Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1401 and Pf6N2E2_1402 are separated by 15 nucleotides Pf6N2E2_1402 and Pf6N2E2_1403 are separated by 119 nucleotides Pf6N2E2_1403 and Pf6N2E2_1404 are separated by 30 nucleotides Pf6N2E2_1404 and Pf6N2E2_1405 overlap by 4 nucleotides Pf6N2E2_1405 and Pf6N2E2_1406 overlap by 4 nucleotides
Pf6N2E2_1401 - FIG00955631: hypothetical protein, at 1,642,203 to 1,643,366
_1401
Pf6N2E2_1402 - hypothetical protein, at 1,643,382 to 1,643,579
_1402
Pf6N2E2_1403 - Nitrous oxide reductase maturation protein, outer-membrane lipoprotein NosL, at 1,643,699 to 1,644,226
_1403
Pf6N2E2_1404 - Nitrous oxide reductase maturation transmembrane protein NosY, at 1,644,257 to 1,645,087
_1404
Pf6N2E2_1405 - Nitrous oxide reductase maturation protein NosF (ATPase), at 1,645,084 to 1,646,001
_1405
Pf6N2E2_1406 - Nitrous oxide reductase maturation protein NosD, at 1,645,998 to 1,647,311
_1406
Position (kb)
1644
1645
1646 Strain fitness (log2 ratio)
-2
-1
0
1 at 1643.406 kb on - strand, within Pf6N2E2_1402 at 1643.406 kb on - strand, within Pf6N2E2_1402 at 1643.428 kb on + strand, within Pf6N2E2_1402 at 1643.428 kb on + strand, within Pf6N2E2_1402 at 1643.428 kb on + strand, within Pf6N2E2_1402 at 1643.428 kb on + strand, within Pf6N2E2_1402 at 1643.428 kb on + strand, within Pf6N2E2_1402 at 1643.429 kb on - strand, within Pf6N2E2_1402 at 1643.429 kb on - strand, within Pf6N2E2_1402 at 1643.429 kb on - strand, within Pf6N2E2_1402 at 1643.429 kb on - strand, within Pf6N2E2_1402 at 1643.429 kb on - strand, within Pf6N2E2_1402 at 1643.433 kb on + strand, within Pf6N2E2_1402 at 1643.433 kb on + strand, within Pf6N2E2_1402 at 1643.434 kb on - strand, within Pf6N2E2_1402 at 1643.434 kb on - strand, within Pf6N2E2_1402 at 1643.434 kb on - strand, within Pf6N2E2_1402 at 1643.434 kb on - strand, within Pf6N2E2_1402 at 1643.520 kb on + strand, within Pf6N2E2_1402 at 1643.521 kb on - strand, within Pf6N2E2_1402 at 1643.874 kb on + strand, within Pf6N2E2_1403 at 1643.946 kb on + strand, within Pf6N2E2_1403 at 1643.946 kb on + strand, within Pf6N2E2_1403 at 1643.947 kb on - strand, within Pf6N2E2_1403 at 1643.947 kb on - strand, within Pf6N2E2_1403 at 1643.947 kb on - strand, within Pf6N2E2_1403 at 1644.213 kb on + strand at 1644.213 kb on + strand at 1644.214 kb on - strand at 1644.214 kb on - strand at 1644.214 kb on - strand at 1644.314 kb on + strand at 1644.315 kb on - strand at 1644.603 kb on + strand, within Pf6N2E2_1404 at 1644.603 kb on + strand, within Pf6N2E2_1404 at 1644.604 kb on - strand, within Pf6N2E2_1404 at 1644.604 kb on - strand, within Pf6N2E2_1404 at 1644.810 kb on + strand, within Pf6N2E2_1404 at 1644.865 kb on - strand, within Pf6N2E2_1404 at 1645.087 kb on + strand at 1645.121 kb on + strand at 1645.121 kb on + strand at 1645.121 kb on + strand at 1645.121 kb on + strand at 1645.121 kb on + strand at 1645.121 kb on + strand at 1645.121 kb on + strand at 1645.121 kb on + strand at 1645.122 kb on - strand at 1645.122 kb on - strand at 1645.122 kb on - strand at 1645.122 kb on - strand at 1645.122 kb on - strand at 1645.122 kb on - strand at 1645.122 kb on - strand at 1645.206 kb on + strand, within Pf6N2E2_1405 at 1645.207 kb on - strand, within Pf6N2E2_1405 at 1645.499 kb on + strand, within Pf6N2E2_1405 at 1645.499 kb on + strand, within Pf6N2E2_1405 at 1645.500 kb on - strand, within Pf6N2E2_1405 at 1645.616 kb on + strand, within Pf6N2E2_1405 at 1645.617 kb on - strand, within Pf6N2E2_1405 at 1645.617 kb on - strand, within Pf6N2E2_1405 at 1645.745 kb on + strand, within Pf6N2E2_1405 at 1645.745 kb on + strand, within Pf6N2E2_1405 at 1645.770 kb on - strand at 1645.965 kb on - strand at 1645.965 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 1,643,406 - Pf6N2E2_1402 0.12 -0.1 1,643,406 - Pf6N2E2_1402 0.12 +0.3 1,643,428 + Pf6N2E2_1402 0.23 -0.6 1,643,428 + Pf6N2E2_1402 0.23 +0.2 1,643,428 + Pf6N2E2_1402 0.23 -0.5 1,643,428 + Pf6N2E2_1402 0.23 -1.5 1,643,428 + Pf6N2E2_1402 0.23 +1.2 1,643,429 - Pf6N2E2_1402 0.24 +0.3 1,643,429 - Pf6N2E2_1402 0.24 -0.9 1,643,429 - Pf6N2E2_1402 0.24 -0.1 1,643,429 - Pf6N2E2_1402 0.24 -0.3 1,643,429 - Pf6N2E2_1402 0.24 -1.3 1,643,433 + Pf6N2E2_1402 0.26 +0.4 1,643,433 + Pf6N2E2_1402 0.26 -0.2 1,643,434 - Pf6N2E2_1402 0.26 +0.8 1,643,434 - Pf6N2E2_1402 0.26 -0.6 1,643,434 - Pf6N2E2_1402 0.26 -0.5 1,643,434 - Pf6N2E2_1402 0.26 -0.5 1,643,520 + Pf6N2E2_1402 0.70 -1.7 1,643,521 - Pf6N2E2_1402 0.70 +0.6 1,643,874 + Pf6N2E2_1403 0.33 -0.1 1,643,946 + Pf6N2E2_1403 0.47 +1.4 1,643,946 + Pf6N2E2_1403 0.47 +1.4 1,643,947 - Pf6N2E2_1403 0.47 -0.0 1,643,947 - Pf6N2E2_1403 0.47 +0.2 1,643,947 - Pf6N2E2_1403 0.47 +0.4 1,644,213 + +0.8 1,644,213 + -0.2 1,644,214 - +0.2 1,644,214 - -0.1 1,644,214 - +0.2 1,644,314 + +0.4 1,644,315 - -0.6 1,644,603 + Pf6N2E2_1404 0.42 -0.6 1,644,603 + Pf6N2E2_1404 0.42 -0.1 1,644,604 - Pf6N2E2_1404 0.42 -0.6 1,644,604 - Pf6N2E2_1404 0.42 +0.4 1,644,810 + Pf6N2E2_1404 0.67 +0.4 1,644,865 - Pf6N2E2_1404 0.73 +0.9 1,645,087 + -0.9 1,645,121 + -0.3 1,645,121 + -1.1 1,645,121 + -0.2 1,645,121 + -1.0 1,645,121 + -1.1 1,645,121 + +0.5 1,645,121 + +1.4 1,645,121 + +1.4 1,645,122 - -0.7 1,645,122 - -0.3 1,645,122 - +1.4 1,645,122 - +0.8 1,645,122 - -0.3 1,645,122 - -0.5 1,645,122 - -0.0 1,645,206 + Pf6N2E2_1405 0.13 +0.4 1,645,207 - Pf6N2E2_1405 0.13 -1.4 1,645,499 + Pf6N2E2_1405 0.45 +0.7 1,645,499 + Pf6N2E2_1405 0.45 -0.4 1,645,500 - Pf6N2E2_1405 0.45 -0.1 1,645,616 + Pf6N2E2_1405 0.58 -2.0 1,645,617 - Pf6N2E2_1405 0.58 -0.7 1,645,617 - Pf6N2E2_1405 0.58 +0.4 1,645,745 + Pf6N2E2_1405 0.72 -0.1 1,645,745 + Pf6N2E2_1405 0.72 +0.8 1,645,770 - +1.4 1,645,965 - +0.2 1,645,965 - +0.7
Or see this region's nucleotide sequence