Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2587 and Pf6N2E2_2588 are separated by 5 nucleotides Pf6N2E2_2588 and Pf6N2E2_2589 are separated by 141 nucleotides Pf6N2E2_2589 and Pf6N2E2_2590 are separated by 6 nucleotides
Pf6N2E2_2587 - Serine phosphatase RsbU, regulator of sigma subunit, at 3,044,211 to 3,045,917
_2587
Pf6N2E2_2588 - Anti-sigma F factor antagonist (spoIIAA-2); Anti-sigma B factor antagonist RsbV, at 3,045,923 to 3,046,189
_2588
Pf6N2E2_2589 - Flagellar protein FliJ, at 3,046,331 to 3,046,780
_2589
Pf6N2E2_2590 - Flagellum-specific ATP synthase FliI, at 3,046,787 to 3,048,145
_2590
Position (kb)
3045
3046
3047 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3044.978 kb on + strand, within Pf6N2E2_2587 at 3044.978 kb on + strand, within Pf6N2E2_2587 at 3044.978 kb on + strand, within Pf6N2E2_2587 at 3044.978 kb on + strand, within Pf6N2E2_2587 at 3044.979 kb on - strand, within Pf6N2E2_2587 at 3044.979 kb on - strand, within Pf6N2E2_2587 at 3044.979 kb on - strand, within Pf6N2E2_2587 at 3044.979 kb on - strand, within Pf6N2E2_2587 at 3044.979 kb on - strand, within Pf6N2E2_2587 at 3044.979 kb on - strand, within Pf6N2E2_2587 at 3044.979 kb on - strand, within Pf6N2E2_2587 at 3045.053 kb on + strand, within Pf6N2E2_2587 at 3045.083 kb on - strand, within Pf6N2E2_2587 at 3045.141 kb on + strand, within Pf6N2E2_2587 at 3045.141 kb on + strand, within Pf6N2E2_2587 at 3045.141 kb on + strand, within Pf6N2E2_2587 at 3045.141 kb on + strand, within Pf6N2E2_2587 at 3045.142 kb on - strand, within Pf6N2E2_2587 at 3045.142 kb on - strand, within Pf6N2E2_2587 at 3045.142 kb on - strand, within Pf6N2E2_2587 at 3045.379 kb on + strand, within Pf6N2E2_2587 at 3045.379 kb on + strand, within Pf6N2E2_2587 at 3045.380 kb on - strand, within Pf6N2E2_2587 at 3045.394 kb on + strand, within Pf6N2E2_2587 at 3045.394 kb on + strand, within Pf6N2E2_2587 at 3045.394 kb on + strand, within Pf6N2E2_2587 at 3045.395 kb on - strand, within Pf6N2E2_2587 at 3045.478 kb on + strand, within Pf6N2E2_2587 at 3045.478 kb on + strand, within Pf6N2E2_2587 at 3045.478 kb on + strand, within Pf6N2E2_2587 at 3045.478 kb on + strand, within Pf6N2E2_2587 at 3045.479 kb on - strand, within Pf6N2E2_2587 at 3045.479 kb on - strand, within Pf6N2E2_2587 at 3045.479 kb on - strand, within Pf6N2E2_2587 at 3045.479 kb on - strand, within Pf6N2E2_2587 at 3045.479 kb on - strand, within Pf6N2E2_2587 at 3045.583 kb on + strand, within Pf6N2E2_2587 at 3045.779 kb on - strand at 3046.034 kb on + strand, within Pf6N2E2_2588 at 3046.035 kb on - strand, within Pf6N2E2_2588 at 3046.035 kb on - strand, within Pf6N2E2_2588 at 3046.071 kb on + strand, within Pf6N2E2_2588 at 3046.072 kb on - strand, within Pf6N2E2_2588 at 3046.072 kb on - strand, within Pf6N2E2_2588 at 3046.252 kb on + strand at 3046.252 kb on + strand at 3046.253 kb on - strand at 3046.253 kb on - strand at 3046.253 kb on - strand at 3046.253 kb on - strand at 3046.253 kb on - strand at 3046.253 kb on - strand at 3046.258 kb on + strand at 3046.258 kb on + strand at 3046.258 kb on + strand at 3046.259 kb on - strand at 3046.259 kb on - strand at 3046.259 kb on - strand at 3046.259 kb on - strand at 3046.259 kb on - strand at 3046.259 kb on - strand at 3046.259 kb on - strand at 3046.267 kb on + strand at 3046.268 kb on - strand at 3046.278 kb on - strand at 3046.278 kb on - strand at 3046.420 kb on + strand, within Pf6N2E2_2589 at 3046.421 kb on - strand, within Pf6N2E2_2589 at 3046.422 kb on + strand, within Pf6N2E2_2589 at 3046.422 kb on + strand, within Pf6N2E2_2589 at 3046.422 kb on + strand, within Pf6N2E2_2589 at 3046.423 kb on - strand, within Pf6N2E2_2589 at 3046.423 kb on - strand, within Pf6N2E2_2589 at 3046.423 kb on - strand, within Pf6N2E2_2589 at 3046.630 kb on - strand, within Pf6N2E2_2589 at 3046.789 kb on - strand at 3046.789 kb on - strand at 3046.813 kb on + strand at 3046.814 kb on - strand at 3046.938 kb on + strand, within Pf6N2E2_2590 at 3046.939 kb on - strand, within Pf6N2E2_2590 at 3046.939 kb on - strand, within Pf6N2E2_2590 at 3046.939 kb on - strand, within Pf6N2E2_2590 at 3046.939 kb on - strand, within Pf6N2E2_2590 at 3046.986 kb on + strand, within Pf6N2E2_2590 at 3046.986 kb on + strand, within Pf6N2E2_2590 at 3046.987 kb on - strand, within Pf6N2E2_2590 at 3046.987 kb on - strand, within Pf6N2E2_2590 at 3047.188 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 3,044,978 + Pf6N2E2_2587 0.45 +1.1 3,044,978 + Pf6N2E2_2587 0.45 +0.1 3,044,978 + Pf6N2E2_2587 0.45 -0.1 3,044,978 + Pf6N2E2_2587 0.45 +0.1 3,044,979 - Pf6N2E2_2587 0.45 -0.3 3,044,979 - Pf6N2E2_2587 0.45 -0.3 3,044,979 - Pf6N2E2_2587 0.45 +0.1 3,044,979 - Pf6N2E2_2587 0.45 +0.8 3,044,979 - Pf6N2E2_2587 0.45 +1.2 3,044,979 - Pf6N2E2_2587 0.45 +0.3 3,044,979 - Pf6N2E2_2587 0.45 -0.4 3,045,053 + Pf6N2E2_2587 0.49 +1.8 3,045,083 - Pf6N2E2_2587 0.51 +0.1 3,045,141 + Pf6N2E2_2587 0.54 -0.4 3,045,141 + Pf6N2E2_2587 0.54 -0.6 3,045,141 + Pf6N2E2_2587 0.54 +0.2 3,045,141 + Pf6N2E2_2587 0.54 -0.7 3,045,142 - Pf6N2E2_2587 0.55 -0.7 3,045,142 - Pf6N2E2_2587 0.55 -1.3 3,045,142 - Pf6N2E2_2587 0.55 +0.4 3,045,379 + Pf6N2E2_2587 0.68 -0.1 3,045,379 + Pf6N2E2_2587 0.68 +0.6 3,045,380 - Pf6N2E2_2587 0.68 -0.4 3,045,394 + Pf6N2E2_2587 0.69 -0.0 3,045,394 + Pf6N2E2_2587 0.69 -0.4 3,045,394 + Pf6N2E2_2587 0.69 -0.5 3,045,395 - Pf6N2E2_2587 0.69 -0.2 3,045,478 + Pf6N2E2_2587 0.74 +1.4 3,045,478 + Pf6N2E2_2587 0.74 -0.2 3,045,478 + Pf6N2E2_2587 0.74 -0.9 3,045,478 + Pf6N2E2_2587 0.74 -0.5 3,045,479 - Pf6N2E2_2587 0.74 +0.2 3,045,479 - Pf6N2E2_2587 0.74 +0.4 3,045,479 - Pf6N2E2_2587 0.74 -1.1 3,045,479 - Pf6N2E2_2587 0.74 -0.4 3,045,479 - Pf6N2E2_2587 0.74 +0.2 3,045,583 + Pf6N2E2_2587 0.80 -0.9 3,045,779 - -0.6 3,046,034 + Pf6N2E2_2588 0.42 +0.4 3,046,035 - Pf6N2E2_2588 0.42 -2.1 3,046,035 - Pf6N2E2_2588 0.42 +0.4 3,046,071 + Pf6N2E2_2588 0.55 -1.2 3,046,072 - Pf6N2E2_2588 0.56 +0.4 3,046,072 - Pf6N2E2_2588 0.56 -0.2 3,046,252 + +1.4 3,046,252 + -0.3 3,046,253 - -0.6 3,046,253 - -1.9 3,046,253 - +0.3 3,046,253 - -1.2 3,046,253 - +0.1 3,046,253 - +1.4 3,046,258 + -0.0 3,046,258 + -1.8 3,046,258 + +0.3 3,046,259 - -0.8 3,046,259 - -1.4 3,046,259 - -0.9 3,046,259 - +0.2 3,046,259 - -0.6 3,046,259 - -1.4 3,046,259 - +0.7 3,046,267 + -0.7 3,046,268 - -1.6 3,046,278 - +1.4 3,046,278 - -0.0 3,046,420 + Pf6N2E2_2589 0.20 +0.8 3,046,421 - Pf6N2E2_2589 0.20 +0.9 3,046,422 + Pf6N2E2_2589 0.20 +0.9 3,046,422 + Pf6N2E2_2589 0.20 +1.0 3,046,422 + Pf6N2E2_2589 0.20 -0.0 3,046,423 - Pf6N2E2_2589 0.20 +0.9 3,046,423 - Pf6N2E2_2589 0.20 +1.0 3,046,423 - Pf6N2E2_2589 0.20 +1.2 3,046,630 - Pf6N2E2_2589 0.66 +0.7 3,046,789 - +0.5 3,046,789 - +0.7 3,046,813 + +1.3 3,046,814 - +0.6 3,046,938 + Pf6N2E2_2590 0.11 +1.2 3,046,939 - Pf6N2E2_2590 0.11 +1.0 3,046,939 - Pf6N2E2_2590 0.11 +0.7 3,046,939 - Pf6N2E2_2590 0.11 +1.3 3,046,939 - Pf6N2E2_2590 0.11 +1.2 3,046,986 + Pf6N2E2_2590 0.15 +0.6 3,046,986 + Pf6N2E2_2590 0.15 +0.9 3,046,987 - Pf6N2E2_2590 0.15 +0.7 3,046,987 - Pf6N2E2_2590 0.15 +1.5 3,047,188 + +2.4
Or see this region's nucleotide sequence