Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3657 and Pf6N2E2_3658 are separated by 150 nucleotides Pf6N2E2_3658 and Pf6N2E2_3659 are separated by 45 nucleotides
Pf6N2E2_3657 - Lipid A export ATP-binding/permease protein MsbA, at 4,258,010 to 4,259,800
_3657
Pf6N2E2_3658 - Lipid A core - O-antigen ligase and related enzymes, at 4,259,951 to 4,261,084
_3658
Pf6N2E2_3659 - Protein fixF, at 4,261,130 to 4,262,236
_3659
Position (kb)
4259
4260
4261
4262 Strain fitness (log2 ratio)
-2
-1
0
1 at 4259.846 kb on - strand at 4259.861 kb on - strand at 4259.953 kb on - strand at 4259.999 kb on - strand at 4259.999 kb on - strand at 4259.999 kb on - strand at 4260.255 kb on - strand, within Pf6N2E2_3658 at 4260.255 kb on - strand, within Pf6N2E2_3658 at 4260.277 kb on - strand, within Pf6N2E2_3658 at 4260.326 kb on - strand, within Pf6N2E2_3658 at 4260.339 kb on - strand, within Pf6N2E2_3658 at 4260.339 kb on - strand, within Pf6N2E2_3658 at 4260.372 kb on - strand, within Pf6N2E2_3658 at 4260.390 kb on - strand, within Pf6N2E2_3658 at 4260.760 kb on - strand, within Pf6N2E2_3658 at 4260.831 kb on - strand, within Pf6N2E2_3658 at 4260.831 kb on - strand, within Pf6N2E2_3658 at 4260.831 kb on - strand, within Pf6N2E2_3658 at 4260.834 kb on + strand, within Pf6N2E2_3658 at 4260.834 kb on + strand, within Pf6N2E2_3658 at 4260.834 kb on + strand, within Pf6N2E2_3658 at 4260.834 kb on + strand, within Pf6N2E2_3658 at 4260.834 kb on + strand, within Pf6N2E2_3658 at 4260.834 kb on + strand, within Pf6N2E2_3658 at 4260.834 kb on + strand, within Pf6N2E2_3658 at 4260.834 kb on + strand at 4260.834 kb on + strand, within Pf6N2E2_3658 at 4260.835 kb on - strand, within Pf6N2E2_3658 at 4260.835 kb on - strand, within Pf6N2E2_3658 at 4260.835 kb on - strand, within Pf6N2E2_3658 at 4260.835 kb on - strand, within Pf6N2E2_3658 at 4260.835 kb on - strand, within Pf6N2E2_3658 at 4260.835 kb on - strand, within Pf6N2E2_3658 at 4260.835 kb on - strand, within Pf6N2E2_3658 at 4260.835 kb on - strand, within Pf6N2E2_3658 at 4260.945 kb on - strand, within Pf6N2E2_3658 at 4260.998 kb on + strand at 4260.998 kb on + strand at 4260.998 kb on + strand at 4260.999 kb on - strand at 4260.999 kb on - strand at 4261.052 kb on + strand at 4261.053 kb on - strand at 4261.053 kb on - strand at 4261.105 kb on + strand at 4261.131 kb on + strand at 4261.177 kb on + strand at 4261.178 kb on - strand at 4261.202 kb on - strand at 4261.210 kb on - strand at 4261.220 kb on - strand at 4261.224 kb on + strand at 4261.225 kb on - strand at 4261.238 kb on - strand at 4261.238 kb on - strand at 4261.238 kb on - strand at 4261.238 kb on - strand at 4261.244 kb on + strand, within Pf6N2E2_3659 at 4261.245 kb on - strand, within Pf6N2E2_3659 at 4261.331 kb on + strand, within Pf6N2E2_3659 at 4261.331 kb on + strand, within Pf6N2E2_3659 at 4261.332 kb on - strand, within Pf6N2E2_3659 at 4261.332 kb on - strand, within Pf6N2E2_3659 at 4261.332 kb on - strand, within Pf6N2E2_3659 at 4261.332 kb on - strand, within Pf6N2E2_3659 at 4261.358 kb on - strand, within Pf6N2E2_3659 at 4261.358 kb on - strand, within Pf6N2E2_3659 at 4261.454 kb on - strand, within Pf6N2E2_3659 at 4261.752 kb on + strand, within Pf6N2E2_3659 at 4261.759 kb on - strand, within Pf6N2E2_3659 at 4261.759 kb on - strand, within Pf6N2E2_3659 at 4261.759 kb on - strand, within Pf6N2E2_3659 at 4261.759 kb on - strand, within Pf6N2E2_3659 at 4261.759 kb on - strand, within Pf6N2E2_3659 at 4261.759 kb on - strand, within Pf6N2E2_3659 at 4261.800 kb on + strand, within Pf6N2E2_3659 at 4262.000 kb on + strand, within Pf6N2E2_3659
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 4,259,846 - -1.7 4,259,861 - -1.6 4,259,953 - +0.3 4,259,999 - -1.2 4,259,999 - -1.9 4,259,999 - +0.3 4,260,255 - Pf6N2E2_3658 0.27 +1.3 4,260,255 - Pf6N2E2_3658 0.27 +1.3 4,260,277 - Pf6N2E2_3658 0.29 +1.3 4,260,326 - Pf6N2E2_3658 0.33 +0.3 4,260,339 - Pf6N2E2_3658 0.34 -2.5 4,260,339 - Pf6N2E2_3658 0.34 +1.3 4,260,372 - Pf6N2E2_3658 0.37 +1.3 4,260,390 - Pf6N2E2_3658 0.39 +1.3 4,260,760 - Pf6N2E2_3658 0.71 +1.3 4,260,831 - Pf6N2E2_3658 0.78 +1.3 4,260,831 - Pf6N2E2_3658 0.78 -1.3 4,260,831 - Pf6N2E2_3658 0.78 +1.3 4,260,834 + Pf6N2E2_3658 0.78 -2.5 4,260,834 + Pf6N2E2_3658 0.78 -0.6 4,260,834 + Pf6N2E2_3658 0.78 -1.2 4,260,834 + Pf6N2E2_3658 0.78 -1.8 4,260,834 + Pf6N2E2_3658 0.78 -2.4 4,260,834 + Pf6N2E2_3658 0.78 -2.7 4,260,834 + Pf6N2E2_3658 0.78 -2.0 4,260,834 + +0.3 4,260,834 + Pf6N2E2_3658 0.78 +0.0 4,260,835 - Pf6N2E2_3658 0.78 +1.3 4,260,835 - Pf6N2E2_3658 0.78 +1.3 4,260,835 - Pf6N2E2_3658 0.78 -0.3 4,260,835 - Pf6N2E2_3658 0.78 +1.3 4,260,835 - Pf6N2E2_3658 0.78 +1.3 4,260,835 - Pf6N2E2_3658 0.78 -0.7 4,260,835 - Pf6N2E2_3658 0.78 +1.3 4,260,835 - Pf6N2E2_3658 0.78 -1.6 4,260,945 - Pf6N2E2_3658 0.88 +0.3 4,260,998 + -0.0 4,260,998 + -0.0 4,260,998 + -2.3 4,260,999 - +1.3 4,260,999 - +1.3 4,261,052 + +0.6 4,261,053 - +1.3 4,261,053 - +0.3 4,261,105 + -1.7 4,261,131 + -1.9 4,261,177 + +0.3 4,261,178 - +1.3 4,261,202 - +1.3 4,261,210 - +1.3 4,261,220 - -1.0 4,261,224 + -2.7 4,261,225 - -0.8 4,261,238 - +0.1 4,261,238 - +0.3 4,261,238 - +1.3 4,261,238 - +1.3 4,261,244 + Pf6N2E2_3659 0.10 -1.7 4,261,245 - Pf6N2E2_3659 0.10 +0.3 4,261,331 + Pf6N2E2_3659 0.18 +0.1 4,261,331 + Pf6N2E2_3659 0.18 -0.5 4,261,332 - Pf6N2E2_3659 0.18 +1.3 4,261,332 - Pf6N2E2_3659 0.18 +1.3 4,261,332 - Pf6N2E2_3659 0.18 +1.3 4,261,332 - Pf6N2E2_3659 0.18 +1.3 4,261,358 - Pf6N2E2_3659 0.21 -1.0 4,261,358 - Pf6N2E2_3659 0.21 +0.3 4,261,454 - Pf6N2E2_3659 0.29 +1.3 4,261,752 + Pf6N2E2_3659 0.56 -0.9 4,261,759 - Pf6N2E2_3659 0.57 -1.0 4,261,759 - Pf6N2E2_3659 0.57 +1.3 4,261,759 - Pf6N2E2_3659 0.57 +1.3 4,261,759 - Pf6N2E2_3659 0.57 -0.3 4,261,759 - Pf6N2E2_3659 0.57 +0.3 4,261,759 - Pf6N2E2_3659 0.57 -0.3 4,261,800 + Pf6N2E2_3659 0.61 -0.3 4,262,000 + Pf6N2E2_3659 0.79 -1.5
Or see this region's nucleotide sequence