Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1013 and Pf6N2E2_1014 are separated by 436 nucleotides Pf6N2E2_1014 and Pf6N2E2_1015 are separated by 552 nucleotides
Pf6N2E2_1013 - Enoyl-CoA hydratase (EC 4.2.1.17), at 1,182,929 to 1,183,753
_1013
Pf6N2E2_1014 - Nitrate/nitrite transporter, at 1,184,190 to 1,184,381
_1014
Pf6N2E2_1015 - Inositol transport system sugar-binding protein, at 1,184,934 to 1,185,857
_1015
Position (kb)
1184
1185 Strain fitness (log2 ratio)
-2
-1
0
1 at 1183.245 kb on + strand, within Pf6N2E2_1013 at 1183.245 kb on + strand, within Pf6N2E2_1013 at 1183.297 kb on - strand, within Pf6N2E2_1013 at 1183.439 kb on + strand, within Pf6N2E2_1013 at 1183.625 kb on + strand, within Pf6N2E2_1013 at 1183.625 kb on + strand, within Pf6N2E2_1013 at 1183.625 kb on + strand, within Pf6N2E2_1013 at 1183.626 kb on - strand, within Pf6N2E2_1013 at 1183.634 kb on + strand, within Pf6N2E2_1013 at 1183.635 kb on - strand, within Pf6N2E2_1013 at 1183.752 kb on - strand at 1183.756 kb on - strand at 1183.756 kb on - strand at 1183.756 kb on - strand at 1183.796 kb on + strand at 1183.833 kb on + strand at 1183.837 kb on + strand at 1183.838 kb on - strand at 1183.868 kb on + strand at 1183.908 kb on - strand at 1183.996 kb on + strand at 1183.996 kb on + strand at 1183.997 kb on - strand at 1184.195 kb on + strand at 1184.254 kb on - strand, within Pf6N2E2_1014 at 1184.254 kb on - strand, within Pf6N2E2_1014 at 1184.280 kb on - strand, within Pf6N2E2_1014 at 1184.600 kb on - strand at 1184.600 kb on - strand at 1184.611 kb on + strand at 1184.622 kb on + strand at 1184.700 kb on - strand at 1184.709 kb on + strand at 1184.799 kb on + strand at 1184.799 kb on + strand at 1184.799 kb on + strand at 1184.800 kb on - strand at 1184.801 kb on + strand at 1184.801 kb on + strand at 1184.801 kb on + strand at 1184.801 kb on + strand at 1184.802 kb on - strand at 1184.816 kb on - strand at 1184.845 kb on - strand at 1184.845 kb on - strand at 1184.847 kb on + strand at 1184.847 kb on + strand at 1184.847 kb on + strand at 1184.847 kb on + strand at 1184.847 kb on + strand at 1184.848 kb on - strand at 1184.848 kb on - strand at 1184.848 kb on - strand at 1184.866 kb on - strand at 1184.956 kb on + strand at 1184.957 kb on - strand at 1184.980 kb on + strand at 1185.260 kb on + strand, within Pf6N2E2_1015 at 1185.260 kb on + strand, within Pf6N2E2_1015 at 1185.261 kb on - strand, within Pf6N2E2_1015 at 1185.343 kb on - strand, within Pf6N2E2_1015
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 1,183,245 + Pf6N2E2_1013 0.38 -0.8 1,183,245 + Pf6N2E2_1013 0.38 -0.6 1,183,297 - Pf6N2E2_1013 0.45 -1.0 1,183,439 + Pf6N2E2_1013 0.62 +0.3 1,183,625 + Pf6N2E2_1013 0.84 +0.2 1,183,625 + Pf6N2E2_1013 0.84 +1.5 1,183,625 + Pf6N2E2_1013 0.84 -2.7 1,183,626 - Pf6N2E2_1013 0.84 +0.3 1,183,634 + Pf6N2E2_1013 0.85 +0.2 1,183,635 - Pf6N2E2_1013 0.86 -0.0 1,183,752 - -0.4 1,183,756 - +1.3 1,183,756 - +0.1 1,183,756 - +0.5 1,183,796 + -0.5 1,183,833 + +0.9 1,183,837 + -0.1 1,183,838 - -0.8 1,183,868 + -0.4 1,183,908 - +0.4 1,183,996 + +1.5 1,183,996 + -0.0 1,183,997 - -1.1 1,184,195 + -0.8 1,184,254 - Pf6N2E2_1014 0.33 +1.5 1,184,254 - Pf6N2E2_1014 0.33 -0.3 1,184,280 - Pf6N2E2_1014 0.47 +0.7 1,184,600 - +1.7 1,184,600 - +1.5 1,184,611 + -0.2 1,184,622 + +0.6 1,184,700 - -0.4 1,184,709 + +0.7 1,184,799 + +0.1 1,184,799 + +0.3 1,184,799 + -0.5 1,184,800 - +1.5 1,184,801 + +0.4 1,184,801 + -0.8 1,184,801 + -0.2 1,184,801 + -0.8 1,184,802 - +1.5 1,184,816 - +0.5 1,184,845 - -0.3 1,184,845 - -0.7 1,184,847 + -2.4 1,184,847 + -0.6 1,184,847 + +0.3 1,184,847 + +0.5 1,184,847 + -0.2 1,184,848 - +0.5 1,184,848 - -1.3 1,184,848 - -0.3 1,184,866 - -1.1 1,184,956 + -0.5 1,184,957 - -1.0 1,184,980 + -0.6 1,185,260 + Pf6N2E2_1015 0.35 -0.7 1,185,260 + Pf6N2E2_1015 0.35 -0.1 1,185,261 - Pf6N2E2_1015 0.35 +1.7 1,185,343 - Pf6N2E2_1015 0.44 +0.1
Or see this region's nucleotide sequence