Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1594 and Pf6N2E2_1595 are separated by 196 nucleotides Pf6N2E2_1595 and Pf6N2E2_1596 are separated by 37 nucleotides Pf6N2E2_1596 and Pf6N2E2_1597 overlap by 4 nucleotides Pf6N2E2_1597 and Pf6N2E2_1598 are separated by 11 nucleotides Pf6N2E2_1598 and Pf6N2E2_1599 are separated by 9 nucleotides
Pf6N2E2_1594 - Tia invasion determinant-related protein precursor, at 1,867,125 to 1,867,823
_1594
Pf6N2E2_1595 - Periplasmic nitrate reductase component NapE, at 1,868,020 to 1,868,187
_1595
Pf6N2E2_1596 - Periplasmic nitrate reductase component NapD, at 1,868,225 to 1,868,485
_1596
Pf6N2E2_1597 - Periplasmic nitrate reductase precursor (EC 1.7.99.4), at 1,868,482 to 1,870,986
_1597
Pf6N2E2_1598 - Nitrate reductase cytochrome c550-type subunit, at 1,870,998 to 1,871,483
_1598
Pf6N2E2_1599 - Cytochrome c-type protein NapC, at 1,871,493 to 1,872,089
_1599
Position (kb)
1868
1869
1870
1871 Strain fitness (log2 ratio)
-2
-1
0
1 at 1867.500 kb on + strand, within Pf6N2E2_1594 at 1867.501 kb on - strand, within Pf6N2E2_1594 at 1867.545 kb on + strand, within Pf6N2E2_1594 at 1867.545 kb on + strand, within Pf6N2E2_1594 at 1867.545 kb on + strand, within Pf6N2E2_1594 at 1867.545 kb on + strand, within Pf6N2E2_1594 at 1867.545 kb on + strand, within Pf6N2E2_1594 at 1867.545 kb on + strand, within Pf6N2E2_1594 at 1867.546 kb on - strand, within Pf6N2E2_1594 at 1867.546 kb on - strand, within Pf6N2E2_1594 at 1867.546 kb on - strand, within Pf6N2E2_1594 at 1867.711 kb on - strand, within Pf6N2E2_1594 at 1867.779 kb on + strand at 1867.812 kb on + strand at 1867.821 kb on + strand at 1867.821 kb on + strand at 1867.821 kb on + strand at 1867.821 kb on + strand at 1867.821 kb on + strand at 1867.952 kb on + strand at 1867.953 kb on - strand at 1867.991 kb on - strand at 1868.162 kb on - strand, within Pf6N2E2_1595 at 1868.162 kb on - strand, within Pf6N2E2_1595 at 1868.211 kb on - strand at 1868.243 kb on - strand at 1868.243 kb on - strand at 1868.243 kb on - strand at 1868.259 kb on + strand, within Pf6N2E2_1596 at 1868.260 kb on - strand, within Pf6N2E2_1596 at 1868.761 kb on + strand, within Pf6N2E2_1597 at 1868.761 kb on + strand, within Pf6N2E2_1597 at 1868.761 kb on + strand, within Pf6N2E2_1597 at 1868.761 kb on + strand, within Pf6N2E2_1597 at 1868.762 kb on - strand, within Pf6N2E2_1597 at 1868.762 kb on - strand, within Pf6N2E2_1597 at 1869.116 kb on + strand, within Pf6N2E2_1597 at 1869.117 kb on - strand, within Pf6N2E2_1597 at 1869.204 kb on - strand, within Pf6N2E2_1597 at 1869.296 kb on - strand, within Pf6N2E2_1597 at 1869.296 kb on - strand, within Pf6N2E2_1597 at 1869.296 kb on - strand, within Pf6N2E2_1597 at 1869.296 kb on - strand, within Pf6N2E2_1597 at 1869.565 kb on + strand, within Pf6N2E2_1597 at 1869.653 kb on - strand, within Pf6N2E2_1597 at 1869.653 kb on - strand, within Pf6N2E2_1597 at 1869.653 kb on - strand, within Pf6N2E2_1597 at 1869.653 kb on - strand, within Pf6N2E2_1597 at 1869.736 kb on - strand, within Pf6N2E2_1597 at 1869.937 kb on - strand, within Pf6N2E2_1597 at 1870.021 kb on + strand, within Pf6N2E2_1597 at 1870.254 kb on + strand, within Pf6N2E2_1597 at 1870.328 kb on - strand, within Pf6N2E2_1597 at 1870.387 kb on + strand, within Pf6N2E2_1597 at 1870.388 kb on - strand, within Pf6N2E2_1597 at 1870.520 kb on - strand, within Pf6N2E2_1597 at 1870.549 kb on + strand, within Pf6N2E2_1597 at 1870.624 kb on + strand, within Pf6N2E2_1597 at 1870.729 kb on + strand, within Pf6N2E2_1597 at 1870.865 kb on + strand at 1870.866 kb on - strand at 1871.184 kb on + strand, within Pf6N2E2_1598 at 1871.319 kb on + strand, within Pf6N2E2_1598 at 1871.320 kb on - strand, within Pf6N2E2_1598 at 1871.368 kb on - strand, within Pf6N2E2_1598 at 1871.404 kb on + strand, within Pf6N2E2_1598 at 1871.404 kb on + strand, within Pf6N2E2_1598 at 1871.405 kb on - strand, within Pf6N2E2_1598 at 1871.405 kb on - strand, within Pf6N2E2_1598 at 1871.524 kb on - strand at 1871.553 kb on + strand, within Pf6N2E2_1599 at 1871.554 kb on - strand, within Pf6N2E2_1599 at 1871.554 kb on - strand, within Pf6N2E2_1599 at 1871.554 kb on - strand, within Pf6N2E2_1599 at 1871.554 kb on - strand, within Pf6N2E2_1599 at 1871.554 kb on - strand, within Pf6N2E2_1599 at 1871.554 kb on - strand, within Pf6N2E2_1599 at 1871.554 kb on - strand, within Pf6N2E2_1599 at 1871.557 kb on - strand, within Pf6N2E2_1599 at 1871.557 kb on - strand, within Pf6N2E2_1599 at 1871.715 kb on + strand, within Pf6N2E2_1599 at 1871.781 kb on - strand, within Pf6N2E2_1599
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 1,867,500 + Pf6N2E2_1594 0.54 +1.0 1,867,501 - Pf6N2E2_1594 0.54 -0.3 1,867,545 + Pf6N2E2_1594 0.60 -0.8 1,867,545 + Pf6N2E2_1594 0.60 +0.2 1,867,545 + Pf6N2E2_1594 0.60 -0.6 1,867,545 + Pf6N2E2_1594 0.60 -0.9 1,867,545 + Pf6N2E2_1594 0.60 -0.6 1,867,545 + Pf6N2E2_1594 0.60 +0.2 1,867,546 - Pf6N2E2_1594 0.60 +0.3 1,867,546 - Pf6N2E2_1594 0.60 +1.4 1,867,546 - Pf6N2E2_1594 0.60 +0.4 1,867,711 - Pf6N2E2_1594 0.84 -0.4 1,867,779 + -0.6 1,867,812 + +0.1 1,867,821 + +0.0 1,867,821 + -0.4 1,867,821 + -0.5 1,867,821 + +1.4 1,867,821 + +0.0 1,867,952 + +0.2 1,867,953 - -0.4 1,867,991 - -0.0 1,868,162 - Pf6N2E2_1595 0.85 +0.1 1,868,162 - Pf6N2E2_1595 0.85 +1.4 1,868,211 - -0.2 1,868,243 - -1.1 1,868,243 - +1.4 1,868,243 - -0.6 1,868,259 + Pf6N2E2_1596 0.13 +0.9 1,868,260 - Pf6N2E2_1596 0.13 -0.9 1,868,761 + Pf6N2E2_1597 0.11 -0.4 1,868,761 + Pf6N2E2_1597 0.11 -0.2 1,868,761 + Pf6N2E2_1597 0.11 -1.3 1,868,761 + Pf6N2E2_1597 0.11 -0.6 1,868,762 - Pf6N2E2_1597 0.11 -0.3 1,868,762 - Pf6N2E2_1597 0.11 -0.1 1,869,116 + Pf6N2E2_1597 0.25 +1.4 1,869,117 - Pf6N2E2_1597 0.25 +1.4 1,869,204 - Pf6N2E2_1597 0.29 -0.2 1,869,296 - Pf6N2E2_1597 0.32 +0.5 1,869,296 - Pf6N2E2_1597 0.32 -0.3 1,869,296 - Pf6N2E2_1597 0.32 +0.5 1,869,296 - Pf6N2E2_1597 0.32 +0.3 1,869,565 + Pf6N2E2_1597 0.43 -0.2 1,869,653 - Pf6N2E2_1597 0.47 +0.6 1,869,653 - Pf6N2E2_1597 0.47 +0.6 1,869,653 - Pf6N2E2_1597 0.47 -0.6 1,869,653 - Pf6N2E2_1597 0.47 -0.9 1,869,736 - Pf6N2E2_1597 0.50 -1.1 1,869,937 - Pf6N2E2_1597 0.58 -0.6 1,870,021 + Pf6N2E2_1597 0.61 -0.8 1,870,254 + Pf6N2E2_1597 0.71 +1.6 1,870,328 - Pf6N2E2_1597 0.74 +0.6 1,870,387 + Pf6N2E2_1597 0.76 -0.2 1,870,388 - Pf6N2E2_1597 0.76 -2.1 1,870,520 - Pf6N2E2_1597 0.81 -1.1 1,870,549 + Pf6N2E2_1597 0.83 -0.2 1,870,624 + Pf6N2E2_1597 0.86 +0.3 1,870,729 + Pf6N2E2_1597 0.90 -0.2 1,870,865 + +0.2 1,870,866 - +0.1 1,871,184 + Pf6N2E2_1598 0.38 +0.2 1,871,319 + Pf6N2E2_1598 0.66 -0.3 1,871,320 - Pf6N2E2_1598 0.66 -0.9 1,871,368 - Pf6N2E2_1598 0.76 +1.4 1,871,404 + Pf6N2E2_1598 0.84 -0.0 1,871,404 + Pf6N2E2_1598 0.84 +1.7 1,871,405 - Pf6N2E2_1598 0.84 +0.4 1,871,405 - Pf6N2E2_1598 0.84 -0.2 1,871,524 - -1.0 1,871,553 + Pf6N2E2_1599 0.10 +1.4 1,871,554 - Pf6N2E2_1599 0.10 +1.4 1,871,554 - Pf6N2E2_1599 0.10 -1.2 1,871,554 - Pf6N2E2_1599 0.10 +1.4 1,871,554 - Pf6N2E2_1599 0.10 -0.3 1,871,554 - Pf6N2E2_1599 0.10 -0.0 1,871,554 - Pf6N2E2_1599 0.10 +0.5 1,871,554 - Pf6N2E2_1599 0.10 -0.6 1,871,557 - Pf6N2E2_1599 0.11 -1.0 1,871,557 - Pf6N2E2_1599 0.11 +0.8 1,871,715 + Pf6N2E2_1599 0.37 +0.6 1,871,781 - Pf6N2E2_1599 0.48 -0.4
Or see this region's nucleotide sequence