Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_336 and Pf6N2E2_337 are separated by 173 nucleotides Pf6N2E2_337 and Pf6N2E2_338 are separated by 147 nucleotides Pf6N2E2_338 and Pf6N2E2_339 are separated by 6 nucleotides
Pf6N2E2_336 - hypothetical protein, at 403,631 to 404,413
_336
Pf6N2E2_337 - hypothetical protein, at 404,587 to 404,859
_337
Pf6N2E2_338 - Transcriptional regulator, AraC family, at 405,007 to 405,510
_338
Pf6N2E2_339 - Glutathione-regulated potassium-efflux system protein KefC, at 405,517 to 407,343
_339
Position (kb) 405
406 Strain fitness (log2 ratio) -1
0
1
2 at 404.039 kb on - strand, within Pf6N2E2_336 at 404.039 kb on - strand, within Pf6N2E2_336 at 404.039 kb on - strand, within Pf6N2E2_336 at 404.116 kb on - strand, within Pf6N2E2_336 at 404.467 kb on + strand at 404.467 kb on + strand at 404.536 kb on + strand at 404.536 kb on + strand at 404.536 kb on + strand at 404.537 kb on - strand at 404.669 kb on - strand, within Pf6N2E2_337 at 404.875 kb on + strand at 404.875 kb on + strand at 404.876 kb on - strand at 405.098 kb on + strand, within Pf6N2E2_338 at 405.098 kb on + strand, within Pf6N2E2_338 at 405.732 kb on - strand, within Pf6N2E2_339 at 405.732 kb on - strand, within Pf6N2E2_339 at 406.027 kb on - strand, within Pf6N2E2_339 at 406.064 kb on - strand, within Pf6N2E2_339
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 404,039 - Pf6N2E2_336 0.52 -0.5 404,039 - Pf6N2E2_336 0.52 -0.5 404,039 - Pf6N2E2_336 0.52 -0.1 404,116 - Pf6N2E2_336 0.62 +2.4 404,467 + +0.4 404,467 + +1.4 404,536 + +0.9 404,536 + +1.2 404,536 + +0.8 404,537 - +1.4 404,669 - Pf6N2E2_337 0.30 +1.4 404,875 + -0.1 404,875 + -0.2 404,876 - -0.8 405,098 + Pf6N2E2_338 0.18 +1.1 405,098 + Pf6N2E2_338 0.18 +1.5 405,732 - Pf6N2E2_339 0.12 +1.4 405,732 - Pf6N2E2_339 0.12 -0.1 406,027 - Pf6N2E2_339 0.28 +0.7 406,064 - Pf6N2E2_339 0.30 +1.4
Or see this region's nucleotide sequence