 4807.206 kb on - strand at 4807.286 kb on + strand at 4807.286 kb on + strand at 4807.286 kb on + strand at 4807.286 kb on + strand at 4807.286 kb on + strand at 4807.287 kb on - strand at 4807.287 kb on - strand at 4807.294 kb on + strand at 4807.295 kb on - strand at 4807.295 kb on - strand at 4807.295 kb on - strand at 4807.452 kb on + strand, within Pf6N2E2_4128 at 4807.453 kb on - strand, within Pf6N2E2_4128 at 4807.453 kb on - strand, within Pf6N2E2_4128 at 4807.453 kb on - strand, within Pf6N2E2_4128 at 4807.475 kb on + strand, within Pf6N2E2_4128 at 4807.518 kb on + strand, within Pf6N2E2_4128 at 4807.518 kb on + strand, within Pf6N2E2_4128 at 4807.565 kb on + strand at 4808.524 kb on + strand at 4809.385 kb on - strand at 4809.389 kb on + strand at 4809.389 kb on + strand at 4809.453 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 4,806,617 + Pf6N2E2_4127 0.34 -0.1 4,806,683 + Pf6N2E2_4127 0.41 -0.3 4,806,683 + Pf6N2E2_4127 0.41 +0.3 4,806,684 - Pf6N2E2_4127 0.41 -1.0 4,806,684 - Pf6N2E2_4127 0.41 -0.2 4,806,851 + Pf6N2E2_4127 0.58 +0.4 4,806,851 + Pf6N2E2_4127 0.58 -1.5 4,806,851 + Pf6N2E2_4127 0.58 -0.1 4,806,851 + Pf6N2E2_4127 0.58 -0.8 4,806,851 + Pf6N2E2_4127 0.58 -0.8 4,806,851 + Pf6N2E2_4127 0.58 -0.1 4,806,852 - Pf6N2E2_4127 0.58 -1.5 4,806,852 - Pf6N2E2_4127 0.58 -0.2 4,806,852 - Pf6N2E2_4127 0.58 +0.0 4,806,852 - Pf6N2E2_4127 0.58 +0.1 4,806,869 + Pf6N2E2_4127 0.60 -0.2 4,806,869 + Pf6N2E2_4127 0.60 +0.8 4,806,869 + Pf6N2E2_4127 0.60 -0.4 4,806,870 - Pf6N2E2_4127 0.60 +0.3 4,806,955 + Pf6N2E2_4127 0.69 -1.1 4,806,974 + Pf6N2E2_4127 0.71 +1.3 4,806,974 + Pf6N2E2_4127 0.71 +0.6 4,806,975 - Pf6N2E2_4127 0.71 +0.9 4,807,119 - Pf6N2E2_4127 0.86 -0.2 4,807,121 + Pf6N2E2_4127 0.86 -0.4 4,807,121 + Pf6N2E2_4127 0.86 -0.9 4,807,122 - Pf6N2E2_4127 0.86 -0.7 4,807,122 - Pf6N2E2_4127 0.86 -0.4 4,807,122 - Pf6N2E2_4127 0.86 -0.4 4,807,122 - Pf6N2E2_4127 0.86 +0.3 4,807,196 + -0.0 4,807,205 + +0.3 4,807,206 - +0.0 4,807,206 - -0.7 4,807,206 - -0.5 4,807,286 + +0.3 4,807,286 + -0.0 4,807,286 + -0.4 4,807,286 + +1.3 4,807,286 + -0.2 4,807,287 - -0.3 4,807,287 - -0.2 4,807,294 + +0.6 4,807,295 - +0.3 4,807,295 - +0.4 4,807,295 - +0.7 4,807,452 + Pf6N2E2_4128 0.56 +0.4 4,807,453 - Pf6N2E2_4128 0.56 +0.3 4,807,453 - Pf6N2E2_4128 0.56 -1.1 4,807,453 - Pf6N2E2_4128 0.56 -1.7 4,807,475 + Pf6N2E2_4128 0.64 -1.0 4,807,518 + Pf6N2E2_4128 0.79 -0.8 4,807,518 + Pf6N2E2_4128 0.79 -0.1 4,807,565 + -1.2 4,808,524 + +1.2 4,809,385 - -0.3 4,809,389 + -0.8 4,809,389 + -1.0 4,809,453 + +0.3
Or see this region's nucleotide sequence