Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1407

Experiment: Nitrate reduction, replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1406 and Pf6N2E2_1407 are separated by 25 nucleotidesPf6N2E2_1407 and Pf6N2E2_1408 are separated by 51 nucleotides Pf6N2E2_1406 - Nitrous oxide reductase maturation protein NosD, at 1,645,998 to 1,647,311 _1406 Pf6N2E2_1407 - Nitrous-oxide reductase (EC 1.7.99.6), at 1,647,337 to 1,649,256 _1407 Pf6N2E2_1408 - Nitrous oxide reductase maturation protein NosR, at 1,649,308 to 1,651,458 _1408 Position (kb) 1647 1648 1649 1650Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1646.441 kb on - strand, within Pf6N2E2_1406at 1646.536 kb on + strand, within Pf6N2E2_1406at 1646.536 kb on + strand, within Pf6N2E2_1406at 1646.536 kb on + strand, within Pf6N2E2_1406at 1646.537 kb on - strand, within Pf6N2E2_1406at 1646.537 kb on - strand, within Pf6N2E2_1406at 1646.545 kb on + strand, within Pf6N2E2_1406at 1646.545 kb on + strand, within Pf6N2E2_1406at 1646.545 kb on + strand, within Pf6N2E2_1406at 1646.545 kb on + strand, within Pf6N2E2_1406at 1646.545 kb on + strand, within Pf6N2E2_1406at 1646.545 kb on + strand, within Pf6N2E2_1406at 1646.640 kb on - strand, within Pf6N2E2_1406at 1646.686 kb on + strand, within Pf6N2E2_1406at 1646.686 kb on + strand, within Pf6N2E2_1406at 1646.686 kb on + strand, within Pf6N2E2_1406at 1646.686 kb on + strand, within Pf6N2E2_1406at 1646.686 kb on + strand, within Pf6N2E2_1406at 1646.686 kb on + strand, within Pf6N2E2_1406at 1646.687 kb on - strand, within Pf6N2E2_1406at 1646.687 kb on - strand, within Pf6N2E2_1406at 1646.755 kb on + strand, within Pf6N2E2_1406at 1646.755 kb on + strand, within Pf6N2E2_1406at 1646.755 kb on + strand, within Pf6N2E2_1406at 1646.756 kb on - strand, within Pf6N2E2_1406at 1646.756 kb on - strand, within Pf6N2E2_1406at 1646.809 kb on - strand, within Pf6N2E2_1406at 1646.815 kb on + strand, within Pf6N2E2_1406at 1647.030 kb on - strand, within Pf6N2E2_1406at 1647.299 kb on + strandat 1647.299 kb on + strandat 1647.300 kb on - strandat 1647.300 kb on - strandat 1647.326 kb on + strandat 1647.404 kb on + strandat 1647.404 kb on + strandat 1647.404 kb on + strandat 1647.406 kb on + strandat 1647.408 kb on - strandat 1647.408 kb on - strandat 1647.590 kb on + strand, within Pf6N2E2_1407at 1647.614 kb on + strand, within Pf6N2E2_1407at 1647.614 kb on + strand, within Pf6N2E2_1407at 1647.857 kb on - strand, within Pf6N2E2_1407at 1647.995 kb on + strand, within Pf6N2E2_1407at 1647.996 kb on - strand, within Pf6N2E2_1407at 1648.079 kb on + strand, within Pf6N2E2_1407at 1648.080 kb on - strand, within Pf6N2E2_1407at 1648.248 kb on - strand, within Pf6N2E2_1407at 1648.302 kb on - strand, within Pf6N2E2_1407at 1648.487 kb on + strand, within Pf6N2E2_1407at 1648.488 kb on - strand, within Pf6N2E2_1407at 1648.517 kb on + strand, within Pf6N2E2_1407at 1648.517 kb on + strand, within Pf6N2E2_1407at 1648.518 kb on - strand, within Pf6N2E2_1407at 1648.635 kb on + strand, within Pf6N2E2_1407at 1648.636 kb on - strand, within Pf6N2E2_1407at 1648.636 kb on - strand, within Pf6N2E2_1407at 1648.636 kb on - strand, within Pf6N2E2_1407at 1648.685 kb on + strand, within Pf6N2E2_1407at 1648.685 kb on + strand, within Pf6N2E2_1407at 1648.685 kb on + strand, within Pf6N2E2_1407at 1648.685 kb on + strand, within Pf6N2E2_1407at 1648.694 kb on + strand, within Pf6N2E2_1407at 1648.782 kb on + strand, within Pf6N2E2_1407at 1648.823 kb on + strand, within Pf6N2E2_1407at 1648.835 kb on + strand, within Pf6N2E2_1407at 1648.835 kb on + strand, within Pf6N2E2_1407at 1648.886 kb on + strand, within Pf6N2E2_1407at 1648.887 kb on - strand, within Pf6N2E2_1407at 1649.008 kb on + strand, within Pf6N2E2_1407at 1649.043 kb on - strand, within Pf6N2E2_1407at 1649.045 kb on + strand, within Pf6N2E2_1407at 1649.045 kb on + strand, within Pf6N2E2_1407at 1649.159 kb on - strandat 1649.445 kb on - strandat 1649.610 kb on - strand, within Pf6N2E2_1408at 1649.792 kb on + strand, within Pf6N2E2_1408at 1649.792 kb on + strand, within Pf6N2E2_1408at 1649.793 kb on - strand, within Pf6N2E2_1408at 1649.793 kb on - strand, within Pf6N2E2_1408at 1649.793 kb on - strand, within Pf6N2E2_1408at 1649.793 kb on - strand, within Pf6N2E2_1408at 1649.793 kb on - strand, within Pf6N2E2_1408at 1649.838 kb on - strand, within Pf6N2E2_1408

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A
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1,646,441 - Pf6N2E2_1406 0.34 -0.4
1,646,536 + Pf6N2E2_1406 0.41 -0.1
1,646,536 + Pf6N2E2_1406 0.41 +0.8
1,646,536 + Pf6N2E2_1406 0.41 +0.1
1,646,537 - Pf6N2E2_1406 0.41 +0.4
1,646,537 - Pf6N2E2_1406 0.41 +0.1
1,646,545 + Pf6N2E2_1406 0.42 -0.3
1,646,545 + Pf6N2E2_1406 0.42 -0.3
1,646,545 + Pf6N2E2_1406 0.42 -0.0
1,646,545 + Pf6N2E2_1406 0.42 +0.6
1,646,545 + Pf6N2E2_1406 0.42 +0.5
1,646,545 + Pf6N2E2_1406 0.42 +0.3
1,646,640 - Pf6N2E2_1406 0.49 +1.8
1,646,686 + Pf6N2E2_1406 0.52 +0.2
1,646,686 + Pf6N2E2_1406 0.52 -0.8
1,646,686 + Pf6N2E2_1406 0.52 +0.5
1,646,686 + Pf6N2E2_1406 0.52 -0.2
1,646,686 + Pf6N2E2_1406 0.52 -1.8
1,646,686 + Pf6N2E2_1406 0.52 -0.1
1,646,687 - Pf6N2E2_1406 0.52 -0.4
1,646,687 - Pf6N2E2_1406 0.52 +1.4
1,646,755 + Pf6N2E2_1406 0.58 -0.2
1,646,755 + Pf6N2E2_1406 0.58 -0.1
1,646,755 + Pf6N2E2_1406 0.58 +0.1
1,646,756 - Pf6N2E2_1406 0.58 -0.6
1,646,756 - Pf6N2E2_1406 0.58 -2.5
1,646,809 - Pf6N2E2_1406 0.62 +0.9
1,646,815 + Pf6N2E2_1406 0.62 +1.4
1,647,030 - Pf6N2E2_1406 0.79 -0.3
1,647,299 + -0.3
1,647,299 + +0.7
1,647,300 - -0.3
1,647,300 - +0.6
1,647,326 + -0.1
1,647,404 + -0.2
1,647,404 + +0.1
1,647,404 + +0.4
1,647,406 + +0.1
1,647,408 - -0.8
1,647,408 - -0.6
1,647,590 + Pf6N2E2_1407 0.13 -0.5
1,647,614 + Pf6N2E2_1407 0.14 +0.4
1,647,614 + Pf6N2E2_1407 0.14 +1.4
1,647,857 - Pf6N2E2_1407 0.27 -0.2
1,647,995 + Pf6N2E2_1407 0.34 -0.0
1,647,996 - Pf6N2E2_1407 0.34 +1.4
1,648,079 + Pf6N2E2_1407 0.39 -0.7
1,648,080 - Pf6N2E2_1407 0.39 +1.2
1,648,248 - Pf6N2E2_1407 0.47 +0.4
1,648,302 - Pf6N2E2_1407 0.50 +1.4
1,648,487 + Pf6N2E2_1407 0.60 +1.1
1,648,488 - Pf6N2E2_1407 0.60 -0.3
1,648,517 + Pf6N2E2_1407 0.61 -0.2
1,648,517 + Pf6N2E2_1407 0.61 +0.4
1,648,518 - Pf6N2E2_1407 0.62 +0.4
1,648,635 + Pf6N2E2_1407 0.68 +1.1
1,648,636 - Pf6N2E2_1407 0.68 +0.2
1,648,636 - Pf6N2E2_1407 0.68 -0.9
1,648,636 - Pf6N2E2_1407 0.68 +0.4
1,648,685 + Pf6N2E2_1407 0.70 +1.1
1,648,685 + Pf6N2E2_1407 0.70 +1.2
1,648,685 + Pf6N2E2_1407 0.70 -0.4
1,648,685 + Pf6N2E2_1407 0.70 +2.4
1,648,694 + Pf6N2E2_1407 0.71 -1.0
1,648,782 + Pf6N2E2_1407 0.75 +1.1
1,648,823 + Pf6N2E2_1407 0.77 -0.9
1,648,835 + Pf6N2E2_1407 0.78 +0.1
1,648,835 + Pf6N2E2_1407 0.78 +0.0
1,648,886 + Pf6N2E2_1407 0.81 -0.5
1,648,887 - Pf6N2E2_1407 0.81 +0.4
1,649,008 + Pf6N2E2_1407 0.87 -2.0
1,649,043 - Pf6N2E2_1407 0.89 +0.1
1,649,045 + Pf6N2E2_1407 0.89 -0.2
1,649,045 + Pf6N2E2_1407 0.89 +0.7
1,649,159 - +0.1
1,649,445 - +0.5
1,649,610 - Pf6N2E2_1408 0.14 -0.1
1,649,792 + Pf6N2E2_1408 0.23 +0.7
1,649,792 + Pf6N2E2_1408 0.23 -1.2
1,649,793 - Pf6N2E2_1408 0.23 +1.4
1,649,793 - Pf6N2E2_1408 0.23 +0.8
1,649,793 - Pf6N2E2_1408 0.23 -3.2
1,649,793 - Pf6N2E2_1408 0.23 -0.1
1,649,793 - Pf6N2E2_1408 0.23 -0.0
1,649,838 - Pf6N2E2_1408 0.25 -0.3

Or see this region's nucleotide sequence