Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4049

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C
4,729,294	-		Pf6N2E2_4048	0.30	-2.2
4,729,294	-		Pf6N2E2_4048	0.30	-1.0
4,729,571	-		Pf6N2E2_4048	0.52	-1.8
4,729,571	-		Pf6N2E2_4048	0.52	-0.2
4,729,571	-		Pf6N2E2_4048	0.52	+0.3
4,729,571	-		Pf6N2E2_4048	0.52	-2.7
4,729,730	-		Pf6N2E2_4048	0.65	+0.6
4,729,730	-		Pf6N2E2_4048	0.65	-2.7
4,729,730	-		Pf6N2E2_4048	0.65	-1.8
4,729,730	-		Pf6N2E2_4048	0.65	-4.5
4,729,730	-		Pf6N2E2_4048	0.65	-3.3
4,729,907	-		Pf6N2E2_4048	0.79	-1.8
4,729,907	-		Pf6N2E2_4048	0.79	-2.4
4,729,907	-		Pf6N2E2_4048	0.79	-3.7
4,729,907	-		Pf6N2E2_4048	0.79	+1.3
4,729,907	-		Pf6N2E2_4048	0.79	-4.4
4,729,907	-		Pf6N2E2_4048	0.79	-4.0
4,729,928	-		Pf6N2E2_4048	0.81	-3.1
4,729,952	-		Pf6N2E2_4048	0.83	-2.4
4,729,952	-		Pf6N2E2_4048	0.83	-3.4
4,729,952	-		Pf6N2E2_4048	0.83	-4.8
4,729,976	-		Pf6N2E2_4048	0.85	-1.0
4,730,084	-				-1.2
4,730,084	-				-4.0
4,730,084	-				-0.5
4,730,084	-				-4.0
4,730,207	+				-2.0
4,730,207	+				-1.2
4,730,208	-				+0.4
4,730,208	-				-0.1
4,730,343	-				+0.4
4,730,384	+		Pf6N2E2_4049	0.12	-1.6
4,730,385	-		Pf6N2E2_4049	0.12	-0.2
4,730,601	+		Pf6N2E2_4049	0.36	-1.8
4,730,602	-		Pf6N2E2_4049	0.36	-0.5
4,730,602	-		Pf6N2E2_4049	0.36	-0.1
4,730,711	+		Pf6N2E2_4049	0.47	-0.9
4,730,711	+		Pf6N2E2_4049	0.47	-1.3
4,730,749	+		Pf6N2E2_4049	0.52	-1.6
4,730,749	+		Pf6N2E2_4049	0.52	-0.9
4,730,749	+		Pf6N2E2_4049	0.52	-1.1
4,730,749	+		Pf6N2E2_4049	0.52	-0.8
4,730,750	-		Pf6N2E2_4049	0.52	-0.6
4,730,750	-		Pf6N2E2_4049	0.52	+0.8
4,730,750	-		Pf6N2E2_4049	0.52	-1.5
4,730,795	-		Pf6N2E2_4049	0.57	+1.4
4,730,953	+		Pf6N2E2_4049	0.74	+0.1
4,730,953	+		Pf6N2E2_4049	0.74	-0.0
4,730,954	-		Pf6N2E2_4049	0.74	-1.2
4,730,954	-		Pf6N2E2_4049	0.74	-1.9
4,730,954	-		Pf6N2E2_4049	0.74	-1.1
4,730,954	-		Pf6N2E2_4049	0.74	-2.5
4,730,954	-		Pf6N2E2_4049	0.74	-0.6
4,730,954	-		Pf6N2E2_4049	0.74	-1.4
4,730,956	+		Pf6N2E2_4049	0.74	-2.2
4,730,956	+		Pf6N2E2_4049	0.74	-0.9
4,730,956	+		Pf6N2E2_4049	0.74	-0.1
4,730,957	-		Pf6N2E2_4049	0.74	-1.5
4,730,957	-		Pf6N2E2_4049	0.74	-1.8
4,730,957	-		Pf6N2E2_4049	0.74	-0.8
4,731,190	+				-0.2
4,731,190	+				+0.9
4,731,190	+				-1.0
4,731,190	+				-0.0
4,731,190	+				-1.3
4,731,190	+				+1.4
4,731,191	-				+0.3
4,731,191	-				+1.4
4,731,191	-				-0.9
4,731,191	-				+0.4
4,731,256	+		Pf6N2E2_4050	0.28	+0.5
4,731,308	-		Pf6N2E2_4050	0.44	-0.0
4,731,308	-		Pf6N2E2_4050	0.44	-0.1
4,731,380	+		Pf6N2E2_4050	0.66	-0.2
4,731,562	-				-0.9
4,731,597	+				+1.4
4,731,597	+				+1.4
4,731,597	+				+0.4
4,731,597	+				-0.0
4,731,597	+				+0.4
4,731,597	+				-1.6
4,731,597	+				+0.5
4,731,597	+				-0.8
4,731,597	+				+0.6
4,731,597	+				-2.3
4,731,597	+				+0.4
4,731,597	+				-0.4
4,731,598	-				-0.7
4,731,598	-				+0.1
4,731,598	-				-0.8
4,731,598	-				-1.2
4,731,598	-				-0.6
4,731,616	+				-0.1
4,731,616	+				-0.7
4,731,616	+				+2.9
4,731,616	+				-0.3
4,731,617	-				+1.2
4,731,617	-				+0.7
4,731,617	-				+1.0
4,731,617	-				-0.4
4,731,617	-				+0.6
4,731,665	+				-0.1
4,731,666	-				-0.0
4,731,856	+				-0.4
4,731,856	+				-0.2
4,731,856	+				+0.8
4,731,856	+				-0.1
4,731,857	-				-1.2
4,731,857	-				+1.4
4,731,857	-				-0.2
4,731,857	-				+0.4
4,731,896	+				+0.7
4,731,914	+				+0.1
4,731,920	-				+0.4
4,731,920	-				+0.1
4,731,920	-				+0.5
4,731,941	+				+0.2
4,731,942	-				-0.6
4,731,994	+				+0.1
4,731,995	-				+0.4
4,732,153	+		Pf6N2E2_4052	0.79	-0.3