Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_348 and Pf6N2E2_349 overlap by 4 nucleotides Pf6N2E2_349 and Pf6N2E2_350 are separated by 65 nucleotides Pf6N2E2_350 and Pf6N2E2_351 are separated by 5 nucleotides
Pf6N2E2_348 - Transcriptional regulator, LysR family, at 414,114 to 415,043
_348
Pf6N2E2_349 - hypothetical protein, at 415,040 to 415,774
_349
Pf6N2E2_350 - Gfa-like protein, at 415,840 to 416,232
_350
Pf6N2E2_351 - Aldehyde dehydrogenase (EC 1.2.1.3), at 416,238 to 417,686
_351
Position (kb)
415
416
417 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 414.849 kb on + strand, within Pf6N2E2_348 at 414.849 kb on + strand, within Pf6N2E2_348 at 414.849 kb on + strand, within Pf6N2E2_348 at 414.849 kb on + strand, within Pf6N2E2_348 at 414.849 kb on + strand, within Pf6N2E2_348 at 414.850 kb on - strand, within Pf6N2E2_348 at 414.850 kb on - strand, within Pf6N2E2_348 at 414.850 kb on - strand, within Pf6N2E2_348 at 414.850 kb on - strand, within Pf6N2E2_348 at 414.927 kb on + strand, within Pf6N2E2_348 at 414.927 kb on + strand, within Pf6N2E2_348 at 415.062 kb on + strand at 415.062 kb on + strand at 415.063 kb on - strand at 415.063 kb on - strand at 415.073 kb on + strand at 415.073 kb on + strand at 415.074 kb on - strand at 415.074 kb on - strand at 415.074 kb on - strand at 415.082 kb on + strand at 415.083 kb on - strand at 415.083 kb on - strand at 415.347 kb on + strand, within Pf6N2E2_349 at 415.515 kb on + strand, within Pf6N2E2_349 at 415.516 kb on - strand, within Pf6N2E2_349 at 415.542 kb on - strand, within Pf6N2E2_349 at 415.660 kb on + strand, within Pf6N2E2_349 at 415.660 kb on + strand, within Pf6N2E2_349 at 415.772 kb on + strand at 415.773 kb on - strand at 415.806 kb on + strand at 415.807 kb on - strand at 415.807 kb on - strand at 415.807 kb on - strand at 415.807 kb on - strand at 415.865 kb on + strand at 416.131 kb on + strand, within Pf6N2E2_350 at 416.192 kb on - strand, within Pf6N2E2_350 at 416.231 kb on - strand at 416.231 kb on - strand at 416.272 kb on + strand at 416.272 kb on + strand at 416.272 kb on + strand at 416.297 kb on - strand at 416.323 kb on + strand at 416.449 kb on + strand, within Pf6N2E2_351 at 416.584 kb on + strand, within Pf6N2E2_351 at 416.584 kb on + strand, within Pf6N2E2_351 at 416.585 kb on - strand, within Pf6N2E2_351 at 417.212 kb on - strand, within Pf6N2E2_351
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 414,849 + Pf6N2E2_348 0.79 +1.8 414,849 + Pf6N2E2_348 0.79 +0.4 414,849 + Pf6N2E2_348 0.79 -1.4 414,849 + Pf6N2E2_348 0.79 +0.3 414,849 + Pf6N2E2_348 0.79 +0.1 414,850 - Pf6N2E2_348 0.79 -2.1 414,850 - Pf6N2E2_348 0.79 +0.2 414,850 - Pf6N2E2_348 0.79 +2.6 414,850 - Pf6N2E2_348 0.79 +0.4 414,927 + Pf6N2E2_348 0.87 +0.6 414,927 + Pf6N2E2_348 0.87 +0.2 415,062 + -0.8 415,062 + +0.2 415,063 - +0.4 415,063 - -0.0 415,073 + +1.0 415,073 + +1.2 415,074 - +0.6 415,074 - -0.1 415,074 - -0.1 415,082 + +0.1 415,083 - +0.5 415,083 - +1.4 415,347 + Pf6N2E2_349 0.42 +0.5 415,515 + Pf6N2E2_349 0.65 -0.3 415,516 - Pf6N2E2_349 0.65 +1.0 415,542 - Pf6N2E2_349 0.68 +1.4 415,660 + Pf6N2E2_349 0.84 -0.2 415,660 + Pf6N2E2_349 0.84 -0.1 415,772 + +0.4 415,773 - -0.6 415,806 + -2.3 415,807 - +0.6 415,807 - +0.4 415,807 - -0.5 415,807 - -1.6 415,865 + +0.3 416,131 + Pf6N2E2_350 0.74 -1.6 416,192 - Pf6N2E2_350 0.90 -1.1 416,231 - -0.4 416,231 - +0.2 416,272 + +0.8 416,272 + -0.1 416,272 + +0.2 416,297 - -1.9 416,323 + -0.5 416,449 + Pf6N2E2_351 0.15 +0.4 416,584 + Pf6N2E2_351 0.24 -0.6 416,584 + Pf6N2E2_351 0.24 -0.1 416,585 - Pf6N2E2_351 0.24 +0.2 417,212 - Pf6N2E2_351 0.67 +0.0
Or see this region's nucleotide sequence