Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2381

Experiment: Nitrate reduction, replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_2380 and Pf6N2E2_2381 are separated by 10 nucleotidesPf6N2E2_2381 and Pf6N2E2_2382 are separated by 197 nucleotides Pf6N2E2_2380 - Nitrate reductase (EC 1.7.99.4) (from data), at 2,817,700 to 2,820,417 _2380 Pf6N2E2_2381 - Uroporphyrinogen-III methyltransferase (EC 2.1.1.107), at 2,820,428 to 2,821,183 _2381 Pf6N2E2_2382 - Major porin and structural outer membrane porin OprF, at 2,821,381 to 2,822,415 _2382 Position (kb) 2820 2821 2822Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2819.730 kb on + strand, within Pf6N2E2_2380at 2819.896 kb on + strand, within Pf6N2E2_2380at 2819.896 kb on + strand, within Pf6N2E2_2380at 2820.031 kb on + strand, within Pf6N2E2_2380at 2820.072 kb on - strand, within Pf6N2E2_2380at 2820.288 kb on + strandat 2820.288 kb on + strandat 2820.427 kb on + strandat 2820.428 kb on - strandat 2820.478 kb on - strandat 2820.478 kb on - strandat 2820.926 kb on + strand, within Pf6N2E2_2381at 2820.926 kb on + strand, within Pf6N2E2_2381at 2820.926 kb on + strand, within Pf6N2E2_2381at 2820.926 kb on + strand, within Pf6N2E2_2381at 2820.926 kb on + strand, within Pf6N2E2_2381at 2820.926 kb on + strand, within Pf6N2E2_2381at 2820.926 kb on + strand, within Pf6N2E2_2381at 2820.927 kb on - strand, within Pf6N2E2_2381at 2820.927 kb on - strand, within Pf6N2E2_2381at 2820.927 kb on - strand, within Pf6N2E2_2381at 2820.927 kb on - strand, within Pf6N2E2_2381at 2820.927 kb on - strand, within Pf6N2E2_2381at 2820.927 kb on - strand, within Pf6N2E2_2381at 2820.927 kb on - strand, within Pf6N2E2_2381at 2821.179 kb on + strandat 2821.179 kb on + strandat 2821.179 kb on + strandat 2821.180 kb on - strandat 2821.291 kb on + strandat 2821.291 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A
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2,819,730 + Pf6N2E2_2380 0.75 -0.4
2,819,896 + Pf6N2E2_2380 0.81 +0.2
2,819,896 + Pf6N2E2_2380 0.81 -0.3
2,820,031 + Pf6N2E2_2380 0.86 +0.4
2,820,072 - Pf6N2E2_2380 0.87 +0.5
2,820,288 + +0.2
2,820,288 + +1.4
2,820,427 + +0.4
2,820,428 - -0.9
2,820,478 - +1.4
2,820,478 - +1.4
2,820,926 + Pf6N2E2_2381 0.66 -1.4
2,820,926 + Pf6N2E2_2381 0.66 +3.0
2,820,926 + Pf6N2E2_2381 0.66 +0.4
2,820,926 + Pf6N2E2_2381 0.66 -1.9
2,820,926 + Pf6N2E2_2381 0.66 +0.8
2,820,926 + Pf6N2E2_2381 0.66 -0.3
2,820,926 + Pf6N2E2_2381 0.66 +0.6
2,820,927 - Pf6N2E2_2381 0.66 +0.4
2,820,927 - Pf6N2E2_2381 0.66 -0.6
2,820,927 - Pf6N2E2_2381 0.66 +1.4
2,820,927 - Pf6N2E2_2381 0.66 -0.2
2,820,927 - Pf6N2E2_2381 0.66 +3.0
2,820,927 - Pf6N2E2_2381 0.66 -1.4
2,820,927 - Pf6N2E2_2381 0.66 +1.4
2,821,179 + -0.5
2,821,179 + -0.9
2,821,179 + -0.2
2,821,180 - +1.4
2,821,291 + +1.0
2,821,291 + +0.4

Or see this region's nucleotide sequence