Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_835 and Pf6N2E2_836 are separated by 231 nucleotides Pf6N2E2_836 and Pf6N2E2_837 are separated by 7 nucleotides
Pf6N2E2_835 - 3-oxoacyl-[acyl-carrier-protein] synthase, KASII (EC 2.3.1.179), at 976,137 to 977,429
_835
Pf6N2E2_836 - ABC-type protease/lipase transport system, ATPase and permease components, at 977,661 to 979,376
_836
Pf6N2E2_837 - HlyD family secretion protein, at 979,384 to 980,706
_837
Position (kb) 977
978
979
980 Strain fitness (log2 ratio) -2
-1
0
1
2 at 976.733 kb on + strand, within Pf6N2E2_835 at 976.733 kb on + strand, within Pf6N2E2_835 at 976.733 kb on + strand, within Pf6N2E2_835 at 976.733 kb on + strand, within Pf6N2E2_835 at 976.733 kb on + strand, within Pf6N2E2_835 at 976.734 kb on - strand, within Pf6N2E2_835 at 976.734 kb on - strand, within Pf6N2E2_835 at 977.078 kb on + strand, within Pf6N2E2_835 at 977.079 kb on - strand, within Pf6N2E2_835 at 977.171 kb on + strand, within Pf6N2E2_835 at 977.171 kb on + strand, within Pf6N2E2_835 at 977.171 kb on + strand, within Pf6N2E2_835 at 977.172 kb on - strand, within Pf6N2E2_835 at 977.181 kb on - strand, within Pf6N2E2_835 at 977.477 kb on + strand at 977.542 kb on + strand at 977.542 kb on + strand at 977.542 kb on + strand at 977.587 kb on - strand at 977.714 kb on + strand at 977.778 kb on + strand at 977.778 kb on + strand at 977.779 kb on - strand at 977.779 kb on - strand at 977.779 kb on - strand at 977.779 kb on - strand at 977.794 kb on - strand at 977.982 kb on - strand, within Pf6N2E2_836 at 977.982 kb on - strand, within Pf6N2E2_836 at 978.126 kb on + strand, within Pf6N2E2_836 at 978.127 kb on - strand, within Pf6N2E2_836 at 978.442 kb on - strand, within Pf6N2E2_836 at 978.442 kb on - strand, within Pf6N2E2_836 at 978.442 kb on - strand, within Pf6N2E2_836 at 978.571 kb on - strand, within Pf6N2E2_836 at 978.571 kb on - strand, within Pf6N2E2_836 at 978.823 kb on + strand, within Pf6N2E2_836 at 978.876 kb on + strand, within Pf6N2E2_836 at 978.877 kb on - strand, within Pf6N2E2_836 at 979.026 kb on + strand, within Pf6N2E2_836 at 979.104 kb on + strand, within Pf6N2E2_836 at 979.104 kb on + strand, within Pf6N2E2_836 at 979.375 kb on - strand at 979.966 kb on + strand, within Pf6N2E2_837 at 979.967 kb on - strand, within Pf6N2E2_837 at 979.967 kb on - strand, within Pf6N2E2_837 at 979.979 kb on + strand, within Pf6N2E2_837 at 980.012 kb on - strand, within Pf6N2E2_837 at 980.012 kb on - strand, within Pf6N2E2_837 at 980.072 kb on + strand, within Pf6N2E2_837 at 980.203 kb on + strand, within Pf6N2E2_837 at 980.203 kb on + strand, within Pf6N2E2_837 at 980.203 kb on + strand, within Pf6N2E2_837 at 980.203 kb on + strand, within Pf6N2E2_837 at 980.203 kb on + strand, within Pf6N2E2_837 at 980.204 kb on - strand, within Pf6N2E2_837 at 980.204 kb on - strand, within Pf6N2E2_837 at 980.257 kb on - strand, within Pf6N2E2_837
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C remove 976,733 + Pf6N2E2_835 0.46 +0.1 976,733 + Pf6N2E2_835 0.46 -1.0 976,733 + Pf6N2E2_835 0.46 -0.0 976,733 + Pf6N2E2_835 0.46 +0.7 976,733 + Pf6N2E2_835 0.46 -1.3 976,734 - Pf6N2E2_835 0.46 -0.6 976,734 - Pf6N2E2_835 0.46 +0.2 977,078 + Pf6N2E2_835 0.73 -0.6 977,079 - Pf6N2E2_835 0.73 -0.7 977,171 + Pf6N2E2_835 0.80 +0.5 977,171 + Pf6N2E2_835 0.80 +1.0 977,171 + Pf6N2E2_835 0.80 -2.4 977,172 - Pf6N2E2_835 0.80 +1.3 977,181 - Pf6N2E2_835 0.81 +0.2 977,477 + -0.5 977,542 + -0.4 977,542 + -0.3 977,542 + -0.7 977,587 - +0.1 977,714 + +1.5 977,778 + +1.5 977,778 + +1.5 977,779 - +1.5 977,779 - +1.5 977,779 - -0.3 977,779 - -0.9 977,794 - +0.6 977,982 - Pf6N2E2_836 0.19 -0.0 977,982 - Pf6N2E2_836 0.19 +0.7 978,126 + Pf6N2E2_836 0.27 -0.1 978,127 - Pf6N2E2_836 0.27 +1.1 978,442 - Pf6N2E2_836 0.46 +1.4 978,442 - Pf6N2E2_836 0.46 +0.9 978,442 - Pf6N2E2_836 0.46 +0.9 978,571 - Pf6N2E2_836 0.53 +0.7 978,571 - Pf6N2E2_836 0.53 -0.2 978,823 + Pf6N2E2_836 0.68 +1.0 978,876 + Pf6N2E2_836 0.71 +0.7 978,877 - Pf6N2E2_836 0.71 +0.0 979,026 + Pf6N2E2_836 0.80 +0.5 979,104 + Pf6N2E2_836 0.84 +0.1 979,104 + Pf6N2E2_836 0.84 +0.5 979,375 - +0.5 979,966 + Pf6N2E2_837 0.44 -0.2 979,967 - Pf6N2E2_837 0.44 +2.1 979,967 - Pf6N2E2_837 0.44 +1.7 979,979 + Pf6N2E2_837 0.45 +1.5 980,012 - Pf6N2E2_837 0.47 -0.5 980,012 - Pf6N2E2_837 0.47 +0.2 980,072 + Pf6N2E2_837 0.52 -0.1 980,203 + Pf6N2E2_837 0.62 -0.6 980,203 + Pf6N2E2_837 0.62 +0.0 980,203 + Pf6N2E2_837 0.62 -0.3 980,203 + Pf6N2E2_837 0.62 +1.5 980,203 + Pf6N2E2_837 0.62 +0.4 980,204 - Pf6N2E2_837 0.62 +1.5 980,204 - Pf6N2E2_837 0.62 -0.1 980,257 - Pf6N2E2_837 0.66 -0.5
Or see this region's nucleotide sequence