Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4046 and Pf6N2E2_4047 are separated by 84 nucleotides Pf6N2E2_4047 and Pf6N2E2_4048 overlap by 1 nucleotides
Pf6N2E2_4046 - FIG00953482: hypothetical protein, at 4,726,072 to 4,728,033
_4046
Pf6N2E2_4047 - Tryptophan synthase alpha chain (EC 4.2.1.20), at 4,728,118 to 4,728,930
_4047
Pf6N2E2_4048 - Tryptophan synthase beta chain (EC 4.2.1.20), at 4,728,930 to 4,730,162
_4048
Position (kb)
4728
4729 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 4727.119 kb on + strand, within Pf6N2E2_4046 at 4727.119 kb on + strand, within Pf6N2E2_4046 at 4727.119 kb on + strand, within Pf6N2E2_4046 at 4727.120 kb on - strand, within Pf6N2E2_4046 at 4727.138 kb on + strand, within Pf6N2E2_4046 at 4727.138 kb on + strand, within Pf6N2E2_4046 at 4727.139 kb on - strand, within Pf6N2E2_4046 at 4727.139 kb on - strand, within Pf6N2E2_4046 at 4727.167 kb on + strand, within Pf6N2E2_4046 at 4727.167 kb on + strand, within Pf6N2E2_4046 at 4727.167 kb on + strand, within Pf6N2E2_4046 at 4727.168 kb on - strand, within Pf6N2E2_4046 at 4727.168 kb on - strand, within Pf6N2E2_4046 at 4727.168 kb on - strand, within Pf6N2E2_4046 at 4727.269 kb on + strand, within Pf6N2E2_4046 at 4727.269 kb on + strand, within Pf6N2E2_4046 at 4727.281 kb on + strand, within Pf6N2E2_4046 at 4727.281 kb on + strand, within Pf6N2E2_4046 at 4727.281 kb on + strand, within Pf6N2E2_4046 at 4727.281 kb on + strand, within Pf6N2E2_4046 at 4727.281 kb on + strand, within Pf6N2E2_4046 at 4727.282 kb on - strand, within Pf6N2E2_4046 at 4727.282 kb on - strand, within Pf6N2E2_4046 at 4727.282 kb on - strand, within Pf6N2E2_4046 at 4727.282 kb on - strand, within Pf6N2E2_4046 at 4727.282 kb on - strand, within Pf6N2E2_4046 at 4727.282 kb on - strand, within Pf6N2E2_4046 at 4727.284 kb on + strand, within Pf6N2E2_4046 at 4727.284 kb on + strand, within Pf6N2E2_4046 at 4727.284 kb on + strand, within Pf6N2E2_4046 at 4727.284 kb on + strand, within Pf6N2E2_4046 at 4727.284 kb on + strand, within Pf6N2E2_4046 at 4727.284 kb on + strand, within Pf6N2E2_4046 at 4727.284 kb on + strand, within Pf6N2E2_4046 at 4727.284 kb on + strand, within Pf6N2E2_4046 at 4727.285 kb on - strand, within Pf6N2E2_4046 at 4727.285 kb on - strand, within Pf6N2E2_4046 at 4727.285 kb on - strand, within Pf6N2E2_4046 at 4727.285 kb on - strand, within Pf6N2E2_4046 at 4727.285 kb on - strand, within Pf6N2E2_4046 at 4727.298 kb on + strand, within Pf6N2E2_4046 at 4727.298 kb on + strand, within Pf6N2E2_4046 at 4727.298 kb on + strand, within Pf6N2E2_4046 at 4727.299 kb on - strand, within Pf6N2E2_4046 at 4727.299 kb on - strand, within Pf6N2E2_4046 at 4727.521 kb on + strand, within Pf6N2E2_4046 at 4727.521 kb on + strand, within Pf6N2E2_4046 at 4727.522 kb on - strand, within Pf6N2E2_4046 at 4727.522 kb on - strand, within Pf6N2E2_4046 at 4728.053 kb on + strand at 4728.054 kb on - strand at 4728.054 kb on - strand at 4728.405 kb on - strand, within Pf6N2E2_4047 at 4728.405 kb on - strand, within Pf6N2E2_4047 at 4728.405 kb on - strand, within Pf6N2E2_4047 at 4728.405 kb on - strand, within Pf6N2E2_4047 at 4728.405 kb on - strand, within Pf6N2E2_4047 at 4728.405 kb on - strand, within Pf6N2E2_4047 at 4728.405 kb on - strand, within Pf6N2E2_4047 at 4728.405 kb on - strand, within Pf6N2E2_4047 at 4728.408 kb on - strand, within Pf6N2E2_4047 at 4728.408 kb on - strand, within Pf6N2E2_4047 at 4728.408 kb on - strand, within Pf6N2E2_4047 at 4728.409 kb on + strand, within Pf6N2E2_4047 at 4728.410 kb on - strand, within Pf6N2E2_4047 at 4728.410 kb on - strand, within Pf6N2E2_4047 at 4728.410 kb on - strand, within Pf6N2E2_4047 at 4728.410 kb on - strand, within Pf6N2E2_4047 at 4728.410 kb on - strand, within Pf6N2E2_4047 at 4728.410 kb on - strand, within Pf6N2E2_4047 at 4728.410 kb on - strand, within Pf6N2E2_4047 at 4728.410 kb on - strand, within Pf6N2E2_4047 at 4728.410 kb on - strand, within Pf6N2E2_4047 at 4728.627 kb on - strand, within Pf6N2E2_4047 at 4728.627 kb on - strand, within Pf6N2E2_4047 at 4729.157 kb on - strand, within Pf6N2E2_4048 at 4729.157 kb on - strand, within Pf6N2E2_4048 at 4729.294 kb on - strand, within Pf6N2E2_4048 at 4729.294 kb on - strand, within Pf6N2E2_4048 at 4729.571 kb on - strand, within Pf6N2E2_4048 at 4729.571 kb on - strand, within Pf6N2E2_4048 at 4729.571 kb on - strand, within Pf6N2E2_4048 at 4729.571 kb on - strand, within Pf6N2E2_4048 at 4729.730 kb on - strand, within Pf6N2E2_4048 at 4729.730 kb on - strand, within Pf6N2E2_4048 at 4729.730 kb on - strand, within Pf6N2E2_4048 at 4729.730 kb on - strand, within Pf6N2E2_4048 at 4729.730 kb on - strand, within Pf6N2E2_4048 at 4729.907 kb on - strand, within Pf6N2E2_4048 at 4729.907 kb on - strand, within Pf6N2E2_4048 at 4729.907 kb on - strand, within Pf6N2E2_4048 at 4729.907 kb on - strand, within Pf6N2E2_4048 at 4729.907 kb on - strand, within Pf6N2E2_4048 at 4729.907 kb on - strand, within Pf6N2E2_4048 at 4729.928 kb on - strand, within Pf6N2E2_4048
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 4,727,119 + Pf6N2E2_4046 0.53 +0.1 4,727,119 + Pf6N2E2_4046 0.53 +0.2 4,727,119 + Pf6N2E2_4046 0.53 -0.3 4,727,120 - Pf6N2E2_4046 0.53 -0.2 4,727,138 + Pf6N2E2_4046 0.54 +1.3 4,727,138 + Pf6N2E2_4046 0.54 -0.7 4,727,139 - Pf6N2E2_4046 0.54 +0.1 4,727,139 - Pf6N2E2_4046 0.54 -0.9 4,727,167 + Pf6N2E2_4046 0.56 +0.2 4,727,167 + Pf6N2E2_4046 0.56 -0.6 4,727,167 + Pf6N2E2_4046 0.56 +0.3 4,727,168 - Pf6N2E2_4046 0.56 -0.9 4,727,168 - Pf6N2E2_4046 0.56 -0.4 4,727,168 - Pf6N2E2_4046 0.56 -0.6 4,727,269 + Pf6N2E2_4046 0.61 -0.2 4,727,269 + Pf6N2E2_4046 0.61 -0.2 4,727,281 + Pf6N2E2_4046 0.62 +0.0 4,727,281 + Pf6N2E2_4046 0.62 +0.5 4,727,281 + Pf6N2E2_4046 0.62 +0.2 4,727,281 + Pf6N2E2_4046 0.62 +0.9 4,727,281 + Pf6N2E2_4046 0.62 -0.1 4,727,282 - Pf6N2E2_4046 0.62 -0.4 4,727,282 - Pf6N2E2_4046 0.62 -1.0 4,727,282 - Pf6N2E2_4046 0.62 -0.7 4,727,282 - Pf6N2E2_4046 0.62 -0.6 4,727,282 - Pf6N2E2_4046 0.62 -0.1 4,727,282 - Pf6N2E2_4046 0.62 -1.0 4,727,284 + Pf6N2E2_4046 0.62 -1.2 4,727,284 + Pf6N2E2_4046 0.62 -1.9 4,727,284 + Pf6N2E2_4046 0.62 -0.1 4,727,284 + Pf6N2E2_4046 0.62 -0.8 4,727,284 + Pf6N2E2_4046 0.62 +0.8 4,727,284 + Pf6N2E2_4046 0.62 -0.5 4,727,284 + Pf6N2E2_4046 0.62 +0.4 4,727,284 + Pf6N2E2_4046 0.62 -0.1 4,727,285 - Pf6N2E2_4046 0.62 -0.7 4,727,285 - Pf6N2E2_4046 0.62 +1.3 4,727,285 - Pf6N2E2_4046 0.62 +0.8 4,727,285 - Pf6N2E2_4046 0.62 +0.2 4,727,285 - Pf6N2E2_4046 0.62 +1.3 4,727,298 + Pf6N2E2_4046 0.62 +0.5 4,727,298 + Pf6N2E2_4046 0.62 -0.5 4,727,298 + Pf6N2E2_4046 0.62 +1.0 4,727,299 - Pf6N2E2_4046 0.63 +0.5 4,727,299 - Pf6N2E2_4046 0.63 +0.3 4,727,521 + Pf6N2E2_4046 0.74 -0.0 4,727,521 + Pf6N2E2_4046 0.74 -0.3 4,727,522 - Pf6N2E2_4046 0.74 -0.8 4,727,522 - Pf6N2E2_4046 0.74 -0.5 4,728,053 + -0.2 4,728,054 - +0.3 4,728,054 - -1.2 4,728,405 - Pf6N2E2_4047 0.35 +0.3 4,728,405 - Pf6N2E2_4047 0.35 -1.7 4,728,405 - Pf6N2E2_4047 0.35 -3.5 4,728,405 - Pf6N2E2_4047 0.35 +1.3 4,728,405 - Pf6N2E2_4047 0.35 +2.3 4,728,405 - Pf6N2E2_4047 0.35 -2.6 4,728,405 - Pf6N2E2_4047 0.35 +0.3 4,728,405 - Pf6N2E2_4047 0.35 -0.2 4,728,408 - Pf6N2E2_4047 0.36 -2.1 4,728,408 - Pf6N2E2_4047 0.36 -2.2 4,728,408 - Pf6N2E2_4047 0.36 -0.7 4,728,409 + Pf6N2E2_4047 0.36 -0.7 4,728,410 - Pf6N2E2_4047 0.36 -2.4 4,728,410 - Pf6N2E2_4047 0.36 -1.7 4,728,410 - Pf6N2E2_4047 0.36 -3.7 4,728,410 - Pf6N2E2_4047 0.36 -3.2 4,728,410 - Pf6N2E2_4047 0.36 +1.3 4,728,410 - Pf6N2E2_4047 0.36 -3.2 4,728,410 - Pf6N2E2_4047 0.36 -2.4 4,728,410 - Pf6N2E2_4047 0.36 -2.9 4,728,410 - Pf6N2E2_4047 0.36 -1.7 4,728,627 - Pf6N2E2_4047 0.63 -2.5 4,728,627 - Pf6N2E2_4047 0.63 -0.7 4,729,157 - Pf6N2E2_4048 0.18 -1.0 4,729,157 - Pf6N2E2_4048 0.18 -1.0 4,729,294 - Pf6N2E2_4048 0.30 -2.2 4,729,294 - Pf6N2E2_4048 0.30 -1.0 4,729,571 - Pf6N2E2_4048 0.52 -1.8 4,729,571 - Pf6N2E2_4048 0.52 -0.2 4,729,571 - Pf6N2E2_4048 0.52 +0.3 4,729,571 - Pf6N2E2_4048 0.52 -2.7 4,729,730 - Pf6N2E2_4048 0.65 +0.3 4,729,730 - Pf6N2E2_4048 0.65 -2.7 4,729,730 - Pf6N2E2_4048 0.65 -1.8 4,729,730 - Pf6N2E2_4048 0.65 -5.5 4,729,730 - Pf6N2E2_4048 0.65 -3.3 4,729,907 - Pf6N2E2_4048 0.79 -3.1 4,729,907 - Pf6N2E2_4048 0.79 -3.9 4,729,907 - Pf6N2E2_4048 0.79 -3.7 4,729,907 - Pf6N2E2_4048 0.79 +1.3 4,729,907 - Pf6N2E2_4048 0.79 -4.4 4,729,907 - Pf6N2E2_4048 0.79 -2.4 4,729,928 - Pf6N2E2_4048 0.81 -3.1
Or see this region's nucleotide sequence