Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4660

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C
5,388,957	-		Pf6N2E2_4658	0.50	-1.4
5,389,028	+		Pf6N2E2_4658	0.81	-0.6
5,389,028	+		Pf6N2E2_4658	0.81	+0.0
5,389,028	+		Pf6N2E2_4658	0.81	-0.5
5,389,028	+		Pf6N2E2_4658	0.81	-0.2
5,389,028	+		Pf6N2E2_4658	0.81	-1.0
5,389,028	+		Pf6N2E2_4658	0.81	+0.3
5,389,028	+		Pf6N2E2_4658	0.81	-0.0
5,389,029	-		Pf6N2E2_4658	0.82	-2.4
5,389,029	-		Pf6N2E2_4658	0.82	+0.9
5,389,083	+				+1.1
5,389,083	+				-0.6
5,389,084	-				-0.3
5,389,084	-				-1.7
5,389,084	-				+0.3
5,389,084	-				+0.5
5,389,157	+				+0.3
5,389,157	+				-0.2
5,389,157	+				-0.4
5,389,190	+		Pf6N2E2_4659	0.19	-0.6
5,389,191	-		Pf6N2E2_4659	0.20	+0.2
5,389,357	+		Pf6N2E2_4659	0.69	+0.3
5,389,408	+		Pf6N2E2_4659	0.85	-0.3
5,389,408	+		Pf6N2E2_4659	0.85	-0.5
5,389,408	+		Pf6N2E2_4659	0.85	+1.2
5,389,408	+		Pf6N2E2_4659	0.85	-0.7
5,389,408	+		Pf6N2E2_4659	0.85	+0.7
5,389,408	+		Pf6N2E2_4659	0.85	+0.1
5,389,408	+		Pf6N2E2_4659	0.85	+0.1
5,389,490	+				+1.2
5,389,492	+				+1.3
5,390,683	+				-0.2
5,390,683	+				+0.0
5,390,683	+				-0.4
5,390,684	-				+0.0
5,390,746	+				+0.2
5,390,746	+				+1.3
5,390,746	+				+0.1
5,390,747	-				-0.1
5,390,747	-				-0.2
5,390,747	-				+0.3
5,390,747	-				+1.3
5,390,747	-				-0.4
5,390,747	-				-0.3
5,390,764	-				+0.0
5,390,764	-				+0.2
5,390,772	+				+0.1
5,390,783	-				-0.4
5,390,896	+				+0.6
5,390,897	-				+1.3
5,390,897	-				-0.3
5,390,897	-				-0.0
5,390,907	-				-0.6
5,390,922	+				+2.3
5,390,922	+				+0.6
5,390,923	-				-0.8
5,390,923	-				-0.5
5,390,923	-				+1.3
5,390,923	-				-0.0
5,390,923	-				+0.1
5,390,961	+				+1.8
5,390,961	+				-1.0
5,390,961	+				-1.0
5,390,962	-				-0.3
5,390,974	+				-0.1
5,390,974	+				-1.0
5,390,974	+				+0.3
5,390,975	-				+1.1
5,390,975	-				+1.4
5,390,975	-				+0.3
5,390,975	-				+0.9
5,390,993	+				+1.3
5,390,993	+				-0.2
5,390,994	-				+0.5
5,390,994	-				-1.2
5,390,994	-				+0.5
5,391,034	+				+0.5
5,391,034	+				-0.7
5,391,034	+				+0.3
5,391,034	+				+1.3
5,391,034	+				-0.2
5,391,034	+				+0.5
5,391,034	+				+0.3
5,391,034	+				+0.8
5,391,034	+				+0.6
5,391,034	+				-0.1
5,391,034	+				-0.1
5,391,034	+				+0.6
5,391,034	+				-1.2
5,391,034	+				-0.1
5,391,034	+				-1.7
5,391,035	-				-0.1
5,391,035	-				+1.0
5,391,035	-				-0.4
5,391,035	-				+0.4
5,391,035	-				+0.3
5,391,035	-				-0.0
5,391,035	-				-1.1
5,391,035	-				+0.0
5,391,035	-				-0.6
5,391,035	-				+0.2
5,391,035	-				-0.8
5,391,035	-				-0.6
5,391,035	-				+0.0
5,391,035	-				+0.0
5,391,035	-				-1.2
5,391,038	+				-0.8
5,391,038	+				+2.9
5,391,038	+				+2.1
5,391,038	+				-0.2
5,391,038	+				-1.6
5,391,038	+				+0.7
5,391,038	+				+0.0
5,391,038	+				-0.2
5,391,038	+				+0.3
5,391,038	+				+0.3
5,391,038	+				+0.0
5,391,038	+				-0.2
5,391,038	+				-0.1
5,391,038	+				-1.4
5,391,038	+				+0.1
5,391,039	-				-0.6
5,391,039	-				+0.3
5,391,039	-				-1.2
5,391,039	-				-0.2
5,391,039	-				-0.2
5,391,039	-				+0.0
5,391,039	-				+0.0
5,391,039	-				-0.5
5,391,039	-				+0.3
5,391,039	-				+0.3
5,391,039	-				-0.0
5,391,039	-				+0.5
5,391,039	-				-0.8
5,391,039	-				+0.1
5,391,039	-				+0.9
5,391,039	-				-0.7
5,391,039	-				+0.4
5,391,039	-				+0.6
5,391,039	-				+2.1
5,391,039	-				-0.1
5,391,039	-				-0.9
5,391,039	-				-1.0
5,391,039	-				+0.3
5,391,039	-				-0.7
5,391,039	-				+0.3
5,391,039	-				-0.0
5,391,040	+				-0.1
5,391,040	+				+0.3
5,391,041	-				+0.3
5,391,041	-				-0.6
5,391,041	-				+0.2
5,391,041	-				-0.2
5,391,041	-				-1.7
5,391,041	-				-1.4
5,391,042	+				+1.5
5,391,042	+				+0.1
5,391,042	+				+0.3
5,391,042	+				+0.2
5,391,042	+				+0.3
5,391,043	-				-0.7
5,391,051	+				-0.1
5,391,051	+				-0.8
5,391,051	+				-0.2
5,391,052	-				-1.2
5,391,052	-				-0.7
5,391,088	+				-1.5
5,391,089	-				+0.2
5,391,089	-				-0.7
5,391,089	-				-0.1
5,391,089	-				+0.0
5,391,096	+				-1.2
5,391,097	-				+1.3
5,391,097	-				-1.0
5,391,192	+		Pf6N2E2_4661	0.35	-0.8
5,391,203	+		Pf6N2E2_4661	0.39	+0.3
5,391,212	+		Pf6N2E2_4661	0.42	+1.3
5,391,291	+		Pf6N2E2_4661	0.70	+2.3
5,391,291	+		Pf6N2E2_4661	0.70	+1.3
5,391,291	+		Pf6N2E2_4661	0.70	-0.2
5,391,292	-		Pf6N2E2_4661	0.71	-1.8
5,391,292	-		Pf6N2E2_4661	0.71	-1.7
5,391,292	-		Pf6N2E2_4661	0.71	-0.5
5,391,367	+				+0.3
5,391,367	+				-0.7
5,391,374	-				-2.8
5,391,394	+				-1.0
5,391,395	-				-0.9
5,391,395	-				+1.3
5,391,401	+				-2.4
5,391,424	+				+0.4
5,391,424	+				-1.0
5,391,424	+				-0.5
5,391,424	+				-0.9
5,391,424	+				+0.5
5,391,424	+				-0.7
5,391,424	+				+0.3
5,391,425	-				-0.7
5,391,425	-				-1.6
5,391,425	-				+0.1
5,391,459	-				-1.2
5,391,469	+				+0.3
5,391,562	-				-0.8