Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3749 and Pf6N2E2_3750 are separated by 341 nucleotides Pf6N2E2_3750 and Pf6N2E2_3751 are separated by 220 nucleotides
Pf6N2E2_3749 - Chitin binding protein, at 4,363,903 to 4,365,366
_3749
Pf6N2E2_3750 - Uroporphyrinogen III decarboxylase (EC 4.1.1.37), at 4,365,708 to 4,366,775
_3750
Pf6N2E2_3751 - Glutamate synthase [NADPH] small chain (EC 1.4.1.13), at 4,366,996 to 4,368,414
_3751
Position (kb)
4365
4366
4367 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 4364.765 kb on + strand, within Pf6N2E2_3749 at 4364.765 kb on + strand, within Pf6N2E2_3749 at 4364.766 kb on - strand, within Pf6N2E2_3749 at 4364.826 kb on + strand, within Pf6N2E2_3749 at 4364.826 kb on + strand, within Pf6N2E2_3749 at 4364.827 kb on - strand, within Pf6N2E2_3749 at 4364.827 kb on - strand, within Pf6N2E2_3749 at 4364.892 kb on + strand, within Pf6N2E2_3749 at 4364.966 kb on + strand, within Pf6N2E2_3749 at 4364.967 kb on - strand, within Pf6N2E2_3749 at 4364.967 kb on - strand, within Pf6N2E2_3749 at 4364.967 kb on - strand, within Pf6N2E2_3749 at 4364.967 kb on - strand, within Pf6N2E2_3749 at 4364.999 kb on + strand, within Pf6N2E2_3749 at 4364.999 kb on + strand, within Pf6N2E2_3749 at 4364.999 kb on + strand, within Pf6N2E2_3749 at 4365.000 kb on - strand, within Pf6N2E2_3749 at 4365.000 kb on - strand, within Pf6N2E2_3749 at 4365.180 kb on + strand, within Pf6N2E2_3749 at 4365.180 kb on + strand, within Pf6N2E2_3749 at 4365.180 kb on + strand, within Pf6N2E2_3749 at 4365.180 kb on + strand, within Pf6N2E2_3749 at 4365.181 kb on - strand, within Pf6N2E2_3749 at 4365.191 kb on + strand, within Pf6N2E2_3749 at 4365.211 kb on + strand, within Pf6N2E2_3749 at 4365.337 kb on + strand at 4365.337 kb on + strand at 4365.337 kb on + strand at 4365.337 kb on + strand at 4365.338 kb on - strand at 4365.338 kb on - strand at 4365.338 kb on - strand at 4365.338 kb on - strand at 4365.369 kb on - strand at 4365.369 kb on - strand at 4365.450 kb on + strand at 4365.450 kb on + strand at 4365.451 kb on - strand at 4365.451 kb on - strand at 4365.451 kb on - strand at 4365.469 kb on + strand at 4365.488 kb on + strand at 4365.489 kb on - strand at 4365.489 kb on - strand at 4365.489 kb on - strand at 4365.489 kb on - strand at 4365.489 kb on - strand at 4365.512 kb on - strand at 4365.542 kb on + strand at 4365.546 kb on + strand at 4365.626 kb on + strand at 4365.627 kb on - strand at 4366.851 kb on - strand at 4366.855 kb on - strand at 4366.855 kb on - strand at 4366.855 kb on - strand at 4366.878 kb on - strand at 4366.892 kb on - strand at 4366.892 kb on - strand at 4366.892 kb on - strand at 4366.892 kb on - strand at 4367.127 kb on - strand at 4367.378 kb on + strand, within Pf6N2E2_3751 at 4367.378 kb on + strand, within Pf6N2E2_3751 at 4367.379 kb on - strand, within Pf6N2E2_3751 at 4367.379 kb on - strand, within Pf6N2E2_3751 at 4367.456 kb on + strand, within Pf6N2E2_3751 at 4367.634 kb on - strand, within Pf6N2E2_3751 at 4367.675 kb on + strand, within Pf6N2E2_3751 at 4367.675 kb on + strand, within Pf6N2E2_3751 at 4367.675 kb on + strand, within Pf6N2E2_3751 at 4367.675 kb on + strand, within Pf6N2E2_3751 at 4367.675 kb on + strand, within Pf6N2E2_3751 at 4367.675 kb on + strand, within Pf6N2E2_3751 at 4367.675 kb on + strand, within Pf6N2E2_3751 at 4367.675 kb on + strand, within Pf6N2E2_3751 at 4367.675 kb on + strand, within Pf6N2E2_3751 at 4367.676 kb on - strand, within Pf6N2E2_3751 at 4367.676 kb on - strand, within Pf6N2E2_3751 at 4367.676 kb on - strand, within Pf6N2E2_3751 at 4367.676 kb on - strand, within Pf6N2E2_3751 at 4367.676 kb on - strand, within Pf6N2E2_3751 at 4367.676 kb on - strand, within Pf6N2E2_3751 at 4367.676 kb on - strand, within Pf6N2E2_3751 at 4367.676 kb on - strand, within Pf6N2E2_3751 at 4367.676 kb on - strand, within Pf6N2E2_3751 at 4367.681 kb on + strand, within Pf6N2E2_3751 at 4367.681 kb on + strand, within Pf6N2E2_3751 at 4367.681 kb on + strand, within Pf6N2E2_3751 at 4367.681 kb on + strand, within Pf6N2E2_3751 at 4367.681 kb on + strand, within Pf6N2E2_3751 at 4367.681 kb on + strand, within Pf6N2E2_3751 at 4367.681 kb on + strand, within Pf6N2E2_3751 at 4367.682 kb on - strand, within Pf6N2E2_3751 at 4367.682 kb on - strand, within Pf6N2E2_3751 at 4367.682 kb on - strand, within Pf6N2E2_3751 at 4367.682 kb on - strand, within Pf6N2E2_3751 at 4367.711 kb on + strand, within Pf6N2E2_3751 at 4367.711 kb on + strand, within Pf6N2E2_3751 at 4367.711 kb on + strand, within Pf6N2E2_3751 at 4367.712 kb on - strand, within Pf6N2E2_3751
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 4,364,765 + Pf6N2E2_3749 0.59 +0.7 4,364,765 + Pf6N2E2_3749 0.59 +0.5 4,364,766 - Pf6N2E2_3749 0.59 -0.5 4,364,826 + Pf6N2E2_3749 0.63 -0.5 4,364,826 + Pf6N2E2_3749 0.63 +0.4 4,364,827 - Pf6N2E2_3749 0.63 -0.2 4,364,827 - Pf6N2E2_3749 0.63 +1.8 4,364,892 + Pf6N2E2_3749 0.68 +0.5 4,364,966 + Pf6N2E2_3749 0.73 +0.6 4,364,967 - Pf6N2E2_3749 0.73 +0.2 4,364,967 - Pf6N2E2_3749 0.73 -0.3 4,364,967 - Pf6N2E2_3749 0.73 -0.2 4,364,967 - Pf6N2E2_3749 0.73 +0.1 4,364,999 + Pf6N2E2_3749 0.75 +0.6 4,364,999 + Pf6N2E2_3749 0.75 -0.1 4,364,999 + Pf6N2E2_3749 0.75 +0.7 4,365,000 - Pf6N2E2_3749 0.75 -1.0 4,365,000 - Pf6N2E2_3749 0.75 +0.2 4,365,180 + Pf6N2E2_3749 0.87 -0.3 4,365,180 + Pf6N2E2_3749 0.87 -0.7 4,365,180 + Pf6N2E2_3749 0.87 -0.4 4,365,180 + Pf6N2E2_3749 0.87 -1.4 4,365,181 - Pf6N2E2_3749 0.87 -0.9 4,365,191 + Pf6N2E2_3749 0.88 -2.2 4,365,211 + Pf6N2E2_3749 0.89 -1.8 4,365,337 + +0.3 4,365,337 + +0.7 4,365,337 + +1.3 4,365,337 + +0.3 4,365,338 - +0.3 4,365,338 - +0.1 4,365,338 - +0.2 4,365,338 - -0.3 4,365,369 - -1.9 4,365,369 - +0.9 4,365,450 + +0.3 4,365,450 + -0.6 4,365,451 - +0.3 4,365,451 - +1.3 4,365,451 - -0.8 4,365,469 + -0.3 4,365,488 + +0.6 4,365,489 - -1.0 4,365,489 - +0.6 4,365,489 - -0.2 4,365,489 - +0.3 4,365,489 - -1.7 4,365,512 - -0.3 4,365,542 + -0.5 4,365,546 + +1.3 4,365,626 + +0.7 4,365,627 - -0.8 4,366,851 - +0.0 4,366,855 - +0.7 4,366,855 - -1.8 4,366,855 - +0.6 4,366,878 - +1.3 4,366,892 - -0.9 4,366,892 - -0.1 4,366,892 - +0.1 4,366,892 - -0.1 4,367,127 - -4.4 4,367,378 + Pf6N2E2_3751 0.27 -4.1 4,367,378 + Pf6N2E2_3751 0.27 -2.8 4,367,379 - Pf6N2E2_3751 0.27 -2.3 4,367,379 - Pf6N2E2_3751 0.27 -3.0 4,367,456 + Pf6N2E2_3751 0.32 -2.6 4,367,634 - Pf6N2E2_3751 0.45 -3.7 4,367,675 + Pf6N2E2_3751 0.48 -2.2 4,367,675 + Pf6N2E2_3751 0.48 -1.9 4,367,675 + Pf6N2E2_3751 0.48 -1.9 4,367,675 + Pf6N2E2_3751 0.48 -1.5 4,367,675 + Pf6N2E2_3751 0.48 -3.7 4,367,675 + Pf6N2E2_3751 0.48 -3.6 4,367,675 + Pf6N2E2_3751 0.48 -2.3 4,367,675 + Pf6N2E2_3751 0.48 -2.0 4,367,675 + Pf6N2E2_3751 0.48 -3.4 4,367,676 - Pf6N2E2_3751 0.48 -3.7 4,367,676 - Pf6N2E2_3751 0.48 -0.3 4,367,676 - Pf6N2E2_3751 0.48 -2.6 4,367,676 - Pf6N2E2_3751 0.48 -3.3 4,367,676 - Pf6N2E2_3751 0.48 -3.9 4,367,676 - Pf6N2E2_3751 0.48 -2.0 4,367,676 - Pf6N2E2_3751 0.48 -5.1 4,367,676 - Pf6N2E2_3751 0.48 -3.1 4,367,676 - Pf6N2E2_3751 0.48 -5.0 4,367,681 + Pf6N2E2_3751 0.48 -3.6 4,367,681 + Pf6N2E2_3751 0.48 -1.9 4,367,681 + Pf6N2E2_3751 0.48 -2.6 4,367,681 + Pf6N2E2_3751 0.48 -2.7 4,367,681 + Pf6N2E2_3751 0.48 -3.4 4,367,681 + Pf6N2E2_3751 0.48 -0.7 4,367,681 + Pf6N2E2_3751 0.48 -2.9 4,367,682 - Pf6N2E2_3751 0.48 -2.4 4,367,682 - Pf6N2E2_3751 0.48 -0.7 4,367,682 - Pf6N2E2_3751 0.48 -2.6 4,367,682 - Pf6N2E2_3751 0.48 -4.1 4,367,711 + Pf6N2E2_3751 0.50 -0.3 4,367,711 + Pf6N2E2_3751 0.50 -2.2 4,367,711 + Pf6N2E2_3751 0.50 -3.8 4,367,712 - Pf6N2E2_3751 0.50 -4.7
Or see this region's nucleotide sequence