Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_28

Experiment: Nitrate reduction, replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_27 and Pf6N2E2_28 are separated by 306 nucleotidesPf6N2E2_28 and Pf6N2E2_29 are separated by 223 nucleotidesPf6N2E2_29 and Pf6N2E2_30 are separated by 37 nucleotides Pf6N2E2_27 - Mechanosensitive ion channel, at 63,992 to 65,434 _27 Pf6N2E2_28 - 4-carboxymuconolactone decarboxylase domain/alkylhydroperoxidase AhpD family core domain protein, at 65,741 to 66,181 _28 Pf6N2E2_29 - Sigma-70 factor FpvI (ECF subfamily), controling pyoverdin biosynthesis @ FIG006045: Sigma factor, ECF subfamily, at 66,405 to 66,905 _29 Pf6N2E2_30 - Antisigma transmembrane sensor FpvR, at 66,943 to 67,926 _30 Position (kb) 65 66 67Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 64.814 kb on + strand, within Pf6N2E2_27at 64.815 kb on - strand, within Pf6N2E2_27at 65.279 kb on + strand, within Pf6N2E2_27at 65.280 kb on - strand, within Pf6N2E2_27at 65.343 kb on + strandat 65.344 kb on - strandat 65.344 kb on - strandat 65.344 kb on - strandat 65.344 kb on - strandat 65.344 kb on - strandat 65.344 kb on - strandat 65.344 kb on - strandat 65.686 kb on - strandat 65.709 kb on + strandat 65.709 kb on + strandat 65.715 kb on + strandat 65.716 kb on - strandat 65.762 kb on + strandat 65.763 kb on - strandat 66.062 kb on + strand, within Pf6N2E2_28at 66.063 kb on - strand, within Pf6N2E2_28at 66.063 kb on - strand, within Pf6N2E2_28at 66.208 kb on + strandat 66.209 kb on - strandat 66.248 kb on + strandat 66.248 kb on + strandat 66.248 kb on + strandat 66.248 kb on + strandat 66.249 kb on - strandat 66.249 kb on - strandat 66.296 kb on + strandat 66.296 kb on + strandat 66.296 kb on + strandat 66.297 kb on - strandat 66.297 kb on - strandat 66.297 kb on - strandat 66.302 kb on + strandat 66.302 kb on + strandat 66.303 kb on - strandat 66.303 kb on - strandat 66.303 kb on - strandat 66.303 kb on - strandat 66.303 kb on - strandat 66.303 kb on - strandat 66.303 kb on - strandat 66.342 kb on + strandat 66.343 kb on - strandat 66.343 kb on - strandat 66.343 kb on - strandat 66.355 kb on + strandat 66.447 kb on + strandat 66.448 kb on - strandat 66.448 kb on - strandat 66.574 kb on - strand, within Pf6N2E2_29at 66.577 kb on + strand, within Pf6N2E2_29at 66.577 kb on + strand, within Pf6N2E2_29at 66.577 kb on + strand, within Pf6N2E2_29at 66.577 kb on + strand, within Pf6N2E2_29at 66.577 kb on + strand, within Pf6N2E2_29at 66.577 kb on + strand, within Pf6N2E2_29at 66.662 kb on + strand, within Pf6N2E2_29at 66.663 kb on - strand, within Pf6N2E2_29at 66.693 kb on + strand, within Pf6N2E2_29at 66.693 kb on + strand, within Pf6N2E2_29at 66.693 kb on + strand, within Pf6N2E2_29at 66.693 kb on + strand, within Pf6N2E2_29at 66.694 kb on - strand, within Pf6N2E2_29at 66.694 kb on - strand, within Pf6N2E2_29at 66.694 kb on - strand, within Pf6N2E2_29at 66.694 kb on - strand, within Pf6N2E2_29at 66.694 kb on - strand, within Pf6N2E2_29at 66.849 kb on + strand, within Pf6N2E2_29at 66.850 kb on - strand, within Pf6N2E2_29at 66.850 kb on - strand, within Pf6N2E2_29at 66.851 kb on + strand, within Pf6N2E2_29at 66.851 kb on + strand, within Pf6N2E2_29at 66.852 kb on - strand, within Pf6N2E2_29at 66.852 kb on - strand, within Pf6N2E2_29at 67.113 kb on + strand, within Pf6N2E2_30at 67.113 kb on + strand, within Pf6N2E2_30

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A
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64,814 + Pf6N2E2_27 0.57 +1.4
64,815 - Pf6N2E2_27 0.57 +0.8
65,279 + Pf6N2E2_27 0.89 +0.7
65,280 - Pf6N2E2_27 0.89 +0.2
65,343 + +0.2
65,344 - -0.6
65,344 - +0.8
65,344 - +0.5
65,344 - -1.3
65,344 - +0.0
65,344 - +1.0
65,344 - +1.4
65,686 - -0.1
65,709 + -0.4
65,709 + -0.5
65,715 + +2.0
65,716 - -3.2
65,762 + +1.4
65,763 - +0.6
66,062 + Pf6N2E2_28 0.73 -0.8
66,063 - Pf6N2E2_28 0.73 -1.1
66,063 - Pf6N2E2_28 0.73 -0.9
66,208 + -0.8
66,209 - -0.2
66,248 + -0.4
66,248 + -0.9
66,248 + +0.6
66,248 + +1.4
66,249 - -0.6
66,249 - -0.9
66,296 + -0.4
66,296 + -0.6
66,296 + +0.1
66,297 - -0.1
66,297 - -0.1
66,297 - -1.0
66,302 + +1.1
66,302 + +0.9
66,303 - -1.1
66,303 - +0.2
66,303 - -0.3
66,303 - +0.7
66,303 - +1.4
66,303 - +0.7
66,303 - -0.5
66,342 + -0.8
66,343 - -0.3
66,343 - +0.8
66,343 - -0.4
66,355 + +0.9
66,447 + +0.3
66,448 - +0.5
66,448 - +1.0
66,574 - Pf6N2E2_29 0.34 -0.0
66,577 + Pf6N2E2_29 0.34 -1.0
66,577 + Pf6N2E2_29 0.34 +0.6
66,577 + Pf6N2E2_29 0.34 +1.4
66,577 + Pf6N2E2_29 0.34 -0.6
66,577 + Pf6N2E2_29 0.34 +0.1
66,577 + Pf6N2E2_29 0.34 +0.2
66,662 + Pf6N2E2_29 0.51 +0.6
66,663 - Pf6N2E2_29 0.51 +0.3
66,693 + Pf6N2E2_29 0.57 -0.6
66,693 + Pf6N2E2_29 0.57 +3.9
66,693 + Pf6N2E2_29 0.57 -2.3
66,693 + Pf6N2E2_29 0.57 +1.0
66,694 - Pf6N2E2_29 0.58 +0.1
66,694 - Pf6N2E2_29 0.58 +0.1
66,694 - Pf6N2E2_29 0.58 +0.5
66,694 - Pf6N2E2_29 0.58 +0.2
66,694 - Pf6N2E2_29 0.58 -0.3
66,849 + Pf6N2E2_29 0.89 -1.8
66,850 - Pf6N2E2_29 0.89 -0.2
66,850 - Pf6N2E2_29 0.89 +1.4
66,851 + Pf6N2E2_29 0.89 -0.3
66,851 + Pf6N2E2_29 0.89 +0.1
66,852 - Pf6N2E2_29 0.89 -0.8
66,852 - Pf6N2E2_29 0.89 +0.1
67,113 + Pf6N2E2_30 0.17 -0.6
67,113 + Pf6N2E2_30 0.17 -0.2

Or see this region's nucleotide sequence