Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3837 and Pf6N2E2_3838 are separated by 63 nucleotides Pf6N2E2_3838 and Pf6N2E2_3839 are separated by 9 nucleotides Pf6N2E2_3839 and Pf6N2E2_3840 are separated by 42 nucleotides Pf6N2E2_3840 and Pf6N2E2_3841 overlap by 1 nucleotides Pf6N2E2_3841 and Pf6N2E2_3842 overlap by 1 nucleotides
Pf6N2E2_3837 - ABC-type amino acid transport, signal transduction systems, periplasmic component/domain, at 4,470,580 to 4,471,326
_3837
Pf6N2E2_3838 - Imidazole glycerol phosphate synthase cyclase subunit (EC 4.1.3.-), at 4,471,390 to 4,472,160
_3838
Pf6N2E2_3839 - 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data) , at 4,472,170 to 4,472,907
_3839
Pf6N2E2_3840 - FIG00956267: hypothetical protein, at 4,472,950 to 4,473,204
_3840
Pf6N2E2_3841 - Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-), at 4,473,204 to 4,473,842
_3841
Pf6N2E2_3842 - Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19), at 4,473,842 to 4,474,435
_3842
Position (kb)
4472
4473 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4471.217 kb on + strand, within Pf6N2E2_3837 at 4471.217 kb on + strand, within Pf6N2E2_3837 at 4471.217 kb on + strand, within Pf6N2E2_3837 at 4471.217 kb on + strand, within Pf6N2E2_3837 at 4471.217 kb on + strand, within Pf6N2E2_3837 at 4471.218 kb on - strand, within Pf6N2E2_3837 at 4471.218 kb on - strand, within Pf6N2E2_3837 at 4471.218 kb on - strand, within Pf6N2E2_3837 at 4471.218 kb on - strand, within Pf6N2E2_3837 at 4471.218 kb on - strand, within Pf6N2E2_3837 at 4471.218 kb on - strand, within Pf6N2E2_3837 at 4471.218 kb on - strand, within Pf6N2E2_3837 at 4471.218 kb on - strand, within Pf6N2E2_3837 at 4471.254 kb on + strand at 4471.254 kb on + strand at 4471.255 kb on - strand at 4471.255 kb on - strand at 4471.377 kb on + strand at 4471.377 kb on + strand at 4471.378 kb on - strand at 4471.378 kb on - strand at 4471.378 kb on - strand at 4471.421 kb on + strand at 4471.422 kb on - strand at 4471.422 kb on - strand at 4472.180 kb on + strand at 4472.181 kb on - strand at 4472.181 kb on - strand at 4472.228 kb on + strand at 4472.228 kb on + strand at 4472.229 kb on - strand at 4472.411 kb on + strand, within Pf6N2E2_3839 at 4472.412 kb on - strand, within Pf6N2E2_3839 at 4472.412 kb on - strand, within Pf6N2E2_3839 at 4472.609 kb on + strand, within Pf6N2E2_3839 at 4472.630 kb on + strand, within Pf6N2E2_3839 at 4472.630 kb on + strand, within Pf6N2E2_3839 at 4472.630 kb on + strand, within Pf6N2E2_3839 at 4472.631 kb on - strand, within Pf6N2E2_3839 at 4472.714 kb on - strand, within Pf6N2E2_3839 at 4472.714 kb on - strand, within Pf6N2E2_3839 at 4472.752 kb on - strand, within Pf6N2E2_3839 at 4472.864 kb on - strand at 4472.864 kb on - strand at 4472.890 kb on - strand at 4472.924 kb on + strand at 4472.925 kb on - strand at 4472.925 kb on - strand at 4472.925 kb on - strand at 4472.930 kb on + strand at 4473.003 kb on + strand, within Pf6N2E2_3840 at 4473.004 kb on - strand, within Pf6N2E2_3840 at 4473.004 kb on - strand, within Pf6N2E2_3840 at 4473.038 kb on + strand, within Pf6N2E2_3840 at 4473.039 kb on - strand, within Pf6N2E2_3840 at 4473.039 kb on - strand, within Pf6N2E2_3840 at 4473.039 kb on - strand, within Pf6N2E2_3840 at 4473.039 kb on - strand, within Pf6N2E2_3840 at 4473.039 kb on - strand, within Pf6N2E2_3840 at 4473.039 kb on - strand, within Pf6N2E2_3840 at 4473.041 kb on + strand, within Pf6N2E2_3840 at 4473.041 kb on + strand, within Pf6N2E2_3840 at 4473.041 kb on + strand, within Pf6N2E2_3840 at 4473.332 kb on - strand, within Pf6N2E2_3841 at 4473.332 kb on - strand, within Pf6N2E2_3841 at 4473.376 kb on + strand, within Pf6N2E2_3841 at 4473.377 kb on - strand, within Pf6N2E2_3841 at 4473.377 kb on - strand, within Pf6N2E2_3841 at 4473.377 kb on - strand, within Pf6N2E2_3841 at 4473.377 kb on - strand, within Pf6N2E2_3841 at 4473.379 kb on + strand, within Pf6N2E2_3841 at 4473.379 kb on + strand, within Pf6N2E2_3841 at 4473.380 kb on - strand, within Pf6N2E2_3841 at 4473.380 kb on - strand, within Pf6N2E2_3841 at 4473.380 kb on - strand, within Pf6N2E2_3841 at 4473.380 kb on - strand, within Pf6N2E2_3841 at 4473.394 kb on + strand, within Pf6N2E2_3841 at 4473.395 kb on - strand, within Pf6N2E2_3841 at 4473.606 kb on - strand, within Pf6N2E2_3841 at 4473.606 kb on - strand, within Pf6N2E2_3841 at 4473.844 kb on - strand at 4473.859 kb on + strand at 4473.859 kb on + strand at 4473.860 kb on - strand at 4473.860 kb on - strand at 4473.860 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 4,471,217 + Pf6N2E2_3837 0.85 +1.3 4,471,217 + Pf6N2E2_3837 0.85 -0.1 4,471,217 + Pf6N2E2_3837 0.85 -0.4 4,471,217 + Pf6N2E2_3837 0.85 +0.1 4,471,217 + Pf6N2E2_3837 0.85 +0.1 4,471,218 - Pf6N2E2_3837 0.85 -0.1 4,471,218 - Pf6N2E2_3837 0.85 -1.0 4,471,218 - Pf6N2E2_3837 0.85 -0.9 4,471,218 - Pf6N2E2_3837 0.85 -1.0 4,471,218 - Pf6N2E2_3837 0.85 -0.4 4,471,218 - Pf6N2E2_3837 0.85 +0.8 4,471,218 - Pf6N2E2_3837 0.85 -0.3 4,471,218 - Pf6N2E2_3837 0.85 +0.1 4,471,254 + +0.1 4,471,254 + +0.4 4,471,255 - +0.5 4,471,255 - +0.2 4,471,377 + -0.1 4,471,377 + +0.2 4,471,378 - -0.2 4,471,378 - +1.3 4,471,378 - +0.3 4,471,421 + -0.3 4,471,422 - -0.7 4,471,422 - -0.3 4,472,180 + -0.6 4,472,181 - +0.3 4,472,181 - +0.4 4,472,228 + -2.6 4,472,228 + -0.8 4,472,229 - -2.0 4,472,411 + Pf6N2E2_3839 0.33 -3.3 4,472,412 - Pf6N2E2_3839 0.33 -3.3 4,472,412 - Pf6N2E2_3839 0.33 -3.6 4,472,609 + Pf6N2E2_3839 0.59 -3.2 4,472,630 + Pf6N2E2_3839 0.62 +0.3 4,472,630 + Pf6N2E2_3839 0.62 -1.9 4,472,630 + Pf6N2E2_3839 0.62 -0.1 4,472,631 - Pf6N2E2_3839 0.62 -2.5 4,472,714 - Pf6N2E2_3839 0.74 -3.6 4,472,714 - Pf6N2E2_3839 0.74 -2.3 4,472,752 - Pf6N2E2_3839 0.79 -3.1 4,472,864 - -1.9 4,472,864 - -4.0 4,472,890 - -1.8 4,472,924 + -1.3 4,472,925 - -0.5 4,472,925 - -0.0 4,472,925 - -1.2 4,472,930 + -4.2 4,473,003 + Pf6N2E2_3840 0.21 -2.0 4,473,004 - Pf6N2E2_3840 0.21 -0.1 4,473,004 - Pf6N2E2_3840 0.21 +0.4 4,473,038 + Pf6N2E2_3840 0.35 -0.3 4,473,039 - Pf6N2E2_3840 0.35 -0.1 4,473,039 - Pf6N2E2_3840 0.35 -0.3 4,473,039 - Pf6N2E2_3840 0.35 +0.2 4,473,039 - Pf6N2E2_3840 0.35 +0.6 4,473,039 - Pf6N2E2_3840 0.35 -0.5 4,473,039 - Pf6N2E2_3840 0.35 -1.7 4,473,041 + Pf6N2E2_3840 0.36 -1.5 4,473,041 + Pf6N2E2_3840 0.36 -2.7 4,473,041 + Pf6N2E2_3840 0.36 -2.0 4,473,332 - Pf6N2E2_3841 0.20 -2.8 4,473,332 - Pf6N2E2_3841 0.20 -3.1 4,473,376 + Pf6N2E2_3841 0.27 +1.3 4,473,377 - Pf6N2E2_3841 0.27 -0.3 4,473,377 - Pf6N2E2_3841 0.27 -2.4 4,473,377 - Pf6N2E2_3841 0.27 -1.5 4,473,377 - Pf6N2E2_3841 0.27 -3.2 4,473,379 + Pf6N2E2_3841 0.27 +1.3 4,473,379 + Pf6N2E2_3841 0.27 +1.3 4,473,380 - Pf6N2E2_3841 0.28 +1.3 4,473,380 - Pf6N2E2_3841 0.28 -1.9 4,473,380 - Pf6N2E2_3841 0.28 -3.4 4,473,380 - Pf6N2E2_3841 0.28 -3.3 4,473,394 + Pf6N2E2_3841 0.30 -1.5 4,473,395 - Pf6N2E2_3841 0.30 -1.7 4,473,606 - Pf6N2E2_3841 0.63 -3.2 4,473,606 - Pf6N2E2_3841 0.63 -2.9 4,473,844 - -3.2 4,473,859 + +0.3 4,473,859 + +0.3 4,473,860 - -3.2 4,473,860 - -0.1 4,473,860 - -3.5
Or see this region's nucleotide sequence