Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3452 and Pf6N2E2_3453 are separated by 139 nucleotides Pf6N2E2_3453 and Pf6N2E2_3454 are separated by 185 nucleotides
Pf6N2E2_3452 - Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27), at 4,019,937 to 4,021,463
_3452
Pf6N2E2_3453 - Transcriptional regulator, LysR family, at 4,021,603 to 4,022,550
_3453
Pf6N2E2_3454 - hypothetical protein, at 4,022,736 to 4,024,052
_3454
Position (kb)
4021
4022
4023 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4020.730 kb on + strand, within Pf6N2E2_3452 at 4020.730 kb on + strand, within Pf6N2E2_3452 at 4020.730 kb on + strand, within Pf6N2E2_3452 at 4020.730 kb on + strand, within Pf6N2E2_3452 at 4020.730 kb on + strand, within Pf6N2E2_3452 at 4020.730 kb on + strand, within Pf6N2E2_3452 at 4020.730 kb on + strand, within Pf6N2E2_3452 at 4020.731 kb on - strand, within Pf6N2E2_3452 at 4020.926 kb on + strand, within Pf6N2E2_3452 at 4020.926 kb on + strand, within Pf6N2E2_3452 at 4020.926 kb on + strand, within Pf6N2E2_3452 at 4020.926 kb on + strand, within Pf6N2E2_3452 at 4020.927 kb on - strand, within Pf6N2E2_3452 at 4020.993 kb on - strand, within Pf6N2E2_3452 at 4020.993 kb on - strand, within Pf6N2E2_3452 at 4021.030 kb on + strand, within Pf6N2E2_3452 at 4021.030 kb on + strand, within Pf6N2E2_3452 at 4021.030 kb on + strand, within Pf6N2E2_3452 at 4021.030 kb on + strand, within Pf6N2E2_3452 at 4021.030 kb on + strand, within Pf6N2E2_3452 at 4021.030 kb on + strand, within Pf6N2E2_3452 at 4021.030 kb on + strand, within Pf6N2E2_3452 at 4021.031 kb on - strand, within Pf6N2E2_3452 at 4021.031 kb on - strand, within Pf6N2E2_3452 at 4021.031 kb on - strand, within Pf6N2E2_3452 at 4021.031 kb on - strand, within Pf6N2E2_3452 at 4021.449 kb on - strand at 4021.485 kb on + strand at 4021.485 kb on + strand at 4021.486 kb on - strand at 4021.486 kb on - strand at 4021.486 kb on - strand at 4021.487 kb on + strand at 4021.487 kb on + strand at 4021.556 kb on + strand at 4021.556 kb on + strand at 4021.556 kb on + strand at 4021.556 kb on + strand at 4021.556 kb on + strand at 4021.557 kb on - strand at 4021.557 kb on - strand at 4021.557 kb on - strand at 4021.557 kb on - strand at 4021.557 kb on - strand at 4021.557 kb on - strand at 4021.573 kb on - strand at 4021.717 kb on + strand, within Pf6N2E2_3453 at 4021.718 kb on - strand, within Pf6N2E2_3453 at 4021.913 kb on + strand, within Pf6N2E2_3453 at 4021.914 kb on - strand, within Pf6N2E2_3453 at 4021.914 kb on - strand, within Pf6N2E2_3453 at 4021.914 kb on - strand, within Pf6N2E2_3453 at 4021.914 kb on - strand, within Pf6N2E2_3453 at 4021.914 kb on - strand, within Pf6N2E2_3453 at 4022.089 kb on + strand, within Pf6N2E2_3453 at 4022.089 kb on + strand, within Pf6N2E2_3453 at 4022.089 kb on + strand, within Pf6N2E2_3453 at 4022.093 kb on + strand, within Pf6N2E2_3453 at 4022.093 kb on + strand, within Pf6N2E2_3453 at 4022.093 kb on + strand, within Pf6N2E2_3453 at 4022.093 kb on + strand, within Pf6N2E2_3453 at 4022.094 kb on - strand, within Pf6N2E2_3453 at 4022.094 kb on - strand, within Pf6N2E2_3453 at 4022.094 kb on - strand, within Pf6N2E2_3453 at 4022.094 kb on - strand, within Pf6N2E2_3453 at 4022.094 kb on - strand, within Pf6N2E2_3453 at 4022.094 kb on - strand, within Pf6N2E2_3453 at 4022.094 kb on - strand, within Pf6N2E2_3453 at 4022.094 kb on - strand, within Pf6N2E2_3453 at 4022.094 kb on - strand, within Pf6N2E2_3453 at 4022.094 kb on - strand, within Pf6N2E2_3453 at 4022.094 kb on - strand, within Pf6N2E2_3453 at 4022.143 kb on + strand, within Pf6N2E2_3453 at 4022.144 kb on - strand, within Pf6N2E2_3453 at 4022.144 kb on - strand, within Pf6N2E2_3453 at 4022.206 kb on + strand, within Pf6N2E2_3453 at 4022.206 kb on + strand, within Pf6N2E2_3453 at 4022.207 kb on - strand, within Pf6N2E2_3453 at 4022.242 kb on + strand, within Pf6N2E2_3453 at 4022.242 kb on + strand, within Pf6N2E2_3453 at 4022.242 kb on + strand, within Pf6N2E2_3453 at 4022.242 kb on + strand, within Pf6N2E2_3453 at 4022.243 kb on - strand, within Pf6N2E2_3453 at 4022.426 kb on - strand, within Pf6N2E2_3453 at 4022.460 kb on + strand at 4022.460 kb on + strand at 4022.460 kb on + strand at 4022.460 kb on + strand at 4022.460 kb on + strand at 4022.476 kb on + strand at 4022.476 kb on + strand at 4022.606 kb on + strand at 4022.606 kb on + strand at 4022.606 kb on + strand at 4022.607 kb on - strand at 4022.607 kb on - strand at 4022.612 kb on + strand at 4022.612 kb on + strand at 4022.612 kb on + strand at 4022.612 kb on + strand at 4022.613 kb on - strand at 4022.615 kb on + strand at 4022.615 kb on + strand at 4022.615 kb on + strand at 4022.615 kb on + strand at 4022.633 kb on - strand at 4022.675 kb on - strand at 4022.681 kb on + strand at 4022.681 kb on + strand at 4022.681 kb on + strand at 4022.681 kb on + strand at 4022.681 kb on + strand at 4022.681 kb on + strand at 4022.724 kb on + strand at 4023.402 kb on - strand, within Pf6N2E2_3454
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C remove 4,020,730 + Pf6N2E2_3452 0.52 -0.3 4,020,730 + Pf6N2E2_3452 0.52 -1.5 4,020,730 + Pf6N2E2_3452 0.52 -0.3 4,020,730 + Pf6N2E2_3452 0.52 -0.0 4,020,730 + Pf6N2E2_3452 0.52 -0.3 4,020,730 + Pf6N2E2_3452 0.52 +0.5 4,020,730 + Pf6N2E2_3452 0.52 -0.6 4,020,731 - Pf6N2E2_3452 0.52 +0.1 4,020,926 + Pf6N2E2_3452 0.65 +0.1 4,020,926 + Pf6N2E2_3452 0.65 -0.1 4,020,926 + Pf6N2E2_3452 0.65 +0.7 4,020,926 + Pf6N2E2_3452 0.65 -1.1 4,020,927 - Pf6N2E2_3452 0.65 -0.3 4,020,993 - Pf6N2E2_3452 0.69 +1.3 4,020,993 - Pf6N2E2_3452 0.69 -0.2 4,021,030 + Pf6N2E2_3452 0.72 -0.2 4,021,030 + Pf6N2E2_3452 0.72 -0.5 4,021,030 + Pf6N2E2_3452 0.72 +0.7 4,021,030 + Pf6N2E2_3452 0.72 +2.3 4,021,030 + Pf6N2E2_3452 0.72 +0.1 4,021,030 + Pf6N2E2_3452 0.72 -1.0 4,021,030 + Pf6N2E2_3452 0.72 -0.7 4,021,031 - Pf6N2E2_3452 0.72 -0.6 4,021,031 - Pf6N2E2_3452 0.72 +0.6 4,021,031 - Pf6N2E2_3452 0.72 -0.5 4,021,031 - Pf6N2E2_3452 0.72 -0.9 4,021,449 - +0.1 4,021,485 + -2.2 4,021,485 + -2.1 4,021,486 - -0.2 4,021,486 - +2.3 4,021,486 - +0.5 4,021,487 + -0.1 4,021,487 + -0.6 4,021,556 + -1.4 4,021,556 + -0.1 4,021,556 + -1.9 4,021,556 + -0.6 4,021,556 + -0.7 4,021,557 - +0.2 4,021,557 - +0.0 4,021,557 - -0.0 4,021,557 - +0.3 4,021,557 - +1.6 4,021,557 - -0.2 4,021,573 - +0.1 4,021,717 + Pf6N2E2_3453 0.12 -0.2 4,021,718 - Pf6N2E2_3453 0.12 +1.0 4,021,913 + Pf6N2E2_3453 0.33 +1.3 4,021,914 - Pf6N2E2_3453 0.33 -2.5 4,021,914 - Pf6N2E2_3453 0.33 +0.3 4,021,914 - Pf6N2E2_3453 0.33 +0.3 4,021,914 - Pf6N2E2_3453 0.33 -0.1 4,021,914 - Pf6N2E2_3453 0.33 -0.4 4,022,089 + Pf6N2E2_3453 0.51 -1.1 4,022,089 + Pf6N2E2_3453 0.51 +0.4 4,022,089 + Pf6N2E2_3453 0.51 -0.4 4,022,093 + Pf6N2E2_3453 0.52 -1.0 4,022,093 + Pf6N2E2_3453 0.52 -0.6 4,022,093 + Pf6N2E2_3453 0.52 +0.3 4,022,093 + Pf6N2E2_3453 0.52 +1.3 4,022,094 - Pf6N2E2_3453 0.52 +0.3 4,022,094 - Pf6N2E2_3453 0.52 -0.6 4,022,094 - Pf6N2E2_3453 0.52 -0.3 4,022,094 - Pf6N2E2_3453 0.52 +0.5 4,022,094 - Pf6N2E2_3453 0.52 +0.3 4,022,094 - Pf6N2E2_3453 0.52 +0.0 4,022,094 - Pf6N2E2_3453 0.52 +0.1 4,022,094 - Pf6N2E2_3453 0.52 -0.3 4,022,094 - Pf6N2E2_3453 0.52 -0.1 4,022,094 - Pf6N2E2_3453 0.52 +0.5 4,022,094 - Pf6N2E2_3453 0.52 -0.9 4,022,143 + Pf6N2E2_3453 0.57 +0.3 4,022,144 - Pf6N2E2_3453 0.57 +0.3 4,022,144 - Pf6N2E2_3453 0.57 +0.4 4,022,206 + Pf6N2E2_3453 0.64 -0.8 4,022,206 + Pf6N2E2_3453 0.64 -0.7 4,022,207 - Pf6N2E2_3453 0.64 -0.7 4,022,242 + Pf6N2E2_3453 0.67 +1.3 4,022,242 + Pf6N2E2_3453 0.67 -0.8 4,022,242 + Pf6N2E2_3453 0.67 -2.0 4,022,242 + Pf6N2E2_3453 0.67 -2.8 4,022,243 - Pf6N2E2_3453 0.68 -0.3 4,022,426 - Pf6N2E2_3453 0.87 -0.7 4,022,460 + -3.7 4,022,460 + -1.8 4,022,460 + +0.3 4,022,460 + -3.8 4,022,460 + -3.0 4,022,476 + -1.6 4,022,476 + -3.2 4,022,606 + -3.9 4,022,606 + -2.0 4,022,606 + -2.6 4,022,607 - -0.1 4,022,607 - +0.3 4,022,612 + -1.3 4,022,612 + -2.8 4,022,612 + -2.6 4,022,612 + -2.1 4,022,613 - -0.7 4,022,615 + -0.7 4,022,615 + -4.1 4,022,615 + +0.3 4,022,615 + -1.5 4,022,633 - +1.3 4,022,675 - -0.3 4,022,681 + +0.3 4,022,681 + -3.6 4,022,681 + -1.7 4,022,681 + -1.7 4,022,681 + -3.0 4,022,681 + -3.8 4,022,724 + -3.8 4,023,402 - Pf6N2E2_3454 0.51 +1.3
Or see this region's nucleotide sequence