Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3356

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3355 and Pf6N2E2_6118 are separated by 143 nucleotidesPf6N2E2_6118 and Pf6N2E2_6119 are separated by 100 nucleotidesPf6N2E2_6119 and Pf6N2E2_3356 are separated by 409 nucleotidesPf6N2E2_3356 and Pf6N2E2_3357 are separated by 78 nucleotides Pf6N2E2_3355 - FIG000325: clustered with transcription termination protein NusA, at 3,885,341 to 3,885,799 _3355 Pf6N2E2_6118 - tRNA-Met-CAT, at 3,885,943 to 3,886,016 _6118 Pf6N2E2_6119 - tRNA-Leu-GAG, at 3,886,117 to 3,886,199 _6119 Pf6N2E2_3356 - Triosephosphate isomerase (EC 5.3.1.1), at 3,886,609 to 3,887,352 _3356 Pf6N2E2_3357 - Phosphoglucosamine mutase (EC 5.4.2.10), at 3,887,431 to 3,888,768 _3357 Position (kb) 3886 3887 3888Strain fitness (log2 ratio) -2 -1 0 1at 3885.894 kb on - strandat 3885.894 kb on - strandat 3885.894 kb on - strandat 3885.894 kb on - strandat 3886.036 kb on - strandat 3886.036 kb on - strandat 3886.038 kb on - strandat 3886.038 kb on - strandat 3886.052 kb on - strandat 3886.052 kb on - strandat 3886.052 kb on - strandat 3886.078 kb on - strandat 3886.111 kb on - strandat 3886.111 kb on - strandat 3886.223 kb on - strandat 3886.246 kb on - strandat 3886.317 kb on - strandat 3886.317 kb on - strandat 3886.330 kb on - strandat 3887.448 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C
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3,885,894 - -0.0
3,885,894 - +1.3
3,885,894 - -0.1
3,885,894 - -0.1
3,886,036 - -0.5
3,886,036 - +0.2
3,886,038 - -0.4
3,886,038 - -0.2
3,886,052 - -0.4
3,886,052 - -0.6
3,886,052 - -0.4
3,886,078 - +0.0
3,886,111 - -1.3
3,886,111 - -1.2
3,886,223 - -0.4
3,886,246 - -1.1
3,886,317 - +1.3
3,886,317 - -1.8
3,886,330 - -0.2
3,887,448 + +1.2

Or see this region's nucleotide sequence