Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3276 and Pf6N2E2_3277 overlap by 8 nucleotides Pf6N2E2_3277 and Pf6N2E2_3278 are separated by 284 nucleotides Pf6N2E2_3278 and Pf6N2E2_3279 are separated by 96 nucleotides Pf6N2E2_3279 and Pf6N2E2_3280 are separated by 148 nucleotides
Pf6N2E2_3276 - Fumarate hydratase class II (EC 4.2.1.2), at 3,779,056 to 3,780,432
_3276
Pf6N2E2_3277 - FagA protein, at 3,780,425 to 3,780,820
_3277
Pf6N2E2_3278 - TldE protein, part of TldE/TldD proteolytic complex, at 3,781,105 to 3,782,463
_3278
Pf6N2E2_3279 - FIG138315: Putative alpha helix protein, at 3,782,560 to 3,783,084
_3279
Pf6N2E2_3280 - TldD protein, part of TldE/TldD proteolytic complex, at 3,783,233 to 3,784,675
_3280
Position (kb)
3781
3782
3783 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3780.194 kb on - strand, within Pf6N2E2_3276 at 3780.253 kb on + strand, within Pf6N2E2_3276 at 3780.254 kb on - strand, within Pf6N2E2_3276 at 3780.254 kb on - strand, within Pf6N2E2_3276 at 3780.254 kb on - strand, within Pf6N2E2_3276 at 3780.315 kb on + strand at 3780.315 kb on + strand at 3780.316 kb on - strand at 3780.316 kb on - strand at 3780.316 kb on - strand at 3780.424 kb on - strand at 3780.424 kb on - strand at 3780.424 kb on - strand at 3780.426 kb on + strand at 3780.426 kb on + strand at 3780.426 kb on + strand at 3780.426 kb on + strand at 3780.427 kb on - strand at 3780.427 kb on - strand at 3780.427 kb on - strand at 3780.427 kb on - strand at 3780.427 kb on - strand at 3780.457 kb on + strand at 3780.458 kb on - strand at 3780.458 kb on - strand at 3780.458 kb on - strand at 3780.714 kb on + strand, within Pf6N2E2_3277 at 3780.714 kb on + strand, within Pf6N2E2_3277 at 3780.715 kb on - strand, within Pf6N2E2_3277 at 3780.792 kb on + strand at 3780.792 kb on + strand at 3780.793 kb on - strand at 3780.793 kb on - strand at 3780.793 kb on - strand at 3780.950 kb on + strand at 3781.189 kb on + strand at 3781.216 kb on - strand at 3781.295 kb on + strand, within Pf6N2E2_3278 at 3781.296 kb on - strand, within Pf6N2E2_3278 at 3781.296 kb on - strand, within Pf6N2E2_3278 at 3781.410 kb on + strand, within Pf6N2E2_3278 at 3781.411 kb on - strand, within Pf6N2E2_3278 at 3781.463 kb on + strand, within Pf6N2E2_3278 at 3781.463 kb on + strand, within Pf6N2E2_3278 at 3781.463 kb on + strand, within Pf6N2E2_3278 at 3781.464 kb on - strand, within Pf6N2E2_3278 at 3781.478 kb on + strand, within Pf6N2E2_3278 at 3781.478 kb on + strand, within Pf6N2E2_3278 at 3781.478 kb on + strand, within Pf6N2E2_3278 at 3781.478 kb on + strand, within Pf6N2E2_3278 at 3781.478 kb on + strand, within Pf6N2E2_3278 at 3781.478 kb on + strand, within Pf6N2E2_3278 at 3781.479 kb on - strand, within Pf6N2E2_3278 at 3781.479 kb on - strand, within Pf6N2E2_3278 at 3781.517 kb on + strand, within Pf6N2E2_3278 at 3781.517 kb on + strand, within Pf6N2E2_3278 at 3781.518 kb on - strand, within Pf6N2E2_3278 at 3781.518 kb on - strand, within Pf6N2E2_3278 at 3781.518 kb on - strand, within Pf6N2E2_3278 at 3781.839 kb on - strand, within Pf6N2E2_3278 at 3781.913 kb on + strand, within Pf6N2E2_3278 at 3781.944 kb on - strand, within Pf6N2E2_3278 at 3781.944 kb on - strand, within Pf6N2E2_3278 at 3781.966 kb on - strand, within Pf6N2E2_3278 at 3782.163 kb on + strand, within Pf6N2E2_3278 at 3782.164 kb on - strand, within Pf6N2E2_3278 at 3782.246 kb on + strand, within Pf6N2E2_3278 at 3782.246 kb on + strand, within Pf6N2E2_3278 at 3782.246 kb on + strand, within Pf6N2E2_3278 at 3782.247 kb on - strand, within Pf6N2E2_3278 at 3782.247 kb on - strand, within Pf6N2E2_3278 at 3782.247 kb on - strand, within Pf6N2E2_3278 at 3782.247 kb on - strand, within Pf6N2E2_3278 at 3782.247 kb on - strand, within Pf6N2E2_3278 at 3782.247 kb on - strand, within Pf6N2E2_3278 at 3782.339 kb on - strand at 3782.339 kb on - strand at 3782.587 kb on + strand at 3782.854 kb on + strand, within Pf6N2E2_3279 at 3782.855 kb on - strand, within Pf6N2E2_3279 at 3782.855 kb on - strand, within Pf6N2E2_3279 at 3782.855 kb on - strand, within Pf6N2E2_3279 at 3782.966 kb on + strand, within Pf6N2E2_3279 at 3782.966 kb on + strand, within Pf6N2E2_3279 at 3782.966 kb on + strand, within Pf6N2E2_3279 at 3782.967 kb on - strand, within Pf6N2E2_3279 at 3782.967 kb on - strand, within Pf6N2E2_3279 at 3782.967 kb on - strand, within Pf6N2E2_3279 at 3783.043 kb on + strand at 3783.043 kb on + strand at 3783.043 kb on + strand at 3783.111 kb on - strand at 3783.111 kb on - strand at 3783.180 kb on - strand at 3783.186 kb on + strand at 3783.187 kb on - strand at 3783.450 kb on + strand, within Pf6N2E2_3280 at 3783.451 kb on - strand, within Pf6N2E2_3280 at 3783.451 kb on - strand, within Pf6N2E2_3280
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C remove 3,780,194 - Pf6N2E2_3276 0.83 +1.4 3,780,253 + Pf6N2E2_3276 0.87 +0.0 3,780,254 - Pf6N2E2_3276 0.87 -0.6 3,780,254 - Pf6N2E2_3276 0.87 +0.1 3,780,254 - Pf6N2E2_3276 0.87 +0.1 3,780,315 + -0.0 3,780,315 + -2.9 3,780,316 - +0.2 3,780,316 - +0.7 3,780,316 - -0.8 3,780,424 - -0.3 3,780,424 - -0.1 3,780,424 - +0.1 3,780,426 + +1.4 3,780,426 + +0.6 3,780,426 + +0.8 3,780,426 + -0.0 3,780,427 - -0.6 3,780,427 - +0.4 3,780,427 - +0.4 3,780,427 - +0.4 3,780,427 - +0.5 3,780,457 + -0.3 3,780,458 - +1.0 3,780,458 - +0.4 3,780,458 - +0.0 3,780,714 + Pf6N2E2_3277 0.73 +0.2 3,780,714 + Pf6N2E2_3277 0.73 -0.4 3,780,715 - Pf6N2E2_3277 0.73 +0.6 3,780,792 + +0.9 3,780,792 + +0.2 3,780,793 - +1.4 3,780,793 - +0.7 3,780,793 - +0.6 3,780,950 + -2.1 3,781,189 + -0.9 3,781,216 - -1.7 3,781,295 + Pf6N2E2_3278 0.14 -1.1 3,781,296 - Pf6N2E2_3278 0.14 -2.8 3,781,296 - Pf6N2E2_3278 0.14 +1.4 3,781,410 + Pf6N2E2_3278 0.22 -0.4 3,781,411 - Pf6N2E2_3278 0.23 -0.6 3,781,463 + Pf6N2E2_3278 0.26 -0.2 3,781,463 + Pf6N2E2_3278 0.26 -1.2 3,781,463 + Pf6N2E2_3278 0.26 +0.2 3,781,464 - Pf6N2E2_3278 0.26 +0.4 3,781,478 + Pf6N2E2_3278 0.27 -1.0 3,781,478 + Pf6N2E2_3278 0.27 -0.9 3,781,478 + Pf6N2E2_3278 0.27 -1.1 3,781,478 + Pf6N2E2_3278 0.27 -1.8 3,781,478 + Pf6N2E2_3278 0.27 -0.1 3,781,478 + Pf6N2E2_3278 0.27 +0.0 3,781,479 - Pf6N2E2_3278 0.28 +0.0 3,781,479 - Pf6N2E2_3278 0.28 +0.0 3,781,517 + Pf6N2E2_3278 0.30 -2.6 3,781,517 + Pf6N2E2_3278 0.30 -0.6 3,781,518 - Pf6N2E2_3278 0.30 -1.1 3,781,518 - Pf6N2E2_3278 0.30 -1.0 3,781,518 - Pf6N2E2_3278 0.30 -0.6 3,781,839 - Pf6N2E2_3278 0.54 -1.0 3,781,913 + Pf6N2E2_3278 0.59 -0.5 3,781,944 - Pf6N2E2_3278 0.62 -0.7 3,781,944 - Pf6N2E2_3278 0.62 -0.7 3,781,966 - Pf6N2E2_3278 0.63 +0.2 3,782,163 + Pf6N2E2_3278 0.78 -0.9 3,782,164 - Pf6N2E2_3278 0.78 -0.2 3,782,246 + Pf6N2E2_3278 0.84 -0.9 3,782,246 + Pf6N2E2_3278 0.84 +0.5 3,782,246 + Pf6N2E2_3278 0.84 -1.2 3,782,247 - Pf6N2E2_3278 0.84 -0.6 3,782,247 - Pf6N2E2_3278 0.84 -0.9 3,782,247 - Pf6N2E2_3278 0.84 -0.2 3,782,247 - Pf6N2E2_3278 0.84 -1.6 3,782,247 - Pf6N2E2_3278 0.84 -0.1 3,782,247 - Pf6N2E2_3278 0.84 -1.0 3,782,339 - -0.9 3,782,339 - -0.8 3,782,587 + +1.4 3,782,854 + Pf6N2E2_3279 0.56 -0.9 3,782,855 - Pf6N2E2_3279 0.56 +0.4 3,782,855 - Pf6N2E2_3279 0.56 +0.4 3,782,855 - Pf6N2E2_3279 0.56 +0.7 3,782,966 + Pf6N2E2_3279 0.77 +1.1 3,782,966 + Pf6N2E2_3279 0.77 -0.5 3,782,966 + Pf6N2E2_3279 0.77 -0.2 3,782,967 - Pf6N2E2_3279 0.78 +2.4 3,782,967 - Pf6N2E2_3279 0.78 -0.2 3,782,967 - Pf6N2E2_3279 0.78 +0.7 3,783,043 + +0.2 3,783,043 + +1.4 3,783,043 + -0.2 3,783,111 - +0.3 3,783,111 - +2.4 3,783,180 - -0.2 3,783,186 + +1.4 3,783,187 - +0.0 3,783,450 + Pf6N2E2_3280 0.15 -1.1 3,783,451 - Pf6N2E2_3280 0.15 -0.1 3,783,451 - Pf6N2E2_3280 0.15 -1.6
Or see this region's nucleotide sequence