Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1406 and Pf6N2E2_1407 are separated by 25 nucleotides Pf6N2E2_1407 and Pf6N2E2_1408 are separated by 51 nucleotides
Pf6N2E2_1406 - Nitrous oxide reductase maturation protein NosD, at 1,645,998 to 1,647,311
_1406
Pf6N2E2_1407 - Nitrous-oxide reductase (EC 1.7.99.6), at 1,647,337 to 1,649,256
_1407
Pf6N2E2_1408 - Nitrous oxide reductase maturation protein NosR, at 1,649,308 to 1,651,458
_1408
Position (kb)
1647
1648
1649
1650 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1646.441 kb on - strand, within Pf6N2E2_1406 at 1646.536 kb on + strand, within Pf6N2E2_1406 at 1646.536 kb on + strand, within Pf6N2E2_1406 at 1646.536 kb on + strand, within Pf6N2E2_1406 at 1646.537 kb on - strand, within Pf6N2E2_1406 at 1646.537 kb on - strand, within Pf6N2E2_1406 at 1646.545 kb on + strand, within Pf6N2E2_1406 at 1646.545 kb on + strand, within Pf6N2E2_1406 at 1646.545 kb on + strand, within Pf6N2E2_1406 at 1646.545 kb on + strand, within Pf6N2E2_1406 at 1646.545 kb on + strand, within Pf6N2E2_1406 at 1646.545 kb on + strand, within Pf6N2E2_1406 at 1646.640 kb on - strand, within Pf6N2E2_1406 at 1646.686 kb on + strand, within Pf6N2E2_1406 at 1646.686 kb on + strand, within Pf6N2E2_1406 at 1646.686 kb on + strand, within Pf6N2E2_1406 at 1646.686 kb on + strand, within Pf6N2E2_1406 at 1646.686 kb on + strand, within Pf6N2E2_1406 at 1646.686 kb on + strand, within Pf6N2E2_1406 at 1646.687 kb on - strand, within Pf6N2E2_1406 at 1646.687 kb on - strand, within Pf6N2E2_1406 at 1646.755 kb on + strand, within Pf6N2E2_1406 at 1646.755 kb on + strand, within Pf6N2E2_1406 at 1646.755 kb on + strand, within Pf6N2E2_1406 at 1646.756 kb on - strand, within Pf6N2E2_1406 at 1646.756 kb on - strand, within Pf6N2E2_1406 at 1646.809 kb on - strand, within Pf6N2E2_1406 at 1646.815 kb on + strand, within Pf6N2E2_1406 at 1647.030 kb on - strand, within Pf6N2E2_1406 at 1647.299 kb on + strand at 1647.299 kb on + strand at 1647.300 kb on - strand at 1647.300 kb on - strand at 1647.326 kb on + strand at 1647.404 kb on + strand at 1647.404 kb on + strand at 1647.404 kb on + strand at 1647.406 kb on + strand at 1647.408 kb on - strand at 1647.408 kb on - strand at 1647.590 kb on + strand, within Pf6N2E2_1407 at 1647.614 kb on + strand, within Pf6N2E2_1407 at 1647.614 kb on + strand, within Pf6N2E2_1407 at 1647.857 kb on - strand, within Pf6N2E2_1407 at 1647.995 kb on + strand, within Pf6N2E2_1407 at 1647.996 kb on - strand, within Pf6N2E2_1407 at 1648.079 kb on + strand, within Pf6N2E2_1407 at 1648.080 kb on - strand, within Pf6N2E2_1407 at 1648.248 kb on - strand, within Pf6N2E2_1407 at 1648.302 kb on - strand, within Pf6N2E2_1407 at 1648.487 kb on + strand, within Pf6N2E2_1407 at 1648.488 kb on - strand, within Pf6N2E2_1407 at 1648.517 kb on + strand, within Pf6N2E2_1407 at 1648.517 kb on + strand, within Pf6N2E2_1407 at 1648.518 kb on - strand, within Pf6N2E2_1407 at 1648.635 kb on + strand, within Pf6N2E2_1407 at 1648.636 kb on - strand, within Pf6N2E2_1407 at 1648.636 kb on - strand, within Pf6N2E2_1407 at 1648.636 kb on - strand, within Pf6N2E2_1407 at 1648.685 kb on + strand, within Pf6N2E2_1407 at 1648.685 kb on + strand, within Pf6N2E2_1407 at 1648.685 kb on + strand, within Pf6N2E2_1407 at 1648.685 kb on + strand, within Pf6N2E2_1407 at 1648.694 kb on + strand, within Pf6N2E2_1407 at 1648.782 kb on + strand, within Pf6N2E2_1407 at 1648.823 kb on + strand, within Pf6N2E2_1407 at 1648.835 kb on + strand, within Pf6N2E2_1407 at 1648.835 kb on + strand, within Pf6N2E2_1407 at 1648.886 kb on + strand, within Pf6N2E2_1407 at 1648.887 kb on - strand, within Pf6N2E2_1407 at 1649.008 kb on + strand, within Pf6N2E2_1407 at 1649.043 kb on - strand, within Pf6N2E2_1407 at 1649.045 kb on + strand, within Pf6N2E2_1407 at 1649.045 kb on + strand, within Pf6N2E2_1407 at 1649.159 kb on - strand at 1649.445 kb on - strand at 1649.610 kb on - strand, within Pf6N2E2_1408 at 1649.792 kb on + strand, within Pf6N2E2_1408 at 1649.792 kb on + strand, within Pf6N2E2_1408 at 1649.793 kb on - strand, within Pf6N2E2_1408 at 1649.793 kb on - strand, within Pf6N2E2_1408 at 1649.793 kb on - strand, within Pf6N2E2_1408 at 1649.793 kb on - strand, within Pf6N2E2_1408 at 1649.793 kb on - strand, within Pf6N2E2_1408 at 1649.838 kb on - strand, within Pf6N2E2_1408
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 1,646,441 - Pf6N2E2_1406 0.34 -0.4 1,646,536 + Pf6N2E2_1406 0.41 -0.1 1,646,536 + Pf6N2E2_1406 0.41 +0.8 1,646,536 + Pf6N2E2_1406 0.41 +0.1 1,646,537 - Pf6N2E2_1406 0.41 +0.4 1,646,537 - Pf6N2E2_1406 0.41 +0.1 1,646,545 + Pf6N2E2_1406 0.42 -0.3 1,646,545 + Pf6N2E2_1406 0.42 -0.3 1,646,545 + Pf6N2E2_1406 0.42 -0.0 1,646,545 + Pf6N2E2_1406 0.42 +0.6 1,646,545 + Pf6N2E2_1406 0.42 +0.5 1,646,545 + Pf6N2E2_1406 0.42 +0.3 1,646,640 - Pf6N2E2_1406 0.49 +1.8 1,646,686 + Pf6N2E2_1406 0.52 +0.2 1,646,686 + Pf6N2E2_1406 0.52 -0.8 1,646,686 + Pf6N2E2_1406 0.52 +0.5 1,646,686 + Pf6N2E2_1406 0.52 -0.2 1,646,686 + Pf6N2E2_1406 0.52 -1.8 1,646,686 + Pf6N2E2_1406 0.52 -0.1 1,646,687 - Pf6N2E2_1406 0.52 -0.4 1,646,687 - Pf6N2E2_1406 0.52 +1.4 1,646,755 + Pf6N2E2_1406 0.58 -0.2 1,646,755 + Pf6N2E2_1406 0.58 -0.1 1,646,755 + Pf6N2E2_1406 0.58 +0.1 1,646,756 - Pf6N2E2_1406 0.58 -0.6 1,646,756 - Pf6N2E2_1406 0.58 -2.5 1,646,809 - Pf6N2E2_1406 0.62 +0.9 1,646,815 + Pf6N2E2_1406 0.62 +1.4 1,647,030 - Pf6N2E2_1406 0.79 -0.3 1,647,299 + -0.3 1,647,299 + +0.7 1,647,300 - -0.3 1,647,300 - +0.6 1,647,326 + -0.1 1,647,404 + -0.2 1,647,404 + +0.1 1,647,404 + +0.4 1,647,406 + +0.1 1,647,408 - -0.8 1,647,408 - -0.6 1,647,590 + Pf6N2E2_1407 0.13 -0.5 1,647,614 + Pf6N2E2_1407 0.14 +0.4 1,647,614 + Pf6N2E2_1407 0.14 +1.4 1,647,857 - Pf6N2E2_1407 0.27 -0.2 1,647,995 + Pf6N2E2_1407 0.34 -0.0 1,647,996 - Pf6N2E2_1407 0.34 +1.4 1,648,079 + Pf6N2E2_1407 0.39 -0.7 1,648,080 - Pf6N2E2_1407 0.39 +1.2 1,648,248 - Pf6N2E2_1407 0.47 +0.4 1,648,302 - Pf6N2E2_1407 0.50 +1.4 1,648,487 + Pf6N2E2_1407 0.60 +1.1 1,648,488 - Pf6N2E2_1407 0.60 -0.3 1,648,517 + Pf6N2E2_1407 0.61 -0.2 1,648,517 + Pf6N2E2_1407 0.61 +0.4 1,648,518 - Pf6N2E2_1407 0.62 +0.4 1,648,635 + Pf6N2E2_1407 0.68 +1.1 1,648,636 - Pf6N2E2_1407 0.68 +0.2 1,648,636 - Pf6N2E2_1407 0.68 -0.9 1,648,636 - Pf6N2E2_1407 0.68 +0.4 1,648,685 + Pf6N2E2_1407 0.70 +1.1 1,648,685 + Pf6N2E2_1407 0.70 +1.2 1,648,685 + Pf6N2E2_1407 0.70 -0.4 1,648,685 + Pf6N2E2_1407 0.70 +2.4 1,648,694 + Pf6N2E2_1407 0.71 -1.0 1,648,782 + Pf6N2E2_1407 0.75 +1.1 1,648,823 + Pf6N2E2_1407 0.77 -0.9 1,648,835 + Pf6N2E2_1407 0.78 +0.1 1,648,835 + Pf6N2E2_1407 0.78 +0.0 1,648,886 + Pf6N2E2_1407 0.81 -0.5 1,648,887 - Pf6N2E2_1407 0.81 +0.4 1,649,008 + Pf6N2E2_1407 0.87 -2.0 1,649,043 - Pf6N2E2_1407 0.89 +0.1 1,649,045 + Pf6N2E2_1407 0.89 -0.2 1,649,045 + Pf6N2E2_1407 0.89 +0.7 1,649,159 - +0.1 1,649,445 - +0.5 1,649,610 - Pf6N2E2_1408 0.14 -0.1 1,649,792 + Pf6N2E2_1408 0.23 +0.7 1,649,792 + Pf6N2E2_1408 0.23 -1.2 1,649,793 - Pf6N2E2_1408 0.23 +1.4 1,649,793 - Pf6N2E2_1408 0.23 +0.8 1,649,793 - Pf6N2E2_1408 0.23 -3.2 1,649,793 - Pf6N2E2_1408 0.23 -0.1 1,649,793 - Pf6N2E2_1408 0.23 -0.0 1,649,838 - Pf6N2E2_1408 0.25 -0.3
Or see this region's nucleotide sequence