Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2224 and Pf6N2E2_2225 are separated by 167 nucleotides Pf6N2E2_2225 and Pf6N2E2_2226 are separated by 71 nucleotides Pf6N2E2_2226 and Pf6N2E2_2227 are separated by 26 nucleotides
Pf6N2E2_2224 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 2,638,044 to 2,638,778
_2224
Pf6N2E2_2225 - FIG00953489: hypothetical protein, at 2,638,946 to 2,639,110
_2225
Pf6N2E2_2226 - Integral membrane protein TerC, at 2,639,182 to 2,639,931
_2226
Pf6N2E2_2227 - Membrane protein involved in colicin uptake, at 2,639,958 to 2,640,209
_2227
Position (kb)
2638
2639
2640 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2637.989 kb on + strand at 2637.989 kb on + strand at 2637.990 kb on - strand at 2638.163 kb on + strand, within Pf6N2E2_2224 at 2638.164 kb on - strand, within Pf6N2E2_2224 at 2638.164 kb on - strand, within Pf6N2E2_2224 at 2638.164 kb on - strand, within Pf6N2E2_2224 at 2638.164 kb on - strand, within Pf6N2E2_2224 at 2638.164 kb on - strand, within Pf6N2E2_2224 at 2638.266 kb on + strand, within Pf6N2E2_2224 at 2638.267 kb on - strand, within Pf6N2E2_2224 at 2638.289 kb on - strand, within Pf6N2E2_2224 at 2638.342 kb on + strand, within Pf6N2E2_2224 at 2638.342 kb on + strand, within Pf6N2E2_2224 at 2638.342 kb on + strand, within Pf6N2E2_2224 at 2638.343 kb on - strand, within Pf6N2E2_2224 at 2638.412 kb on - strand, within Pf6N2E2_2224 at 2638.427 kb on - strand, within Pf6N2E2_2224 at 2638.448 kb on + strand, within Pf6N2E2_2224 at 2638.449 kb on - strand, within Pf6N2E2_2224 at 2638.449 kb on - strand, within Pf6N2E2_2224 at 2638.449 kb on - strand, within Pf6N2E2_2224 at 2638.567 kb on + strand, within Pf6N2E2_2224 at 2638.568 kb on - strand, within Pf6N2E2_2224 at 2638.599 kb on - strand, within Pf6N2E2_2224 at 2638.626 kb on + strand, within Pf6N2E2_2224 at 2638.627 kb on - strand, within Pf6N2E2_2224 at 2638.627 kb on - strand, within Pf6N2E2_2224 at 2638.709 kb on + strand at 2638.778 kb on + strand at 2638.778 kb on + strand at 2638.779 kb on - strand at 2638.800 kb on - strand at 2638.800 kb on - strand at 2638.830 kb on + strand at 2638.831 kb on - strand at 2638.831 kb on - strand at 2638.890 kb on + strand at 2638.890 kb on + strand at 2638.890 kb on + strand at 2638.890 kb on + strand at 2638.891 kb on - strand at 2638.903 kb on + strand at 2638.903 kb on + strand at 2638.903 kb on + strand at 2638.903 kb on + strand at 2638.903 kb on + strand at 2638.903 kb on + strand at 2638.904 kb on - strand at 2638.904 kb on - strand at 2638.904 kb on - strand at 2638.904 kb on - strand at 2638.904 kb on - strand at 2638.906 kb on + strand at 2638.906 kb on + strand at 2638.907 kb on - strand at 2638.907 kb on - strand at 2638.977 kb on + strand, within Pf6N2E2_2225 at 2638.977 kb on + strand, within Pf6N2E2_2225 at 2638.978 kb on - strand, within Pf6N2E2_2225 at 2639.270 kb on + strand, within Pf6N2E2_2226 at 2639.270 kb on + strand, within Pf6N2E2_2226 at 2639.271 kb on - strand, within Pf6N2E2_2226 at 2639.271 kb on - strand, within Pf6N2E2_2226 at 2639.271 kb on - strand, within Pf6N2E2_2226 at 2639.274 kb on + strand, within Pf6N2E2_2226 at 2639.274 kb on + strand, within Pf6N2E2_2226 at 2639.274 kb on + strand, within Pf6N2E2_2226 at 2639.274 kb on + strand, within Pf6N2E2_2226 at 2639.274 kb on + strand, within Pf6N2E2_2226 at 2639.275 kb on - strand, within Pf6N2E2_2226 at 2639.275 kb on - strand, within Pf6N2E2_2226 at 2639.275 kb on - strand, within Pf6N2E2_2226 at 2639.275 kb on - strand, within Pf6N2E2_2226 at 2639.275 kb on - strand, within Pf6N2E2_2226 at 2639.275 kb on - strand, within Pf6N2E2_2226 at 2639.275 kb on - strand, within Pf6N2E2_2226 at 2639.276 kb on + strand, within Pf6N2E2_2226 at 2639.276 kb on + strand, within Pf6N2E2_2226 at 2639.277 kb on - strand, within Pf6N2E2_2226 at 2639.277 kb on - strand, within Pf6N2E2_2226 at 2639.277 kb on - strand, within Pf6N2E2_2226 at 2639.277 kb on - strand, within Pf6N2E2_2226 at 2639.277 kb on - strand, within Pf6N2E2_2226 at 2639.277 kb on - strand, within Pf6N2E2_2226 at 2639.277 kb on - strand, within Pf6N2E2_2226 at 2639.917 kb on + strand at 2639.917 kb on + strand at 2639.918 kb on - strand at 2639.924 kb on + strand at 2639.924 kb on + strand at 2639.924 kb on + strand at 2639.924 kb on + strand at 2639.925 kb on - strand at 2639.925 kb on - strand at 2639.925 kb on - strand at 2639.925 kb on - strand at 2639.925 kb on - strand at 2639.959 kb on + strand at 2639.959 kb on + strand at 2639.959 kb on + strand at 2639.959 kb on + strand at 2639.960 kb on - strand at 2639.960 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 2,637,989 + -0.8 2,637,989 + -0.4 2,637,990 - +2.4 2,638,163 + Pf6N2E2_2224 0.16 -0.8 2,638,164 - Pf6N2E2_2224 0.16 -1.0 2,638,164 - Pf6N2E2_2224 0.16 -0.7 2,638,164 - Pf6N2E2_2224 0.16 +0.0 2,638,164 - Pf6N2E2_2224 0.16 +2.0 2,638,164 - Pf6N2E2_2224 0.16 -0.1 2,638,266 + Pf6N2E2_2224 0.30 -0.2 2,638,267 - Pf6N2E2_2224 0.30 -0.7 2,638,289 - Pf6N2E2_2224 0.33 -1.2 2,638,342 + Pf6N2E2_2224 0.41 +0.4 2,638,342 + Pf6N2E2_2224 0.41 +0.4 2,638,342 + Pf6N2E2_2224 0.41 +0.1 2,638,343 - Pf6N2E2_2224 0.41 +0.4 2,638,412 - Pf6N2E2_2224 0.50 -0.7 2,638,427 - Pf6N2E2_2224 0.52 +0.7 2,638,448 + Pf6N2E2_2224 0.55 -0.7 2,638,449 - Pf6N2E2_2224 0.55 -0.6 2,638,449 - Pf6N2E2_2224 0.55 +0.0 2,638,449 - Pf6N2E2_2224 0.55 +0.5 2,638,567 + Pf6N2E2_2224 0.71 -0.9 2,638,568 - Pf6N2E2_2224 0.71 -1.2 2,638,599 - Pf6N2E2_2224 0.76 -1.5 2,638,626 + Pf6N2E2_2224 0.79 -1.0 2,638,627 - Pf6N2E2_2224 0.79 +0.1 2,638,627 - Pf6N2E2_2224 0.79 -1.1 2,638,709 + -0.7 2,638,778 + -0.2 2,638,778 + -0.9 2,638,779 - +0.3 2,638,800 - +0.4 2,638,800 - +0.3 2,638,830 + -0.6 2,638,831 - -0.2 2,638,831 - -0.7 2,638,890 + +0.1 2,638,890 + -0.7 2,638,890 + -0.8 2,638,890 + -1.0 2,638,891 - -1.7 2,638,903 + -1.0 2,638,903 + -0.4 2,638,903 + -1.8 2,638,903 + -1.0 2,638,903 + -0.8 2,638,903 + -1.3 2,638,904 - +0.2 2,638,904 - +1.4 2,638,904 - +1.4 2,638,904 - +0.6 2,638,904 - -0.6 2,638,906 + -2.3 2,638,906 + -1.4 2,638,907 - -0.9 2,638,907 - +0.8 2,638,977 + Pf6N2E2_2225 0.19 +0.1 2,638,977 + Pf6N2E2_2225 0.19 -2.0 2,638,978 - Pf6N2E2_2225 0.19 -0.2 2,639,270 + Pf6N2E2_2226 0.12 +2.0 2,639,270 + Pf6N2E2_2226 0.12 -0.4 2,639,271 - Pf6N2E2_2226 0.12 +0.3 2,639,271 - Pf6N2E2_2226 0.12 -0.3 2,639,271 - Pf6N2E2_2226 0.12 +2.4 2,639,274 + Pf6N2E2_2226 0.12 +1.1 2,639,274 + Pf6N2E2_2226 0.12 +1.4 2,639,274 + Pf6N2E2_2226 0.12 +0.1 2,639,274 + Pf6N2E2_2226 0.12 +2.4 2,639,274 + Pf6N2E2_2226 0.12 +0.3 2,639,275 - Pf6N2E2_2226 0.12 -0.7 2,639,275 - Pf6N2E2_2226 0.12 +0.1 2,639,275 - Pf6N2E2_2226 0.12 -0.2 2,639,275 - Pf6N2E2_2226 0.12 -0.7 2,639,275 - Pf6N2E2_2226 0.12 +0.0 2,639,275 - Pf6N2E2_2226 0.12 -0.2 2,639,275 - Pf6N2E2_2226 0.12 -0.0 2,639,276 + Pf6N2E2_2226 0.13 -0.3 2,639,276 + Pf6N2E2_2226 0.13 -0.1 2,639,277 - Pf6N2E2_2226 0.13 -0.9 2,639,277 - Pf6N2E2_2226 0.13 +0.5 2,639,277 - Pf6N2E2_2226 0.13 -0.6 2,639,277 - Pf6N2E2_2226 0.13 +0.0 2,639,277 - Pf6N2E2_2226 0.13 +0.5 2,639,277 - Pf6N2E2_2226 0.13 -0.2 2,639,277 - Pf6N2E2_2226 0.13 +0.7 2,639,917 + -1.0 2,639,917 + +1.4 2,639,918 - -0.7 2,639,924 + +1.0 2,639,924 + -1.2 2,639,924 + -1.2 2,639,924 + +0.4 2,639,925 - -2.1 2,639,925 - +0.3 2,639,925 - +1.4 2,639,925 - +0.4 2,639,925 - +0.0 2,639,959 + -0.6 2,639,959 + +0.4 2,639,959 + +0.8 2,639,959 + -0.7 2,639,960 - -0.1 2,639,960 - -1.4
Or see this region's nucleotide sequence