Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2016 and Pf6N2E2_2017 are separated by 164 nucleotides Pf6N2E2_2017 and Pf6N2E2_2018 are separated by 1 nucleotides Pf6N2E2_2018 and Pf6N2E2_2019 are separated by 26 nucleotides
Pf6N2E2_2016 - hypothetical protein, at 2,401,994 to 2,404,036
_2016
Pf6N2E2_2017 - DNA-binding protein, at 2,404,201 to 2,404,482
_2017
Pf6N2E2_2018 - hypothetical protein, at 2,404,484 to 2,404,630
_2018
Pf6N2E2_2019 - hypothetical protein, at 2,404,657 to 2,408,535
_2019
Position (kb)
2404
2405 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2403.267 kb on - strand, within Pf6N2E2_2016 at 2403.344 kb on + strand, within Pf6N2E2_2016 at 2403.345 kb on - strand, within Pf6N2E2_2016 at 2403.400 kb on + strand, within Pf6N2E2_2016 at 2403.400 kb on + strand, within Pf6N2E2_2016 at 2403.418 kb on + strand, within Pf6N2E2_2016 at 2403.421 kb on + strand, within Pf6N2E2_2016 at 2403.422 kb on - strand, within Pf6N2E2_2016 at 2403.422 kb on - strand, within Pf6N2E2_2016 at 2403.545 kb on + strand, within Pf6N2E2_2016 at 2403.545 kb on + strand, within Pf6N2E2_2016 at 2403.546 kb on - strand, within Pf6N2E2_2016 at 2403.546 kb on - strand, within Pf6N2E2_2016 at 2403.675 kb on - strand, within Pf6N2E2_2016 at 2403.675 kb on - strand, within Pf6N2E2_2016 at 2403.703 kb on + strand, within Pf6N2E2_2016 at 2403.710 kb on + strand, within Pf6N2E2_2016 at 2403.710 kb on + strand, within Pf6N2E2_2016 at 2403.710 kb on + strand, within Pf6N2E2_2016 at 2403.711 kb on - strand, within Pf6N2E2_2016 at 2403.711 kb on - strand, within Pf6N2E2_2016 at 2403.711 kb on - strand, within Pf6N2E2_2016 at 2403.711 kb on - strand, within Pf6N2E2_2016 at 2403.711 kb on - strand, within Pf6N2E2_2016 at 2403.711 kb on - strand, within Pf6N2E2_2016 at 2403.724 kb on + strand, within Pf6N2E2_2016 at 2403.730 kb on + strand, within Pf6N2E2_2016 at 2403.730 kb on + strand, within Pf6N2E2_2016 at 2403.730 kb on + strand, within Pf6N2E2_2016 at 2403.731 kb on - strand, within Pf6N2E2_2016 at 2403.731 kb on - strand, within Pf6N2E2_2016 at 2403.731 kb on - strand, within Pf6N2E2_2016 at 2403.731 kb on - strand, within Pf6N2E2_2016 at 2403.731 kb on - strand, within Pf6N2E2_2016 at 2403.731 kb on - strand, within Pf6N2E2_2016 at 2403.812 kb on - strand, within Pf6N2E2_2016 at 2404.014 kb on + strand at 2404.014 kb on + strand at 2404.014 kb on + strand at 2404.014 kb on + strand at 2404.014 kb on + strand at 2404.014 kb on + strand at 2404.014 kb on + strand at 2404.014 kb on + strand at 2404.015 kb on - strand at 2404.015 kb on - strand at 2404.015 kb on - strand at 2404.015 kb on - strand at 2404.015 kb on - strand at 2404.015 kb on - strand at 2404.041 kb on + strand at 2404.045 kb on + strand at 2404.045 kb on + strand at 2404.046 kb on - strand at 2404.046 kb on - strand at 2404.046 kb on - strand at 2404.064 kb on + strand at 2404.065 kb on - strand at 2404.140 kb on + strand at 2404.140 kb on + strand at 2404.140 kb on + strand at 2404.141 kb on - strand at 2404.141 kb on - strand at 2404.141 kb on - strand at 2404.141 kb on - strand at 2404.141 kb on - strand at 2404.141 kb on - strand at 2404.144 kb on + strand at 2404.144 kb on + strand at 2404.145 kb on - strand at 2404.145 kb on - strand at 2404.271 kb on + strand, within Pf6N2E2_2017 at 2404.449 kb on + strand, within Pf6N2E2_2017 at 2404.450 kb on - strand, within Pf6N2E2_2017 at 2404.474 kb on + strand at 2404.485 kb on + strand at 2404.485 kb on + strand at 2404.486 kb on - strand at 2404.486 kb on - strand at 2404.570 kb on + strand, within Pf6N2E2_2018 at 2404.571 kb on - strand, within Pf6N2E2_2018 at 2404.630 kb on + strand at 2404.631 kb on - strand at 2404.631 kb on - strand at 2404.654 kb on + strand at 2404.654 kb on + strand at 2404.654 kb on + strand at 2404.654 kb on + strand at 2404.654 kb on + strand at 2404.654 kb on + strand at 2404.655 kb on - strand at 2404.665 kb on + strand at 2404.665 kb on + strand at 2404.665 kb on + strand at 2404.665 kb on + strand at 2404.666 kb on - strand at 2404.695 kb on - strand at 2404.695 kb on - strand at 2404.695 kb on - strand at 2404.701 kb on + strand at 2404.702 kb on - strand at 2404.702 kb on - strand at 2404.732 kb on - strand at 2404.770 kb on + strand at 2404.770 kb on + strand at 2404.770 kb on + strand at 2404.861 kb on + strand at 2404.861 kb on + strand at 2404.861 kb on + strand at 2404.862 kb on - strand at 2404.862 kb on - strand at 2404.862 kb on - strand at 2404.867 kb on + strand at 2404.867 kb on + strand at 2404.868 kb on - strand at 2404.868 kb on - strand at 2404.924 kb on + strand at 2404.924 kb on + strand at 2404.925 kb on - strand at 2404.928 kb on + strand at 2404.928 kb on + strand at 2404.928 kb on + strand at 2404.929 kb on - strand at 2404.929 kb on - strand at 2404.976 kb on + strand at 2404.976 kb on + strand at 2404.977 kb on - strand at 2405.092 kb on - strand, within Pf6N2E2_2019 at 2405.108 kb on + strand, within Pf6N2E2_2019 at 2405.108 kb on + strand, within Pf6N2E2_2019 at 2405.108 kb on + strand, within Pf6N2E2_2019 at 2405.108 kb on + strand, within Pf6N2E2_2019 at 2405.109 kb on - strand, within Pf6N2E2_2019 at 2405.109 kb on - strand, within Pf6N2E2_2019 at 2405.109 kb on - strand, within Pf6N2E2_2019 at 2405.117 kb on + strand, within Pf6N2E2_2019 at 2405.118 kb on - strand, within Pf6N2E2_2019 at 2405.118 kb on - strand, within Pf6N2E2_2019 at 2405.280 kb on - strand, within Pf6N2E2_2019 at 2405.280 kb on - strand, within Pf6N2E2_2019 at 2405.282 kb on + strand, within Pf6N2E2_2019 at 2405.283 kb on - strand, within Pf6N2E2_2019 at 2405.283 kb on - strand, within Pf6N2E2_2019 at 2405.286 kb on + strand, within Pf6N2E2_2019 at 2405.287 kb on - strand, within Pf6N2E2_2019 at 2405.287 kb on - strand, within Pf6N2E2_2019 at 2405.304 kb on + strand, within Pf6N2E2_2019 at 2405.440 kb on + strand, within Pf6N2E2_2019 at 2405.441 kb on - strand, within Pf6N2E2_2019 at 2405.441 kb on - strand, within Pf6N2E2_2019
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 2,403,267 - Pf6N2E2_2016 0.62 -1.1 2,403,344 + Pf6N2E2_2016 0.66 +0.7 2,403,345 - Pf6N2E2_2016 0.66 -0.1 2,403,400 + Pf6N2E2_2016 0.69 +0.7 2,403,400 + Pf6N2E2_2016 0.69 -0.2 2,403,418 + Pf6N2E2_2016 0.70 -0.9 2,403,421 + Pf6N2E2_2016 0.70 -0.7 2,403,422 - Pf6N2E2_2016 0.70 +0.6 2,403,422 - Pf6N2E2_2016 0.70 -0.6 2,403,545 + Pf6N2E2_2016 0.76 +0.5 2,403,545 + Pf6N2E2_2016 0.76 +0.3 2,403,546 - Pf6N2E2_2016 0.76 -0.2 2,403,546 - Pf6N2E2_2016 0.76 -1.6 2,403,675 - Pf6N2E2_2016 0.82 -0.4 2,403,675 - Pf6N2E2_2016 0.82 +1.5 2,403,703 + Pf6N2E2_2016 0.84 -0.2 2,403,710 + Pf6N2E2_2016 0.84 -2.8 2,403,710 + Pf6N2E2_2016 0.84 -0.6 2,403,710 + Pf6N2E2_2016 0.84 +0.5 2,403,711 - Pf6N2E2_2016 0.84 +1.5 2,403,711 - Pf6N2E2_2016 0.84 -0.9 2,403,711 - Pf6N2E2_2016 0.84 +1.5 2,403,711 - Pf6N2E2_2016 0.84 +1.1 2,403,711 - Pf6N2E2_2016 0.84 +0.5 2,403,711 - Pf6N2E2_2016 0.84 +0.1 2,403,724 + Pf6N2E2_2016 0.85 -0.5 2,403,730 + Pf6N2E2_2016 0.85 -0.3 2,403,730 + Pf6N2E2_2016 0.85 -0.4 2,403,730 + Pf6N2E2_2016 0.85 +1.3 2,403,731 - Pf6N2E2_2016 0.85 +0.6 2,403,731 - Pf6N2E2_2016 0.85 -0.5 2,403,731 - Pf6N2E2_2016 0.85 +1.5 2,403,731 - Pf6N2E2_2016 0.85 +0.4 2,403,731 - Pf6N2E2_2016 0.85 -0.4 2,403,731 - Pf6N2E2_2016 0.85 +0.1 2,403,812 - Pf6N2E2_2016 0.89 +1.5 2,404,014 + -0.2 2,404,014 + -1.3 2,404,014 + +0.1 2,404,014 + -0.1 2,404,014 + -0.4 2,404,014 + +1.1 2,404,014 + +1.2 2,404,014 + +0.6 2,404,015 - +2.5 2,404,015 - +0.5 2,404,015 - +1.5 2,404,015 - +0.5 2,404,015 - -3.4 2,404,015 - -0.4 2,404,041 + +0.1 2,404,045 + +1.7 2,404,045 + -0.2 2,404,046 - +0.1 2,404,046 - +0.6 2,404,046 - +0.8 2,404,064 + +0.7 2,404,065 - +0.6 2,404,140 + +1.3 2,404,140 + +0.1 2,404,140 + -0.2 2,404,141 - -0.3 2,404,141 - -0.6 2,404,141 - +0.2 2,404,141 - -1.4 2,404,141 - +0.0 2,404,141 - -0.8 2,404,144 + +0.6 2,404,144 + -0.1 2,404,145 - -0.2 2,404,145 - +0.5 2,404,271 + Pf6N2E2_2017 0.25 -0.8 2,404,449 + Pf6N2E2_2017 0.88 -1.2 2,404,450 - Pf6N2E2_2017 0.88 -0.1 2,404,474 + +0.1 2,404,485 + -0.2 2,404,485 + -0.1 2,404,486 - +1.1 2,404,486 - +0.1 2,404,570 + Pf6N2E2_2018 0.59 -1.1 2,404,571 - Pf6N2E2_2018 0.59 -0.8 2,404,630 + +0.8 2,404,631 - -1.3 2,404,631 - +0.1 2,404,654 + -0.2 2,404,654 + -0.2 2,404,654 + +0.6 2,404,654 + +1.5 2,404,654 + +0.6 2,404,654 + +0.2 2,404,655 - +0.8 2,404,665 + +1.5 2,404,665 + +0.5 2,404,665 + -0.8 2,404,665 + +0.4 2,404,666 - +0.4 2,404,695 - +0.3 2,404,695 - -0.4 2,404,695 - +0.5 2,404,701 + -0.9 2,404,702 - +0.4 2,404,702 - +2.5 2,404,732 - -0.9 2,404,770 + +1.5 2,404,770 + -1.1 2,404,770 + -0.6 2,404,861 + +0.4 2,404,861 + -0.1 2,404,861 + -0.3 2,404,862 - +0.8 2,404,862 - -0.0 2,404,862 - +0.7 2,404,867 + +0.9 2,404,867 + -1.1 2,404,868 - -3.2 2,404,868 - -2.0 2,404,924 + -0.8 2,404,924 + -0.7 2,404,925 - +0.1 2,404,928 + -0.7 2,404,928 + +0.6 2,404,928 + -1.0 2,404,929 - -0.5 2,404,929 - +0.6 2,404,976 + -1.4 2,404,976 + -0.0 2,404,977 - -1.9 2,405,092 - Pf6N2E2_2019 0.11 +0.5 2,405,108 + Pf6N2E2_2019 0.12 +0.9 2,405,108 + Pf6N2E2_2019 0.12 -1.3 2,405,108 + Pf6N2E2_2019 0.12 +0.9 2,405,108 + Pf6N2E2_2019 0.12 +0.0 2,405,109 - Pf6N2E2_2019 0.12 -0.8 2,405,109 - Pf6N2E2_2019 0.12 -0.3 2,405,109 - Pf6N2E2_2019 0.12 +0.8 2,405,117 + Pf6N2E2_2019 0.12 -0.0 2,405,118 - Pf6N2E2_2019 0.12 -0.1 2,405,118 - Pf6N2E2_2019 0.12 +0.5 2,405,280 - Pf6N2E2_2019 0.16 -0.4 2,405,280 - Pf6N2E2_2019 0.16 +1.5 2,405,282 + Pf6N2E2_2019 0.16 -0.8 2,405,283 - Pf6N2E2_2019 0.16 +0.9 2,405,283 - Pf6N2E2_2019 0.16 +0.3 2,405,286 + Pf6N2E2_2019 0.16 +0.4 2,405,287 - Pf6N2E2_2019 0.16 -0.3 2,405,287 - Pf6N2E2_2019 0.16 -0.3 2,405,304 + Pf6N2E2_2019 0.17 -1.0 2,405,440 + Pf6N2E2_2019 0.20 -0.9 2,405,441 - Pf6N2E2_2019 0.20 -0.7 2,405,441 - Pf6N2E2_2019 0.20 -0.2
Or see this region's nucleotide sequence