Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_476 and Pf6N2E2_477 are separated by 175 nucleotides Pf6N2E2_477 and Pf6N2E2_478 are separated by 283 nucleotides
Pf6N2E2_476 - Permease of the drug/metabolite transporter (DMT) superfamily, at 541,092 to 542,021
_476
Pf6N2E2_477 - Branched-chain amino acid aminotransferase (EC 2.6.1.42), at 542,197 to 543,216
_477
Pf6N2E2_478 - Dihydrolipoyl dehydrogenase (EC 1.8.1.4) <small>(from data)</small>, at 543,500 to 544,885
_478
Position (kb) 542
543
544 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 541.373 kb on + strand, within Pf6N2E2_476 at 541.373 kb on + strand, within Pf6N2E2_476 at 541.405 kb on - strand, within Pf6N2E2_476 at 541.615 kb on + strand, within Pf6N2E2_476 at 541.616 kb on - strand, within Pf6N2E2_476 at 541.919 kb on + strand, within Pf6N2E2_476 at 542.019 kb on + strand at 542.264 kb on + strand at 542.327 kb on + strand, within Pf6N2E2_477 at 542.327 kb on + strand, within Pf6N2E2_477 at 542.328 kb on - strand, within Pf6N2E2_477 at 542.328 kb on - strand, within Pf6N2E2_477 at 542.328 kb on - strand, within Pf6N2E2_477 at 542.652 kb on - strand, within Pf6N2E2_477 at 542.747 kb on + strand, within Pf6N2E2_477 at 542.843 kb on + strand, within Pf6N2E2_477 at 543.173 kb on + strand at 543.174 kb on - strand at 543.174 kb on - strand at 543.226 kb on + strand at 543.226 kb on + strand at 543.227 kb on - strand at 543.227 kb on - strand at 543.288 kb on + strand at 543.288 kb on + strand at 543.288 kb on + strand at 543.288 kb on + strand at 543.288 kb on + strand at 543.288 kb on + strand at 543.289 kb on - strand at 543.289 kb on - strand at 543.289 kb on - strand at 543.289 kb on - strand at 543.289 kb on - strand at 543.289 kb on - strand at 543.289 kb on - strand at 543.289 kb on - strand at 543.289 kb on - strand at 543.501 kb on + strand at 544.195 kb on - strand, within Pf6N2E2_478 at 544.195 kb on - strand, within Pf6N2E2_478
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C remove 541,373 + Pf6N2E2_476 0.30 +2.1 541,373 + Pf6N2E2_476 0.30 -0.5 541,405 - Pf6N2E2_476 0.34 -1.1 541,615 + Pf6N2E2_476 0.56 -0.1 541,616 - Pf6N2E2_476 0.56 +0.4 541,919 + Pf6N2E2_476 0.89 +0.0 542,019 + -0.1 542,264 + -1.9 542,327 + Pf6N2E2_477 0.13 -3.4 542,327 + Pf6N2E2_477 0.13 -2.2 542,328 - Pf6N2E2_477 0.13 -4.0 542,328 - Pf6N2E2_477 0.13 -4.1 542,328 - Pf6N2E2_477 0.13 -2.6 542,652 - Pf6N2E2_477 0.45 -0.2 542,747 + Pf6N2E2_477 0.54 -2.3 542,843 + Pf6N2E2_477 0.63 -3.4 543,173 + -2.8 543,174 - -1.4 543,174 - -2.7 543,226 + -2.2 543,226 + -1.6 543,227 - -0.1 543,227 - +1.4 543,288 + +0.1 543,288 + -1.6 543,288 + -2.4 543,288 + -1.4 543,288 + -2.0 543,288 + -2.0 543,289 - +0.6 543,289 - +1.4 543,289 - +0.1 543,289 - +2.4 543,289 - -0.3 543,289 - -0.1 543,289 - +0.2 543,289 - -0.1 543,289 - -1.0 543,501 + +0.3 544,195 - Pf6N2E2_478 0.50 -0.9 544,195 - Pf6N2E2_478 0.50 +0.2
Or see this region's nucleotide sequence