Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1652

Experiment: Nitrate reduction, replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1651 and Pf6N2E2_1652 are separated by 48 nucleotidesPf6N2E2_1652 and Pf6N2E2_1653 are separated by 169 nucleotides Pf6N2E2_1651 - Sucrose specific transcriptional regulator CscR, LacI family, at 1,957,429 to 1,958,412 _1651 Pf6N2E2_1652 - Transcriptional regulator, TetR family, at 1,958,461 to 1,959,048 _1652 Pf6N2E2_1653 - NADH:flavin oxidoreductase/NADH oxidase, at 1,959,218 to 1,960,312 _1653 Position (kb) 1958 1959 1960Strain fitness (log2 ratio) -2 -1 0 1at 1958.410 kb on + strandat 1958.410 kb on + strandat 1958.410 kb on + strandat 1958.411 kb on - strandat 1958.439 kb on + strandat 1958.440 kb on - strandat 1958.462 kb on + strandat 1958.462 kb on + strandat 1958.796 kb on - strand, within Pf6N2E2_1652at 1958.796 kb on - strand, within Pf6N2E2_1652at 1958.796 kb on - strand, within Pf6N2E2_1652at 1958.816 kb on - strand, within Pf6N2E2_1652at 1959.245 kb on + strandat 1959.245 kb on + strandat 1959.246 kb on - strandat 1959.302 kb on - strandat 1959.347 kb on + strand, within Pf6N2E2_1653at 1959.348 kb on - strand, within Pf6N2E2_1653at 1959.350 kb on + strand, within Pf6N2E2_1653at 1959.351 kb on - strand, within Pf6N2E2_1653at 1959.420 kb on - strand, within Pf6N2E2_1653at 1959.440 kb on + strand, within Pf6N2E2_1653at 1959.610 kb on - strand, within Pf6N2E2_1653at 1959.736 kb on - strand, within Pf6N2E2_1653

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A
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1,958,410 + -0.4
1,958,410 + +1.4
1,958,410 + -0.6
1,958,411 - -0.3
1,958,439 + -0.2
1,958,440 - +0.0
1,958,462 + -1.1
1,958,462 + -1.8
1,958,796 - Pf6N2E2_1652 0.57 -0.6
1,958,796 - Pf6N2E2_1652 0.57 -0.4
1,958,796 - Pf6N2E2_1652 0.57 -1.7
1,958,816 - Pf6N2E2_1652 0.60 -0.3
1,959,245 + -0.5
1,959,245 + -1.9
1,959,246 - -1.1
1,959,302 - +0.6
1,959,347 + Pf6N2E2_1653 0.12 +0.5
1,959,348 - Pf6N2E2_1653 0.12 +0.2
1,959,350 + Pf6N2E2_1653 0.12 +0.3
1,959,351 - Pf6N2E2_1653 0.12 -0.2
1,959,420 - Pf6N2E2_1653 0.18 -0.6
1,959,440 + Pf6N2E2_1653 0.20 -0.9
1,959,610 - Pf6N2E2_1653 0.36 +0.7
1,959,736 - Pf6N2E2_1653 0.47 -1.6

Or see this region's nucleotide sequence