Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3829 and Pf6N2E2_3830 are separated by 39 nucleotides Pf6N2E2_3830 and Pf6N2E2_3831 are separated by 1 nucleotides Pf6N2E2_3831 and Pf6N2E2_3832 are separated by 182 nucleotides Pf6N2E2_3832 and Pf6N2E2_3833 are separated by 177 nucleotides Pf6N2E2_3833 and Pf6N2E2_3834 overlap by 45 nucleotides
Pf6N2E2_3829 - Protein export cytoplasm chaperone protein (SecB, maintains protein to be exported in unfolded state), at 4,463,887 to 4,464,369
_3829
Pf6N2E2_3830 - Glutaredoxin 3 (Grx3), at 4,464,409 to 4,464,663
_3830
Pf6N2E2_3831 - FIG136845: Rhodanese-related sulfurtransferase, at 4,464,665 to 4,465,078
_3831
Pf6N2E2_3832 - 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (EC 5.4.2.1), at 4,465,261 to 4,466,760
_3832
Pf6N2E2_3833 - hypothetical protein, at 4,466,938 to 4,467,123
_3833
Pf6N2E2_3834 - Lipoprotein NlpD, at 4,467,079 to 4,468,371
_3834
Position (kb)
4465
4466
4467 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4464.299 kb on + strand, within Pf6N2E2_3829 at 4464.299 kb on + strand, within Pf6N2E2_3829 at 4464.299 kb on + strand, within Pf6N2E2_3829 at 4464.299 kb on + strand, within Pf6N2E2_3829 at 4464.299 kb on + strand, within Pf6N2E2_3829 at 4464.300 kb on - strand, within Pf6N2E2_3829 at 4464.300 kb on - strand, within Pf6N2E2_3829 at 4464.300 kb on - strand, within Pf6N2E2_3829 at 4464.300 kb on - strand, within Pf6N2E2_3829 at 4464.300 kb on - strand, within Pf6N2E2_3829 at 4464.383 kb on + strand at 4464.384 kb on - strand at 4464.384 kb on - strand at 4464.384 kb on - strand at 4464.384 kb on - strand at 4464.384 kb on - strand at 4464.435 kb on + strand, within Pf6N2E2_3830 at 4464.605 kb on + strand, within Pf6N2E2_3830 at 4464.605 kb on + strand, within Pf6N2E2_3830 at 4464.605 kb on + strand, within Pf6N2E2_3830 at 4464.605 kb on + strand, within Pf6N2E2_3830 at 4464.606 kb on - strand, within Pf6N2E2_3830 at 4464.606 kb on - strand, within Pf6N2E2_3830 at 4464.606 kb on - strand, within Pf6N2E2_3830 at 4464.623 kb on + strand, within Pf6N2E2_3830 at 4464.632 kb on + strand, within Pf6N2E2_3830 at 4464.704 kb on - strand at 4464.704 kb on - strand at 4465.042 kb on - strand at 4465.100 kb on - strand at 4465.115 kb on - strand at 4465.115 kb on - strand at 4465.116 kb on + strand at 4465.116 kb on + strand at 4465.116 kb on + strand at 4465.116 kb on + strand at 4465.116 kb on + strand at 4465.116 kb on + strand at 4465.117 kb on - strand at 4465.117 kb on - strand at 4465.117 kb on - strand at 4465.117 kb on - strand at 4465.117 kb on - strand at 4465.117 kb on - strand at 4465.170 kb on + strand at 4465.170 kb on + strand at 4465.176 kb on + strand at 4465.177 kb on - strand at 4465.236 kb on + strand at 4466.769 kb on + strand at 4466.770 kb on - strand at 4466.770 kb on - strand at 4466.770 kb on - strand at 4466.770 kb on - strand at 4466.880 kb on + strand at 4467.042 kb on + strand, within Pf6N2E2_3833 at 4467.042 kb on + strand, within Pf6N2E2_3833 at 4467.043 kb on - strand, within Pf6N2E2_3833 at 4467.043 kb on - strand, within Pf6N2E2_3833 at 4467.045 kb on + strand, within Pf6N2E2_3833 at 4467.045 kb on + strand, within Pf6N2E2_3833 at 4467.046 kb on - strand, within Pf6N2E2_3833 at 4467.046 kb on - strand, within Pf6N2E2_3833 at 4467.046 kb on - strand, within Pf6N2E2_3833 at 4467.046 kb on - strand, within Pf6N2E2_3833 at 4467.046 kb on - strand, within Pf6N2E2_3833 at 4467.078 kb on + strand, within Pf6N2E2_3833 at 4467.079 kb on - strand at 4467.079 kb on - strand at 4467.440 kb on - strand, within Pf6N2E2_3834 at 4467.440 kb on - strand, within Pf6N2E2_3834 at 4467.496 kb on + strand, within Pf6N2E2_3834 at 4467.497 kb on - strand, within Pf6N2E2_3834 at 4467.497 kb on - strand, within Pf6N2E2_3834 at 4467.499 kb on + strand, within Pf6N2E2_3834 at 4467.499 kb on + strand, within Pf6N2E2_3834 at 4467.500 kb on - strand, within Pf6N2E2_3834 at 4467.500 kb on - strand, within Pf6N2E2_3834
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C remove 4,464,299 + Pf6N2E2_3829 0.85 -1.2 4,464,299 + Pf6N2E2_3829 0.85 +1.4 4,464,299 + Pf6N2E2_3829 0.85 +0.9 4,464,299 + Pf6N2E2_3829 0.85 -0.5 4,464,299 + Pf6N2E2_3829 0.85 +0.7 4,464,300 - Pf6N2E2_3829 0.86 +0.1 4,464,300 - Pf6N2E2_3829 0.86 +0.4 4,464,300 - Pf6N2E2_3829 0.86 +0.1 4,464,300 - Pf6N2E2_3829 0.86 +1.4 4,464,300 - Pf6N2E2_3829 0.86 -0.2 4,464,383 + +1.2 4,464,384 - -0.2 4,464,384 - -0.1 4,464,384 - +0.1 4,464,384 - +1.3 4,464,384 - +0.5 4,464,435 + Pf6N2E2_3830 0.10 -0.1 4,464,605 + Pf6N2E2_3830 0.77 +1.7 4,464,605 + Pf6N2E2_3830 0.77 -0.2 4,464,605 + Pf6N2E2_3830 0.77 +0.5 4,464,605 + Pf6N2E2_3830 0.77 +1.1 4,464,606 - Pf6N2E2_3830 0.77 -1.2 4,464,606 - Pf6N2E2_3830 0.77 +1.2 4,464,606 - Pf6N2E2_3830 0.77 +0.8 4,464,623 + Pf6N2E2_3830 0.84 +0.8 4,464,632 + Pf6N2E2_3830 0.87 +0.3 4,464,704 - +0.2 4,464,704 - +1.0 4,465,042 - +0.9 4,465,100 - -0.4 4,465,115 - -0.2 4,465,115 - +0.0 4,465,116 + +0.6 4,465,116 + +0.3 4,465,116 + -1.6 4,465,116 + +0.2 4,465,116 + +1.8 4,465,116 + -0.5 4,465,117 - +0.3 4,465,117 - +0.6 4,465,117 - +0.0 4,465,117 - +0.1 4,465,117 - +2.0 4,465,117 - +0.6 4,465,170 + -0.3 4,465,170 + -0.3 4,465,176 + -0.1 4,465,177 - -1.6 4,465,236 + +1.3 4,466,769 + +0.5 4,466,770 - -0.1 4,466,770 - -0.3 4,466,770 - +0.1 4,466,770 - +0.4 4,466,880 + +0.6 4,467,042 + Pf6N2E2_3833 0.56 +0.6 4,467,042 + Pf6N2E2_3833 0.56 +0.3 4,467,043 - Pf6N2E2_3833 0.56 +2.3 4,467,043 - Pf6N2E2_3833 0.56 +1.3 4,467,045 + Pf6N2E2_3833 0.58 +0.3 4,467,045 + Pf6N2E2_3833 0.58 -0.5 4,467,046 - Pf6N2E2_3833 0.58 +1.3 4,467,046 - Pf6N2E2_3833 0.58 +1.3 4,467,046 - Pf6N2E2_3833 0.58 -2.5 4,467,046 - Pf6N2E2_3833 0.58 +0.7 4,467,046 - Pf6N2E2_3833 0.58 +1.3 4,467,078 + Pf6N2E2_3833 0.75 -1.2 4,467,079 - +0.1 4,467,079 - +2.1 4,467,440 - Pf6N2E2_3834 0.28 -0.3 4,467,440 - Pf6N2E2_3834 0.28 +2.2 4,467,496 + Pf6N2E2_3834 0.32 +0.8 4,467,497 - Pf6N2E2_3834 0.32 +1.3 4,467,497 - Pf6N2E2_3834 0.32 +1.3 4,467,499 + Pf6N2E2_3834 0.32 -0.7 4,467,499 + Pf6N2E2_3834 0.32 +0.3 4,467,500 - Pf6N2E2_3834 0.33 +1.5 4,467,500 - Pf6N2E2_3834 0.33 +0.3
Or see this region's nucleotide sequence