Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2321 and Pf6N2E2_2322 overlap by 8 nucleotides Pf6N2E2_2322 and Pf6N2E2_2323 overlap by 42 nucleotides Pf6N2E2_2323 and Pf6N2E2_2324 are separated by 76 nucleotides Pf6N2E2_2324 and Pf6N2E2_2325 are separated by 123 nucleotides
Pf6N2E2_2321 - Coproporphyrinogen III oxidase, oxygen-independent (EC 1.3.99.22), at 2,744,233 to 2,745,615
_2321
Pf6N2E2_2322 - hypothetical protein, at 2,745,608 to 2,745,751
_2322
Pf6N2E2_2323 - Fumarate and nitrate reduction regulatory protein, at 2,745,710 to 2,746,444
_2323
Pf6N2E2_2324 - Adenine phosphoribosyltransferase (EC 2.4.2.7), at 2,746,521 to 2,747,069
_2324
Pf6N2E2_2325 - Recombination protein RecR, at 2,747,193 to 2,747,813
_2325
Position (kb)
2745
2746
2747 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2744.866 kb on - strand, within Pf6N2E2_2321 at 2744.968 kb on + strand, within Pf6N2E2_2321 at 2744.969 kb on - strand, within Pf6N2E2_2321 at 2744.969 kb on - strand, within Pf6N2E2_2321 at 2745.094 kb on + strand, within Pf6N2E2_2321 at 2745.095 kb on - strand, within Pf6N2E2_2321 at 2745.095 kb on - strand, within Pf6N2E2_2321 at 2745.156 kb on + strand, within Pf6N2E2_2321 at 2745.156 kb on + strand, within Pf6N2E2_2321 at 2745.280 kb on + strand, within Pf6N2E2_2321 at 2745.439 kb on + strand, within Pf6N2E2_2321 at 2745.439 kb on + strand, within Pf6N2E2_2321 at 2745.571 kb on + strand at 2745.572 kb on - strand at 2745.572 kb on - strand at 2745.610 kb on - strand at 2745.705 kb on - strand, within Pf6N2E2_2322 at 2746.095 kb on + strand, within Pf6N2E2_2323 at 2746.301 kb on + strand, within Pf6N2E2_2323 at 2746.301 kb on + strand, within Pf6N2E2_2323 at 2746.302 kb on - strand, within Pf6N2E2_2323 at 2746.302 kb on - strand, within Pf6N2E2_2323 at 2746.482 kb on - strand at 2746.484 kb on - strand at 2746.484 kb on - strand at 2746.605 kb on - strand, within Pf6N2E2_2324 at 2746.666 kb on - strand, within Pf6N2E2_2324 at 2746.738 kb on - strand, within Pf6N2E2_2324 at 2746.854 kb on - strand, within Pf6N2E2_2324 at 2747.067 kb on + strand at 2747.067 kb on + strand at 2747.067 kb on + strand at 2747.121 kb on - strand at 2747.180 kb on + strand at 2747.180 kb on + strand at 2747.180 kb on + strand at 2747.181 kb on - strand at 2747.181 kb on - strand at 2747.181 kb on - strand at 2747.181 kb on - strand at 2747.188 kb on - strand at 2747.194 kb on + strand at 2747.195 kb on - strand at 2747.333 kb on - strand, within Pf6N2E2_2325 at 2747.333 kb on - strand, within Pf6N2E2_2325 at 2747.333 kb on - strand, within Pf6N2E2_2325 at 2747.333 kb on - strand, within Pf6N2E2_2325 at 2747.333 kb on - strand, within Pf6N2E2_2325
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 2,744,866 - Pf6N2E2_2321 0.46 +1.5 2,744,968 + Pf6N2E2_2321 0.53 +1.6 2,744,969 - Pf6N2E2_2321 0.53 +1.8 2,744,969 - Pf6N2E2_2321 0.53 +2.2 2,745,094 + Pf6N2E2_2321 0.62 +0.8 2,745,095 - Pf6N2E2_2321 0.62 +1.7 2,745,095 - Pf6N2E2_2321 0.62 +1.4 2,745,156 + Pf6N2E2_2321 0.67 +1.5 2,745,156 + Pf6N2E2_2321 0.67 +1.4 2,745,280 + Pf6N2E2_2321 0.76 +1.8 2,745,439 + Pf6N2E2_2321 0.87 +1.0 2,745,439 + Pf6N2E2_2321 0.87 +0.9 2,745,571 + +1.4 2,745,572 - +0.2 2,745,572 - +1.6 2,745,610 - +0.4 2,745,705 - Pf6N2E2_2322 0.67 -0.7 2,746,095 + Pf6N2E2_2323 0.52 -3.0 2,746,301 + Pf6N2E2_2323 0.80 -2.6 2,746,301 + Pf6N2E2_2323 0.80 +0.4 2,746,302 - Pf6N2E2_2323 0.81 -2.8 2,746,302 - Pf6N2E2_2323 0.81 -2.2 2,746,482 - -1.3 2,746,484 - +0.3 2,746,484 - +0.5 2,746,605 - Pf6N2E2_2324 0.15 +0.0 2,746,666 - Pf6N2E2_2324 0.26 -1.1 2,746,738 - Pf6N2E2_2324 0.40 +0.8 2,746,854 - Pf6N2E2_2324 0.61 -0.2 2,747,067 + -0.7 2,747,067 + -1.0 2,747,067 + +0.3 2,747,121 - -0.2 2,747,180 + +1.4 2,747,180 + +0.7 2,747,180 + +0.1 2,747,181 - -1.2 2,747,181 - +0.3 2,747,181 - -0.6 2,747,181 - -0.9 2,747,188 - -0.2 2,747,194 + -0.6 2,747,195 - +0.4 2,747,333 - Pf6N2E2_2325 0.23 +0.0 2,747,333 - Pf6N2E2_2325 0.23 +0.2 2,747,333 - Pf6N2E2_2325 0.23 +0.2 2,747,333 - Pf6N2E2_2325 0.23 -0.3 2,747,333 - Pf6N2E2_2325 0.23 -1.4
Or see this region's nucleotide sequence