Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2681

Experiment: Nitrate reduction, replicate A

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction, replicate A
3,169,296	+				-0.0
3,169,345	+				+0.0
3,169,345	+				+0.4
3,169,346	-				+1.1
3,169,352	-				+0.2
3,169,352	-				-0.2
3,169,473	-				-0.0
3,169,473	-				+2.0
3,169,639	+		Pf6N2E2_2680	0.22	-1.2
3,169,640	-		Pf6N2E2_2680	0.22	+1.3
3,169,640	-		Pf6N2E2_2680	0.22	+0.1
3,169,744	+		Pf6N2E2_2680	0.40	+1.4
3,169,745	-		Pf6N2E2_2680	0.40	-0.6
3,169,745	-		Pf6N2E2_2680	0.40	+1.3
3,169,745	-		Pf6N2E2_2680	0.40	+1.0
3,169,790	+		Pf6N2E2_2680	0.47	+0.0
3,169,790	+		Pf6N2E2_2680	0.47	-0.1
3,169,791	-		Pf6N2E2_2680	0.47	-1.3
3,169,791	-		Pf6N2E2_2680	0.47	-1.0
3,169,816	+		Pf6N2E2_2680	0.52	+0.4
3,169,816	+		Pf6N2E2_2680	0.52	-1.8
3,170,031	-		Pf6N2E2_2680	0.88	-0.0
3,170,112	+				+0.4
3,170,112	+				+2.4
3,170,112	+				-0.6
3,170,112	+				+1.4
3,170,112	+				+1.4
3,170,112	+				+0.4
3,170,113	-				+2.4
3,170,396	+		Pf6N2E2_2681	0.44	+1.4
3,170,396	+		Pf6N2E2_2681	0.44	-0.2
3,170,419	+		Pf6N2E2_2681	0.52	-0.2
3,170,461	+		Pf6N2E2_2681	0.65	+0.4
3,170,461	+		Pf6N2E2_2681	0.65	+1.4
3,170,462	-		Pf6N2E2_2681	0.65	-1.2
3,170,601	+				-0.5
3,170,601	+				+0.3
3,170,602	-				+0.5
3,170,602	-				-0.7
3,170,602	-				-0.2
3,170,735	+		Pf6N2E2_2682	0.12	-0.7
3,170,736	-		Pf6N2E2_2682	0.12	+0.2
3,170,736	-		Pf6N2E2_2682	0.12	+0.0
3,170,770	-		Pf6N2E2_2682	0.16	-0.6
3,170,937	-		Pf6N2E2_2682	0.34	-1.6
3,171,565	+				+0.0
3,171,566	-				+0.8