Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1408

Experiment: Nitrate reduction, replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1407 and Pf6N2E2_1408 are separated by 51 nucleotides Pf6N2E2_1407 - Nitrous-oxide reductase (EC 1.7.99.6), at 1,647,337 to 1,649,256 _1407 Pf6N2E2_1408 - Nitrous oxide reductase maturation protein NosR, at 1,649,308 to 1,651,458 _1408 Position (kb) 1649 1650 1651 1652Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1648.487 kb on + strand, within Pf6N2E2_1407at 1648.488 kb on - strand, within Pf6N2E2_1407at 1648.517 kb on + strand, within Pf6N2E2_1407at 1648.517 kb on + strand, within Pf6N2E2_1407at 1648.518 kb on - strand, within Pf6N2E2_1407at 1648.635 kb on + strand, within Pf6N2E2_1407at 1648.636 kb on - strand, within Pf6N2E2_1407at 1648.636 kb on - strand, within Pf6N2E2_1407at 1648.636 kb on - strand, within Pf6N2E2_1407at 1648.685 kb on + strand, within Pf6N2E2_1407at 1648.685 kb on + strand, within Pf6N2E2_1407at 1648.685 kb on + strand, within Pf6N2E2_1407at 1648.685 kb on + strand, within Pf6N2E2_1407at 1648.694 kb on + strand, within Pf6N2E2_1407at 1648.782 kb on + strand, within Pf6N2E2_1407at 1648.823 kb on + strand, within Pf6N2E2_1407at 1648.835 kb on + strand, within Pf6N2E2_1407at 1648.835 kb on + strand, within Pf6N2E2_1407at 1648.886 kb on + strand, within Pf6N2E2_1407at 1648.887 kb on - strand, within Pf6N2E2_1407at 1649.008 kb on + strand, within Pf6N2E2_1407at 1649.043 kb on - strand, within Pf6N2E2_1407at 1649.045 kb on + strand, within Pf6N2E2_1407at 1649.045 kb on + strand, within Pf6N2E2_1407at 1649.159 kb on - strandat 1649.445 kb on - strandat 1649.610 kb on - strand, within Pf6N2E2_1408at 1649.792 kb on + strand, within Pf6N2E2_1408at 1649.792 kb on + strand, within Pf6N2E2_1408at 1649.793 kb on - strand, within Pf6N2E2_1408at 1649.793 kb on - strand, within Pf6N2E2_1408at 1649.793 kb on - strand, within Pf6N2E2_1408at 1649.793 kb on - strand, within Pf6N2E2_1408at 1649.793 kb on - strand, within Pf6N2E2_1408at 1649.838 kb on - strand, within Pf6N2E2_1408at 1650.321 kb on - strand, within Pf6N2E2_1408at 1650.346 kb on - strand, within Pf6N2E2_1408at 1650.569 kb on + strand, within Pf6N2E2_1408at 1650.569 kb on + strand, within Pf6N2E2_1408at 1650.570 kb on - strand, within Pf6N2E2_1408at 1650.713 kb on + strand, within Pf6N2E2_1408at 1650.714 kb on - strand, within Pf6N2E2_1408at 1650.714 kb on - strand, within Pf6N2E2_1408at 1650.869 kb on + strand, within Pf6N2E2_1408at 1650.869 kb on + strand, within Pf6N2E2_1408at 1651.157 kb on + strand, within Pf6N2E2_1408at 1651.157 kb on + strand, within Pf6N2E2_1408at 1651.158 kb on - strand, within Pf6N2E2_1408at 1651.158 kb on - strand, within Pf6N2E2_1408at 1651.260 kb on - strandat 1651.260 kb on - strandat 1651.260 kb on - strandat 1651.260 kb on - strandat 1651.260 kb on - strandat 1651.490 kb on + strandat 1651.569 kb on - strandat 1651.587 kb on - strandat 1652.224 kb on + strandat 1652.224 kb on + strandat 1652.375 kb on + strandat 1652.375 kb on + strandat 1652.376 kb on - strandat 1652.376 kb on - strandat 1652.376 kb on - strandat 1652.376 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A
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1,648,487 + Pf6N2E2_1407 0.60 +1.1
1,648,488 - Pf6N2E2_1407 0.60 -0.3
1,648,517 + Pf6N2E2_1407 0.61 -0.2
1,648,517 + Pf6N2E2_1407 0.61 +0.4
1,648,518 - Pf6N2E2_1407 0.62 +0.4
1,648,635 + Pf6N2E2_1407 0.68 +1.1
1,648,636 - Pf6N2E2_1407 0.68 +0.2
1,648,636 - Pf6N2E2_1407 0.68 -0.9
1,648,636 - Pf6N2E2_1407 0.68 +0.4
1,648,685 + Pf6N2E2_1407 0.70 +1.1
1,648,685 + Pf6N2E2_1407 0.70 +1.2
1,648,685 + Pf6N2E2_1407 0.70 -0.4
1,648,685 + Pf6N2E2_1407 0.70 +2.4
1,648,694 + Pf6N2E2_1407 0.71 -1.0
1,648,782 + Pf6N2E2_1407 0.75 +1.1
1,648,823 + Pf6N2E2_1407 0.77 -0.9
1,648,835 + Pf6N2E2_1407 0.78 +0.1
1,648,835 + Pf6N2E2_1407 0.78 +0.0
1,648,886 + Pf6N2E2_1407 0.81 -0.5
1,648,887 - Pf6N2E2_1407 0.81 +0.4
1,649,008 + Pf6N2E2_1407 0.87 -2.0
1,649,043 - Pf6N2E2_1407 0.89 +0.1
1,649,045 + Pf6N2E2_1407 0.89 -0.2
1,649,045 + Pf6N2E2_1407 0.89 +0.7
1,649,159 - +0.1
1,649,445 - +0.5
1,649,610 - Pf6N2E2_1408 0.14 -0.1
1,649,792 + Pf6N2E2_1408 0.23 +0.7
1,649,792 + Pf6N2E2_1408 0.23 -1.2
1,649,793 - Pf6N2E2_1408 0.23 +1.4
1,649,793 - Pf6N2E2_1408 0.23 +0.8
1,649,793 - Pf6N2E2_1408 0.23 -3.2
1,649,793 - Pf6N2E2_1408 0.23 -0.1
1,649,793 - Pf6N2E2_1408 0.23 -0.0
1,649,838 - Pf6N2E2_1408 0.25 -0.3
1,650,321 - Pf6N2E2_1408 0.47 -0.2
1,650,346 - Pf6N2E2_1408 0.48 -1.4
1,650,569 + Pf6N2E2_1408 0.59 +0.5
1,650,569 + Pf6N2E2_1408 0.59 -0.6
1,650,570 - Pf6N2E2_1408 0.59 -0.2
1,650,713 + Pf6N2E2_1408 0.65 -0.2
1,650,714 - Pf6N2E2_1408 0.65 +1.0
1,650,714 - Pf6N2E2_1408 0.65 -0.2
1,650,869 + Pf6N2E2_1408 0.73 -0.4
1,650,869 + Pf6N2E2_1408 0.73 -1.5
1,651,157 + Pf6N2E2_1408 0.86 +1.3
1,651,157 + Pf6N2E2_1408 0.86 -0.8
1,651,158 - Pf6N2E2_1408 0.86 -0.7
1,651,158 - Pf6N2E2_1408 0.86 +0.6
1,651,260 - -0.6
1,651,260 - -0.2
1,651,260 - -0.6
1,651,260 - +0.8
1,651,260 - -0.7
1,651,490 + -1.6
1,651,569 - +1.4
1,651,587 - -0.0
1,652,224 + +0.5
1,652,224 + +1.4
1,652,375 + +0.3
1,652,375 + +0.7
1,652,376 - -0.2
1,652,376 - +0.4
1,652,376 - +0.0
1,652,376 - +0.0

Or see this region's nucleotide sequence