Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3255

Experiment: Nitrate reduction, replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3254 and Pf6N2E2_3255 are separated by 22 nucleotidesPf6N2E2_3255 and Pf6N2E2_3256 are separated by 223 nucleotidesPf6N2E2_3256 and Pf6N2E2_3257 overlap by 4 nucleotides Pf6N2E2_3254 - UDP-N-acetylglucosamine 1-carboxyvinyltransferase (EC 2.5.1.7), at 3,764,696 to 3,765,961 _3254 Pf6N2E2_3255 - YrbA protein, at 3,765,984 to 3,766,223 _3255 Pf6N2E2_3256 - Uncharacterized protein YrbB, at 3,766,447 to 3,766,764 _3256 Pf6N2E2_3257 - Uncharacterized ABC transporter, auxiliary component YrbC, at 3,766,761 to 3,767,414 _3257 Position (kb) 3765 3766 3767Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3766.076 kb on - strand, within Pf6N2E2_3255at 3766.076 kb on - strand, within Pf6N2E2_3255at 3766.076 kb on - strand, within Pf6N2E2_3255at 3766.076 kb on - strand, within Pf6N2E2_3255at 3766.076 kb on - strand, within Pf6N2E2_3255at 3766.076 kb on - strand, within Pf6N2E2_3255at 3766.195 kb on - strand, within Pf6N2E2_3255at 3766.195 kb on - strand, within Pf6N2E2_3255at 3766.195 kb on - strand, within Pf6N2E2_3255at 3766.213 kb on - strandat 3766.317 kb on + strandat 3766.489 kb on + strand, within Pf6N2E2_3256at 3766.966 kb on + strand, within Pf6N2E2_3257at 3766.978 kb on + strand, within Pf6N2E2_3257at 3766.979 kb on - strand, within Pf6N2E2_3257at 3767.074 kb on + strand, within Pf6N2E2_3257at 3767.074 kb on + strand, within Pf6N2E2_3257at 3767.075 kb on - strand, within Pf6N2E2_3257at 3767.167 kb on + strand, within Pf6N2E2_3257at 3767.167 kb on + strand, within Pf6N2E2_3257at 3767.167 kb on + strand, within Pf6N2E2_3257at 3767.168 kb on - strand, within Pf6N2E2_3257

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A
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3,766,076 - Pf6N2E2_3255 0.38 +1.4
3,766,076 - Pf6N2E2_3255 0.38 -2.3
3,766,076 - Pf6N2E2_3255 0.38 +1.4
3,766,076 - Pf6N2E2_3255 0.38 -0.9
3,766,076 - Pf6N2E2_3255 0.38 -1.6
3,766,076 - Pf6N2E2_3255 0.38 -0.6
3,766,195 - Pf6N2E2_3255 0.88 -3.4
3,766,195 - Pf6N2E2_3255 0.88 -3.0
3,766,195 - Pf6N2E2_3255 0.88 -2.1
3,766,213 - -2.4
3,766,317 + +0.3
3,766,489 + Pf6N2E2_3256 0.13 +1.4
3,766,966 + Pf6N2E2_3257 0.31 -0.2
3,766,978 + Pf6N2E2_3257 0.33 -0.6
3,766,979 - Pf6N2E2_3257 0.33 -0.8
3,767,074 + Pf6N2E2_3257 0.48 +1.4
3,767,074 + Pf6N2E2_3257 0.48 -1.4
3,767,075 - Pf6N2E2_3257 0.48 +2.0
3,767,167 + Pf6N2E2_3257 0.62 +1.4
3,767,167 + Pf6N2E2_3257 0.62 -0.2
3,767,167 + Pf6N2E2_3257 0.62 -2.4
3,767,168 - Pf6N2E2_3257 0.62 +1.4

Or see this region's nucleotide sequence