Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1104 and Pf6N2E2_1105 are separated by 17 nucleotides Pf6N2E2_1105 and Pf6N2E2_1106 are separated by 141 nucleotides
Pf6N2E2_1104 - Gluconate dehydratase (EC 4.2.1.39), at 1,290,551 to 1,291,750
_1104
Pf6N2E2_1105 - Nitrate/nitrite transporter, at 1,291,768 to 1,293,063
_1105
Pf6N2E2_1106 - Chromosome initiation inhibitor, at 1,293,205 to 1,294,134
_1106
Position (kb)
1291
1292
1293
1294 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1290.777 kb on + strand, within Pf6N2E2_1104 at 1290.777 kb on + strand, within Pf6N2E2_1104 at 1290.783 kb on + strand, within Pf6N2E2_1104 at 1290.783 kb on + strand, within Pf6N2E2_1104 at 1290.784 kb on - strand, within Pf6N2E2_1104 at 1290.807 kb on - strand, within Pf6N2E2_1104 at 1290.977 kb on + strand, within Pf6N2E2_1104 at 1291.083 kb on + strand, within Pf6N2E2_1104 at 1291.084 kb on - strand, within Pf6N2E2_1104 at 1291.410 kb on + strand, within Pf6N2E2_1104 at 1291.410 kb on + strand, within Pf6N2E2_1104 at 1291.410 kb on + strand, within Pf6N2E2_1104 at 1291.410 kb on + strand, within Pf6N2E2_1104 at 1291.425 kb on + strand, within Pf6N2E2_1104 at 1291.425 kb on + strand, within Pf6N2E2_1104 at 1291.425 kb on + strand, within Pf6N2E2_1104 at 1291.425 kb on + strand, within Pf6N2E2_1104 at 1291.426 kb on - strand, within Pf6N2E2_1104 at 1291.503 kb on + strand, within Pf6N2E2_1104 at 1291.629 kb on + strand, within Pf6N2E2_1104 at 1291.744 kb on + strand at 1291.744 kb on + strand at 1291.750 kb on + strand at 1291.750 kb on + strand at 1291.750 kb on + strand at 1291.751 kb on - strand at 1291.751 kb on - strand at 1291.751 kb on - strand at 1291.751 kb on - strand at 1291.751 kb on - strand at 1291.751 kb on - strand at 1291.751 kb on - strand at 1291.769 kb on + strand at 1291.770 kb on - strand at 1291.862 kb on + strand at 1291.863 kb on - strand at 1292.184 kb on - strand, within Pf6N2E2_1105 at 1292.283 kb on - strand, within Pf6N2E2_1105 at 1292.297 kb on + strand, within Pf6N2E2_1105 at 1292.322 kb on - strand, within Pf6N2E2_1105 at 1292.336 kb on + strand, within Pf6N2E2_1105 at 1292.336 kb on + strand, within Pf6N2E2_1105 at 1292.336 kb on + strand, within Pf6N2E2_1105 at 1292.336 kb on + strand, within Pf6N2E2_1105 at 1292.337 kb on - strand, within Pf6N2E2_1105 at 1292.480 kb on + strand, within Pf6N2E2_1105 at 1292.486 kb on + strand, within Pf6N2E2_1105 at 1292.631 kb on - strand, within Pf6N2E2_1105 at 1292.675 kb on + strand, within Pf6N2E2_1105 at 1292.675 kb on + strand, within Pf6N2E2_1105 at 1292.675 kb on + strand, within Pf6N2E2_1105 at 1292.675 kb on + strand, within Pf6N2E2_1105 at 1292.675 kb on + strand, within Pf6N2E2_1105 at 1292.675 kb on + strand, within Pf6N2E2_1105 at 1292.676 kb on - strand, within Pf6N2E2_1105 at 1292.676 kb on - strand, within Pf6N2E2_1105 at 1292.676 kb on - strand, within Pf6N2E2_1105 at 1292.684 kb on + strand, within Pf6N2E2_1105 at 1292.684 kb on + strand, within Pf6N2E2_1105 at 1292.685 kb on - strand, within Pf6N2E2_1105 at 1292.993 kb on + strand at 1292.993 kb on + strand at 1292.993 kb on + strand at 1292.993 kb on + strand at 1292.993 kb on + strand at 1292.993 kb on + strand at 1292.994 kb on - strand at 1292.994 kb on - strand at 1292.994 kb on - strand at 1293.001 kb on + strand at 1293.040 kb on + strand at 1293.078 kb on - strand at 1293.084 kb on + strand at 1293.084 kb on + strand at 1293.084 kb on + strand at 1293.085 kb on - strand at 1293.085 kb on - strand at 1293.094 kb on + strand at 1293.108 kb on + strand at 1293.203 kb on + strand at 1293.203 kb on + strand at 1293.203 kb on + strand at 1293.204 kb on - strand at 1293.437 kb on + strand, within Pf6N2E2_1106 at 1293.437 kb on + strand, within Pf6N2E2_1106 at 1293.496 kb on + strand, within Pf6N2E2_1106 at 1293.698 kb on - strand, within Pf6N2E2_1106 at 1293.913 kb on + strand, within Pf6N2E2_1106 at 1293.914 kb on - strand, within Pf6N2E2_1106 at 1293.914 kb on - strand, within Pf6N2E2_1106 at 1294.028 kb on + strand, within Pf6N2E2_1106 at 1294.028 kb on + strand, within Pf6N2E2_1106 at 1294.029 kb on - strand, within Pf6N2E2_1106 at 1294.029 kb on - strand, within Pf6N2E2_1106
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 1,290,777 + Pf6N2E2_1104 0.19 +0.4 1,290,777 + Pf6N2E2_1104 0.19 -1.1 1,290,783 + Pf6N2E2_1104 0.19 +0.4 1,290,783 + Pf6N2E2_1104 0.19 +1.4 1,290,784 - Pf6N2E2_1104 0.19 -0.1 1,290,807 - Pf6N2E2_1104 0.21 +0.5 1,290,977 + Pf6N2E2_1104 0.35 -0.1 1,291,083 + Pf6N2E2_1104 0.44 +0.0 1,291,084 - Pf6N2E2_1104 0.44 -1.6 1,291,410 + Pf6N2E2_1104 0.72 -0.1 1,291,410 + Pf6N2E2_1104 0.72 -0.1 1,291,410 + Pf6N2E2_1104 0.72 +1.4 1,291,410 + Pf6N2E2_1104 0.72 +0.1 1,291,425 + Pf6N2E2_1104 0.73 +3.4 1,291,425 + Pf6N2E2_1104 0.73 +0.5 1,291,425 + Pf6N2E2_1104 0.73 +0.9 1,291,425 + Pf6N2E2_1104 0.73 +1.4 1,291,426 - Pf6N2E2_1104 0.73 +0.4 1,291,503 + Pf6N2E2_1104 0.79 -0.5 1,291,629 + Pf6N2E2_1104 0.90 -0.1 1,291,744 + +1.4 1,291,744 + +1.0 1,291,750 + +0.7 1,291,750 + -0.1 1,291,750 + +0.6 1,291,751 - +1.0 1,291,751 - +0.0 1,291,751 - +0.6 1,291,751 - +1.4 1,291,751 - -0.6 1,291,751 - -0.4 1,291,751 - -0.2 1,291,769 + -0.1 1,291,770 - +0.4 1,291,862 + -0.6 1,291,863 - -0.6 1,292,184 - Pf6N2E2_1105 0.32 -0.4 1,292,283 - Pf6N2E2_1105 0.40 -0.8 1,292,297 + Pf6N2E2_1105 0.41 +0.2 1,292,322 - Pf6N2E2_1105 0.43 -0.9 1,292,336 + Pf6N2E2_1105 0.44 -0.1 1,292,336 + Pf6N2E2_1105 0.44 -2.2 1,292,336 + Pf6N2E2_1105 0.44 -0.2 1,292,336 + Pf6N2E2_1105 0.44 -0.1 1,292,337 - Pf6N2E2_1105 0.44 -0.9 1,292,480 + Pf6N2E2_1105 0.55 +0.4 1,292,486 + Pf6N2E2_1105 0.55 +0.5 1,292,631 - Pf6N2E2_1105 0.67 +0.7 1,292,675 + Pf6N2E2_1105 0.70 +0.3 1,292,675 + Pf6N2E2_1105 0.70 -0.4 1,292,675 + Pf6N2E2_1105 0.70 +0.0 1,292,675 + Pf6N2E2_1105 0.70 +0.6 1,292,675 + Pf6N2E2_1105 0.70 +1.4 1,292,675 + Pf6N2E2_1105 0.70 +0.8 1,292,676 - Pf6N2E2_1105 0.70 +0.1 1,292,676 - Pf6N2E2_1105 0.70 +0.0 1,292,676 - Pf6N2E2_1105 0.70 +0.6 1,292,684 + Pf6N2E2_1105 0.71 +1.4 1,292,684 + Pf6N2E2_1105 0.71 -0.8 1,292,685 - Pf6N2E2_1105 0.71 -0.7 1,292,993 + -0.4 1,292,993 + +0.3 1,292,993 + -0.2 1,292,993 + +2.0 1,292,993 + +1.4 1,292,993 + +1.4 1,292,994 - -0.2 1,292,994 - +0.4 1,292,994 - +0.4 1,293,001 + +0.9 1,293,040 + +0.9 1,293,078 - -0.9 1,293,084 + +0.0 1,293,084 + +1.4 1,293,084 + +0.1 1,293,085 - -0.8 1,293,085 - +0.3 1,293,094 + +0.1 1,293,108 + -0.6 1,293,203 + +1.7 1,293,203 + -0.4 1,293,203 + -0.4 1,293,204 - +0.3 1,293,437 + Pf6N2E2_1106 0.25 -0.5 1,293,437 + Pf6N2E2_1106 0.25 +0.4 1,293,496 + Pf6N2E2_1106 0.31 +0.4 1,293,698 - Pf6N2E2_1106 0.53 -1.0 1,293,913 + Pf6N2E2_1106 0.76 -0.6 1,293,914 - Pf6N2E2_1106 0.76 -0.4 1,293,914 - Pf6N2E2_1106 0.76 +1.8 1,294,028 + Pf6N2E2_1106 0.88 -0.0 1,294,028 + Pf6N2E2_1106 0.88 +2.0 1,294,029 - Pf6N2E2_1106 0.89 -0.7 1,294,029 - Pf6N2E2_1106 0.89 -0.6
Or see this region's nucleotide sequence