Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1294 and Pf6N2E2_1295 are separated by 314 nucleotides Pf6N2E2_1295 and Pf6N2E2_1296 are separated by 31 nucleotides Pf6N2E2_1296 and Pf6N2E2_1297 are separated by 110 nucleotides
Pf6N2E2_1294 - Carbon starvation protein A, at 1,527,572 to 1,529,632
_1294
Pf6N2E2_1295 - Sensor histidine kinase, at 1,529,947 to 1,531,308
_1295
Pf6N2E2_1296 - DNA-binding response regulator, LuxR family, at 1,531,340 to 1,531,972
_1296
Pf6N2E2_1297 - Nucleoside-diphosphate-sugar epimerases, at 1,532,083 to 1,532,952
_1297
Position (kb)
1529
1530
1531
1532 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1528.993 kb on + strand, within Pf6N2E2_1294 at 1529.475 kb on + strand at 1529.496 kb on + strand at 1529.678 kb on - strand at 1529.678 kb on - strand at 1529.678 kb on - strand at 1529.730 kb on + strand at 1529.731 kb on - strand at 1529.731 kb on - strand at 1529.731 kb on - strand at 1529.742 kb on + strand at 1529.743 kb on - strand at 1529.743 kb on - strand at 1529.745 kb on + strand at 1529.746 kb on - strand at 1529.748 kb on + strand at 1529.748 kb on + strand at 1529.748 kb on + strand at 1529.748 kb on + strand at 1529.748 kb on + strand at 1529.749 kb on - strand at 1529.752 kb on + strand at 1529.752 kb on + strand at 1529.752 kb on + strand at 1529.752 kb on + strand at 1529.753 kb on - strand at 1529.753 kb on - strand at 1529.753 kb on - strand at 1529.753 kb on - strand at 1529.753 kb on - strand at 1529.803 kb on + strand at 1529.815 kb on + strand at 1529.816 kb on - strand at 1529.876 kb on + strand at 1529.876 kb on + strand at 1529.900 kb on + strand at 1529.901 kb on - strand at 1529.901 kb on - strand at 1529.901 kb on - strand at 1529.942 kb on + strand at 1529.942 kb on + strand at 1529.942 kb on + strand at 1529.942 kb on + strand at 1529.942 kb on + strand at 1529.943 kb on - strand at 1529.943 kb on - strand at 1529.943 kb on - strand at 1529.943 kb on - strand at 1529.943 kb on - strand at 1530.112 kb on + strand, within Pf6N2E2_1295 at 1530.112 kb on + strand, within Pf6N2E2_1295 at 1530.113 kb on - strand, within Pf6N2E2_1295 at 1530.222 kb on - strand at 1530.242 kb on - strand, within Pf6N2E2_1295 at 1530.299 kb on - strand, within Pf6N2E2_1295 at 1530.309 kb on + strand, within Pf6N2E2_1295 at 1530.385 kb on + strand, within Pf6N2E2_1295 at 1530.433 kb on + strand, within Pf6N2E2_1295 at 1530.434 kb on - strand, within Pf6N2E2_1295 at 1530.434 kb on - strand, within Pf6N2E2_1295 at 1530.593 kb on + strand, within Pf6N2E2_1295 at 1530.593 kb on + strand, within Pf6N2E2_1295 at 1530.670 kb on - strand, within Pf6N2E2_1295 at 1531.164 kb on - strand, within Pf6N2E2_1295 at 1531.605 kb on - strand, within Pf6N2E2_1296 at 1531.605 kb on - strand, within Pf6N2E2_1296 at 1531.613 kb on + strand, within Pf6N2E2_1296 at 1531.619 kb on + strand, within Pf6N2E2_1296 at 1531.620 kb on - strand, within Pf6N2E2_1296 at 1531.659 kb on + strand, within Pf6N2E2_1296 at 1531.660 kb on - strand, within Pf6N2E2_1296 at 1531.727 kb on + strand, within Pf6N2E2_1296 at 1531.727 kb on + strand, within Pf6N2E2_1296 at 1531.727 kb on + strand, within Pf6N2E2_1296 at 1531.727 kb on + strand, within Pf6N2E2_1296 at 1531.728 kb on - strand, within Pf6N2E2_1296 at 1531.941 kb on - strand at 1532.025 kb on + strand at 1532.096 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 1,528,993 + Pf6N2E2_1294 0.69 -1.4 1,529,475 + -1.4 1,529,496 + -2.6 1,529,678 - -1.1 1,529,678 - -0.1 1,529,678 - -1.7 1,529,730 + -2.7 1,529,731 - -1.0 1,529,731 - +0.9 1,529,731 - +1.8 1,529,742 + -1.6 1,529,743 - +0.9 1,529,743 - -0.8 1,529,745 + +0.4 1,529,746 - -0.9 1,529,748 + -1.6 1,529,748 + -1.4 1,529,748 + -1.3 1,529,748 + -2.5 1,529,748 + -3.4 1,529,749 - -0.6 1,529,752 + -2.9 1,529,752 + -0.9 1,529,752 + -1.9 1,529,752 + -2.2 1,529,753 - +0.9 1,529,753 - +0.4 1,529,753 - -0.1 1,529,753 - +0.9 1,529,753 - +0.6 1,529,803 + -1.1 1,529,815 + +0.3 1,529,816 - +0.4 1,529,876 + -0.5 1,529,876 + +0.8 1,529,900 + -0.9 1,529,901 - +1.9 1,529,901 - +0.9 1,529,901 - -0.1 1,529,942 + +1.4 1,529,942 + +0.2 1,529,942 + +0.9 1,529,942 + -0.9 1,529,942 + +1.4 1,529,943 - -0.1 1,529,943 - +1.4 1,529,943 - +0.4 1,529,943 - +1.6 1,529,943 - -0.3 1,530,112 + Pf6N2E2_1295 0.12 -1.6 1,530,112 + Pf6N2E2_1295 0.12 -1.4 1,530,113 - Pf6N2E2_1295 0.12 +1.4 1,530,222 - -0.6 1,530,242 - Pf6N2E2_1295 0.22 +1.4 1,530,299 - Pf6N2E2_1295 0.26 -2.0 1,530,309 + Pf6N2E2_1295 0.27 -1.5 1,530,385 + Pf6N2E2_1295 0.32 -1.6 1,530,433 + Pf6N2E2_1295 0.36 -1.4 1,530,434 - Pf6N2E2_1295 0.36 -3.7 1,530,434 - Pf6N2E2_1295 0.36 -3.4 1,530,593 + Pf6N2E2_1295 0.47 -0.6 1,530,593 + Pf6N2E2_1295 0.47 +0.0 1,530,670 - Pf6N2E2_1295 0.53 -1.6 1,531,164 - Pf6N2E2_1295 0.89 -0.6 1,531,605 - Pf6N2E2_1296 0.42 -1.8 1,531,605 - Pf6N2E2_1296 0.42 -3.1 1,531,613 + Pf6N2E2_1296 0.43 +1.4 1,531,619 + Pf6N2E2_1296 0.44 -2.6 1,531,620 - Pf6N2E2_1296 0.44 -3.7 1,531,659 + Pf6N2E2_1296 0.50 +0.4 1,531,660 - Pf6N2E2_1296 0.51 -1.2 1,531,727 + Pf6N2E2_1296 0.61 -2.9 1,531,727 + Pf6N2E2_1296 0.61 -3.4 1,531,727 + Pf6N2E2_1296 0.61 -2.3 1,531,727 + Pf6N2E2_1296 0.61 -2.2 1,531,728 - Pf6N2E2_1296 0.61 -1.3 1,531,941 - -2.3 1,532,025 + -0.2 1,532,096 + -0.1
Or see this region's nucleotide sequence