Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_281 and Pf6N2E2_282 are separated by 170 nucleotides Pf6N2E2_282 and Pf6N2E2_283 overlap by 8 nucleotides
Pf6N2E2_281 - Type II and III secretion system family protein, at 347,655 to 348,416
_281
Pf6N2E2_282 - GNAT family acetyltransferase YjcF, at 348,587 to 349,012
_282
Pf6N2E2_283 - FIG002776: hypothetical protein, at 349,005 to 350,171
_283
Position (kb) 348
349
350 Strain fitness (log2 ratio) -1
0
1 at 347.611 kb on + strand at 347.713 kb on + strand at 347.713 kb on + strand at 347.713 kb on + strand at 347.714 kb on - strand at 347.826 kb on + strand, within Pf6N2E2_281 at 347.827 kb on - strand, within Pf6N2E2_281 at 347.827 kb on - strand, within Pf6N2E2_281 at 347.827 kb on - strand at 347.911 kb on + strand, within Pf6N2E2_281 at 347.912 kb on - strand, within Pf6N2E2_281 at 347.912 kb on - strand, within Pf6N2E2_281 at 347.962 kb on + strand, within Pf6N2E2_281 at 347.962 kb on + strand, within Pf6N2E2_281 at 347.962 kb on + strand, within Pf6N2E2_281 at 347.962 kb on + strand, within Pf6N2E2_281 at 347.963 kb on - strand, within Pf6N2E2_281 at 347.963 kb on - strand, within Pf6N2E2_281 at 347.963 kb on - strand, within Pf6N2E2_281 at 348.165 kb on - strand, within Pf6N2E2_281 at 348.446 kb on + strand at 348.487 kb on + strand at 348.487 kb on + strand at 348.487 kb on + strand at 348.488 kb on - strand at 348.488 kb on - strand at 348.488 kb on - strand at 348.672 kb on + strand, within Pf6N2E2_282 at 348.672 kb on + strand, within Pf6N2E2_282 at 349.006 kb on + strand at 349.007 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 347,611 + -1.0 347,713 + -0.3 347,713 + +1.2 347,713 + -0.8 347,714 - +0.3 347,826 + Pf6N2E2_281 0.22 +0.4 347,827 - Pf6N2E2_281 0.23 +0.2 347,827 - Pf6N2E2_281 0.23 +0.2 347,827 - +1.4 347,911 + Pf6N2E2_281 0.34 +0.6 347,912 - Pf6N2E2_281 0.34 +0.1 347,912 - Pf6N2E2_281 0.34 +0.2 347,962 + Pf6N2E2_281 0.40 -0.7 347,962 + Pf6N2E2_281 0.40 -0.7 347,962 + Pf6N2E2_281 0.40 +0.5 347,962 + Pf6N2E2_281 0.40 +0.4 347,963 - Pf6N2E2_281 0.40 +1.4 347,963 - Pf6N2E2_281 0.40 -0.4 347,963 - Pf6N2E2_281 0.40 -0.2 348,165 - Pf6N2E2_281 0.67 +0.0 348,446 + +0.1 348,487 + +0.4 348,487 + +1.0 348,487 + -0.5 348,488 - +0.8 348,488 - +1.4 348,488 - -0.7 348,672 + Pf6N2E2_282 0.20 +1.2 348,672 + Pf6N2E2_282 0.20 +0.5 349,006 + +0.9 349,007 - -0.1
Or see this region's nucleotide sequence